Proanthocyanidins(PAs)are the most broadly distributed secondary metabolites that play important roles in various aspects of plant development and response to biotic and abiotic stresses.In this study,we cloned a R2R3...Proanthocyanidins(PAs)are the most broadly distributed secondary metabolites that play important roles in various aspects of plant development and response to biotic and abiotic stresses.In this study,we cloned a R2R3 MYB gene LoMYB29,which has a full-length coding sequence of 921 bp identified in Larix olgensis.Quantitative real-time reverse transcription polymerase chain reaction analysis indicates that LoMYB29 is expressed under mechanical wounding,high light intensity,and NaCl,PEG6000,Methyl Jasmonate,and abscisic acid treatments.Subcellular localization analysis and yeast twohybrid assay localized LoMYB29 to the nucleus,acting as a transcriptional activator.Staining with 4-dimethylaminocinnamaldehyde showed a darker blue-purple color in LoMYB29-overexpressing Arabidopsis seeds compared to that of wild seeds.LoMYB29-overexpression resulted in a significant increase in leaf PA content.The expression of early flavonoid biosynthesis-related gene CHI and late flavonoid biosynthesis-related genes,including DFR,LDOX,and ANR(PA branch gene),were also activated in transgenic plants overexpressing LoMYB29.The results indicate that LoMYB29 plays a positive role in the regulation of PA biosynthesis by activating the expression of PA biosynthetic genes.展开更多
Background Atherosclerotic plaques indicate the occurrence of ischemia events and it is a difficult task for clinical physicians. Grape seed proanthocyanidin extract （GSPE） has been reported to exert an antiatheroge...Background Atherosclerotic plaques indicate the occurrence of ischemia events and it is a difficult task for clinical physicians. Grape seed proanthocyanidin extract （GSPE） has been reported to exert an antiatherogenic effect by inducing regression of atherosclerotic plaques in animal experimental studies. In this study, the antiatherogenic effect of GSPE has been investigated in clinical use. Methods Consecu- tive 287 patients diagnosed with asymptomatic carotid plaques or abnormal plaque free carotid intima-media thickness （CIMT） were ran- domly assigned to the GSPE group （n = 146） or control group （n = 141）. The patients in the GSPE group received GSPE 200 mg per day orally, while patients in the control group were only enrolled in a lifestyle intervention program. Carotid ultrasound examination was per- formed at baseline and 6, 12, 24 months during follow-up. Mean maximum CIMT （MMCIMT）, plaque score, echogenicity of plaques and ischemic vascular events were recorded. Results As anticipated, after treatment, GSPE resulted in significant reduction in MMCIMT pro- gression （4.2% decrease after six months, 4.9% decrease after 12 months and 5.8% decrease after 24 months） and plaque score （10.9% de- crease after six months, 24.1% decrease after 12 months and 33.1% decrease after 24 months） for the primary outcome, while MMCIMT and plaque score were stable and even increased with the time going on in control group. The number of plaques and unstable plaques also de- creased after treatment of GSPE. Furthermore, the carotid plaque can disappear after treatment with GSPE. The incidence rate for transitory ischemic attack （TIA）, arterial revascularization procedure, and hospital readmission for unstable angina in GSPE group were statistically significant lower （P = 0.02, 0.08, 0.002, respectively） compared with the control group. Conclusions GSPE inhibited the progression of MMCIMT and reduced carotid plaque size in GSPE treated patients, and with extended treatment, the superior efficacy on MMCIMT and carotid plaque occurred. Furthermore, the GSPE group showed lower rates of clinical vascular events.展开更多
利用植物多酚原花青素(oligomeric proanthocyanidins,OPC)与大豆分离蛋白(soybean protein isolate,SPI)的相互作用,采用葡萄糖酸内酯诱导结合超声波处理制备OPC-SPI凝胶,旨在改善SPI凝胶性能。研究不同浓度OPC(0.1%、0.3%、0.5%)对SP...利用植物多酚原花青素(oligomeric proanthocyanidins,OPC)与大豆分离蛋白(soybean protein isolate,SPI)的相互作用,采用葡萄糖酸内酯诱导结合超声波处理制备OPC-SPI凝胶,旨在改善SPI凝胶性能。研究不同浓度OPC(0.1%、0.3%、0.5%)对SPI凝胶流变特性和抗氧化活性的影响。结果表明,随着OPC浓度的增加,包埋率增加,荷载量减小,最大荷载量达到35.5753μg/mg。随着OPC浓度的增加,OPC-SPI凝胶的弹性模量和黏性模量增加,损耗因子随之降低,对频率的依赖性降低;但对凝胶的黏度无显著差异。OPC-SPI对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基以及羟自由基的清除能力优于SPI。OPC能够有效增强SPI的凝胶黏弹性和抗氧化活性,为进一步开发新型抗氧化复合SPI凝胶提供了理论依据。展开更多
