为了理解荒漠短命植物小车前(Plantago minuta Pall.)种子表面的粘液物质对种子在干旱环境中萌发的作用,在室内控制条件下研究了粘液物质的吸水保水特性、剥离粘液物质的种子(无粘液种子)和保留粘液物质的种子(粘液种子)在-1.15^-0.15MP...为了理解荒漠短命植物小车前(Plantago minuta Pall.)种子表面的粘液物质对种子在干旱环境中萌发的作用,在室内控制条件下研究了粘液物质的吸水保水特性、剥离粘液物质的种子(无粘液种子)和保留粘液物质的种子(粘液种子)在-1.15^-0.15MPa不同渗透胁迫条件下的萌发状况。结果表明:(1)小车前种子表面的粘液物质干重占粘液种子干重的5.6%;在去离子水中,粘液物质可以吸收相当于其自身干重217.1倍的水分;粘液物质的存在使得小车前种子的吸水倍数由2倍增加至14倍,从而保证为种子萌发提供充足的水分;(2)干燥的粘液种子吸水2h后即达到饱和,而吸水饱和后的粘液种子在室温下晾置,经过48h后又干燥失水恢复至原重;(3)在去离子水中或低渗透胁迫(-0.33^-0.15MPa)条件下,粘液物质吸水作用能促进小车前种子萌发,粘液种子3d的发芽势和10d的总萌发率均显著高于无粘液种子;在高渗透胁迫(-1.15^-0.73MPa)条件下,粘液种子3d的发芽势显著低于无粘液种子的,而10d的总萌发率与无粘液种子的没有显著差别,表明粘液物质在干旱条件下可能从种子而非外界环境中吸取水分,减缓了种子萌发速率。通过上述结果可以认为小车前种子表面的粘液物质在早春干湿交替剧烈的荒漠生境中起到通过调节水分来调节种子萌发的作用,这种萌发策略大大降低了因大批种子同时萌发导致幼苗受旱甚至种群灭亡的潜在风险。展开更多
Aim Ethnopharmacological relevance： Ribes diacanthum Pall. （Saxifragaceae） , a Mongolian folk me- dicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effect...Aim Ethnopharmacological relevance： Ribes diacanthum Pall. （Saxifragaceae） , a Mongolian folk me- dicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effects of Ribes diacanthum Pall （RDP） against cisplatin-induced nephrotoxicity. Methods The renal injury was mod- eled by intraperitoneal injection of cisplatin for 5 consecutive days （5 mg·kg^-1 ）. Nephroprotection of RDP was investigated by oral administration of RDP aqueous extract at a daily dose of 40 mg · kg^- 1 for 14 consecutive days, starting 7 days prior to cisplatin administration. Results We demonstrated that pretreatment with RDP aqueous ex- tract protected the mice from death induced by cisplatin administration. RDP treatment also significantly reduced blood urea nitrogen （BUN） and serum creatinine （Cr） levels observed in cisplatin-administrated mice. Histopatho- logical analysis demonstrated that RDP administration protected cisplatin-induced renal tubular cell apoptosis. Fur- ther western blotting analysis revealed that RDP significantly reversed cisplatin-increased expression levels of cleaved-Caspase-3, Bax and cisplatin-decreased expression level of Bcl-2 in renal tissue. Finally, RDP markedly enhanced enzyme activities of reduced superoxide dismutase （SOD）, Heine oxygenase 1 （HO-1） and catalase （CAT）, suppressed lipid peroxidation as well as reactive oxygen species （ROS） production. Conclusion We concluded that RDP displayed nephroprotective effects against cisplatin-induced renal tubular cell apoptosis, possi- bly associated with both enhanced antioxidase activity and suppressed ROS generation. Given the major nephrotox- icity of cisplatin cancer chemotherapy, RDP might be a potential candidate for neoadjuvant chemotherapy.展开更多
OBJECTIVE This work aimed to investigate the anti-rheumatoid arthritic effect of gentio.picroside from Gentiana macrophylla Pall using an animal model of adjuvant induced arthritis.METH.ODS Adjuvant arthritis was indu...OBJECTIVE This work aimed to investigate the anti-rheumatoid arthritic effect of gentio.picroside from Gentiana macrophylla Pall using an animal model of adjuvant induced arthritis.METH.ODS Adjuvant arthritis was induced in fifty SD male rats,which were randomly divided into five groups(n=10):control(0.5% CMC-Na) group,AIA(rats with CFA) group,dexamethasone(1 mg·kg^(-1)) group,gentiopicroside(50 mg·kg^(-1)) group,and gentiopicroside(100 mg·kg^(-1)) group.Rats were administered intragastrically with drugs or CMC-Na once a day for a period of 2 weeks.Paw swelling,arthritic index,histological changes were assessed to evaluate the anti-arthritic effect.Weight growth,spleen and thymus indexes were also investigated in.RESULTS Gentiopicroside at dose of 100 mg·kg^(-1) significantly inhibited the secondary paw swelling(P<0.05) and arthritis index(P<0.05),decreased synovial inflammatory infil.tration,synovial hyperplasia and bone erosion.Furthermore,gentiopicroside showed no immunosup.pressive adverse effects in body weight,index of spleen and thyums compared with dexamethasone administration(P<0.05,P<0.01).CONCLUSION Gentiopicroside possessed anti-arthritic efficacy in AIA rats without immunosuppressive effects.展开更多
