A study was conducted to analyze the deformation mechanism of strongly weathered quartz schist in the Daliangshan Tunnel,located in the western Transverse Mountain area.A large deformation problem was experienced duri...A study was conducted to analyze the deformation mechanism of strongly weathered quartz schist in the Daliangshan Tunnel,located in the western Transverse Mountain area.A large deformation problem was experienced during the tunnel construction.To mitigate this problem,a support system was designed incorporating negative Poisson ratio(NPR)anchor cables with negative Poisson ratio effect.Physical model experiments,field experiments,and numerical simulation experiments were conducted to investigate the compensation mechanical behavior of NPR anchor cables.The large deformations of soft rocks in the Daliangshan Tunnel are caused by a high ground stress,a high degree of joint fracture development,and a high degree of surrounding rock fragmentation.A compensation mechanics support system combining long and short NPR anchor cables was suggested to provide sufficient counter-support force(approximately 350 kN)for the surrounding rock inside the tunnel.Comparing the NPR anchor cable support system with the original support system used in the Daliangshan tunnel showed that an NPR anchor cable support system,combining cables of 6.3 m and 10.3 m in length,effectively prevented convergence of surrounding rock deformation,and the integrated settlement convergence value remained below 300 mm.This study provides an effective scientific basis for resolving large deformation problems in deeply buried soft rocks in western transverse mountain areas.展开更多
A new mechanics model, which reveals additional longitudinal force transmission between the continuously welded rails and the bridges, is established on the fact that the influence of the mutual relative displacement ...A new mechanics model, which reveals additional longitudinal force transmission between the continuously welded rails and the bridges, is established on the fact that the influence of the mutual relative displacement (among) the rail, the sleeper and the beam is taken into account. An example is presented and numerical results are compared. The results show that the additional longitudinal forces calculated with the new model are less than those of the previous, especially in the case of the flexible pier bridges. The new model is also suitable for the analysis of the additional longitudinal force transmission between rails and bridges of ballastless track with small resistance fasteners without taking the sleeper displacement into account, and compared with the ballast bridges, the ballastless bridges have a much stronger additional longitudinal force transmission between the continuously welded rails and the bridges.展开更多
The mesoscopic failure mechanism and the macro-mechanical characteristics of soil-rock mixture(S-RM) under external load are largely controlled by S-RM's meso-structural features. The objective of this work is to ...The mesoscopic failure mechanism and the macro-mechanical characteristics of soil-rock mixture(S-RM) under external load are largely controlled by S-RM's meso-structural features. The objective of this work is to improve the three-dimensional technology for the generation of the random meso-structural models of S-RM, for randomly generating irregular rock blocks in S-RM with different shapes, sizes, and distributions according to the characteristics of the rock blocks' size distribution. Based on the new improved technology, a software system named as R-SRM3 D for generation and visualization of S-RM is developed. Using R-SRM3 D, a three-dimensional meso-structural model of S-RM is generated and used to study the meso-mechanical behavior through a series of true-triaxial numerical tests. From the numerical tests, the following conclusions are obtained. The meso-stress field of S-RM is influenced by the distribution of the internal rock blocks, and the macro-mechanical characteristics of S-RM are anisotropic in 3D; the intermediate principal stress and the soil-rock interface properties have significant influence on the macro strength of S-RM.展开更多
Soil-rock mixture(S-RM)is a widely distributed geotechnical medium composed of "soil" and "rock block" different both in size and strength. Internal rock blocks form special and variable meso-struc...Soil-rock mixture(S-RM)is a widely distributed geotechnical medium composed of "soil" and "rock block" different both in size and strength. Internal rock blocks form special and variable meso-structural characteristics of S-RM. The objective of this work was to study the control mechanism of meso-structural characteristics on mechanical properties of S-RM. For S-RM containing randomly generated polygonal rock blocks, a series of biaxial tests based on DEM were conducted. On the basis of research on the effects of rock blocks' breakability and sample lateral boundary type(rigid, flexible) on macroscopic mechanical behavior of S-RM, an expanded Mohr-Coulomb criterion in power function form was proposed to represent the strength envelop. At the mesoscopic level, the variations of meso-structure such as rotation of rock block, and the formation mechanism and evolution process of the shear band during tests were investigated. The results show that for S-RM with a high content of rock block, translation, rotating and breakage of rock blocks have crucial effects on mechanical behavior of S-RM. The formation and location of the shear band inside S-RM sample are also controlled by breakability and arrangement of rock blocks.展开更多
This paper deals with the mechanics problem of dynamic walking of anthropomorphic biped robots. Through analysing the mechanics system of this kind of robots in detail, the motion constraint equations are established,...This paper deals with the mechanics problem of dynamic walking of anthropomorphic biped robots. Through analysing the mechanics system of this kind of robots in detail, the motion constraint equations are established, three mechanics laws describing the r展开更多
Land subsidence hazard,which is caused by drawing groundwater,has been a problem of the world.In order to control the development of land subsidence,exploited horizon of groundwater was adjusted from the shallow layer...Land subsidence hazard,which is caused by drawing groundwater,has been a problem of the world.In order to control the development of land subsidence,exploited horizon of groundwater was adjusted from the shallow layer to the deep layer in the past years.But the deformation mechanics study of the aquitard in different depth is not enough.There were essential distinctions between the shallow aquitard and the deep aquitard in engineering geology properties.The paper used the different depth aquitard of Tianjin city as an example to study the展开更多
