Using ginseng (Panax ginseng C. A. Mey.) hairy roots as a bioreactor, exogenous hydroquinone (HQ) was added aseptically to suspension of hairy roots on 22 d. Twenty-four hours after the addition of HQ, the hairy roots...Using ginseng (Panax ginseng C. A. Mey.) hairy roots as a bioreactor, exogenous hydroquinone (HQ) was added aseptically to suspension of hairy roots on 22 d. Twenty-four hours after the addition of HQ, the hairy roots were separated from the medium. Through identification of TLC and HPLC,it was proved that hydroquinone has been bioconversed into arbutin, and arbutin from hairy root of P. ginseng was isolated and identified.展开更多
The soluble protein of Panax quinquelium L. and P. gingseng C. A. Mey were analyzed by mean of PAGE. When the concentration of separating gel is 12%, P. quinquelium presents a characteristic strip C3 (Mw 150 00), whil...The soluble protein of Panax quinquelium L. and P. gingseng C. A. Mey were analyzed by mean of PAGE. When the concentration of separating gel is 12%, P. quinquelium presents a characteristic strip C3 (Mw 150 00), while P. gingseng presents two characteristic strips A1 (Mw 927 00) and C2 (Mw 194 00). This method is used for distinguishing P. quinquelium and P. gingseng simplely.展开更多
ATP content of cultured callus and hairy root introducted by Ri plasmid of \%Panax ginseng \%C. A. Mey was detected by bioluminescence. The result showed that ATP content in hairy root is obviously higher than that in...ATP content of cultured callus and hairy root introducted by Ri plasmid of \%Panax ginseng \%C. A. Mey was detected by bioluminescence. The result showed that ATP content in hairy root is obviously higher than that in callus within a growth cycle. The highest ATP content in hairy root is 26.6×10\+\{-12\}mmol/g (fresh weight), while ATP in callus is 2.68×10\+\{-12\}mmol/g (fresh weight). Total saponin content in hairy root reaches 2.486% (dry weight), while the saponin in callus is 1.105% (dry weight). The hairy root inducted by Ri plasmid has vigorous ability in secondary metabolism for ginseng saponin synthesis.展开更多
文摘Using ginseng (Panax ginseng C. A. Mey.) hairy roots as a bioreactor, exogenous hydroquinone (HQ) was added aseptically to suspension of hairy roots on 22 d. Twenty-four hours after the addition of HQ, the hairy roots were separated from the medium. Through identification of TLC and HPLC,it was proved that hydroquinone has been bioconversed into arbutin, and arbutin from hairy root of P. ginseng was isolated and identified.
文摘The soluble protein of Panax quinquelium L. and P. gingseng C. A. Mey were analyzed by mean of PAGE. When the concentration of separating gel is 12%, P. quinquelium presents a characteristic strip C3 (Mw 150 00), while P. gingseng presents two characteristic strips A1 (Mw 927 00) and C2 (Mw 194 00). This method is used for distinguishing P. quinquelium and P. gingseng simplely.
文摘ATP content of cultured callus and hairy root introducted by Ri plasmid of \%Panax ginseng \%C. A. Mey was detected by bioluminescence. The result showed that ATP content in hairy root is obviously higher than that in callus within a growth cycle. The highest ATP content in hairy root is 26.6×10\+\{-12\}mmol/g (fresh weight), while ATP in callus is 2.68×10\+\{-12\}mmol/g (fresh weight). Total saponin content in hairy root reaches 2.486% (dry weight), while the saponin in callus is 1.105% (dry weight). The hairy root inducted by Ri plasmid has vigorous ability in secondary metabolism for ginseng saponin synthesis.
