This article addresses the response of GLAss fiber REinforced aluminum to hypervelocity impacts of micrometeoroid analogs at impact velocities of 7 km/s and beyond.In relation,the damage modes of different GLAss fiber...This article addresses the response of GLAss fiber REinforced aluminum to hypervelocity impacts of micrometeoroid analogs at impact velocities of 7 km/s and beyond.In relation,the damage modes of different GLAss fiber REinforced aluminum configurations have been exemplified.The GLAss fiber REinforced aluminum configurations comprised six to twelve variably thick aluminum layers and up to four plies of glass fiber reinforced epoxy per composite laminate.Hypervelocity impact experiments have been conducted with the help of a two-stage light-gas gun,wherein aluminum-and stainless steel projectiles were launched at velocities up to 7.15 km/s.Visual inspection of the damage area suggested the dissipation of impact energy in elastic-plastic deformation,petalling,delamination,debonding,tensile failure of fibers,and pyrolysis of epoxy.A prevailing damage mode was not apparent albeit.The quasi-isotropic ply orientation of S2-glass/FM94-epoxy laminates promoted the interference of shockand rarefaction waves and suppressed the damage area of GLAss fiber REinforced aluminum.To discriminate between the impact performance of different GLAss fiber REinforced aluminum configurations,the energy dissipated in different damage modes of GLAss fiber REinforced aluminum has been assessed quantitatively.In terms of normalized energy,the cross-ply GLAss fiber REinforced aluminum dissipated higher energy in petal formation than in other primary damage modes.The normalized petalling energy was found to decline with the increase of impact energy.The outcomes of this study will help to optimize the GLAss fiber REinforced aluminum laminate,which will be employed as a bumper shield to prevent the fatal damage and the unzipping of a spacecraft pressure bulkhead.展开更多
Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ...Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.展开更多
A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference ...A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools.展开更多
基金funded by Bangladesh Air Force (BAF) Academy,Bangladesh
文摘This article addresses the response of GLAss fiber REinforced aluminum to hypervelocity impacts of micrometeoroid analogs at impact velocities of 7 km/s and beyond.In relation,the damage modes of different GLAss fiber REinforced aluminum configurations have been exemplified.The GLAss fiber REinforced aluminum configurations comprised six to twelve variably thick aluminum layers and up to four plies of glass fiber reinforced epoxy per composite laminate.Hypervelocity impact experiments have been conducted with the help of a two-stage light-gas gun,wherein aluminum-and stainless steel projectiles were launched at velocities up to 7.15 km/s.Visual inspection of the damage area suggested the dissipation of impact energy in elastic-plastic deformation,petalling,delamination,debonding,tensile failure of fibers,and pyrolysis of epoxy.A prevailing damage mode was not apparent albeit.The quasi-isotropic ply orientation of S2-glass/FM94-epoxy laminates promoted the interference of shockand rarefaction waves and suppressed the damage area of GLAss fiber REinforced aluminum.To discriminate between the impact performance of different GLAss fiber REinforced aluminum configurations,the energy dissipated in different damage modes of GLAss fiber REinforced aluminum has been assessed quantitatively.In terms of normalized energy,the cross-ply GLAss fiber REinforced aluminum dissipated higher energy in petal formation than in other primary damage modes.The normalized petalling energy was found to decline with the increase of impact energy.The outcomes of this study will help to optimize the GLAss fiber REinforced aluminum laminate,which will be employed as a bumper shield to prevent the fatal damage and the unzipping of a spacecraft pressure bulkhead.
基金Supported by Heilongjiang Province Science and Technology Research Projects (GB06B112-5)
文摘Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.
基金Supported by Harbin Science and Technology Bureau(2016RQYXJ018,2017RAQXJ104)the Key Laboratory of Soybean Biology in the Chinese Ministry of Education,Northeast Agricultural University(SB17A01)+3 种基金the National Natural Science Foundation of China(31801386)Heilongjiang Natural Science Foundation(LC2018008)Heilongjiang General Young Innovative Talents Training Plan(UNPYSCT-2018158)Certificate of China Postdoctoral Science Foundation Grant(2018M641839)
文摘A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools.