Cancer stem cells(CSCs)are the driving force for sustainable tumor growth and metastasis and responsible for drug resistance and cancer relapse.Nanoparticle-based drug delivery has been demonstrated to be effective in...Cancer stem cells(CSCs)are the driving force for sustainable tumor growth and metastasis and responsible for drug resistance and cancer relapse.Nanoparticle-based drug delivery has been demonstrated to be effective in combating tumor growth.However,it has been challenging to selectively eliminate CSCs due to the lack of a general signature for a spectrum of cancers.It is known that CSCs from various types of cancer show lower stiffness compared to non-CSCs.It remains unclear whether low stiffness in CSCs influences cellular uptake in nanoparticle-based drug delivery and thus the chemotherapy efficacy.Graphene quantum dot(GQD)is emerging as a promising carrier material in delivering anti-cancer drugs.We found that breast CSCs were softer than conventional cancer cells,which were further softer compared to healthy breast tissue cells.Importantly,soft CSCs uptook more GQD than conventional cancer cells,while stiff breast cancer cells with relatively low stiffness uptook more GQD than healthy breast cells.Softening cells by pharmacologically inhibiting actomyosin activity using either siRNA or actomyosin inhibitors significantly enhanced the cellular uptake of GQD in breast cancer cells but not CSCs,while stiffening cells by activating actomyosin using CA-MLCK/ROCK or actomyosin activators considerably suppressed the nanoparticle uptake in both cancer cells and CSCs.GQD could specifically target CSC because of low cell stiffness of CSC in breast cancer cell line MCF-7 and MDA-MB-231.Further regulating cell stiffness reflected that decreasing breast cancer cell stiffness by inhibiting actomyosin activity using blebbistatin could promote GQD uptake.Vice versa,stiffening cancer cell by activating actomyosin decreased GQD uptake.The attachment of anti-cancer drug doxorubicin did not alter the trend of GQD uptake in neither soften nor stiffen cancer cells.Actomyosin activity regulates cellular uptake ofGQD might through clathrin and caveolin-mediated endocytosis.Cancer cells are softer than normal cells from the same organ,CSC are softer than non-CSC.Thus we further confirmed that the GQD uptake of normal breast cell line MCF-10 is less than breast cancer cell line MCF-7 and MDA-MB-231.Suggesting the clinical significance that using GQD as drug carrier targeting softer cells could reduce side effects to normal tissue cells.Since CSC are softer than non-CSC,GQD could decreased the percentage of CSC in whole cancer cell population by targeting softer cells.These results suggesting that it would be possible to target cancer cells and CSC by targeting from a perspective of cell mechanical difference.High uptake of nanoparticles in soft cancer cells could not be explained by their differential membrane potentials.Mechanistically,low cell mechanics or inhibiting actomyosin activity activated both clathrin and caveolin-mediated endocytosis signaling pathways,while high cell mechanics or activating actomyosin suppressed these signalings.Pharmacologically inhibiting clathrin or caveolin-mediated endocytosis signaling significantly decreased GQD uptake in CSCs and in conventional breast cancer cells when actomyosin was suppressed.Further,GQD conjugated with doxorubicin could be specifically delivered into CSCs with low stiffness and eliminated more CSCs in the presence of both CSCs and non-CSCs.Taken together,these data reveal the regulatory role of cell mechanics in cellular uptake of nanoparticles and demonstrate that GQD can be utilized to specifically eliminate CSCs,which have important implications in nanoparticle-based drug delivery for cancer therapy.展开更多
Metastasis is the main cause of cancer death,and tumor cells mainly disseminate to the distal organs through the blood circulation,in which they experience considerable levels of fluid shear stress.CTCs are heterogene...Metastasis is the main cause of cancer death,and tumor cells mainly disseminate to the distal organs through the blood circulation,in which they experience considerable levels of fluid shear stress.CTCs are heterogeneous with diverse subpopulations of distinct genotypes and phenotypes and the frequency of CTCs is correlated with poor prognosis and overall survival in cancer patients.Less than 0.01%of them may eventually generate metastatic tumors in secondary sites,indicating the inefficiency of metastasis.Nevertheless,metastasis accounts for over 90%of cancer-related deaths,suggesting that a subpopulation of CTCs are able to survive the metastatic process and form metastases.To target metastasis,it is thus essential to understand the roles of various factors during dissemination in the survival and functions of CTCs.However,the effects of hemodynamic shear stress on biophysical properties and functions of CTCs in suspension are not fully understood.This study was to investigate the effect of hemodynamic shear stress on the survival and anti-chemotherapy ability of suspended circulating tumor cells during metastasis,and the effect of actomyosin activity on this regulation.In this study,we developed a circulatory system to generate physiologic levels of hemodynamic shear stress,which mimicked certain important aspects of the CTC microenvironment in blood circulation.The survival of tumor cells in suspension,as a model for real CTCs,under different shear stress and circulation duration was examined.We found that the majority of breast tumor cells s in suspension can be eliminated by hemodynamic shear stress.The surviving cells exhibit unique biophysical properties,including significantly retarded cell adhesion,mesenchymal-like cell morphology,and reduced F-actin expression and cellular stiffness.Cancer stem cells which has been reported in other papers have lower stiffness compared with conventional tumor cells showed significantly higher survival in blood flow.Importantly,low actomyosin activity promotes the survival of CTCs in blood shear flow while high actomyosin activity inhibits tumor cells surviving shear stress treatment.These