诺如病毒是严重危害人类健康的重要食源性病原。作者应用3’RACE(rapid amplification of cDNA 3’ends)技术对一株诺如病毒分离株的基因组3’末端进行扩增,并对其核苷酸序列进行比较分析,从而可为该病的分子生物学检测提供阳性物质,并...诺如病毒是严重危害人类健康的重要食源性病原。作者应用3’RACE(rapid amplification of cDNA 3’ends)技术对一株诺如病毒分离株的基因组3’末端进行扩增,并对其核苷酸序列进行比较分析,从而可为该病的分子生物学检测提供阳性物质,并从分子水平上推测该病的来源。经过提取病毒总RNA、以3’RACE锚定引物进行反转录、用特异引物进行3’RACE,琼脂糖凝胶电泳检测结果表明成功扩增了诺如病毒基因组约3282 bp的基因片段,测序及序列BLAST分析证实该分离株属GⅡ-4型,与日本分离株同源。3’RACE扩增序列为诺如病毒的实验室检测及深入研究奠定了基础。展开更多
Total RNA was isolated from mycelium of T. harzianum by Total RNA extraction kit, and two clear bands of rRNA （28S and 18S） were observed in agarose electrophoresis. By joining the 3＇end sequence with the known SA7...Total RNA was isolated from mycelium of T. harzianum by Total RNA extraction kit, and two clear bands of rRNA （28S and 18S） were observed in agarose electrophoresis. By joining the 3＇end sequence with the known SA76 EST from cDNA library of T. harzianum, a full-length cDNA sequence of 2019bp was obtained, whose open reading frame contained 1593bp, a stop codon TAA, a 5＇untranslated region （5＇UTR） of 266bp, a 3＇untranslated region （3＇UTR） of 201bp, and poly （A） 29 encoded a protein of 530 amino acids, had a signal peptide. T. harzianum shared 53% identity of secreted aspartic proteinase gene with G. zeae, 37% with N. crassa and 36% with C. globosum. The full-length cDNA sequence of secreted aspartic proteinase gene from T. harzianum was cloned for the first time by using BD SMART RACE technique, which provides a foundation to obtain and validate functional genes of T. harzianum.展开更多
James Fenimore Cooper is a very famous and popular writer in America who is the founder of the America literature on romanticism, so he is called the first American novelist. In 1820, Cooper publishes his first novel,...James Fenimore Cooper is a very famous and popular writer in America who is the founder of the America literature on romanticism, so he is called the first American novelist. In 1820, Cooper publishes his first novel, from then on he publishes over 50 novels altogether. The characters we are talking about are from one of his best known novels"The Last of the Mohicans",the second part of The Leatherstocking Tales which is his most famous and influencing novel. Through the novel, the author believes that the process of the early American movement towards the west is actually a process of massacre of American Indians.The article points out that James Fenimore Cooper has sympathy with American Indians, but at the same time showing his ambiguities on many issues.展开更多
in order to improve the acquired level of heat acclimatization (HA) by training, on the basis of singlemode march training (SMT) or single-mode cross-country race training (SCT) , combined training method of march and...in order to improve the acquired level of heat acclimatization (HA) by training, on the basis of singlemode march training (SMT) or single-mode cross-country race training (SCT) , combined training method of march and cross-country race in the heat was adopted in this experiment ,and the effect of the training on heat tolerance was analyzed. Eight young males were asked to perform a simulated SMT and SCT for 90 min on a treadmill in a hot climate chamber (Td 32. 7℃ , WBGT 28. 4℃ ) once daily for 10 to 12 d within two weeks. The results showed that rectal temperature (Tr) was reduced by 0. 61℃ , heart rate (Hr) by 8. 5 b/min,sweat volume (SV)by 0. 220 kg, physiological strain index (PSI)by 0. 620,systolic pressure (SP)and diastolic pressure (DP) had a little change, pulse pressure (PP) and stroke volume (StV) increased , and cardiac output (CO) decreased a little at the middle stage and increased at the late stage due to Hr change. The standardized bicycle ergometer test (SBE test) in the heat (Td 37℃)has proved that these physiological indexes post-training had significant improvements ,as compared with those pre-training, suggesting that the combined training could raise heat-tolerance and was superior to the SMT in improving acquired heat acclimatization in same training duration and times , and to the SCT in regulating body temperature and cardiovascular function and in prolonging heat tolerance duration.展开更多
This paper reports the effects of two trainings-5 km cross-country race and 15 min heat-bath on theimprovement of heat-tolerance. All subjects of the training group were asked to perform a training once daily for 10to...This paper reports the effects of two trainings-5 km cross-country race and 15 min heat-bath on theimprovement of heat-tolerance. All subjects of the training group were asked to perform a training once daily for 10to 12 d within two weeks. The results proved that the cross-country race training both in the artificial hot chamberand on the drill ground of troop could obtain evident training-adaptation. The rectal temperature, heart rate, andsweat volume reduced by 0. 39℃, 13. 2 b/min and 338 g during training in field, respectively. In the same period ofcross-country race training, the normal military exercises, labours and natural heat acclimatization in the summercould only raise slightly the level of heat acclimatization in soldiers. Heat-bath training could reduce rectal temperature by 0. 6℃ during immersion in hot water, however, the level of heat-tolerance in a hot climatic chamber was inferior to that attained by cross-country race, march and running training.展开更多
