目的:探讨Wnt/PCP信号通路与细胞外基质(extracellular matrix,ECM)共同调控钙调蛋白2(calponin 2,CNN2)在促进肝癌细胞群体性迁移中的作用及其分子机制。方法:结合癌症基因组图谱(The Cancer Genome Atlas,TCGA)与人类蛋白图谱(Human P...目的:探讨Wnt/PCP信号通路与细胞外基质(extracellular matrix,ECM)共同调控钙调蛋白2(calponin 2,CNN2)在促进肝癌细胞群体性迁移中的作用及其分子机制。方法:结合癌症基因组图谱(The Cancer Genome Atlas,TCGA)与人类蛋白图谱(Human Protein Atlas,HPA)分析CNN2在不同组织中的表达特征,并通过组织芯片评估其在肝癌中的表达水平及预后相关性;采用迁移与划痕实验探讨CNN2对肝癌细胞迁移能力的影响,进一步通过shRNA慢病毒降低Wnt11及CNN2表达,研究Wnt/PCP信号通路对CNN2的调控作用及其分子机制;最后以硬质培养基模拟ECM环境,探讨其与CNN2表达及细胞迁移能力之间的关联。结果:CNN2在肝癌组织中高表达,富集于肿瘤边缘区域,提示其可能在肿瘤细胞迁移早期阶段发挥关键作用。功能实验进一步证实,CNN2显著促进肝癌细胞的群体性迁移,其高表达水平与患者不良预后显著相关。在机制层面,CNN2受Wnt/PCP信号通路调控,且在模拟ECM力学环境下表达显著上调,提示其在肿瘤细胞感知和响应力学信号过程中具有重要功能。结论:Wnt/PCP信号通路调控CNN2的表达与功能,同时CNN2作为力学信号介导因子受ECM力学信号调控,二者协同驱动肝癌细胞的群体性迁移。展开更多
The emission characteristics of HCl during the chloride waste combustion process are studied in this paper PVC, PCP, NaCl are fired separately or mixed with each other, or co fired with coal in a pipe stove The result...The emission characteristics of HCl during the chloride waste combustion process are studied in this paper PVC, PCP, NaCl are fired separately or mixed with each other, or co fired with coal in a pipe stove The results of the study indicate that chlorine in PVC and PCP would be emitted predominantly as HCl in a short time Chlorine in PVC could be released completely from 200 ℃ to 600 ℃.The conversion rate of chlorine to HCl from PCP is not as high as from PVC In addition, moisture is not the decisive factor that influences HCl emission Oxygen concentration promotes HCl formation very effectively In reactions related to coal, chlorine conversion for NaCl increases because of the effect of sulfur The formation rate of the chlorine molecule decreases when water vapor exists Even though oxygen is in excess, the formation rate is low, about 0 8%展开更多
文摘目的:探讨Wnt/PCP信号通路与细胞外基质(extracellular matrix,ECM)共同调控钙调蛋白2(calponin 2,CNN2)在促进肝癌细胞群体性迁移中的作用及其分子机制。方法:结合癌症基因组图谱(The Cancer Genome Atlas,TCGA)与人类蛋白图谱(Human Protein Atlas,HPA)分析CNN2在不同组织中的表达特征,并通过组织芯片评估其在肝癌中的表达水平及预后相关性;采用迁移与划痕实验探讨CNN2对肝癌细胞迁移能力的影响,进一步通过shRNA慢病毒降低Wnt11及CNN2表达,研究Wnt/PCP信号通路对CNN2的调控作用及其分子机制;最后以硬质培养基模拟ECM环境,探讨其与CNN2表达及细胞迁移能力之间的关联。结果:CNN2在肝癌组织中高表达,富集于肿瘤边缘区域,提示其可能在肿瘤细胞迁移早期阶段发挥关键作用。功能实验进一步证实,CNN2显著促进肝癌细胞的群体性迁移,其高表达水平与患者不良预后显著相关。在机制层面,CNN2受Wnt/PCP信号通路调控,且在模拟ECM力学环境下表达显著上调,提示其在肿瘤细胞感知和响应力学信号过程中具有重要功能。结论:Wnt/PCP信号通路调控CNN2的表达与功能,同时CNN2作为力学信号介导因子受ECM力学信号调控,二者协同驱动肝癌细胞的群体性迁移。
文摘The emission characteristics of HCl during the chloride waste combustion process are studied in this paper PVC, PCP, NaCl are fired separately or mixed with each other, or co fired with coal in a pipe stove The results of the study indicate that chlorine in PVC and PCP would be emitted predominantly as HCl in a short time Chlorine in PVC could be released completely from 200 ℃ to 600 ℃.The conversion rate of chlorine to HCl from PCP is not as high as from PVC In addition, moisture is not the decisive factor that influences HCl emission Oxygen concentration promotes HCl formation very effectively In reactions related to coal, chlorine conversion for NaCl increases because of the effect of sulfur The formation rate of the chlorine molecule decreases when water vapor exists Even though oxygen is in excess, the formation rate is low, about 0 8%