findings might be explained by the up-and down-regulation of the anti-apoptosis genes.Soft surviving tumor cells held survival advantages in shear flow and higher resistance to chemotherapy.Metastasis is closely linked with chemoresistance.However,the underlying mechanisms have not been fully understood,in particular,the roles of hemodynamic shear stress and actomyosin-dependent cell mechanics in drug resistance of CTCs remain unclear.Inhibiting actomyosin activity in suspended tumor cells enhanced chemoresistance,while activating actomyosin suppressed this ability.These findings might be associated with the corresponding changes in multidrug resistance related genes.Our study unveils the regulatory roles of actomyosin in the survival and drug resistance of circulating tumor cells in hemodynamic shear flow,which imply the importance of fluid shear stress and actomyosin activity in tumor metastasis.Our findings reveal a new mechanism by which circulating tumor cells are able to survive hemodynamic shear stress and chemotherapy and may offer a new potential strategy to target circulating tumor cells in shear flow and combat chemoresistance through actomyosin.展开更多
Recently, this study group established “the map of MOHO\|surface bathymetric line in Chinese and Near Region" on the basis of latest survey and study of the crustal depth, the preliminary result shows that the r...Recently, this study group established “the map of MOHO\|surface bathymetric line in Chinese and Near Region" on the basis of latest survey and study of the crustal depth, the preliminary result shows that the regular meridional and latitudinal upwarping and downwarping structural pattern of MOHO\|surface bathymetric line among Eurasian plate and Pacific plate and the Indian plate alternately appears, and which is accreted and coupled with basin ridge structure that exist shallow crustal base, continental crust and oceanic crust and others regular upwarping and downwarping net structure system that possessing different block characters and different scales exist together. Among different structure systems, it occurs that ramp downwarping impetus transform structure belts whose trends is characteristic. Nowadays upwarping and downwarping net structure system is basically modeled in Himalayan orogeny period. It is showed that the Earth revolution way has been changed in this period, which leaded to a new Earth dynamics cycle.The pattern of upwarping and downwarping structure among different structure systems or different structure blocks , and the characters of different trends and different scale transform structure belts, reflects the structure movement way and their conversion law, and reveals the Earth centralized dynamics mechanics that is produced by the revolutionary effect under the environments of aster system. This can be clearly reflected by the change of impetus way between Qinghai—Tibet highland structure system and near structure system.展开更多
The rapid development of nanotechnology enables the successful application of target drug delivery,which provides new hope for the clinical examination and treatment.In the whole process of drug delivery,we foundcoupl...The rapid development of nanotechnology enables the successful application of target drug delivery,which provides new hope for the clinical examination and treatment.In the whole process of drug delivery,we foundcouple of mechanics problems existed,including the transportation of nanocarriers,the molecular level target and the cellular uptake of nanoparticles.In recent years,we focused on the investigation of diffusion of drug delivery systems in mucus of gastrointestinal(GI)tract and tumor intestinal.We studied the stiffness effect and geometric effect of nanoparticles in cell internalization,and found that the shape,stiffness and adhesion of nanoparticle-based drug carriers affect their transportation in biological tissues.We revealed the internal mechanism with a theoretical model for the diffusion of nanoparticles in an adhesive/nonadhesive polymer network.These findings shed new light on the design of NP-based drug delivery systems targeted to mucosal and tumor sites that possess a fibrous structure/porous medium.展开更多
This article demonstrates a novel approach for material nonlinear analysis.This analysis procedure eliminates tedious and lengthy step by step incremental and then iterative procedure adopted classically and gives dir...This article demonstrates a novel approach for material nonlinear analysis.This analysis procedure eliminates tedious and lengthy step by step incremental and then iterative procedure adopted classically and gives direct results in the linear as well as in nonlinear range of the material behavior.Use of elastic moduli is eliminated.Instead,stress and strain functions are used as the material input in the analysis procedure.These stress and strain functions are directly derived from the stress-strain behavior of the material by the method of curve fitting.This way,the whole stress-strain diagram is utilized in the analysis which naturally exposes the response of structure when loading is in nonlinear range of the material behavior.It is found that it is an excellent computational procedure adopted so far for material nonlinear analysis which gives very accurate results,easy to adopt and simple in calculations.The method eliminates all types of linearity assumptions in basic derivations of equations and hence,eliminates all types of possibility of errors in the analysis procedure as well.As it is required to know stress distribution in the structural body by proper modelling and structural idealization,the proposed analysis approach can be regarded as stress-based analysis procedure.Basic problems such as uniaxial problem,beam bending,and torsion problems are solved.It is found that approach is very suitable for solving the problems of fracture mechanics.Energy release rate for plate with center crack and double cantilever beam specimen is also evaluated.The approach solves the fracture problem with relative ease in strength of material style calculations.For all problems,results are compared with the classical displacement-based liner theory.展开更多
