Objective:To evaluate effects of diagnostic tests for Helicobacter pylori(H.pylori)infec- tion.Methods:A meta-analysis was conducted in 22 identified studies through Chinese literature searching which were published a...Objective:To evaluate effects of diagnostic tests for Helicobacter pylori(H.pylori)infec- tion.Methods:A meta-analysis was conducted in 22 identified studies through Chinese literature searching which were published after 1995 and evaluated diagnostic tests for Helicobacter pylori(H.pylori)infec- tion.Results:Polymerase chain reaction(PCR)had the best performance with diagnostic odds ratio (DOR)of 6.7(5.5-7.8),followed by ^(13)C urea breath test and Enzyme-linked immunosorbent assay (ELISA)quantitative serological test,with DOR being 6.4(5.4-7.4)and 4.5(3.8-5.2),respectively. Conclusion:Non-invasive tests are the appropriate methods for screening H.pylori infection,whereas in- vasive tests are the best methods for ascertaining the suspected patients.展开更多
MéNéTRIER＇S disease （MD） is a rare diseasecharacterized by hypertrophy of gastricfolds, and nausea, emesis, abdominal painand peripheral oedema are the mainmanifestations. Hypochlorhydria and hypoalbumine...MéNéTRIER＇S disease （MD） is a rare diseasecharacterized by hypertrophy of gastricfolds, and nausea, emesis, abdominal painand peripheral oedema are the mainmanifestations. Hypochlorhydria and hypoalbuminemiaare also common.1 MD is always misdiagnosed due to itsrarity. Especially, the symptoms caused by hypoalbuminemia,for example, pleural effusions or ascites,make the correct diagnosis more difficult.展开更多
A nested polymerase chain reaction(N-PCR)for the spegific detection of Helicobacterpylori(H.pylori)was developed with two primer pairs(nested primers)derived from ureasegene A of H.pylori.The N-PCR was used to detect ...A nested polymerase chain reaction(N-PCR)for the spegific detection of Helicobacterpylori(H.pylori)was developed with two primer pairs(nested primers)derived from ureasegene A of H.pylori.The N-PCR was used to detect 21 different samples of H.pylori including20 clinical isolates and 1 reference strain NCTC 14126,but it was negative for other bacterialspecies,showing the N-PCR assay to be 100% specific.Tenfold serial dilution experiments re-vealed the detection of as little as 0.1 fg of H.pylori DNA by N-PCR.To evaluate the PCR as-say for clinical samples,gastric biopsies were tested with N-PCR,and the results were comparedwith those of culture,urease test and histologic examination(reference standard,RS).In 30biopsy specimens,H.pylori DNA sequences were detected by PCR in all of 20(100%)positivetissue and none of the 10 negative tissues.PCR is a specific and sensitive method that can detectthe presence of H.pylori without the need for culture and would have significant importance di-agnostically and epidemiologically.展开更多
Ninety-two asymptomafic subjects were investigated to determine whether they were in-fected with Helicobacter pylori(HP)by means of fiber gastroscopy, urease test andWarthin-Starry silver stain.As a result of this wor...Ninety-two asymptomafic subjects were investigated to determine whether they were in-fected with Helicobacter pylori(HP)by means of fiber gastroscopy, urease test andWarthin-Starry silver stain.As a result of this work,49 subjects with HP infection were found.Under gastroscope,24 of 92 subjects had normal gastric mucosa 45 suffered frompiebaldism-like congestion of the gastric mucosa and 23 mucosal erosion,but the differencesamong the detectable rotes of them had no statistical significance(P】0.05).Forty-six of the subjects with HP infection were seen in the 67 patients with gastritis and only 3 in the subjectswith normal mucosa.The positive rates of HP infection in the patients with moderate and serfous gastritis were significant highly(P【0.01),as compared with that in mild gastritis.It could besuggested that HP infection and the gastritis associated with it may universally exist in“healthy persons”without symptom.展开更多
