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小花棘豆(Oxytripis glabra DC)毒素基因的cDNA克隆及序列测定 被引量:1
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作者 张桦 王希东 +3 位作者 石庆华 张强 许健 喻梅辉 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2005年第2期176-179,共4页
在完成小花棘豆毒素 95 %氨基酸序列的基础上 ,根椐已知的氨基酸序列 ,设计合成了特异简并引物 .以小花棘豆总RNA为模板 ,逆转录合成cDNA第一链 ,用置换法合成双链cDNA .用特异引物对此双链cDNA进行PCR扩增 ,将扩增后的目的基因与用Sma... 在完成小花棘豆毒素 95 %氨基酸序列的基础上 ,根椐已知的氨基酸序列 ,设计合成了特异简并引物 .以小花棘豆总RNA为模板 ,逆转录合成cDNA第一链 ,用置换法合成双链cDNA .用特异引物对此双链cDNA进行PCR扩增 ,将扩增后的目的基因与用SmaⅠ酶切的质粒pUC 18连接 ,转化大肠杆菌JM10 7.筛选阳性克隆进行序列分析 ,获得了OXY基因的全部序列 .经测序后测得基因序列与原氨基酸序列对照完全一致 .GenBnak数据检索说明 ,OXY基因编码序列确定是一个从未报道的序列 .此研究结果对该毒素的应用研究奠定了基础 . 展开更多
关键词 小花棘豆毒素(oxy) CDNA克隆 序列分析
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D-Ser2-Oxyntomodulin改善Aβ31-35所致小鼠昼夜节律紊乱
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作者 赵今 原媛 +3 位作者 张蕊 王昌图 王丽 王晓晖 《神经解剖学杂志》 CAS CSCD 北大核心 2018年第3期305-312,共8页
目的:观察胃泌酸调节素类似物D-Ser2-Oxyntomodulin(Oxy)是否可以改善β淀粉样蛋白31-35(Aβ31-35)所致的小鼠昼夜节律紊乱,探讨Oxy改善Aβ31-35所致昼夜节律紊乱的细胞分子机制。方法:(1)选取6~8周龄C57BL/6雄性小鼠进行跑轮行为学实验... 目的:观察胃泌酸调节素类似物D-Ser2-Oxyntomodulin(Oxy)是否可以改善β淀粉样蛋白31-35(Aβ31-35)所致的小鼠昼夜节律紊乱,探讨Oxy改善Aβ31-35所致昼夜节律紊乱的细胞分子机制。方法:(1)选取6~8周龄C57BL/6雄性小鼠进行跑轮行为学实验,分析Oxy改善Aβ31-35所致小鼠昼夜节律紊乱的作用。(2)选取小鼠海马HT22神经细胞,CCK8法检测细胞存活率,Real-time PCR检测不同时间点钟基因Bmal1和Per2 mRNA的变化趋势,Western Blot分别检测CT20时Bmal1蛋白和CT16时Per2蛋白的表达情况。结果:(1)Aβ31-35引起小鼠昼夜节律紊乱,与对照组相比,自由运转周期显著延长。Oxy预处理再给予Aβ31-35后小鼠的昼夜节律紊乱情况有所改善,与Aβ单独给予相比,自由运转周期显著降低。(2)Aβ31-35引起HT22细胞存活率显著降低,Oxy预处理后有效逆转Aβ31-35诱导的细胞存活率下降,且具有剂量依赖性。(3)经Aβ31-35处理后,HT22细胞的Bmal1和Per2 mRNA水平分别在CT20和CT16较对照组明显降低;而Oxy预处理组Bmal1和Per2的异常表达均得到显著改善。(4)与对照组相比,Aβ31-35组CT20时Bmal1和CT16时Per2蛋白水平显著降低;而与Aβ31-35组相比,Oxy预处理组Bmal1和Per2蛋白水平有所升高。结论:Oxy可能通过调整Bmal1和Per2的表达改善Aβ31-35所致C57BL/6小鼠昼夜节律紊乱。 展开更多
关键词 昼夜节律 oxy Aβ31-35 钟基因/蛋白Bmal1 PER2 小鼠
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Thermo economic evaluation of oxy fuel combustion cycle in Kazeroon power plant considering enhanced oil recovery revenues 被引量:1
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作者 Ehsan Torabnejad Ramin Haghighi-Khoshkhoo Niloufar Sarabchi 《Journal of Central South University》 SCIE EI CAS 2014年第3期1025-1033,共9页
Oxy fuel combustion and conventional cycle(currently working cycle) in Kazeroon plant are modeled using commercial thermodynamic modeling software. Economic evaluation of the two models regarding the resources of tran... Oxy fuel combustion and conventional cycle(currently working cycle) in Kazeroon plant are modeled using commercial thermodynamic modeling software. Economic evaluation of the two models regarding the resources of transport and injection of carbon dioxide into oil fields at Gachsaran for enhanced oil recovery in the various oil price indices is conducted and indices net present value(NPV) and internal rate of return on investment(IRR) are calculated. The results of the two models reveal that gross efficiency of the oxy fuel cycle is more than reference cycle(62% compared to 49.03%), but the net efficiency is less(41.85% compared to 47.92%) because of the high-energy consumption of the components, particularly air separation unit(ASU) in the oxy fuel cycle. In this model, pure carbon dioxide with pressure of 20×105 Pa and purity of 96.84% was captured. NOX emissions also decrease by 4289.7 tons per year due to separation of nitrogen in ASU. In this model, none of the components of oxy fuel cycle is a major engineering challenge. With increasing oil price, economic justification of oxy fuel combustion model increases. With the price of oil at $ 80 per barrel in mind and $ 31 per ton fines for emissions of carbon dioxide in the atmosphere, IRR is the same for both models. 展开更多