The relationship between engine mechanics and thermo-dynamics has been investigated by means of numerical simulation.The inherent mismatching between the mechanical behaviors and the thermodynamic process in internal ...The relationship between engine mechanics and thermo-dynamics has been investigated by means of numerical simulation.The inherent mismatching between the mechanical behaviors and the thermodynamic process in internal combustion engine is identified,which is believed to be one of the important limiting factors of energy efficiency for conventional engines available in the current market.An approach for engine efficiency improvement through optimal matching between mechanics and thermodynamics(OMBMT)is proposed.An ideal matching model is defined and the conflicts due to the constraints among the mapping strokes in a 4-stroke engine are analyzed.A novel mechanical model is built for approaching optimal matching among all 4 individual strokes in a 4-stroke spark-ignition engine,which is composed of non-circular gears(NCG)and integrated with conventional slider crank engine mechanism.By means of digital mechanical model and numerical simulation,the matching gains among all 4 strokes are defined and calculated for quantifying the NCG engine efficiency improvement by comparing with a baseline engine.The potentials with the OMBMT implemented and the enhancements made by NCG mechanism for engines in terms of overall engine efficiency are reported.Based on the results achieved,it is recommended that the feasibility studies and the experimental validations should be conducted to verify the engine matching concept and effectiveness of the NCG mechanism engine model proposed,and the engine performance and NCG design parameters should be further optimized.展开更多
The finite element analysis was carried out for a composite vertical axis wind turbine with lift-drag combined starting structures to ensure the structure safety of a vertical axis wind turbine(VAWT).The static and mo...The finite element analysis was carried out for a composite vertical axis wind turbine with lift-drag combined starting structures to ensure the structure safety of a vertical axis wind turbine(VAWT).The static and modal analysis of rotor of a composite vertical axis wind turbine was conducted by using ANSYS software.The relevant contour sketch of stress and deformation was obtained.The analysis was made for static structural mechanics,modal analysis of rotor and the total deformation and vibration profile to evaluate the influence on the working capability of the rotor.The analysis results show that the various structure parameters lie in the safety range of structural mechanics in the relative standards.The analysis showing the design safe to operate the rotor of a vertical axis wind turbine.The methods used in this study can be used as a good reference for the structural mechanics′analysis of VAWTs.展开更多
Focal adhesions(FAs) are large,multiprotein complexs that provides linkers between cytoskeleton to the extracellular matrix(ECM).Cells sense and respond to forces through FAs to regulate a broad range of processes,suc...Focal adhesions(FAs) are large,multiprotein complexs that provides linkers between cytoskeleton to the extracellular matrix(ECM).Cells sense and respond to forces through FAs to regulate a broad range of processes,such as cell growth,migration,differentiation展开更多
Cells sense and respond to forces and extracellular environment through FAs to regulate a broad range of processes, such as cell growth,migration,differentiation and apoptosis. Currently,the underlying mechanisms of t...Cells sense and respond to forces and extracellular environment through FAs to regulate a broad range of processes, such as cell growth,migration,differentiation and apoptosis. Currently,the underlying mechanisms of the force展开更多
The present study deals with the experimental,finite element(FE)and analytical assessment of low ballistic impact response of proposed flexible‘green’composite make use of naturally available jute and rubber as the ...The present study deals with the experimental,finite element(FE)and analytical assessment of low ballistic impact response of proposed flexible‘green’composite make use of naturally available jute and rubber as the constituents of the composite with stacking sequences namely jute/rubber/jute(JRJ),jute/rubber/rubber/jute(JRRJ)and jute/rubber/jute/rubber/jute(JRJRJ).Ballistic impact tests were carried out by firing a conical projectile using a gas gun apparatus at lower range of ballistic impact regime.The ballistic impact response of the proposed flexible composites are assesses based on energy absorption and damage mechanism.Results revealed that inclusion of natural rubber aids in better energy absorption and mitigating the failure of the proposed composite.Among the three different stacking sequences of flexible composites considered,JRJRJ provides better ballistic performance compared to its counterparts.The damage study reveals that the main mechanism of failure involved in flexible composites is matrix tearing as opposed to matrix cracking in stiff composites indicating that the proposed flexible composites are free from catastrophic failure.Results obtained from experimental,FE and analytical approach pertaining to energy absorption and damage mechanism agree well with each other.The proposed flexible composites due to their exhibited energy absorption capabilities and damage mechanism are best suited as claddings for structural application subjected to impact with an aim of protecting the main structural component from being failed catastrophically.展开更多
This study proposed a new and more flexible S-shaped rock damage evolution model from a phenomenological perspective based on an improved Logistic function to describe the characteristics of the rock strain softening ...This study proposed a new and more flexible S-shaped rock damage evolution model from a phenomenological perspective based on an improved Logistic function to describe the characteristics of the rock strain softening and damage process.Simultaneously,it established a constitutive model capable of describing the entire process of rock pre-peak compaction and post-peak strain softening deformation,considering the nonlinear effects of the initial compaction stage of rocks,combined with damage mechanics theory and effective medium theory.In addition,this research verified the rationality of the constructed damage constitutive model using results from uniaxial and conventional triaxial compression tests on Miluo granite,yellow sandstone,mudstone,and glutenite.The results indicate that based on the improved Logistic function,the theoretical damage model accurately describes the entire evolution of damage characteristics during rock compression deformation,from maintenance through gradual onset,accelerated development to deceleration and termination,in a simple and unified expression.At the same time,the constructed constitutive model can accurately simulate the stress-strain process of different rock types under uniaxial and conventional triaxial compression,and the theoretical model curve closely aligns with experimental data.Compared to existing constitutive models,the proposed model has significant advantages.The damage model parameters a,r and β have clear physical meanings and interact competitively,where the three parameters collectively determine the shape of the theoretical stress−strain curve.展开更多