Objective: To determine whether attenuated Salmonella typhimurium producing Helicobacter pylori (Hp) urease subunit B(UreB)can elicit specific immune responses against Hp in mice tested by a lavage technique. Methods:...Objective: To determine whether attenuated Salmonella typhimurium producing Helicobacter pylori (Hp) urease subunit B(UreB)can elicit specific immune responses against Hp in mice tested by a lavage technique. Methods: Attenuated Salmonella typhimurium producing Hp UreB immunized orally Balb/c mice twice at a 3-week interval. After 12 weeks,mice intestinal secretions were obtained without harm by administering a lavage solution intragastrically. The mice intestinal secretions of immune group were also directly washed out after the mice were killed. The antibody responses were evaluated by using serum and intestinal fluid with ELISA assay. Results: The multiple oral immunizations with SL3261/ pTC01-UreB induced significantly Hp-specific mucosal IgA response as well as serum IgG response. The IgA was also consistently higher in the intestinal fluid obtained by the lavage solution than by direct washout. In addition, no obvious side effects and changes in gastric inflammation were observed in mice. Conclusion: The attenuated Salmonella typhimurium expressing Hp UreB may be used as an oral vaccine against Hp infection. And the lavage technique is an ideal method in the study of mucosal immune responses.展开更多
Objective: To investigate the status of cagE gene of Helicobacter pylori(H. pylori) isolated from patients with various gastrointestinal diseases and its relationship with the pathological inflammation grade and level...Objective: To investigate the status of cagE gene of Helicobacter pylori(H. pylori) isolated from patients with various gastrointestinal diseases and its relationship with the pathological inflammation grade and levels of IL 8 in the gastric mucosa and IL 8 secretion in gastric epithelial cells stimulated by H. pylori . Methods: cagE was amplified by polymerase chain reaction (PCR) in 145 clinical isolates. The inflammation grade of gastric mucosa was evaluated pathologically. IL 8 levels of gastric mucosa and IL 8 concentration of the supernatant of the cocultured SGC 7 901 cells and H. pylori was assayed by enzyme linked immunosorbent assay (ELISA). Results: cagE was positive in 79.3% of all the H. pylori strains. The mean score of the cagE positive gastritis in the antrum and corpus was (1.865±0.335) and (1.759±0.310). Meanwhile, the cagE negative grade was (1.689±0.294), (1.608±0.284). There was no significant difference ( P >0 05). The mean levels of IL 8 in cagE positive group in antrum and corpus were (390.6±101.4) pg/mg and (368.6±91.2) pg/mg; and cagE negative group were (328.6±102.8) pg/mg and (332.6±96.7) pg/mg. IL 8 in SGC7901 cells induced by cagE positive and negative group averaged (789.5±146.7) pg/ml and (757.6±136.4) pg/ml. There was still no significant difference( P >0.05). Conclusion: Positive rate of cagE is very high in Chinese patients regardless of the clinical outcome. And there was no direct relationship between cagE gene and the inflammation grade and IL 8 levels of H. pylori infected gastric mucosa and IL 8 secretion in gastric epithelial cells stimulated by H. pylori .展开更多
Objective: To study the relationship between nitric oxide synthase (NOS) expression in human gastric mucosa and Helicobacter pylori (H.pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33...Objective: To study the relationship between nitric oxide synthase (NOS) expression in human gastric mucosa and Helicobacter pylori (H.pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33 patients received gastroendoscopy. H.pylori infection was confirmed by Giems staining and bacteria culture under microaerophilic conditions. Expression of iNOS, eNOS and nitrotyrosine were detected by immunohistochemistry. Results: (1) The positive rate of H. pylori infection was 66.7%(22/33). (2) iNOS positive staining in inflammatory cells was detected in 77.3%(17/22) of samples with H.pylori and 27.3%(3/11) without H.pylori infection (P<0.01). (3) eNOS expression in inflammatory cells was found in 77.3%(17/22) of samples with H. pylori and 18.2%(2/11) without H.pylori infection (P<0.01). (4) Nitrotyrosine expression in inflammatory cells was observed in 59.1%(13/22) of samples with H. pylori and 54.5%(6/11) without H. pylori infection (P>0.05). (5) Moderate and severe infiltrations of inflammatory cells were found in 86.4%(19/22) of gastric biopsies with H. pylori and 9.1%(1/11) of samples without H. pylori infection (P<0.01). Conclusion: H.pylori infection might promote infiltration of mononuclear cells and macrophages in gastric mucosa and induce iNOS expression in these cells. The accumulated nitric oxide in local area may result in gastric mucosa damage.展开更多
