In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Amo...In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain.展开更多
Background: Unresectable pancreatic cancer haspoor prognosis and no effective treatment. Somatic genetherapy is currently being investigated as a possibletreatment for malignancies. One of the most promisingstrategies...Background: Unresectable pancreatic cancer haspoor prognosis and no effective treatment. Somatic genetherapy is currently being investigated as a possibletreatment for malignancies. One of the most promisingstrategies is the introduction of herpes simplex virus type-1 thymidine kinase (HSV-tk) in the cells, followed bytreatment with the antiviral drug ganciclovir (GCV).展开更多
Objectives:To isolate tumor metastaticsuppressing genes or relating human DNA sequencesand to study the molecular biological regulatingmechanism of tumor metastasis. Methods: HumanDNA fragments were amplified by Inter...Objectives:To isolate tumor metastaticsuppressing genes or relating human DNA sequencesand to study the molecular biological regulatingmechanism of tumor metastasis. Methods: HumanDNA fragments were amplified by Inter Alu PCRtechnique from normal human genomic DNAtransfected mouse tumor cell clones of which themetastatic phenotype had been suppressed. Thehuman origin of the amplified DNA was furtherconfirmed by PCR in situ hybridization. One展开更多
In order to investigate the effects of cytokine fusedgene transfection on tumor cell modification, threeretroviral vectors for human interleukin 6 (IL-6),interleukin 2 (IL-2) and, IL-6/IL-2 fusion gene wereconstructed...In order to investigate the effects of cytokine fusedgene transfection on tumor cell modification, threeretroviral vectors for human interleukin 6 (IL-6),interleukin 2 (IL-2) and, IL-6/IL-2 fusion gene wereconstructed. The retroviral vectors were introduced intomurine B16 melanoma cell line respectively and theeffects of single or fused cytokine gene on tumor cellbiology in vitro and in vivo were examined. In vivo, thethree cytokine gene-modified B16 cells all showeddecreased tumorigenicity and metastatic potentials,however, the reduction in tumorigenicity and展开更多
Despite significant progress in cancer research during the past decades,yet there are no major breakthroughs that can be translated into major benefits for the general public in terms of treatment or therapy for the c...Despite significant progress in cancer research during the past decades,yet there are no major breakthroughs that can be translated into major benefits for the general public in terms of treatment or therapy for the complex neoplastic diseases,especially for the malignant solid tumors.This depressing but indisputable fact leads to a call for new ideas to target tumor metastasis by editors of Nature Medicine<sup>[1]</sup>.The real problems are that the fundamental issues of transformation and malignancy in vivo are poorly understood.In a recent review on cancer,展开更多
Introduction Hematogenous metastasis is the mainly leading cause of death in breast carcinoma patients.A better understanding of the underlying molecular and cellular mechanisms is crucial for the development of effec...Introduction Hematogenous metastasis is the mainly leading cause of death in breast carcinoma patients.A better understanding of the underlying molecular and cellular mechanisms is crucial for the development of effective treatment for metastatic breast cancer<sup>[</sup>1].It has been well established that cell adhesion and invasion is mediated by a variety of transmembrane proteins,including integrins,cadherins,selectins,and intercellular adhesion molecules.Among these adhesion molecules,the integrins and their downstream signaling pathways have been extensively studied<sup>[2]</sup>.On the other hand,the specific events determining tumor cell interactions with endo-展开更多
文摘In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain.
文摘Background: Unresectable pancreatic cancer haspoor prognosis and no effective treatment. Somatic genetherapy is currently being investigated as a possibletreatment for malignancies. One of the most promisingstrategies is the introduction of herpes simplex virus type-1 thymidine kinase (HSV-tk) in the cells, followed bytreatment with the antiviral drug ganciclovir (GCV).
文摘Objectives:To isolate tumor metastaticsuppressing genes or relating human DNA sequencesand to study the molecular biological regulatingmechanism of tumor metastasis. Methods: HumanDNA fragments were amplified by Inter Alu PCRtechnique from normal human genomic DNAtransfected mouse tumor cell clones of which themetastatic phenotype had been suppressed. Thehuman origin of the amplified DNA was furtherconfirmed by PCR in situ hybridization. One
文摘In order to investigate the effects of cytokine fusedgene transfection on tumor cell modification, threeretroviral vectors for human interleukin 6 (IL-6),interleukin 2 (IL-2) and, IL-6/IL-2 fusion gene wereconstructed. The retroviral vectors were introduced intomurine B16 melanoma cell line respectively and theeffects of single or fused cytokine gene on tumor cellbiology in vitro and in vivo were examined. In vivo, thethree cytokine gene-modified B16 cells all showeddecreased tumorigenicity and metastatic potentials,however, the reduction in tumorigenicity and
基金supported by the funds from Huazhong University of Science and TechnologyUS NIH grant GM072744
文摘Despite significant progress in cancer research during the past decades,yet there are no major breakthroughs that can be translated into major benefits for the general public in terms of treatment or therapy for the complex neoplastic diseases,especially for the malignant solid tumors.This depressing but indisputable fact leads to a call for new ideas to target tumor metastasis by editors of Nature Medicine<sup>[1]</sup>.The real problems are that the fundamental issues of transformation and malignancy in vivo are poorly understood.In a recent review on cancer,
基金The financial supports provided,in whole or in part,by the National Natural Science Foundation of China(11272083)the New Century Excellent Talents Program in Chinese Universities(NCET09-0263)
文摘Introduction Hematogenous metastasis is the mainly leading cause of death in breast carcinoma patients.A better understanding of the underlying molecular and cellular mechanisms is crucial for the development of effective treatment for metastatic breast cancer<sup>[</sup>1].It has been well established that cell adhesion and invasion is mediated by a variety of transmembrane proteins,including integrins,cadherins,selectins,and intercellular adhesion molecules.Among these adhesion molecules,the integrins and their downstream signaling pathways have been extensively studied<sup>[2]</sup>.On the other hand,the specific events determining tumor cell interactions with endo-