基金Project(41941018)supported by the National Natural Science Foundation of China for the Special Project FundingProject(22-JKCF-08)supported by the Study on in-situ Stress Database and 3D in-situ Stress Inversion Technology of Highway Tunnel in Shanxi Province,China+1 种基金Project(2022-JKKJ-6)supported by the Study on Disaster Mechanism and NPR Anchor Cable Prevention and Control of Coal Mining Caving Subsidence in Operating Tunnel in Mountainous Area,ChinaProject(BBJ2024032)supported by the Fundamental Research Funds for the Central Universities(PhD Top Innovative Talents Fund of CUMTB),China。
文摘A study was conducted to analyze the deformation mechanism of strongly weathered quartz schist in the Daliangshan Tunnel,located in the western Transverse Mountain area.A large deformation problem was experienced during the tunnel construction.To mitigate this problem,a support system was designed incorporating negative Poisson ratio(NPR)anchor cables with negative Poisson ratio effect.Physical model experiments,field experiments,and numerical simulation experiments were conducted to investigate the compensation mechanical behavior of NPR anchor cables.The large deformations of soft rocks in the Daliangshan Tunnel are caused by a high ground stress,a high degree of joint fracture development,and a high degree of surrounding rock fragmentation.A compensation mechanics support system combining long and short NPR anchor cables was suggested to provide sufficient counter-support force(approximately 350 kN)for the surrounding rock inside the tunnel.Comparing the NPR anchor cable support system with the original support system used in the Daliangshan tunnel showed that an NPR anchor cable support system,combining cables of 6.3 m and 10.3 m in length,effectively prevented convergence of surrounding rock deformation,and the integrated settlement convergence value remained below 300 mm.This study provides an effective scientific basis for resolving large deformation problems in deeply buried soft rocks in western transverse mountain areas.
文摘A new mechanics model, which reveals additional longitudinal force transmission between the continuously welded rails and the bridges, is established on the fact that the influence of the mutual relative displacement (among) the rail, the sleeper and the beam is taken into account. An example is presented and numerical results are compared. The results show that the additional longitudinal forces calculated with the new model are less than those of the previous, especially in the case of the flexible pier bridges. The new model is also suitable for the analysis of the additional longitudinal force transmission between rails and bridges of ballastless track with small resistance fasteners without taking the sleeper displacement into account, and compared with the ballast bridges, the ballastless bridges have a much stronger additional longitudinal force transmission between the continuously welded rails and the bridges.
基金Project(51109117)supported by the National Natural Science Foundation of ChinaProject(20111081125)supported by the Independent Research Plan of Tsinghua University,ChinaProject(2013-KY-4)supported by the State Key Laboratory of Hydroscience and Engineering Project,China
文摘The mesoscopic failure mechanism and the macro-mechanical characteristics of soil-rock mixture(S-RM) under external load are largely controlled by S-RM's meso-structural features. The objective of this work is to improve the three-dimensional technology for the generation of the random meso-structural models of S-RM, for randomly generating irregular rock blocks in S-RM with different shapes, sizes, and distributions according to the characteristics of the rock blocks' size distribution. Based on the new improved technology, a software system named as R-SRM3 D for generation and visualization of S-RM is developed. Using R-SRM3 D, a three-dimensional meso-structural model of S-RM is generated and used to study the meso-mechanical behavior through a series of true-triaxial numerical tests. From the numerical tests, the following conclusions are obtained. The meso-stress field of S-RM is influenced by the distribution of the internal rock blocks, and the macro-mechanical characteristics of S-RM are anisotropic in 3D; the intermediate principal stress and the soil-rock interface properties have significant influence on the macro strength of S-RM.
基金Projects(51323014,51479095)supported by the National Natural Science Foundation of ChinaProject(20111081125)supported by Independent Research Plan of Tsinghua University,ChinaProject(2013-KY-4)supported by the State Key Laboratory of Hydroscience and Engineering Project,China
文摘Soil-rock mixture(S-RM)is a widely distributed geotechnical medium composed of "soil" and "rock block" different both in size and strength. Internal rock blocks form special and variable meso-structural characteristics of S-RM. The objective of this work was to study the control mechanism of meso-structural characteristics on mechanical properties of S-RM. For S-RM containing randomly generated polygonal rock blocks, a series of biaxial tests based on DEM were conducted. On the basis of research on the effects of rock blocks' breakability and sample lateral boundary type(rigid, flexible) on macroscopic mechanical behavior of S-RM, an expanded Mohr-Coulomb criterion in power function form was proposed to represent the strength envelop. At the mesoscopic level, the variations of meso-structure such as rotation of rock block, and the formation mechanism and evolution process of the shear band during tests were investigated. The results show that for S-RM with a high content of rock block, translation, rotating and breakage of rock blocks have crucial effects on mechanical behavior of S-RM. The formation and location of the shear band inside S-RM sample are also controlled by breakability and arrangement of rock blocks.
