To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four differen...To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four different types of silicon formulations,Si-50-G,Si-60-G,Si-T-G,and Si-E-G,were applied as foliar sprays at the seedling stage,and a control group CK(equal amount of distilled water)was set up.One week after the first silicon spray,two types of rice were subjected to low-temperature stress treatments at day/night temperatures of 12℃/10℃for 2,4,6,and 8 days.The effects of different silicon formulations on the chlorophyll,proline(Pro)and soluble sugar contents as well as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of rice seedlings under low-temperature stress were compared to find out the effects of silicon formulations on the cold tolerance of rice seedlings.The results showed that silicon formulations could significantly increase the chlorophyll content of rice seedling leaves,with Si-50-G being the most effective,with a significant increment of 40.17%compared to the CK at 2 days of low temperature.Four silicon formulations significantly increased the proline content and soluble sugar content of rice leaves at low temperature for 4-8 days.For Song Japonica 16,the most significant increment in leaf POD activity was observed in Si-E-G treatment at 2,4 and 8 days of temperature stress,with 73.58%,20.95%and 217.24%increases compared to the CK,respectively.For 4 and 6 days of temperature stress,the most significant increase in CAT activity was observed in Si-E-G treatment,with 25.70%and 75.78%increases compared to the CK,respectively.For Dongnong 427,the Si-60-G treatment showed the highest increase in leaf SOD activity for 4 and 8 days of temperature stress,with significant increases of 58.15%and 82.76%compared to the CK,respectively,and the Si-E-G treatment showed the highest increase in leaf POD activity for 2 and 8 days of temperature stress,with significant increases of 97.75%and 245.10%compared to the CK,respectively.It showed that the spraying of silicon formulations could significantly enhance the cold tolerance of rice.This study provided a scientific basis for the rational use of silicon formulations to enhance cold tolerance in rice and had important theoretical and practical significance for ensuring sustainable high and stable rice yields in Heilongjiang Province,as well as for the development of silicon fertilizers.展开更多
In order to investigate the physiological injury of barley caused by the low temperature after herbicides, tillering barley seedlings planted in plastic cups were pretreated in illumination incubator at 15℃ and 12 h-...In order to investigate the physiological injury of barley caused by the low temperature after herbicides, tillering barley seedlings planted in plastic cups were pretreated in illumination incubator at 15℃ and 12 h-light per day for 7 days, and then subjected to herbicide treatment, prometryn （with the concentrations of 0, 0.15%, 0.30%, and 0.45%） or isoproturon （with the concentrations of 0, 0.30%, 0.60%, and 0.90%）, and the SOD activity, the CAT activity, the POD activity, the MDA content, proline content, soluble protein content, electrical conductivity, and the rate of O2 were determined and analyzed. The results showed that the low-temperature was the most important, and the treatment-time of low-temperature was another significant influencing factor on the physiological and biochemical indices of barley seedlings. However, all of the physiological and biochemical indices determined were not affected by the kinds of herbicides and herbicide concentrations, and the SOD was stable and should play the more prominent role on extracting of free radicals according to the stepwise regression and correlation. The herbicide concentrations should be increased in the future research for truly reflecting the effects of the herbicide concentration on the physiological and biochemical indices of barley seedlings.展开更多
基金the National Natural Science Foundation of China(31471440)。
文摘To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four different types of silicon formulations,Si-50-G,Si-60-G,Si-T-G,and Si-E-G,were applied as foliar sprays at the seedling stage,and a control group CK(equal amount of distilled water)was set up.One week after the first silicon spray,two types of rice were subjected to low-temperature stress treatments at day/night temperatures of 12℃/10℃for 2,4,6,and 8 days.The effects of different silicon formulations on the chlorophyll,proline(Pro)and soluble sugar contents as well as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of rice seedlings under low-temperature stress were compared to find out the effects of silicon formulations on the cold tolerance of rice seedlings.The results showed that silicon formulations could significantly increase the chlorophyll content of rice seedling leaves,with Si-50-G being the most effective,with a significant increment of 40.17%compared to the CK at 2 days of low temperature.Four silicon formulations significantly increased the proline content and soluble sugar content of rice leaves at low temperature for 4-8 days.For Song Japonica 16,the most significant increment in leaf POD activity was observed in Si-E-G treatment at 2,4 and 8 days of temperature stress,with 73.58%,20.95%and 217.24%increases compared to the CK,respectively.For 4 and 6 days of temperature stress,the most significant increase in CAT activity was observed in Si-E-G treatment,with 25.70%and 75.78%increases compared to the CK,respectively.For Dongnong 427,the Si-60-G treatment showed the highest increase in leaf SOD activity for 4 and 8 days of temperature stress,with significant increases of 58.15%and 82.76%compared to the CK,respectively,and the Si-E-G treatment showed the highest increase in leaf POD activity for 2 and 8 days of temperature stress,with significant increases of 97.75%and 245.10%compared to the CK,respectively.It showed that the spraying of silicon formulations could significantly enhance the cold tolerance of rice.This study provided a scientific basis for the rational use of silicon formulations to enhance cold tolerance in rice and had important theoretical and practical significance for ensuring sustainable high and stable rice yields in Heilongjiang Province,as well as for the development of silicon fertilizers.
基金Supported by the National Natural Science Foundation of China (31000712)
文摘In order to investigate the physiological injury of barley caused by the low temperature after herbicides, tillering barley seedlings planted in plastic cups were pretreated in illumination incubator at 15℃ and 12 h-light per day for 7 days, and then subjected to herbicide treatment, prometryn （with the concentrations of 0, 0.15%, 0.30%, and 0.45%） or isoproturon （with the concentrations of 0, 0.30%, 0.60%, and 0.90%）, and the SOD activity, the CAT activity, the POD activity, the MDA content, proline content, soluble protein content, electrical conductivity, and the rate of O2 were determined and analyzed. The results showed that the low-temperature was the most important, and the treatment-time of low-temperature was another significant influencing factor on the physiological and biochemical indices of barley seedlings. However, all of the physiological and biochemical indices determined were not affected by the kinds of herbicides and herbicide concentrations, and the SOD was stable and should play the more prominent role on extracting of free radicals according to the stepwise regression and correlation. The herbicide concentrations should be increased in the future research for truly reflecting the effects of the herbicide concentration on the physiological and biochemical indices of barley seedlings.