Objective.To evaluate the clinical utility of a new non-invasive enzyme immunoassay(EIA)for the diagnosis of Helicobacter pylori(H.pylori)infection.Methods.Stool specimens of63patients were collected and tested by usi...Objective.To evaluate the clinical utility of a new non-invasive enzyme immunoassay(EIA)for the diagnosis of Helicobacter pylori(H.pylori)infection.Methods.Stool specimens of63patients were collected and tested by using a commercial kit for detecting Helicobacter pylori stool antigen(HpSA),of which61patients also underwent 13 C-Urea breath test( 13 C-UBT).The tissue samples of31patients were obtained endoscopically and were examined with histologic technique(Warthin-Starry silver stain).Regarded 13 C-UBT as a golden standard,HpSA test and histologic techniques were evaluated.Using this method,we also investigated the positive rate of H.pylori infection in children in Beijing.Results.The sensitivity and specificity of HpSA test were94.7%and95.1%respectively;the posi-tive and negative predictive values were97.3%and91.7%respectively;and the accuracy was95.1%.The results showed the prevalence of H.pylori infection was26.0%in children(3～18years)of district of Xicheng in Beijing.After treatment ,HpSA seems to disappear rapidly(3～5days)from the feces.Conclusion.The detection of HpSA in stool samples by HpSA test is a rapid noninvasive test for detecting H.pylori infection,and has both high sensitivity and high specificity.It is suitable for screening and diagnosis of H.pylori infection,monitoring the treatment efficacy in routine in all hospitals.展开更多
Objective: To investigate the association between the diversity of urease gene and urease activity of clinical isolates of Helicobacter pylori (H. pylori). Methods: Polymerase chain reaction-restriction fragment lengt...Objective: To investigate the association between the diversity of urease gene and urease activity of clinical isolates of Helicobacter pylori (H. pylori). Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of urease gene and rapid urease activity test were used to study the urease activity of different clinical isolates of H. pylori. Results: H. pylori clinical isolates were divided into 4 types according to their PCR-RFLP results of urease gene and urease activity. Type Ⅰ , possessing strong urease activity (0. 11) and presented 1 fragment of 1. 7 kb by PCR-RFLP, had close relations with gastric ulcer; type Ⅱ , with the weakest urease activity (0. 07) and 2 fragments (1. 3 and 0. 4 kb respectively) , was associated with duodenal bulb ulcer; type Ⅲ , with the strongest urease activity (0. 12) and 2 fragments (0. 4 and 0. 17 kb) with or without 1 fragment (0. 23 or 0. 37 kb) , was responsible for gastritis; type Ⅳ , with weak urease activity (0. 09) and 2 fragments (1. 5 and 0. 2 kb), was shown to be related to both gastric and duodenal bulb ulcers. Conclusion: The diversity of urease gene decides different urease activities of different clinical isolates of H. pylori, hence the different possibilities of pathogenesis due to this bacteria.展开更多
Previous study has raised H' pylori infection as a suspected biologic risk factor for gastric cancer. Acomparative case-control study involving precancerous lesions and gastric cancer was conducted inYanzhong coun...Previous study has raised H' pylori infection as a suspected biologic risk factor for gastric cancer. Acomparative case-control study involving precancerous lesions and gastric cancer was conducted inYanzhong county, an area with one of the highest rates of gastric cancer in China to study the relatlonshipbetween the H' pylori infection and gastric cancer. Subjects in the study were all randomly selected Partici-pants of a screening program for gastric cancer sponsored by the Cancer Institute of CAMS in cooPerationwith Yangzhong county Hospital' Totally, l25 normal controls, lO8 superficial gastritis, l l1 atrophic gas-tritis and 110 gastric cancer patients were included in our study according to endoscopy and pathology re-sult. Status of H. pylori infection was evaluated by measuring Ig G antibody in plasma with ELISA assay.Our result showed Odds ratios of H' pylori infection were higher among gastritis and cancer groups, 4. 