文摘This paper deals with the mechanics problem of dynamic walking of anthropomorphic biped robots. Through analysing the mechanics system of this kind of robots in detail, the motion constraint equations are established, three mechanics laws describing the r
文摘Land subsidence hazard,which is caused by drawing groundwater,has been a problem of the world.In order to control the development of land subsidence,exploited horizon of groundwater was adjusted from the shallow layer to the deep layer in the past years.But the deformation mechanics study of the aquitard in different depth is not enough.There were essential distinctions between the shallow aquitard and the deep aquitard in engineering geology properties.The paper used the different depth aquitard of Tianjin city as an example to study the
文摘Cancer stem cells(CSCs)are the driving force for sustainable tumor growth and metastasis and responsible for drug resistance and cancer relapse.Nanoparticle-based drug delivery has been demonstrated to be effective in combating tumor growth.However,it has been challenging to selectively eliminate CSCs due to the lack of a general signature for a spectrum of cancers.It is known that CSCs from various types of cancer show lower stiffness compared to non-CSCs.It remains unclear whether low stiffness in CSCs influences cellular uptake in nanoparticle-based drug delivery and thus the chemotherapy efficacy.Graphene quantum dot(GQD)is emerging as a promising carrier material in delivering anti-cancer drugs.We found that breast CSCs were softer than conventional cancer cells,which were further softer compared to healthy breast tissue cells.Importantly,soft CSCs uptook more GQD than conventional cancer cells,while stiff breast cancer cells with relatively low stiffness uptook more GQD than healthy breast cells.Softening cells by pharmacologically inhibiting actomyosin activity using either siRNA or actomyosin inhibitors significantly enhanced the cellular uptake of GQD in breast cancer cells but not CSCs,while stiffening cells by activating actomyosin using CA-MLCK/ROCK or actomyosin activators considerably suppressed the nanoparticle uptake in both cancer cells and CSCs.GQD could specifically target CSC because of low cell stiffness of CSC in breast cancer cell line MCF-7 and MDA-MB-231.Further regulating cell stiffness reflected that decreasing breast cancer cell stiffness by inhibiting actomyosin activity using blebbistatin could promote GQD uptake.Vice versa,stiffening cancer cell by activating actomyosin decreased GQD uptake.The attachment of anti-cancer drug doxorubicin did not alter the trend of GQD uptake in neither soften nor stiffen cancer cells.Actomyosin activity regulates cellular uptake ofGQD might through clathrin and caveolin-mediated endocytosis.Cancer cells are softer than normal cells from the same organ,CSC are softer than non-CSC.Thus we further confirmed that the GQD uptake of normal breast cell line MCF-10 is less than breast cancer cell line MCF-7 and MDA-MB-231.Suggesting the clinical significance that using GQD as drug carrier targeting softer cells could reduce side effects to normal tissue cells.Since CSC are softer than non-CSC,GQD could decreased the percentage of CSC in whole cancer cell population by targeting softer cells.These results suggesting that it would be possible to target cancer cells and CSC by targeting from a perspective of cell mechanical difference.High uptake of nanoparticles in soft cancer cells could not be explained by their differential membrane potentials.Mechanistically,low cell mechanics or inhibiting actomyosin activity activated both clathrin and caveolin-mediated endocytosis signaling pathways,while high cell mechanics or activating actomyosin suppressed these signalings.Pharmacologically inhibiting clathrin or caveolin-mediated endocytosis signaling significantly decreased GQD uptake in CSCs and in conventional breast cancer cells when actomyosin was suppressed.Further,GQD conjugated with doxorubicin could be specifically delivered into CSCs with low stiffness and eliminated more CSCs in the presence of both CSCs and non-CSCs.Taken together,these data reveal the regulatory role of cell mechanics in cellular uptake of nanoparticles and demonstrate that GQD can be utilized to specifically eliminate CSCs,which have important implications in nanoparticle-based drug delivery for cancer therapy.