基金supported by the Genetically Modified Organisms Breeding Major Projects of China(2018ZX08022001)111 Project(B16010)National High Technology Research and Development Program of China(863 Program,2013AA102704)
文摘Proanthocyanidins(PAs)are the most broadly distributed secondary metabolites that play important roles in various aspects of plant development and response to biotic and abiotic stresses.In this study,we cloned a R2R3 MYB gene LoMYB29,which has a full-length coding sequence of 921 bp identified in Larix olgensis.Quantitative real-time reverse transcription polymerase chain reaction analysis indicates that LoMYB29 is expressed under mechanical wounding,high light intensity,and NaCl,PEG6000,Methyl Jasmonate,and abscisic acid treatments.Subcellular localization analysis and yeast twohybrid assay localized LoMYB29 to the nucleus,acting as a transcriptional activator.Staining with 4-dimethylaminocinnamaldehyde showed a darker blue-purple color in LoMYB29-overexpressing Arabidopsis seeds compared to that of wild seeds.LoMYB29-overexpression resulted in a significant increase in leaf PA content.The expression of early flavonoid biosynthesis-related gene CHI and late flavonoid biosynthesis-related genes,including DFR,LDOX,and ANR(PA branch gene),were also activated in transgenic plants overexpressing LoMYB29.The results indicate that LoMYB29 plays a positive role in the regulation of PA biosynthesis by activating the expression of PA biosynthetic genes.
文摘Background Atherosclerotic plaques indicate the occurrence of ischemia events and it is a difficult task for clinical physicians. Grape seed proanthocyanidin extract （GSPE） has been reported to exert an antiatherogenic effect by inducing regression of atherosclerotic plaques in animal experimental studies. In this study, the antiatherogenic effect of GSPE has been investigated in clinical use. Methods Consecu- tive 287 patients diagnosed with asymptomatic carotid plaques or abnormal plaque free carotid intima-media thickness （CIMT） were ran- domly assigned to the GSPE group （n = 146） or control group （n = 141）. The patients in the GSPE group received GSPE 200 mg per day orally, while patients in the control group were only enrolled in a lifestyle intervention program. Carotid ultrasound examination was per- formed at baseline and 6, 12, 24 months during follow-up. Mean maximum CIMT （MMCIMT）, plaque score, echogenicity of plaques and ischemic vascular events were recorded. Results As anticipated, after treatment, GSPE resulted in significant reduction in MMCIMT pro- gression （4.2% decrease after six months, 4.9% decrease after 12 months and 5.8% decrease after 24 months） and plaque score （10.9% de- crease after six months, 24.1% decrease after 12 months and 33.1% decrease after 24 months） for the primary outcome, while MMCIMT and plaque score were stable and even increased with the time going on in control group. The number of plaques and unstable plaques also de- creased after treatment of GSPE. Furthermore, the carotid plaque can disappear after treatment with GSPE. The incidence rate for transitory ischemic attack （TIA）, arterial revascularization procedure, and hospital readmission for unstable angina in GSPE group were statistically significant lower （P = 0.02, 0.08, 0.002, respectively） compared with the control group. Conclusions GSPE inhibited the progression of MMCIMT and reduced carotid plaque size in GSPE treated patients, and with extended treatment, the superior efficacy on MMCIMT and carotid plaque occurred. Furthermore, the GSPE group showed lower rates of clinical vascular events.
文摘利用植物多酚原花青素(oligomeric proanthocyanidins,OPC)与大豆分离蛋白(soybean protein isolate,SPI)的相互作用,采用葡萄糖酸内酯诱导结合超声波处理制备OPC-SPI凝胶,旨在改善SPI凝胶性能。研究不同浓度OPC(0.1%、0.3%、0.5%)对SPI凝胶流变特性和抗氧化活性的影响。结果表明,随着OPC浓度的增加,包埋率增加,荷载量减小,最大荷载量达到35.5753μg/mg。随着OPC浓度的增加,OPC-SPI凝胶的弹性模量和黏性模量增加,损耗因子随之降低,对频率的依赖性降低;但对凝胶的黏度无显著差异。OPC-SPI对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基以及羟自由基的清除能力优于SPI。OPC能够有效增强SPI的凝胶黏弹性和抗氧化活性,为进一步开发新型抗氧化复合SPI凝胶提供了理论依据。
