OBJECTIVE Prostate cancer is one of the most commonly diagnosed cancers worldwide.Current therapies for metastatic prostate cancer are only marginally effective,and hence novel treatment modalities are urgently needed...OBJECTIVE Prostate cancer is one of the most commonly diagnosed cancers worldwide.Current therapies for metastatic prostate cancer are only marginally effective,and hence novel treatment modalities are urgently needed.Considerable evidence suggests that chronic inflammation plays a pivotal role in the development and progression of prostate cancer.Thus agents that can suppress these inflammatory mediators could form the basis of novel therapy for prostate cancer patients.In our study,we focused on analyzing the potential anticancer effects of nimbolide,a terpenoid lactone derived from Azadirachta indica(Neem tree)against prostate cancer.METHODS Molecular biology techniques such as western blot analysis,DNA binding,luciferase assays,and immunohistochemistry were used for both in vitro and in vivo experiments.RESULTS Data from the in vitrostudies indicated that nimbolide could inhibit cell proliferation,induce apoptosis and suppress cellular invasion and migration.Interestingly,nimbolide also abrogated the activation of pro-inflammatory STAT 3 transcription factor,and this effect was found to be mediated via an increased production of reactive oxygen species(ROS),whereas depletion of ROS attenuated pSTAT 3 inhibitory effects of the drug.The in vivo efficacy of nimbolide was also noted in transgenic adenocarcinoma of mouse prostate(TRAMP)model,in which this triterpenoid significantly suppressed the tumor progression and growth without exhibiting any substantial adverse effects.CONCLUSION Overall our findings indicate that nimbolide exhibits significant anticancer effects in prostate cancer,and these effects may be mediated at least in part through the modulation of STAT 3 signaling pathway.展开更多
经尿道前列腺电切术（transurethral resection of the prostate，TURP）因损伤小、恢复快、疗效好，目前己广泛应用于前列腺增生的临床治疗。但是由于术前尿路严重感染、手术创伤出血、术后留置导尿管等刺激，出现膀胱痉挛疼痛，增加...经尿道前列腺电切术（transurethral resection of the prostate，TURP）因损伤小、恢复快、疗效好，目前己广泛应用于前列腺增生的临床治疗。但是由于术前尿路严重感染、手术创伤出血、术后留置导尿管等刺激，出现膀胱痉挛疼痛，增加围术期风险，故完善术后镇痛十分重要。展开更多
前列腺特异性抗原(prostate specific antigen,PSA)是目前列腺癌早期诊断的重要指标,但PSA是组织特异性而非肿瘤特异性,一些前列腺良性疾病如前列腺增生、前列腺炎亦可导致PSA升高。本文回顾分析了2013年1月-2017年12月在我院泌尿外科...前列腺特异性抗原(prostate specific antigen,PSA)是目前列腺癌早期诊断的重要指标,但PSA是组织特异性而非肿瘤特异性,一些前列腺良性疾病如前列腺增生、前列腺炎亦可导致PSA升高。本文回顾分析了2013年1月-2017年12月在我院泌尿外科门诊或住院部就诊的833例经超声引导下前列腺穿刺活检病理确诊为前列腺增生或前列腺癌患者的PSA值,现分析总结如下。1资料与方法1.1一般资料回顾性收集2013年1月-2017年12月在我院泌尿外科门诊就医和住院的疑似前列腺癌而行前列腺穿刺活检的患者共833例;PSA异常(>4 ng/mL)者811例;PSA正常(0~4 ng/mL)者22例:其中直肠指检(DRE)异常者12例,MRI发现异常病灶者7例,经直肠超声检查(TRUS)异常者3例。患者年龄25.0~90.0岁,平均(68.5±8.4)岁。展开更多
Estrogens are critical for bone health and the development of breast and prostate tissues.At menopause,our cohort studies show that naturally occurring estrogens in the body protect against osteoporotic hip fractures,...Estrogens are critical for bone health and the development of breast and prostate tissues.At menopause,our cohort studies show that naturally occurring estrogens in the body protect against osteoporotic hip fractures,but can also increase the risk of breast cancer.Extracts of Epimediumspp.is widely used to treat osteoporosis and improve general health in post-menopausal women.Prenylated flavonoids from Epimedium spp.exert potent estrogenic effects making it an attractive alternative to estrogen replacement therapy for treatment of osteoporosis.Lead compounds from epimedium such as icaritin impact pro-osteoblastic,anti-osteoclastic signaling pathways in bone cells;and anti-tumour effect on nuclear receptors in breast and prostate cancer animal models.Oral administration of a Epimedium spp.extract in openlabel,two-period,randomized,crossover study indicate significant ex-vivo effects on ERαand ERβ-mediated bioactivities and breast cancer cell proliferation.Challenges in the taxonomy,standardization and quality manufacturing of extracts suitable for pharmacokinetic,pharmacodynamics and efficacy studies in humans will be discussed.展开更多
