At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software too...At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software tools were used to analyze and predict the gene family and this gene.There were 30 members of the cucumber F-box gene family.The coding region of the cucumber CsFK111 gene was full-length 1314 bp,which encoded 437 amino acids and was predicted to be located in the nucleus.The protein encoded by this gene was a non-transmembrane protein,and the prediction of the secondary structure showed thatβ-lamellar structure and irregular crimp were dominant.A comparison of the phylogenetic tree showed that it was closest to cantaloupe and belonged to the same branch.The results provided a basis for future study on the regulation mechanism of the CsFK111 gene on cucumber dwarfing and also laid a foundation for further study of FBK family proteins.展开更多
The sea cucumber is a highly esteemed source of nourishment,recognized as one of the“Eight Treasures of the Sea.”The bioactive compounds derived from sea cucumbers exhibit diverse physiological activities,including ...The sea cucumber is a highly esteemed source of nourishment,recognized as one of the“Eight Treasures of the Sea.”The bioactive compounds derived from sea cucumbers exhibit diverse physiological activities,including anti-tumor,antioxidant,anti-coagulation,anti-viral,anti-fatigue,immune enhancement,cognitive improvement,and metabolic regulation.Notably,within the skincare sector,these compounds demonstrate significant anti-aging,moisturizing,whitening,wrinkle reduction,repair and inhibition of melanin production properties.This article assesses the current state of sea cucumber aquaculture in China and the utilization of its bioactive ingredients in skincare formulations.The objective is to furnish additional raw materials and semi-finished products for China’s skincare and pharmaceutical industries,to advance the integration of sea cucumber bioactive components within these sectors,and to invigorate the rapid development of the tropical sea cucumber breeding industry.展开更多
To assess the risks of forchlorfenuron after application, a residue analysis method for ferchlorfenuron in cucumbers and the red soil of Southern China was established, and the dissipation behavior and residue charact...To assess the risks of forchlorfenuron after application, a residue analysis method for ferchlorfenuron in cucumbers and the red soil of Southern China was established, and the dissipation behavior and residue characteristics of forchlorfenuron were studied under field conditions. The field trials, including dissipation and residue experiments, were conducted in Hunan, Yunnan and Hainan Provinces. Forchlorfenuron was applied at 32 a.i.g ha^-1 rate for the dissipation study and both 21 and 32 a.i.g ha^-1 for the residue study. Representative cucumbers and soil samples were collected at predetermined intervals for HPLC for UV- detector analysis. The average recoveries of the method were 80.8%-92.0% with coefficients variation （CV） between 0.4% and 11.8%. The detection limit of forchlorfenuron in cucumbers and soil samples was 0.003 mg·kg^-1 and the minimum detectable amount was 3.0× 10^-10 g. The half life of forchlorfenuron in cucumbers in the three provinces changed as： Yunnan （5.50 days）, Hunan （5.88 days）, and Hainan （6.53 days） and in the soil was observed： Hainan （6.54 days）, Hunan （7.64 days）, and Yunnan （8.39 days）. The maximum terminal residue in cucumbers at 32 a.i.g ha^-1 rate after 5 days was 0.009 mg·kg^-1 （Yunnan） followed by 0.0085 mg·kg^-1 （Hainan） and 0.0082 mg·kg^-1（Hunan） which was below MRL value of 0.01 mg·kg^-1 established by the USA, EPA. So a waiting period of 5 days should be established after spaying on cucumbers to avoid hazard to human beings.展开更多
Dwarfism is one of the most important traits in crop breeding. In this study, a dwarf cucumber D0462 and a vine NA129 were taken as experimental materials. The metabolism of hydrogen peroxide （H2O2） and superoxide a...Dwarfism is one of the most important traits in crop breeding. In this study, a dwarf cucumber D0462 and a vine NA129 were taken as experimental materials. The metabolism of hydrogen peroxide （H2O2） and superoxide anion radical （O2-·, activities of antioxidant enzymes, and the levels of endogenous hormones of the two cultivars were compared. In the whole growth period, D0462 maintained higher H2O2 and O2-· levels than NA129. The activities of SOD （superoxide dismutase）, CAT （catalase）, APX （ascorbate peroxidase） and IAAO （indoleacetic acid oxidase） in D0462 were found higher than those in NA129. The contents of gibberellic acid （GA3）, indole-3-acetic acid （IAA）, zeatin riboside （ZR） and jasmonic acid （JA） in intemode were determined by enzyme-linked immunosorbent assay （ELISA）. The intemode ofD0462 remained lower levels of GA3, IAA, and ZR. In contrast, JA level in internode of D0462 was higher than that of NA129. Compared with NA129, the cell mumbers in intemode of D0462 did not change, but cell size evidently decreased. The chloroplasts in mature mesophyll D0462 exhibited swollen filled with starch grains instead of regular oval ones, and thylakoids of chloroplast swelled and became ambiguous.展开更多
基金Supported by the National Natural Science Foundation of China(32272724)the National Science Foundation of Heilongjiang Province,China(LH2019C033)。
文摘At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software tools were used to analyze and predict the gene family and this gene.There were 30 members of the cucumber F-box gene family.The coding region of the cucumber CsFK111 gene was full-length 1314 bp,which encoded 437 amino acids and was predicted to be located in the nucleus.The protein encoded by this gene was a non-transmembrane protein,and the prediction of the secondary structure showed thatβ-lamellar structure and irregular crimp were dominant.A comparison of the phylogenetic tree showed that it was closest to cantaloupe and belonged to the same branch.The results provided a basis for future study on the regulation mechanism of the CsFK111 gene on cucumber dwarfing and also laid a foundation for further study of FBK family proteins.