关键词 oxy fuel combustion: C02 capture combined cycle enhanced oil recovery NOx reduction
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兰州沙尘总悬浮微粒中有机污染物的变化及来源 被引量:4
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作者 奚晓霞 白艳 +3 位作者 孟仟祥 王鑫 佘峰 吴方堃 《兰州大学学报(自然科学版)》 CAS CSCD 北大核心 2009年第2期36-42,共7页
比较了2006年春季沙尘天气和非沙尘天气吸附到TSP样品中增塑剂、正构烷烃、多环芳烃(PAHs)、甾烷和藿烷类等有机污染组分特征.显示兰州TSP中增塑剂等的工业污染相当严重,煤和生物质燃烧排放以及交通排放较高.非沙尘天气时,污染物主体主... 比较了2006年春季沙尘天气和非沙尘天气吸附到TSP样品中增塑剂、正构烷烃、多环芳烃(PAHs)、甾烷和藿烷类等有机污染组分特征.显示兰州TSP中增塑剂等的工业污染相当严重,煤和生物质燃烧排放以及交通排放较高.非沙尘天气时,污染物主体主要来源于交通排放藿烷和化石燃料燃烧产物释放(煤和生物质燃烧排放为主)的PAHs;而沙尘天气时,虽然兰州地区气体颗粒物急剧增多,但由于有机污染物被沙尘暴带到东部地区,这些有机物质量分数都明显降低.在非沙尘天气,可能是由于沙尘暴源区和沿途土壤高等植物蜡质的风成输入,生物来源的正构烷烃贡献相对增加,其中5个oxy-PAHs和邻苯二甲酸二异壬酯在大气环境研究中很少报道. 展开更多
关键词 总悬浮微粒 PAHS oxy—PAHs 甾烷和藿烷 污染源 兰州
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Identifying the optimal HVOF spray parameters to attain minimum porosity and maximum hardness in iron based amorphous metallic coatings 被引量:12
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作者 S.Vignesh K.Shanmugam +1 位作者 V.Balasubramanian K.Sridhar 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2017年第2期101-110,共10页
Flow based Erosion e corrosion problems are very common in fluid handling equipments such as propellers, impellers, pumps in warships, submarine. Though there are many coating materials available to combat erosionecor... Flow based Erosion e corrosion problems are very common in fluid handling equipments such as propellers, impellers, pumps in warships, submarine. Though there are many coating materials available to combat erosionecorrosion damage in the above components, iron based amorphous coatings are considered to be more effective to combat erosionecorrosion problems. High velocity oxy-fuel(HVOF)spray process is considered to be a better process to coat the iron based amorphous powders. In this investigation, iron based amorphous metallic coating was developed on 316 stainless steel substrate using HVOF spray technique. Empirical relationships were developed to predict the porosity and micro hardness of iron based amorphous coating incorporating HVOF spray parameters such as oxygen flow rate, fuel flow rate, powder feed rate, carrier gas flow rate, and spray distance. Response surface methodology(RSM) was used to identify the optimal HVOF spray parameters to attain coating with minimum porosity and maximum hardness. 展开更多
关键词 High velocity oxy fuel SPRAY IRON BASED AMORPHOUS metallic coating Micro-hardness POROSITY
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国外从石榴中提取功能物质
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作者 宋铁军 《农产品加工》 2004年第5期15-15,共1页
关键词 石榴 提取 功能物质 oxy Phyte” RFI食物配料公司 抗氧化能力 鞣质酸
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