Background Traffic accidents,anti-terrorism,gas and chemical dangerous goods explosions,earthquake shock wave damage,and falling impacts in daily life and other events involving impact loads cause great harm to human ...Background Traffic accidents,anti-terrorism,gas and chemical dangerous goods explosions,earthquake shock wave damage,and falling impacts in daily life and other events involving impact loads cause great harm to human organs and tissues,and even life-threatening.Such injuries are called impact damage.Although during the previous wars,the treatment of impact injuries has been greatly improved,and its treatment has been widely used in clinical practice.However,under the current development of society,the impact damage incident has not only been limited to the battlefield.Extreme organizations,frequent industrial production accidents,aircraft trains and other accidents have extended the impact damage incidents into daily society,seriously jeopardizing the health of civilians.Therefore,in order to better treat the injured organs under the impact load,such as the reconstruction and recovery of organ tissues,it is necessary to establish a corresponding system of clinical treatment methods for impact damage.In vitro models of traumatic injury are helping elucidate the pathobiological mechanisms responsible for dysfunction and delayed cell functional variation after mechanical stimulation of the single waveform pressure.It is likely that injury outcome is related to the biomechanical parameters of the traumatic event such as amplitudes and durations.However,the influence of impulsive pressure on endothelial function has not yet been fully studied in vitro.In this study,we developed a pressure loading device that produced positive by modifying an in vitro fluid percussion model and examined the effects of the pressures on macrophages’basic functions.Methods To model variations in the biomechanical injury parameters and simplify the experiment,single-use syringe was chosen to be the cell container and a drop hammer driven fluid percussion injury system(FPI)was designed and built to generate a single waveform with adjustable peak pressure and durations.Mice macrophage cells(Raw 264.7)were subjected to three types of the single positive pressure(120 kPa,550 kPa and 1 100 kPa).Every 12 hours we detected its basic functions(including phagocytosis and proliferative capacity)during the following 48 hours,also the immediate cell death.Results This single waveform pressure loading device could produce positive pressure with amplitudes of 70~1 200 kPa.After the pressure loading,there is no significant differences between the control cells and experiment cells.However,it does have a notable effect on its basic functions.The results showed that its phagocytosis and proliferative capacity were getting increased with a peak value on36 h and suddenly decreasing on 48 h.Moreover,these 4 regular curves are in proportion to the pressure.And the experimental results also indicate that the cell impact platform can achieve a single impact loading on the cells.The impact mainly causes the functional changes of RAW264.7 cells instead of directly causing its death.The cell proliferation activity and phagocytosis function are enhanced to some extent.Conclusions Those results indicate that single waveform pressure have a main effect on cell’s biological functions,not on cell death.And these effects on functions did have a regular functional rela-tionship.To explore more regular curves and the mechanism,we need more experiments such as genomics technical.展开更多
As a newly deVeloped method,high temperature in situ observation method can be used to observe directly the interface changes and study the kinetics mechanism during crystal growth.By our newly designed high temperatu...As a newly deVeloped method,high temperature in situ observation method can be used to observe directly the interface changes and study the kinetics mechanism during crystal growth.By our newly designed high temperature in situ observation equiPment,the interface changes of Bi_(12)SiO_(20) crystal growth from melt were studied.展开更多
Fromthe enterprise,individual and government,which are the three main economic behavior subject,this paper analysis factors impact of flow behavior main body and the change of the objective function,constraint functio...Fromthe enterprise,individual and government,which are the three main economic behavior subject,this paper analysis factors impact of flow behavior main body and the change of the objective function,constraint function and the impact of the change of behavior equation,and then summarizes their effects on the integration of urban and rural development,to build fromexogenous shocks to the endogenous to adapt to the economic decision-making framework,clarify elements flow affect the running mechanismof the urban-rural integration.展开更多