《领先阅读·X计划》是北大附小开展'中国中小学生英语分级阅读体系标准研制'子课题实验所用的系列读物。读物围绕Cat、Tiger和他们的朋友拥有了可以把人变小的神奇手表而展开的一系列的故事,'The race'便是其中一...《领先阅读·X计划》是北大附小开展'中国中小学生英语分级阅读体系标准研制'子课题实验所用的系列读物。读物围绕Cat、Tiger和他们的朋友拥有了可以把人变小的神奇手表而展开的一系列的故事,'The race'便是其中一个。故事讲述了变小后的Tiger驾驶micro-buggy和骑着蜗牛的Cat竞速比赛。最终,'slow and steady'的Cat赢得了轻敌的Tiger。展开更多
基金This paper was supported by Development Program (863) of China (No. 2003AA241140)
文摘Total RNA was isolated from mycelium of T. harzianum by Total RNA extraction kit, and two clear bands of rRNA （28S and 18S） were observed in agarose electrophoresis. By joining the 3＇end sequence with the known SA76 EST from cDNA library of T. harzianum, a full-length cDNA sequence of 2019bp was obtained, whose open reading frame contained 1593bp, a stop codon TAA, a 5＇untranslated region （5＇UTR） of 266bp, a 3＇untranslated region （3＇UTR） of 201bp, and poly （A） 29 encoded a protein of 530 amino acids, had a signal peptide. T. harzianum shared 53% identity of secreted aspartic proteinase gene with G. zeae, 37% with N. crassa and 36% with C. globosum. The full-length cDNA sequence of secreted aspartic proteinase gene from T. harzianum was cloned for the first time by using BD SMART RACE technique, which provides a foundation to obtain and validate functional genes of T. harzianum.
文摘James Fenimore Cooper is a very famous and popular writer in America who is the founder of the America literature on romanticism, so he is called the first American novelist. In 1820, Cooper publishes his first novel, from then on he publishes over 50 novels altogether. The characters we are talking about are from one of his best known novels"The Last of the Mohicans",the second part of The Leatherstocking Tales which is his most famous and influencing novel. Through the novel, the author believes that the process of the early American movement towards the west is actually a process of massacre of American Indians.The article points out that James Fenimore Cooper has sympathy with American Indians, but at the same time showing his ambiguities on many issues.
文摘in order to improve the acquired level of heat acclimatization (HA) by training, on the basis of singlemode march training (SMT) or single-mode cross-country race training (SCT) , combined training method of march and cross-country race in the heat was adopted in this experiment ,and the effect of the training on heat tolerance was analyzed. Eight young males were asked to perform a simulated SMT and SCT for 90 min on a treadmill in a hot climate chamber (Td 32. 7℃ , WBGT 28. 4℃ ) once daily for 10 to 12 d within two weeks. The results showed that rectal temperature (Tr) was reduced by 0. 61℃ , heart rate (Hr) by 8. 5 b/min,sweat volume (SV)by 0. 220 kg, physiological strain index (PSI)by 0. 620,systolic pressure (SP)and diastolic pressure (DP) had a little change, pulse pressure (PP) and stroke volume (StV) increased , and cardiac output (CO) decreased a little at the middle stage and increased at the late stage due to Hr change. The standardized bicycle ergometer test (SBE test) in the heat (Td 37℃)has proved that these physiological indexes post-training had significant improvements ,as compared with those pre-training, suggesting that the combined training could raise heat-tolerance and was superior to the SMT in improving acquired heat acclimatization in same training duration and times , and to the SCT in regulating body temperature and cardiovascular function and in prolonging heat tolerance duration.
文摘This paper reports the effects of two trainings-5 km cross-country race and 15 min heat-bath on theimprovement of heat-tolerance. All subjects of the training group were asked to perform a training once daily for 10to 12 d within two weeks. The results proved that the cross-country race training both in the artificial hot chamberand on the drill ground of troop could obtain evident training-adaptation. The rectal temperature, heart rate, andsweat volume reduced by 0. 39℃, 13. 2 b/min and 338 g during training in field, respectively. In the same period ofcross-country race training, the normal military exercises, labours and natural heat acclimatization in the summercould only raise slightly the level of heat acclimatization in soldiers. Heat-bath training could reduce rectal temperature by 0. 6℃ during immersion in hot water, however, the level of heat-tolerance in a hot climatic chamber was inferior to that attained by cross-country race, march and running training.
文摘《领先阅读·X计划》是北大附小开展'中国中小学生英语分级阅读体系标准研制'子课题实验所用的系列读物。读物围绕Cat、Tiger和他们的朋友拥有了可以把人变小的神奇手表而展开的一系列的故事,'The race'便是其中一个。故事讲述了变小后的Tiger驾驶micro-buggy和骑着蜗牛的Cat竞速比赛。最终,'slow and steady'的Cat赢得了轻敌的Tiger。