文摘The sea cucumber is a highly esteemed source of nourishment,recognized as one of the“Eight Treasures of the Sea.”The bioactive compounds derived from sea cucumbers exhibit diverse physiological activities,including anti-tumor,antioxidant,anti-coagulation,anti-viral,anti-fatigue,immune enhancement,cognitive improvement,and metabolic regulation.Notably,within the skincare sector,these compounds demonstrate significant anti-aging,moisturizing,whitening,wrinkle reduction,repair and inhibition of melanin production properties.This article assesses the current state of sea cucumber aquaculture in China and the utilization of its bioactive ingredients in skincare formulations.The objective is to furnish additional raw materials and semi-finished products for China’s skincare and pharmaceutical industries,to advance the integration of sea cucumber bioactive components within these sectors,and to invigorate the rapid development of the tropical sea cucumber breeding industry.
文摘To assess the risks of forchlorfenuron after application, a residue analysis method for ferchlorfenuron in cucumbers and the red soil of Southern China was established, and the dissipation behavior and residue characteristics of forchlorfenuron were studied under field conditions. The field trials, including dissipation and residue experiments, were conducted in Hunan, Yunnan and Hainan Provinces. Forchlorfenuron was applied at 32 a.i.g ha^-1 rate for the dissipation study and both 21 and 32 a.i.g ha^-1 for the residue study. Representative cucumbers and soil samples were collected at predetermined intervals for HPLC for UV- detector analysis. The average recoveries of the method were 80.8%-92.0% with coefficients variation （CV） between 0.4% and 11.8%. The detection limit of forchlorfenuron in cucumbers and soil samples was 0.003 mg·kg^-1 and the minimum detectable amount was 3.0× 10^-10 g. The half life of forchlorfenuron in cucumbers in the three provinces changed as： Yunnan （5.50 days）, Hunan （5.88 days）, and Hainan （6.53 days） and in the soil was observed： Hainan （6.54 days）, Hunan （7.64 days）, and Yunnan （8.39 days）. The maximum terminal residue in cucumbers at 32 a.i.g ha^-1 rate after 5 days was 0.009 mg·kg^-1 （Yunnan） followed by 0.0085 mg·kg^-1 （Hainan） and 0.0082 mg·kg^-1（Hunan） which was below MRL value of 0.01 mg·kg^-1 established by the USA, EPA. So a waiting period of 5 days should be established after spaying on cucumbers to avoid hazard to human beings.
基金Supported by the China Postdoctoral Science Foundation(2012M520701)the Key Laboratory Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture(CVB2012-001)
文摘Dwarfism is one of the most important traits in crop breeding. In this study, a dwarf cucumber D0462 and a vine NA129 were taken as experimental materials. The metabolism of hydrogen peroxide （H2O2） and superoxide anion radical （O2-·, activities of antioxidant enzymes, and the levels of endogenous hormones of the two cultivars were compared. In the whole growth period, D0462 maintained higher H2O2 and O2-· levels than NA129. The activities of SOD （superoxide dismutase）, CAT （catalase）, APX （ascorbate peroxidase） and IAAO （indoleacetic acid oxidase） in D0462 were found higher than those in NA129. The contents of gibberellic acid （GA3）, indole-3-acetic acid （IAA）, zeatin riboside （ZR） and jasmonic acid （JA） in intemode were determined by enzyme-linked immunosorbent assay （ELISA）. The intemode ofD0462 remained lower levels of GA3, IAA, and ZR. In contrast, JA level in internode of D0462 was higher than that of NA129. Compared with NA129, the cell mumbers in intemode of D0462 did not change, but cell size evidently decreased. The chloroplasts in mature mesophyll D0462 exhibited swollen filled with starch grains instead of regular oval ones, and thylakoids of chloroplast swelled and became ambiguous.
