隐私偏好平台(platform for privacy preferences,P3P)是现今世界上广泛使用的隐私策略语言之一,服务提供者通过P3P隐私策略来告知用户如何处理隐私信息。针对P3P隐私策略缺少形式化语义而难以被精确表达的问题,分析了P3P隐私策略对形...隐私偏好平台(platform for privacy preferences,P3P)是现今世界上广泛使用的隐私策略语言之一,服务提供者通过P3P隐私策略来告知用户如何处理隐私信息。针对P3P隐私策略缺少形式化语义而难以被精确表达的问题,分析了P3P隐私策略对形式化语义的需求以及P3P隐私策略中存在的语义冲突,提出了一种支持冲突检测的data-recipient结构的P3P形式化语义,并用OWL(Web ontology language)本体对该语义进行了描述,阐述了语义冲突的检测方法,并通过实验验证了该方法的可行性。展开更多
The PnP problem is a widely used technique for pose determination in computer vision community,and finding out geometric conditions of multiple solutions is the ultimate and most desirable goal of the multi-solution a...The PnP problem is a widely used technique for pose determination in computer vision community,and finding out geometric conditions of multiple solutions is the ultimate and most desirable goal of the multi-solution analysis,which is also a key research issue of the problem.In this paper,we prove that given 3 control points,if the camera's optical center lies on the so-called“danger cylinder”and is enough far from the supporting plane of control points,the corresponding P3P problem must have 3 positive solutions.This result can bring some new insights into a better understanding of the multi-solution problem.For example,it is shown in the literature that the solution of the P3P problem is instable if the optical center lies on this danger cylinder,we think such occurrence of triple-solution is the primary source of this instability.展开更多
The incidence rate of kidney diseases in China has always remained high.At present,the clinical treatment mainly focuses on symptomatic treatment to delay the progression of the disease,and there is a lack of economic...The incidence rate of kidney diseases in China has always remained high.At present,the clinical treatment mainly focuses on symptomatic treatment to delay the progression of the disease,and there is a lack of economical and effective treatment methods.MicroRNA plays an important regulatory role in the occurrence and development of diseases.This study aims to explore the role and regulatory mechanism of miR⁃142a⁃3p in adriamycin(ADR)⁃induced renal tubular epithelial cell(TCMK⁃1)injury,with a focus on its potential as a therapeutic target for ADR nephropathy.First,cell viability was assessed using the CCK⁃8 kit,and a mouse renal tubular epithelial cell model induced by ADR was established.Subsequently,alterations in miR⁃142a⁃3p and its target gene ATG16L1 mRNA levels were quantified using RT⁃qPCR.Western blotting was used to detect the protein levels of autophagy marker proteins and pyroptosis marker proteins.Monodansylcadaverin(MDC)staining was performed and the autophagy of cells was detected by flow cytometry.The results showed that the relative expression of miR⁃142a⁃3p in TCMK⁃1 cells induced by ADR was increased and the relative expression of its target gene ATG16L1 was decreased(P<0.0001).Western blotting results showed that the levels of p62(P<0.001)and pyroptosis⁃related proteins(P<0.001)were increased,while the protein levels of autophagy⁃related proteins were decreased(P<0.05).The flow cytometry results showed that there was no difference in the mean fluorescence intensity of autoph⁃agosomes between the ADR group and the autophagosome inhibitor group(3⁃MA group)(P>0.05),indicating that after ADR induction,cell autophagy was inhibited and pyroptosis was enhanced.When the expression of miR⁃142a⁃3p was inhibited by transfecting miR⁃142a⁃3p inhibitor,the relative expression level of the target gene ATG16L1 was restored(P<0.001).Western blotting showed that the protein level of p62(P<0.01)and pyroptosis⁃related proteins(P<0.01)were decreased,and the protein level of autophagy⁃related proteins was restored(P<0.001).Flow cytometry results further indicated that cell autophagy was restored(P<0.0001).In conclusion,ADR targets ATG16L1 through miR⁃142a⁃3p to reduce the autophagy level of TCMK⁃1,and simultaneously activates GSDMD⁃mediated pyroptosis.展开更多
基金Supported by the National Natural Science Foundation of China under Grant Nos.60375006,60475009(国家自然科学基金)the Research Foundation of North China University of Technology(北方工业大学校科研基金)
基金Supported by"973"Program(2002CB312104)National Natural Science Foundation of P.R.China(60375006)the Research Foundation of North China Unversity of Technology University
文摘The PnP problem is a widely used technique for pose determination in computer vision community,and finding out geometric conditions of multiple solutions is the ultimate and most desirable goal of the multi-solution analysis,which is also a key research issue of the problem.In this paper,we prove that given 3 control points,if the camera's optical center lies on the so-called“danger cylinder”and is enough far from the supporting plane of control points,the corresponding P3P problem must have 3 positive solutions.This result can bring some new insights into a better understanding of the multi-solution problem.For example,it is shown in the literature that the solution of the P3P problem is instable if the optical center lies on this danger cylinder,we think such occurrence of triple-solution is the primary source of this instability.
文摘The incidence rate of kidney diseases in China has always remained high.At present,the clinical treatment mainly focuses on symptomatic treatment to delay the progression of the disease,and there is a lack of economical and effective treatment methods.MicroRNA plays an important regulatory role in the occurrence and development of diseases.This study aims to explore the role and regulatory mechanism of miR⁃142a⁃3p in adriamycin(ADR)⁃induced renal tubular epithelial cell(TCMK⁃1)injury,with a focus on its potential as a therapeutic target for ADR nephropathy.First,cell viability was assessed using the CCK⁃8 kit,and a mouse renal tubular epithelial cell model induced by ADR was established.Subsequently,alterations in miR⁃142a⁃3p and its target gene ATG16L1 mRNA levels were quantified using RT⁃qPCR.Western blotting was used to detect the protein levels of autophagy marker proteins and pyroptosis marker proteins.Monodansylcadaverin(MDC)staining was performed and the autophagy of cells was detected by flow cytometry.The results showed that the relative expression of miR⁃142a⁃3p in TCMK⁃1 cells induced by ADR was increased and the relative expression of its target gene ATG16L1 was decreased(P<0.0001).Western blotting results showed that the levels of p62(P<0.001)and pyroptosis⁃related proteins(P<0.001)were increased,while the protein levels of autophagy⁃related proteins were decreased(P<0.05).The flow cytometry results showed that there was no difference in the mean fluorescence intensity of autoph⁃agosomes between the ADR group and the autophagosome inhibitor group(3⁃MA group)(P>0.05),indicating that after ADR induction,cell autophagy was inhibited and pyroptosis was enhanced.When the expression of miR⁃142a⁃3p was inhibited by transfecting miR⁃142a⁃3p inhibitor,the relative expression level of the target gene ATG16L1 was restored(P<0.001).Western blotting showed that the protein level of p62(P<0.01)and pyroptosis⁃related proteins(P<0.01)were decreased,and the protein level of autophagy⁃related proteins was restored(P<0.001).Flow cytometry results further indicated that cell autophagy was restored(P<0.0001).In conclusion,ADR targets ATG16L1 through miR⁃142a⁃3p to reduce the autophagy level of TCMK⁃1,and simultaneously activates GSDMD⁃mediated pyroptosis.