基金the support from National Natural Science Foundation of China ( 11672255)Shenzhen Science and Technology Innovation Commission ( JCYJ20170303160515987, JCYJ20170413154735522 )+1 种基金Early Career Scheme from Research Grants Council of the Hong Kong Special Administrative Region,China ( PolyU 252094 /17E)the internal grant from the Hong Kong Polytechnic University ( 1-ZE4Q,1-ZVJ8)
文摘Metastasis is the main cause of cancer death,and tumor cells mainly disseminate to the distal organs through the blood circulation,in which they experience considerable levels of fluid shear stress.CTCs are heterogeneous with diverse subpopulations of distinct genotypes and phenotypes and the frequency of CTCs is correlated with poor prognosis and overall survival in cancer patients.Less than 0.01%of them may eventually generate metastatic tumors in secondary sites,indicating the inefficiency of metastasis.Nevertheless,metastasis accounts for over 90%of cancer-related deaths,suggesting that a subpopulation of CTCs are able to survive the metastatic process and form metastases.To target metastasis,it is thus essential to understand the roles of various factors during dissemination in the survival and functions of CTCs.However,the effects of hemodynamic shear stress on biophysical properties and functions of CTCs in suspension are not fully understood.This study was to investigate the effect of hemodynamic shear stress on the survival and anti-chemotherapy ability of suspended circulating tumor cells during metastasis,and the effect of actomyosin activity on this regulation.In this study,we developed a circulatory system to generate physiologic levels of hemodynamic shear stress,which mimicked certain important aspects of the CTC microenvironment in blood circulation.The survival of tumor cells in suspension,as a model for real CTCs,under different shear stress and circulation duration was examined.We found that the majority of breast tumor cells s in suspension can be eliminated by hemodynamic shear stress.The surviving cells exhibit unique biophysical properties,including significantly retarded cell adhesion,mesenchymal-like cell morphology,and reduced F-actin expression and cellular stiffness.Cancer stem cells which has been reported in other papers have lower stiffness compared with conventional tumor cells showed significantly higher survival in blood flow.Importantly,low actomyosin activity promotes the survival of CTCs in blood shear flow while high actomyosin activity inhibits tumor cells surviving shear stress treatment.These findings might be explained by the up-and down-regulation of the anti-apoptosis genes.Soft surviving tumor cells held survival advantages in shear flow and higher resistance to chemotherapy.Metastasis is closely linked with chemoresistance.However,the underlying mechanisms have not been fully understood,in particular,the roles of hemodynamic shear stress and actomyosin-dependent cell mechanics in drug resistance of CTCs remain unclear.Inhibiting actomyosin activity in suspended tumor cells enhanced chemoresistance,while activating actomyosin suppressed this ability.These findings might be associated with the corresponding changes in multidrug resistance related genes.Our study unveils the regulatory roles of actomyosin in the survival and drug resistance of circulating tumor cells in hemodynamic shear flow,which imply the importance of fluid shear stress and actomyosin activity in tumor metastasis.Our findings reveal a new mechanism by which circulating tumor cells are able to survive hemodynamic shear stress and chemotherapy and may offer a new potential strategy to target circulating tumor cells in shear flow and combat chemoresistance through actomyosin.
文摘Recently, this study group established “the map of MOHO\|surface bathymetric line in Chinese and Near Region" on the basis of latest survey and study of the crustal depth, the preliminary result shows that the regular meridional and latitudinal upwarping and downwarping structural pattern of MOHO\|surface bathymetric line among Eurasian plate and Pacific plate and the Indian plate alternately appears, and which is accreted and coupled with basin ridge structure that exist shallow crustal base, continental crust and oceanic crust and others regular upwarping and downwarping net structure system that possessing different block characters and different scales exist together. Among different structure systems, it occurs that ramp downwarping impetus transform structure belts whose trends is characteristic. Nowadays upwarping and downwarping net structure system is basically modeled in Himalayan orogeny period. It is showed that the Earth revolution way has been changed in this period, which leaded to a new Earth dynamics cycle.The pattern of upwarping and downwarping structure among different structure systems or different structure blocks , and the characters of different trends and different scale transform structure belts, reflects the structure movement way and their conversion law, and reveals the Earth centralized dynamics mechanics that is produced by the revolutionary effect under the environments of aster system. This can be clearly reflected by the change of impetus way between Qinghai—Tibet highland structure system and near structure system.
文摘The rapid development of nanotechnology enables the successful application of target drug delivery,which provides new hope for the clinical examination and treatment.In the whole process of drug delivery,we foundcouple of mechanics problems existed,including the transportation of nanocarriers,the molecular level target and the cellular uptake of nanoparticles.In recent years,we focused on the investigation of diffusion of drug delivery systems in mucus of gastrointestinal(GI)tract and tumor intestinal.We studied the stiffness effect and geometric effect of nanoparticles in cell internalization,and found that the shape,stiffness and adhesion of nanoparticle-based drug carriers affect their transportation in biological tissues.We revealed the internal mechanism with a theoretical model for the diffusion of nanoparticles in an adhesive/nonadhesive polymer network.These findings shed new light on the design of NP-based drug delivery systems targeted to mucosal and tumor sites that possess a fibrous structure/porous medium.
文摘This article demonstrates a novel approach for material nonlinear analysis.This analysis procedure eliminates tedious and lengthy step by step incremental and then iterative procedure adopted classically and gives direct results in the linear as well as in nonlinear range of the material behavior.Use of elastic moduli is eliminated.Instead,stress and strain functions are used as the material input in the analysis procedure.These stress and strain functions are directly derived from the stress-strain behavior of the material by the method of curve fitting.This way,the whole stress-strain diagram is utilized in the analysis which naturally exposes the response of structure when loading is in nonlinear range of the material behavior.It is found that it is an excellent computational procedure adopted so far for material nonlinear analysis which gives very accurate results,easy to adopt and simple in calculations.The method eliminates all types of linearity assumptions in basic derivations of equations and hence,eliminates all types of possibility of errors in the analysis procedure as well.As it is required to know stress distribution in the structural body by proper modelling and structural idealization,the proposed analysis approach can be regarded as stress-based analysis procedure.Basic problems such as uniaxial problem,beam bending,and torsion problems are solved.It is found that approach is very suitable for solving the problems of fracture mechanics.Energy release rate for plate with center crack and double cantilever beam specimen is also evaluated.The approach solves the fracture problem with relative ease in strength of material style calculations.For all problems,results are compared with the classical displacement-based liner theory.