由前列腺癌基金会（ Prostate Cancer Foundation, PCF ） 、中国前列腺癌联盟（ Chinese Prostate Cancer Consortium，CPCC）和第二军医大学长海医院共同主办的第二届前列腺癌基金会中国科学研讨会于2013年5月18日在上海成功召开，近...由前列腺癌基金会（ Prostate Cancer Foundation, PCF ） 、中国前列腺癌联盟（ Chinese Prostate Cancer Consortium，CPCC）和第二军医大学长海医院共同主办的第二届前列腺癌基金会中国科学研讨会于2013年5月18日在上海成功召开，近百名国内外泌尿外科专家学者参会。展开更多
基金The project supported by agrant from NUS Academic Research Fund
文摘OBJECTIVE Prostate cancer is one of the most commonly diagnosed cancers worldwide.Current therapies for metastatic prostate cancer are only marginally effective,and hence novel treatment modalities are urgently needed.Considerable evidence suggests that chronic inflammation plays a pivotal role in the development and progression of prostate cancer.Thus agents that can suppress these inflammatory mediators could form the basis of novel therapy for prostate cancer patients.In our study,we focused on analyzing the potential anticancer effects of nimbolide,a terpenoid lactone derived from Azadirachta indica(Neem tree)against prostate cancer.METHODS Molecular biology techniques such as western blot analysis,DNA binding,luciferase assays,and immunohistochemistry were used for both in vitro and in vivo experiments.RESULTS Data from the in vitrostudies indicated that nimbolide could inhibit cell proliferation,induce apoptosis and suppress cellular invasion and migration.Interestingly,nimbolide also abrogated the activation of pro-inflammatory STAT 3 transcription factor,and this effect was found to be mediated via an increased production of reactive oxygen species(ROS),whereas depletion of ROS attenuated pSTAT 3 inhibitory effects of the drug.The in vivo efficacy of nimbolide was also noted in transgenic adenocarcinoma of mouse prostate(TRAMP)model,in which this triterpenoid significantly suppressed the tumor progression and growth without exhibiting any substantial adverse effects.CONCLUSION Overall our findings indicate that nimbolide exhibits significant anticancer effects in prostate cancer,and these effects may be mediated at least in part through the modulation of STAT 3 signaling pathway.
文摘前列腺特异性抗原(prostate specific antigen,PSA)是目前列腺癌早期诊断的重要指标,但PSA是组织特异性而非肿瘤特异性,一些前列腺良性疾病如前列腺增生、前列腺炎亦可导致PSA升高。本文回顾分析了2013年1月-2017年12月在我院泌尿外科门诊或住院部就诊的833例经超声引导下前列腺穿刺活检病理确诊为前列腺增生或前列腺癌患者的PSA值,现分析总结如下。1资料与方法1.1一般资料回顾性收集2013年1月-2017年12月在我院泌尿外科门诊就医和住院的疑似前列腺癌而行前列腺穿刺活检的患者共833例;PSA异常(>4 ng/mL)者811例;PSA正常(0~4 ng/mL)者22例:其中直肠指检(DRE)异常者12例,MRI发现异常病灶者7例,经直肠超声检查(TRUS)异常者3例。患者年龄25.0~90.0岁,平均(68.5±8.4)岁。
文摘Estrogens are critical for bone health and the development of breast and prostate tissues.At menopause,our cohort studies show that naturally occurring estrogens in the body protect against osteoporotic hip fractures,but can also increase the risk of breast cancer.Extracts of Epimediumspp.is widely used to treat osteoporosis and improve general health in post-menopausal women.Prenylated flavonoids from Epimedium spp.exert potent estrogenic effects making it an attractive alternative to estrogen replacement therapy for treatment of osteoporosis.Lead compounds from epimedium such as icaritin impact pro-osteoblastic,anti-osteoclastic signaling pathways in bone cells;and anti-tumour effect on nuclear receptors in breast and prostate cancer animal models.Oral administration of a Epimedium spp.extract in openlabel,two-period,randomized,crossover study indicate significant ex-vivo effects on ERαand ERβ-mediated bioactivities and breast cancer cell proliferation.Challenges in the taxonomy,standardization and quality manufacturing of extracts suitable for pharmacokinetic,pharmacodynamics and efficacy studies in humans will be discussed.
文摘由前列腺癌基金会（ Prostate Cancer Foundation, PCF ） 、中国前列腺癌联盟（ Chinese Prostate Cancer Consortium，CPCC）和第二军医大学长海医院共同主办的第二届前列腺癌基金会中国科学研讨会于2013年5月18日在上海成功召开，近百名国内外泌尿外科专家学者参会。
