Oncorhynchus mykiss is delicious and contains abundant flavor substances.However,few studies focused on umami peptides of O.mykiss.In the current work,umami peptides derived from O.mykiss were identified using virtual...Oncorhynchus mykiss is delicious and contains abundant flavor substances.However,few studies focused on umami peptides of O.mykiss.In the current work,umami peptides derived from O.mykiss were identified using virtual screening,molecular docking,and electronic tongue analysis.First,the O.mykiss protein was hydrolyzed using the PeptideCutter online enzymolysis program.Subsequently,water-soluble and toxicity screening were performed by Innovagen and ToxinPred software,respectively.The potential peptides were docked with umami receptor T1R1/T1R3.Furthermore,taste properties of potential peptides were validated by electronic tongue.Docking results suggested that the three tetrapeptide EANK,EEAK,and EMQK could enter the binding pocket in the T1R1 cavity,wherein Arg151,Asp147,Gln52,and Arg277 may play key roles in the production of umami taste.Electronic tongue results showed that the umami value of EANK,EEAK,and EMQK were stronger than monosodium glutamate.This work provides a new insight for the screening of umami peptides in O.mykiss.展开更多
为探究钙调控因子在硬骨鱼类钙代谢过程中发挥的作用,本研究选取细胞内Ca^(2+)信号传导的关键调控因子-钙调素(Calmodulin,CaM)为研究对象,通过对虹鳟(Oncorhynchus mykiss)幼鱼腹腔注射氯化卡咪唑铵(Calmidazolium chloride,CMZ)抑制Ca...为探究钙调控因子在硬骨鱼类钙代谢过程中发挥的作用,本研究选取细胞内Ca^(2+)信号传导的关键调控因子-钙调素(Calmodulin,CaM)为研究对象,通过对虹鳟(Oncorhynchus mykiss)幼鱼腹腔注射氯化卡咪唑铵(Calmidazolium chloride,CMZ)抑制CaM活性,并在注射后6 h采集实验组及对照组(腹腔注射同体积生理盐水)虹鳟鳞片组织,利用转录组测序技术分析实验组mRNA的表达变化。结果显示:转录组测序共获得376.31 M Raw reads,Q30碱基百分比均在93.26%以上;实验组鳞片组织中共筛选出3730个差异表达基因(DEGs),其中1227个基因表达上调,2503个基因表达下调。GO注释结果显示,上述DEGs主要富集到细胞过程、生物调控、细胞器、结合和生物过程调控等功能;KEGG通路富集分析结果显示,DEGs显著富集的前3条通路依次为核糖体通路、Rap1信号通路和轴突导向通路,此外,DEGs还显著富集于破骨细胞分化、TGF-beta等与骨骼发育、钙代谢等密切相关的信号通路。本研究所获得的差异表达基因信息及其功能注释和通路富集结果可为阐明鱼类钙代谢的调控机理提供新素材。展开更多
基金supported by The National Key R&D Program of China (2019YFD0901702)
文摘Oncorhynchus mykiss is delicious and contains abundant flavor substances.However,few studies focused on umami peptides of O.mykiss.In the current work,umami peptides derived from O.mykiss were identified using virtual screening,molecular docking,and electronic tongue analysis.First,the O.mykiss protein was hydrolyzed using the PeptideCutter online enzymolysis program.Subsequently,water-soluble and toxicity screening were performed by Innovagen and ToxinPred software,respectively.The potential peptides were docked with umami receptor T1R1/T1R3.Furthermore,taste properties of potential peptides were validated by electronic tongue.Docking results suggested that the three tetrapeptide EANK,EEAK,and EMQK could enter the binding pocket in the T1R1 cavity,wherein Arg151,Asp147,Gln52,and Arg277 may play key roles in the production of umami taste.Electronic tongue results showed that the umami value of EANK,EEAK,and EMQK were stronger than monosodium glutamate.This work provides a new insight for the screening of umami peptides in O.mykiss.
文摘为探究钙调控因子在硬骨鱼类钙代谢过程中发挥的作用,本研究选取细胞内Ca^(2+)信号传导的关键调控因子-钙调素(Calmodulin,CaM)为研究对象,通过对虹鳟(Oncorhynchus mykiss)幼鱼腹腔注射氯化卡咪唑铵(Calmidazolium chloride,CMZ)抑制CaM活性,并在注射后6 h采集实验组及对照组(腹腔注射同体积生理盐水)虹鳟鳞片组织,利用转录组测序技术分析实验组mRNA的表达变化。结果显示:转录组测序共获得376.31 M Raw reads,Q30碱基百分比均在93.26%以上;实验组鳞片组织中共筛选出3730个差异表达基因(DEGs),其中1227个基因表达上调,2503个基因表达下调。GO注释结果显示,上述DEGs主要富集到细胞过程、生物调控、细胞器、结合和生物过程调控等功能;KEGG通路富集分析结果显示,DEGs显著富集的前3条通路依次为核糖体通路、Rap1信号通路和轴突导向通路,此外,DEGs还显著富集于破骨细胞分化、TGF-beta等与骨骼发育、钙代谢等密切相关的信号通路。本研究所获得的差异表达基因信息及其功能注释和通路富集结果可为阐明鱼类钙代谢的调控机理提供新素材。
