Proteomic approaches using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were adopted to identify proteins in poplar leaves that were differentially expressed in response to black spot disease ...Proteomic approaches using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were adopted to identify proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen: Marssonina brunnea f. sp. multigermtubi. Proteins were extracted from leaves at 0, 12, 24, 48 and 72 hrs after pathogen inoculation. Five hundred reproducible expressed protein spots were detected. Forty-one differentially expressed protein spots resolved on the 2-DE gels were subjected to matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. The identified proteins were mainly related to photosynthesis, metabolism and defense response. Finally, proteins involved in plant defense mechanism were discussed.展开更多
探究滴灌水肥耦合对‘新林1号’杨(Populus cathayana×canadansis‘Xinlin1’)生长和光合生理特性的影响,旨在选出促进‘新林1号’杨生长的最佳水肥耦合措施。以10年生‘新林1号’杨为研究对象,设置-20 k Pa(I_(20))、-33 k Pa(I_(...探究滴灌水肥耦合对‘新林1号’杨(Populus cathayana×canadansis‘Xinlin1’)生长和光合生理特性的影响,旨在选出促进‘新林1号’杨生长的最佳水肥耦合措施。以10年生‘新林1号’杨为研究对象,设置-20 k Pa(I_(20))、-33 k Pa(I_(33))、-45 k Pa(I_(45))、未灌溉(I_0)4个灌溉水平和尿素500 g·株^(-1)(N_(1))、尿素1000 g·株^(-1)(N_(2))、尿素1500 g·株^(-1)(N_(3))、复合肥500 g·株^(-1)(F_(1))、复合肥1000 g·株^(-1)(F_(2))、复合肥1500 g·株^(-1)(F_(3))、对照(CK)7个施肥水平,测定‘新林1号’杨树高、胸径及叶片的光合生理指标。结果表明:(1)灌溉和施肥显著增加了胸径、树高和材积年增量。I_(20)灌溉处理的胸径、树高和单株材积年增量最高,比未灌溉分别提高了18.6%、40.6%、60.6%。F_2施肥处理胸径、树高和单株材积年增量最大,分别达到了2.47 cm、2.09 m、0.097 m^(3),比未施肥分别提高了8.3%、7.8%、15.3%。其中I_(20)F_(2)处理‘新林1号’杨生长量最大,显著高于其他处理。(2)灌溉和施肥显著增加了叶片中叶绿素含量、可溶性糖含量、可溶性蛋白含量,提高了谷氨酰胺合成酶活性、硝酸还原酶活性、净光合速率及气孔导度,其中I_(20)F_(2)处理下可溶性蛋白含量、谷氨酰胺合成酶活性、硝酸还原酶活性和叶面积最高。(3)相关性分析结果表明,‘新林1号’杨胸径、树高和材积年增长量与叶片生理指标和净光合速率呈显著正相关,与胞间CO_(2)摩尔分数呈负相关。灌溉和施肥显著改变了叶片生理特性,提高了叶片光合能力,促进了‘新林1号’杨的生长。I_(20)F_(2)处理下,生理指标和净光合速率最高,林木生长达到了最佳水平。因此,保持水分充足(灌溉阈值为-20 k Pa)及施复合肥(1000 g·株^(-1))处理是改善‘新林1号’杨光合生理特征,促进林木生长的最佳水肥耦合措施。展开更多
文摘Proteomic approaches using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were adopted to identify proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen: Marssonina brunnea f. sp. multigermtubi. Proteins were extracted from leaves at 0, 12, 24, 48 and 72 hrs after pathogen inoculation. Five hundred reproducible expressed protein spots were detected. Forty-one differentially expressed protein spots resolved on the 2-DE gels were subjected to matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. The identified proteins were mainly related to photosynthesis, metabolism and defense response. Finally, proteins involved in plant defense mechanism were discussed.
文摘探究滴灌水肥耦合对‘新林1号’杨(Populus cathayana×canadansis‘Xinlin1’)生长和光合生理特性的影响,旨在选出促进‘新林1号’杨生长的最佳水肥耦合措施。以10年生‘新林1号’杨为研究对象,设置-20 k Pa(I_(20))、-33 k Pa(I_(33))、-45 k Pa(I_(45))、未灌溉(I_0)4个灌溉水平和尿素500 g·株^(-1)(N_(1))、尿素1000 g·株^(-1)(N_(2))、尿素1500 g·株^(-1)(N_(3))、复合肥500 g·株^(-1)(F_(1))、复合肥1000 g·株^(-1)(F_(2))、复合肥1500 g·株^(-1)(F_(3))、对照(CK)7个施肥水平,测定‘新林1号’杨树高、胸径及叶片的光合生理指标。结果表明:(1)灌溉和施肥显著增加了胸径、树高和材积年增量。I_(20)灌溉处理的胸径、树高和单株材积年增量最高,比未灌溉分别提高了18.6%、40.6%、60.6%。F_2施肥处理胸径、树高和单株材积年增量最大,分别达到了2.47 cm、2.09 m、0.097 m^(3),比未施肥分别提高了8.3%、7.8%、15.3%。其中I_(20)F_(2)处理‘新林1号’杨生长量最大,显著高于其他处理。(2)灌溉和施肥显著增加了叶片中叶绿素含量、可溶性糖含量、可溶性蛋白含量,提高了谷氨酰胺合成酶活性、硝酸还原酶活性、净光合速率及气孔导度,其中I_(20)F_(2)处理下可溶性蛋白含量、谷氨酰胺合成酶活性、硝酸还原酶活性和叶面积最高。(3)相关性分析结果表明,‘新林1号’杨胸径、树高和材积年增长量与叶片生理指标和净光合速率呈显著正相关,与胞间CO_(2)摩尔分数呈负相关。灌溉和施肥显著改变了叶片生理特性,提高了叶片光合能力,促进了‘新林1号’杨的生长。I_(20)F_(2)处理下,生理指标和净光合速率最高,林木生长达到了最佳水平。因此,保持水分充足(灌溉阈值为-20 k Pa)及施复合肥(1000 g·株^(-1))处理是改善‘新林1号’杨光合生理特征,促进林木生长的最佳水肥耦合措施。
