Platycodon grandiflorum A.DC.(PAl)C)root was taken as experiment material to extract polysaccharide.On the base of single factor tests(extraction time,extraction temperature,liquid-solid ratio,solvent pH value and NaC...Platycodon grandiflorum A.DC.(PAl)C)root was taken as experiment material to extract polysaccharide.On the base of single factor tests(extraction time,extraction temperature,liquid-solid ratio,solvent pH value and NaCl concentration),the study concluded the main factors affecting the extraction of PADC polysaccharide,which are liquid-solid ratio,extraction time and extraction temperature.Then through central-composite test design,the extraction conditions were concluded as liquid-solid ratio 34.43,extraction time 89.83 min and extraction temperature 52.47℃.By means of validation experiments,the adequacy of this model was confirmed.展开更多
A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus indu...A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus induction; MS medium containing 6\|BA 0.3 mg/L and IAA 0.1 mg/L was suitable for crowd bud propagation; 1/2 MS medium containing IAA 0.5 mg/L and ABT 0.1 mg/L is effective in inducing root with the ratio of 95%, fast propagation of P. grandiflorum by tissue culture method could be established, which will lay down the foundation of the breeding and clone of excellent varieties of P. grandiflorum.展开更多
文摘Platycodon grandiflorum A.DC.(PAl)C)root was taken as experiment material to extract polysaccharide.On the base of single factor tests(extraction time,extraction temperature,liquid-solid ratio,solvent pH value and NaCl concentration),the study concluded the main factors affecting the extraction of PADC polysaccharide,which are liquid-solid ratio,extraction time and extraction temperature.Then through central-composite test design,the extraction conditions were concluded as liquid-solid ratio 34.43,extraction time 89.83 min and extraction temperature 52.47℃.By means of validation experiments,the adequacy of this model was confirmed.
文摘A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus induction; MS medium containing 6\|BA 0.3 mg/L and IAA 0.1 mg/L was suitable for crowd bud propagation; 1/2 MS medium containing IAA 0.5 mg/L and ABT 0.1 mg/L is effective in inducing root with the ratio of 95%, fast propagation of P. grandiflorum by tissue culture method could be established, which will lay down the foundation of the breeding and clone of excellent varieties of P. grandiflorum.