本研究采用不同糊化度(degrees of gelatinization,DSG)的马铃薯淀粉基与玉米油制备油相质量分数为30%的乳液,并通过贮藏稳定性、傅里叶变换红外光谱、Turbiscan稳定性指数(Turbiscan stability index,TSI)、粒径、微观结构和流变特性...本研究采用不同糊化度(degrees of gelatinization,DSG)的马铃薯淀粉基与玉米油制备油相质量分数为30%的乳液,并通过贮藏稳定性、傅里叶变换红外光谱、Turbiscan稳定性指数(Turbiscan stability index,TSI)、粒径、微观结构和流变特性评估淀粉DSG对乳液稳定性的影响。结果发现,乳液稳定性随着淀粉DSG的增加呈现出先增后减的趋势。当淀粉DSG≤67.03%时,随着淀粉DSG的增加,乳液红外光谱中水和淀粉分子特征峰越来越明显,乳液的粒径和TSI减小。这归因于糊化后淀粉分子的疏水性增加和浸出更多的直链淀粉分子,使得更多的淀粉颗粒参与形成乳液,增加了颗粒在液滴表面的覆盖率,从而形成更小的液滴和更稳定的乳液。当淀粉DSG为67.03%时乳液表现出最小的油滴粒径和TSI,其稳定性最好。当淀粉进一步糊化(DSG≥71.81%),更多的直链淀粉浸出,彼此间发生混乱缠结作用,阻碍油相融入淀粉基中,乳液粒径增大,稳定性降低。此外,DSG≥64.14%的淀粉基乳液贮藏21 d不发生分层现象;乳液的激光扫描共聚焦显微镜观察和流变学分析结果表明,乳液凝胶结构的强弱与油滴间相互作用密切相关,油滴间排列越致密,乳液凝胶表观黏度和储存模量越高,凝胶网络结构越强。此外,糊化后淀粉分子Zeta电位绝对值的降低有利于颗粒间相互靠近,也可能提高了连续相中颗粒的网络结构强度,从而提高了乳液的稳定性。本研究结果有助于进一步了解淀粉糊化稳定乳液的机理,对利用淀粉基开发更稳定的乳液具有参考作用。展开更多
基金financially supported by the National Natural Science Foundation of China ( 11722220, 11672246,11472224)
文摘Background Traffic accidents,anti-terrorism,gas and chemical dangerous goods explosions,earthquake shock wave damage,and falling impacts in daily life and other events involving impact loads cause great harm to human organs and tissues,and even life-threatening.Such injuries are called impact damage.Although during the previous wars,the treatment of impact injuries has been greatly improved,and its treatment has been widely used in clinical practice.However,under the current development of society,the impact damage incident has not only been limited to the battlefield.Extreme organizations,frequent industrial production accidents,aircraft trains and other accidents have extended the impact damage incidents into daily society,seriously jeopardizing the health of civilians.Therefore,in order to better treat the injured organs under the impact load,such as the reconstruction and recovery of organ tissues,it is necessary to establish a corresponding system of clinical treatment methods for impact damage.In vitro models of traumatic injury are helping elucidate the pathobiological mechanisms responsible for dysfunction and delayed cell functional variation after mechanical stimulation of the single waveform pressure.It is likely that injury outcome is related to the biomechanical parameters of the traumatic event such as amplitudes and durations.However,the influence of impulsive pressure on endothelial function has not yet been fully studied in vitro.In this study,we developed a pressure loading device that produced positive by modifying an in vitro fluid percussion model and examined the effects of the pressures on macrophages’basic functions.Methods To model variations in the biomechanical injury parameters and simplify the experiment,single-use syringe was chosen to be the cell container and a drop hammer driven fluid percussion injury system(FPI)was designed and built to generate a single waveform with adjustable peak pressure and durations.Mice macrophage cells(Raw 264.7)were subjected to three types of the single positive pressure(120 kPa,550 kPa and 1 100 kPa).Every 12 hours we detected its basic functions(including phagocytosis and proliferative capacity)during the following 48 hours,also the immediate cell death.Results This single waveform pressure loading device could produce positive pressure with amplitudes of 70~1 200 kPa.After the pressure loading,there is no significant differences between the control cells and experiment cells.However,it does have a notable effect on its basic functions.The results showed that its phagocytosis and proliferative capacity were getting increased with a peak value on36 h and suddenly decreasing on 48 h.Moreover,these 4 regular curves are in proportion to the pressure.And the experimental results also indicate that the cell impact platform can achieve a single impact loading on the cells.The impact mainly causes the functional changes of RAW264.7 cells instead of directly causing its death.The cell proliferation activity and phagocytosis function are enhanced to some extent.Conclusions Those results indicate that single waveform pressure have a main effect on cell’s biological functions,not on cell death.And these effects on functions did have a regular functional rela-tionship.To explore more regular curves and the mechanism,we need more experiments such as genomics technical.
文摘As a newly deVeloped method,high temperature in situ observation method can be used to observe directly the interface changes and study the kinetics mechanism during crystal growth.By our newly designed high temperature in situ observation equiPment,the interface changes of Bi_(12)SiO_(20) crystal growth from melt were studied.
文摘Fromthe enterprise,individual and government,which are the three main economic behavior subject,this paper analysis factors impact of flow behavior main body and the change of the objective function,constraint function and the impact of the change of behavior equation,and then summarizes their effects on the integration of urban and rural development,to build fromexogenous shocks to the endogenous to adapt to the economic decision-making framework,clarify elements flow affect the running mechanismof the urban-rural integration.