5(95 % CI 2. 5~7. 9) for superficial gastritis, 6. 3 (95% CI 3. 4~ 12) for atrophic gastritis, 3. 3 (95 % CI1. 9~5. 9) for gastric cancer. It was found in our study that consumption of pickled vegetables and drink-ing dirty water increased the relative risk of H' pylori lnfection for both precancerous lesions and gastriccancer and that H. pylori infection had higher risk of atrophic gastritis and gastric cancer for males and alsohigher risk of atrophic gastritis for olders. Our results strongIy support the casual role played by H. pyloriinfection in the carcinogenic process of gastric mucosa.展开更多
文摘Objective:To evaluate effects of diagnostic tests for Helicobacter pylori(H.pylori)infec- tion.Methods:A meta-analysis was conducted in 22 identified studies through Chinese literature searching which were published after 1995 and evaluated diagnostic tests for Helicobacter pylori(H.pylori)infec- tion.Results:Polymerase chain reaction(PCR)had the best performance with diagnostic odds ratio (DOR)of 6.7(5.5-7.8),followed by ^(13)C urea breath test and Enzyme-linked immunosorbent assay (ELISA)quantitative serological test,with DOR being 6.4(5.4-7.4)and 4.5(3.8-5.2),respectively. Conclusion:Non-invasive tests are the appropriate methods for screening H.pylori infection,whereas in- vasive tests are the best methods for ascertaining the suspected patients.
文摘MéNéTRIER＇S disease （MD） is a rare diseasecharacterized by hypertrophy of gastricfolds, and nausea, emesis, abdominal painand peripheral oedema are the mainmanifestations. Hypochlorhydria and hypoalbuminemiaare also common.1 MD is always misdiagnosed due to itsrarity. Especially, the symptoms caused by hypoalbuminemia,for example, pleural effusions or ascites,make the correct diagnosis more difficult.
文摘A nested polymerase chain reaction(N-PCR)for the spegific detection of Helicobacterpylori(H.pylori)was developed with two primer pairs(nested primers)derived from ureasegene A of H.pylori.The N-PCR was used to detect 21 different samples of H.pylori including20 clinical isolates and 1 reference strain NCTC 14126,but it was negative for other bacterialspecies,showing the N-PCR assay to be 100% specific.Tenfold serial dilution experiments re-vealed the detection of as little as 0.1 fg of H.pylori DNA by N-PCR.To evaluate the PCR as-say for clinical samples,gastric biopsies were tested with N-PCR,and the results were comparedwith those of culture,urease test and histologic examination(reference standard,RS).In 30biopsy specimens,H.pylori DNA sequences were detected by PCR in all of 20(100%)positivetissue and none of the 10 negative tissues.PCR is a specific and sensitive method that can detectthe presence of H.pylori without the need for culture and would have significant importance di-agnostically and epidemiologically.
文摘Ninety-two asymptomafic subjects were investigated to determine whether they were in-fected with Helicobacter pylori(HP)by means of fiber gastroscopy, urease test andWarthin-Starry silver stain.As a result of this work,49 subjects with HP infection were found.Under gastroscope,24 of 92 subjects had normal gastric mucosa 45 suffered frompiebaldism-like congestion of the gastric mucosa and 23 mucosal erosion,but the differencesamong the detectable rotes of them had no statistical significance(P】0.05).Forty-six of the subjects with HP infection were seen in the 67 patients with gastritis and only 3 in the subjectswith normal mucosa.The positive rates of HP infection in the patients with moderate and serfous gastritis were significant highly(P【0.01),as compared with that in mild gastritis.It could besuggested that HP infection and the gastritis associated with it may universally exist in“healthy persons”without symptom.
