目的观察吉祥草中甾体皂苷RCE-4对宫颈癌裸鼠移植瘤的抑制作用。方法采用传统方法分离鉴定RCE-4。将0.3 m L Caski(4×107/m L)细胞悬液接种于裸鼠右侧后腿背部皮下,建立宫颈癌移植瘤模型,然后按瘤体积大小随机分为模型组、紫杉醇(1...目的观察吉祥草中甾体皂苷RCE-4对宫颈癌裸鼠移植瘤的抑制作用。方法采用传统方法分离鉴定RCE-4。将0.3 m L Caski(4×107/m L)细胞悬液接种于裸鼠右侧后腿背部皮下,建立宫颈癌移植瘤模型,然后按瘤体积大小随机分为模型组、紫杉醇(10 mg/kg)和RCE-4(25、50、100 mg/kg)组,1次/d给药,连续4周,末次给药的次日处死裸鼠,称量裸鼠体质量、瘤质量,测量肿瘤体积,计算抑瘤率。光镜下观察移植瘤组织形态学变化,TUNEL染色测定细胞凋亡率,免疫组化检测肿瘤组织中COX-2表达,荧光定量PCR测定移植瘤组织中Bcl-2、Bax、Caspase-3和Caspase-9 mRNA表达,计算Bcl-2/Bax值,Western blot检测移植瘤组织中Survivin蛋白表达。结果 RCE-4(25、50、100 mg/kg)可明显降低宫颈癌裸鼠移植瘤的体积和瘤质量(P<0.05,P<0.01),上调Bax、Caspase-3和Caspase-9 mRNA表达,下调Bcl-2 mRNA表达和COX-2、Survivin蛋白表达及Bcl-2/Bax值(P<0.05,P<0.01)。结论RCE-4对宫颈癌裸鼠移植瘤的生长具有显著抑制作用,其机制可能与其上调Bax、Caspase-9、Caspase-3的表达和下调Survivin、Bcl-2、COX-2的表达有关。展开更多
采用配有 dc- N plasma N源的分子束外延 (MBE)技术在 Ga As衬底上生长制作了工作波长为 1 ,3 μm的 Ga In NAs量子阱 RCE探测器 .采用传输矩阵法对器件结构进行优化 .吸收区由三个 Ga In NAs量子阱构成 ,并用湿法刻蚀和聚酰亚胺对器件...采用配有 dc- N plasma N源的分子束外延 (MBE)技术在 Ga As衬底上生长制作了工作波长为 1 ,3 μm的 Ga In NAs量子阱 RCE探测器 .采用传输矩阵法对器件结构进行优化 .吸收区由三个 Ga In NAs量子阱构成 ,并用湿法刻蚀和聚酰亚胺对器件进行隔离 .在零偏压下 ,器件最大的量子效率为 1 2 %,半峰值全宽 (FWHM)为 5 .8nm,3 d B带宽为 3 0 MHz,暗电流为 2× 1 0 - 11A.通过对 MBE生长条件和器件结构的优化 ,将进一步提高该器件的性能 .展开更多
There are more than 30 fossil-dominated geoparks established in China.Most of them are of high scientific values.Compared to landscape-dominated geoparks,the fossil-dominated ones generally lack of charming natural sc...There are more than 30 fossil-dominated geoparks established in China.Most of them are of high scientific values.Compared to landscape-dominated geoparks,the fossil-dominated ones generally lack of charming natural sceneries,but the abundant and exquisite preserved fossils in geoparks are very attractive to the public.In order to protect and exploit these fossils,the evaluation plays an important role in the geopark construction.Ningcheng geopark is famous for the Mesozoic Daohugou biota.The fossil assemblage is different from Jehol biota,which attracts many experts.In this paper the展开更多
基金Major State Basic Research Program under Grant No.G2 0 0 0 0 36 6 0 3theNational Natural Science Foundation of China under Grant Nos.6 9896 2 6 0 +1 种基金6 99880 0 5 6 9976 0 0 7and6 978980 2
文摘采用配有 dc- N plasma N源的分子束外延 (MBE)技术在 Ga As衬底上生长制作了工作波长为 1 ,3 μm的 Ga In NAs量子阱 RCE探测器 .采用传输矩阵法对器件结构进行优化 .吸收区由三个 Ga In NAs量子阱构成 ,并用湿法刻蚀和聚酰亚胺对器件进行隔离 .在零偏压下 ,器件最大的量子效率为 1 2 %,半峰值全宽 (FWHM)为 5 .8nm,3 d B带宽为 3 0 MHz,暗电流为 2× 1 0 - 11A.通过对 MBE生长条件和器件结构的优化 ,将进一步提高该器件的性能 .
文摘There are more than 30 fossil-dominated geoparks established in China.Most of them are of high scientific values.Compared to landscape-dominated geoparks,the fossil-dominated ones generally lack of charming natural sceneries,but the abundant and exquisite preserved fossils in geoparks are very attractive to the public.In order to protect and exploit these fossils,the evaluation plays an important role in the geopark construction.Ningcheng geopark is famous for the Mesozoic Daohugou biota.The fossil assemblage is different from Jehol biota,which attracts many experts.In this paper the
文摘目的:探索血液中Ras转化酶1(Ras and a-factor converting enzyme 1,Rce1)蛋白在前列腺癌(prostate cancer,PCa)诊断中的意义。方法:收集2017年4月至2018年10月经病理确诊的共96例PCa患者和133例良性前列腺增生(benign prostatic hyperplasia,BPH)患者的血液标本,进行Western blot和酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测,30例泌尿结石、隐睾、精索静脉曲张患者作为对照组,分析评价血液中Rce1浓度值诊断PCa的效能。结果:3组间血液Rce1水平差异具有统计学意义。PCa组血液Rce1浓度值[(93.25±15.14)pg/μL]明显高于BPH组[(78.37±16.91)pg/μL]和对照组[(77.73±12.04)pg/μL](P=0.000);而BPH组和对照组间差异无统计学意义(P=0.843)。同时PCa患者血液Rce1浓度值高低与Gleason评分及N分期呈正相关(P=0.030,P=0.039)。血液Rce1截断值为74.98 pg/μL时最佳,其敏感性和特异性分别为89.7%、46.3%。血Rce1与前列腺特异抗原(prostate specific antigen,PSA)联合诊断曲线下面积(area under the curve,AUC)高于单独应用(P=0.000)。结论:血液Rce1蛋白检测有助于提高PCa的早期筛查率,联合应用PSA能够提高PCa的诊断效能,Rce1可能作为PCa筛查和判断预后的新标志物。