文摘为了理解荒漠短命植物小车前(Plantago minuta Pall.)种子表面的粘液物质对种子在干旱环境中萌发的作用,在室内控制条件下研究了粘液物质的吸水保水特性、剥离粘液物质的种子(无粘液种子)和保留粘液物质的种子(粘液种子)在-1.15^-0.15MPa不同渗透胁迫条件下的萌发状况。结果表明:(1)小车前种子表面的粘液物质干重占粘液种子干重的5.6%;在去离子水中,粘液物质可以吸收相当于其自身干重217.1倍的水分;粘液物质的存在使得小车前种子的吸水倍数由2倍增加至14倍,从而保证为种子萌发提供充足的水分;(2)干燥的粘液种子吸水2h后即达到饱和,而吸水饱和后的粘液种子在室温下晾置,经过48h后又干燥失水恢复至原重;(3)在去离子水中或低渗透胁迫(-0.33^-0.15MPa)条件下,粘液物质吸水作用能促进小车前种子萌发,粘液种子3d的发芽势和10d的总萌发率均显著高于无粘液种子;在高渗透胁迫(-1.15^-0.73MPa)条件下,粘液种子3d的发芽势显著低于无粘液种子的,而10d的总萌发率与无粘液种子的没有显著差别,表明粘液物质在干旱条件下可能从种子而非外界环境中吸取水分,减缓了种子萌发速率。通过上述结果可以认为小车前种子表面的粘液物质在早春干湿交替剧烈的荒漠生境中起到通过调节水分来调节种子萌发的作用,这种萌发策略大大降低了因大批种子同时萌发导致幼苗受旱甚至种群灭亡的潜在风险。
文摘Aim Ethnopharmacological relevance： Ribes diacanthum Pall. （Saxifragaceae） , a Mongolian folk me- dicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effects of Ribes diacanthum Pall （RDP） against cisplatin-induced nephrotoxicity. Methods The renal injury was mod- eled by intraperitoneal injection of cisplatin for 5 consecutive days （5 mg·kg^-1 ）. Nephroprotection of RDP was investigated by oral administration of RDP aqueous extract at a daily dose of 40 mg · kg^- 1 for 14 consecutive days, starting 7 days prior to cisplatin administration. Results We demonstrated that pretreatment with RDP aqueous ex- tract protected the mice from death induced by cisplatin administration. RDP treatment also significantly reduced blood urea nitrogen （BUN） and serum creatinine （Cr） levels observed in cisplatin-administrated mice. Histopatho- logical analysis demonstrated that RDP administration protected cisplatin-induced renal tubular cell apoptosis. Fur- ther western blotting analysis revealed that RDP significantly reversed cisplatin-increased expression levels of cleaved-Caspase-3, Bax and cisplatin-decreased expression level of Bcl-2 in renal tissue. Finally, RDP markedly enhanced enzyme activities of reduced superoxide dismutase （SOD）, Heine oxygenase 1 （HO-1） and catalase （CAT）, suppressed lipid peroxidation as well as reactive oxygen species （ROS） production. Conclusion We concluded that RDP displayed nephroprotective effects against cisplatin-induced renal tubular cell apoptosis, possi- bly associated with both enhanced antioxidase activity and suppressed ROS generation. Given the major nephrotox- icity of cisplatin cancer chemotherapy, RDP might be a potential candidate for neoadjuvant chemotherapy.
文摘OBJECTIVE This work aimed to investigate the anti-rheumatoid arthritic effect of gentio.picroside from Gentiana macrophylla Pall using an animal model of adjuvant induced arthritis.METH.ODS Adjuvant arthritis was induced in fifty SD male rats,which were randomly divided into five groups(n=10):control(0.5% CMC-Na) group,AIA(rats with CFA) group,dexamethasone(1 mg·kg^(-1)) group,gentiopicroside(50 mg·kg^(-1)) group,and gentiopicroside(100 mg·kg^(-1)) group.Rats were administered intragastrically with drugs or CMC-Na once a day for a period of 2 weeks.Paw swelling,arthritic index,histological changes were assessed to evaluate the anti-arthritic effect.Weight growth,spleen and thymus indexes were also investigated in.RESULTS Gentiopicroside at dose of 100 mg·kg^(-1) significantly inhibited the secondary paw swelling(P<0.05) and arthritis index(P<0.05),decreased synovial inflammatory infil.tration,synovial hyperplasia and bone erosion.Furthermore,gentiopicroside showed no immunosup.pressive adverse effects in body weight,index of spleen and thyums compared with dexamethasone administration(P<0.05,P<0.01).CONCLUSION Gentiopicroside possessed anti-arthritic efficacy in AIA rats without immunosuppressive effects.