文摘Objective: To determine whether attenuated Salmonella typhimurium producing Helicobacter pylori (Hp) urease subunit B(UreB)can elicit specific immune responses against Hp in mice tested by a lavage technique. Methods: Attenuated Salmonella typhimurium producing Hp UreB immunized orally Balb/c mice twice at a 3-week interval. After 12 weeks,mice intestinal secretions were obtained without harm by administering a lavage solution intragastrically. The mice intestinal secretions of immune group were also directly washed out after the mice were killed. The antibody responses were evaluated by using serum and intestinal fluid with ELISA assay. Results: The multiple oral immunizations with SL3261/ pTC01-UreB induced significantly Hp-specific mucosal IgA response as well as serum IgG response. The IgA was also consistently higher in the intestinal fluid obtained by the lavage solution than by direct washout. In addition, no obvious side effects and changes in gastric inflammation were observed in mice. Conclusion: The attenuated Salmonella typhimurium expressing Hp UreB may be used as an oral vaccine against Hp infection. And the lavage technique is an ideal method in the study of mucosal immune responses.
文摘Objective: To investigate the status of cagE gene of Helicobacter pylori(H. pylori) isolated from patients with various gastrointestinal diseases and its relationship with the pathological inflammation grade and levels of IL 8 in the gastric mucosa and IL 8 secretion in gastric epithelial cells stimulated by H. pylori . Methods: cagE was amplified by polymerase chain reaction (PCR) in 145 clinical isolates. The inflammation grade of gastric mucosa was evaluated pathologically. IL 8 levels of gastric mucosa and IL 8 concentration of the supernatant of the cocultured SGC 7 901 cells and H. pylori was assayed by enzyme linked immunosorbent assay (ELISA). Results: cagE was positive in 79.3% of all the H. pylori strains. The mean score of the cagE positive gastritis in the antrum and corpus was (1.865±0.335) and (1.759±0.310). Meanwhile, the cagE negative grade was (1.689±0.294), (1.608±0.284). There was no significant difference ( P >0 05). The mean levels of IL 8 in cagE positive group in antrum and corpus were (390.6±101.4) pg/mg and (368.6±91.2) pg/mg; and cagE negative group were (328.6±102.8) pg/mg and (332.6±96.7) pg/mg. IL 8 in SGC7901 cells induced by cagE positive and negative group averaged (789.5±146.7) pg/ml and (757.6±136.4) pg/ml. There was still no significant difference( P >0.05). Conclusion: Positive rate of cagE is very high in Chinese patients regardless of the clinical outcome. And there was no direct relationship between cagE gene and the inflammation grade and IL 8 levels of H. pylori infected gastric mucosa and IL 8 secretion in gastric epithelial cells stimulated by H. pylori .
基金National Natural Science Foundation of China (No.30170427)
文摘Objective: To study the relationship between nitric oxide synthase (NOS) expression in human gastric mucosa and Helicobacter pylori (H.pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33 patients received gastroendoscopy. H.pylori infection was confirmed by Giems staining and bacteria culture under microaerophilic conditions. Expression of iNOS, eNOS and nitrotyrosine were detected by immunohistochemistry. Results: (1) The positive rate of H. pylori infection was 66.7%(22/33). (2) iNOS positive staining in inflammatory cells was detected in 77.3%(17/22) of samples with H.pylori and 27.3%(3/11) without H.pylori infection (P<0.01). (3) eNOS expression in inflammatory cells was found in 77.3%(17/22) of samples with H. pylori and 18.2%(2/11) without H.pylori infection (P<0.01). (4) Nitrotyrosine expression in inflammatory cells was observed in 59.1%(13/22) of samples with H. pylori and 54.5%(6/11) without H. pylori infection (P>0.05). (5) Moderate and severe infiltrations of inflammatory cells were found in 86.4%(19/22) of gastric biopsies with H. pylori and 9.1%(1/11) of samples without H. pylori infection (P<0.01). Conclusion: H.pylori infection might promote infiltration of mononuclear cells and macrophages in gastric mucosa and induce iNOS expression in these cells. The accumulated nitric oxide in local area may result in gastric mucosa damage.
文摘Objective.To evaluate the clinical utility of a new non-invasive enzyme immunoassay(EIA)for the diagnosis of Helicobacter pylori(H.pylori)infection.Methods.Stool specimens of63patients were collected and tested by using a commercial kit for detecting Helicobacter pylori stool antigen(HpSA),of which61patients also underwent 13 C-Urea breath test( 13 C-UBT).The tissue samples of31patients were obtained endoscopically and were examined with histologic technique(Warthin-Starry silver stain).Regarded 13 C-UBT as a golden standard,HpSA test and histologic techniques were evaluated.Using this method,we also investigated the positive rate of H.pylori infection in children in Beijing.Results.The sensitivity and specificity of HpSA test were94.7%and95.1%respectively;the posi-tive and negative predictive values were97.3%and91.7%respectively;and the accuracy was95.1%.The results showed the prevalence of H.pylori infection was26.0%in children(3～18years)of district of Xicheng in Beijing.After treatment ,HpSA seems to disappear rapidly(3～5days)from the feces.Conclusion.The detection of HpSA in stool samples by HpSA test is a rapid noninvasive test for detecting H.pylori infection,and has both high sensitivity and high specificity.It is suitable for screening and diagnosis of H.pylori infection,monitoring the treatment efficacy in routine in all hospitals.
基金Supported by National Natural Science Foundation of China (No. 39570334)
文摘Objective: To investigate the association between the diversity of urease gene and urease activity of clinical isolates of Helicobacter pylori (H. pylori). Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of urease gene and rapid urease activity test were used to study the urease activity of different clinical isolates of H. pylori. Results: H. pylori clinical isolates were divided into 4 types according to their PCR-RFLP results of urease gene and urease activity. Type Ⅰ , possessing strong urease activity (0. 11) and presented 1 fragment of 1. 7 kb by PCR-RFLP, had close relations with gastric ulcer; type Ⅱ , with the weakest urease activity (0. 07) and 2 fragments (1. 3 and 0. 4 kb respectively) , was associated with duodenal bulb ulcer; type Ⅲ , with the strongest urease activity (0. 12) and 2 fragments (0. 4 and 0. 17 kb) with or without 1 fragment (0. 23 or 0. 37 kb) , was responsible for gastritis; type Ⅳ , with weak urease activity (0. 09) and 2 fragments (1. 5 and 0. 2 kb), was shown to be related to both gastric and duodenal bulb ulcers. Conclusion: The diversity of urease gene decides different urease activities of different clinical isolates of H. pylori, hence the different possibilities of pathogenesis due to this bacteria.
文摘Previous study has raised H' pylori infection as a suspected biologic risk factor for gastric cancer. Acomparative case-control study involving precancerous lesions and gastric cancer was conducted inYanzhong county, an area with one of the highest rates of gastric cancer in China to study the relatlonshipbetween the H' pylori infection and gastric cancer. Subjects in the study were all randomly selected Partici-pants of a screening program for gastric cancer sponsored by the Cancer Institute of CAMS in cooPerationwith Yangzhong county Hospital' Totally, l25 normal controls, lO8 superficial gastritis, l l1 atrophic gas-tritis and 110 gastric cancer patients were included in our study according to endoscopy and pathology re-sult. Status of H. pylori infection was evaluated by measuring Ig G antibody in plasma with ELISA assay.Our result showed Odds ratios of H' pylori infection were higher among gastritis and cancer groups, 4. 5(95 % CI 2. 5~7. 9) for superficial gastritis, 6. 3 (95% CI 3. 4~ 12) for atrophic gastritis, 3. 3 (95 % CI1. 9~5. 9) for gastric cancer. It was found in our study that consumption of pickled vegetables and drink-ing dirty water increased the relative risk of H' pylori lnfection for both precancerous lesions and gastriccancer and that H. pylori infection had higher risk of atrophic gastritis and gastric cancer for males and alsohigher risk of atrophic gastritis for olders. Our results strongIy support the casual role played by H. pyloriinfection in the carcinogenic process of gastric mucosa.