文摘The relationship between engine mechanics and thermo-dynamics has been investigated by means of numerical simulation.The inherent mismatching between the mechanical behaviors and the thermodynamic process in internal combustion engine is identified,which is believed to be one of the important limiting factors of energy efficiency for conventional engines available in the current market.An approach for engine efficiency improvement through optimal matching between mechanics and thermodynamics(OMBMT)is proposed.An ideal matching model is defined and the conflicts due to the constraints among the mapping strokes in a 4-stroke engine are analyzed.A novel mechanical model is built for approaching optimal matching among all 4 individual strokes in a 4-stroke spark-ignition engine,which is composed of non-circular gears(NCG)and integrated with conventional slider crank engine mechanism.By means of digital mechanical model and numerical simulation,the matching gains among all 4 strokes are defined and calculated for quantifying the NCG engine efficiency improvement by comparing with a baseline engine.The potentials with the OMBMT implemented and the enhancements made by NCG mechanism for engines in terms of overall engine efficiency are reported.Based on the results achieved,it is recommended that the feasibility studies and the experimental validations should be conducted to verify the engine matching concept and effectiveness of the NCG mechanism engine model proposed,and the engine performance and NCG design parameters should be further optimized.
文摘The finite element analysis was carried out for a composite vertical axis wind turbine with lift-drag combined starting structures to ensure the structure safety of a vertical axis wind turbine(VAWT).The static and modal analysis of rotor of a composite vertical axis wind turbine was conducted by using ANSYS software.The relevant contour sketch of stress and deformation was obtained.The analysis was made for static structural mechanics,modal analysis of rotor and the total deformation and vibration profile to evaluate the influence on the working capability of the rotor.The analysis results show that the various structure parameters lie in the safety range of structural mechanics in the relative standards.The analysis showing the design safe to operate the rotor of a vertical axis wind turbine.The methods used in this study can be used as a good reference for the structural mechanics′analysis of VAWTs.
文摘Focal adhesions(FAs) are large,multiprotein complexs that provides linkers between cytoskeleton to the extracellular matrix(ECM).Cells sense and respond to forces through FAs to regulate a broad range of processes,such as cell growth,migration,differentiation
基金supported by grants from the National Natural Science Foundation of China,Nos 10628205,10732050,and 10872115
文摘Cells sense and respond to forces and extracellular environment through FAs to regulate a broad range of processes, such as cell growth,migration,differentiation and apoptosis. Currently,the underlying mechanisms of the force
文摘The present study deals with the experimental,finite element(FE)and analytical assessment of low ballistic impact response of proposed flexible‘green’composite make use of naturally available jute and rubber as the constituents of the composite with stacking sequences namely jute/rubber/jute(JRJ),jute/rubber/rubber/jute(JRRJ)and jute/rubber/jute/rubber/jute(JRJRJ).Ballistic impact tests were carried out by firing a conical projectile using a gas gun apparatus at lower range of ballistic impact regime.The ballistic impact response of the proposed flexible composites are assesses based on energy absorption and damage mechanism.Results revealed that inclusion of natural rubber aids in better energy absorption and mitigating the failure of the proposed composite.Among the three different stacking sequences of flexible composites considered,JRJRJ provides better ballistic performance compared to its counterparts.The damage study reveals that the main mechanism of failure involved in flexible composites is matrix tearing as opposed to matrix cracking in stiff composites indicating that the proposed flexible composites are free from catastrophic failure.Results obtained from experimental,FE and analytical approach pertaining to energy absorption and damage mechanism agree well with each other.The proposed flexible composites due to their exhibited energy absorption capabilities and damage mechanism are best suited as claddings for structural application subjected to impact with an aim of protecting the main structural component from being failed catastrophically.
基金Project(52074299)supported by the National Natural Science Foundation of ChinaProjects(2023JCCXSB02,BBJ2024083)supported by the Fundamental Research Funds for the Central Universities,China。
文摘This study proposed a new and more flexible S-shaped rock damage evolution model from a phenomenological perspective based on an improved Logistic function to describe the characteristics of the rock strain softening and damage process.Simultaneously,it established a constitutive model capable of describing the entire process of rock pre-peak compaction and post-peak strain softening deformation,considering the nonlinear effects of the initial compaction stage of rocks,combined with damage mechanics theory and effective medium theory.In addition,this research verified the rationality of the constructed damage constitutive model using results from uniaxial and conventional triaxial compression tests on Miluo granite,yellow sandstone,mudstone,and glutenite.The results indicate that based on the improved Logistic function,the theoretical damage model accurately describes the entire evolution of damage characteristics during rock compression deformation,from maintenance through gradual onset,accelerated development to deceleration and termination,in a simple and unified expression.At the same time,the constructed constitutive model can accurately simulate the stress-strain process of different rock types under uniaxial and conventional triaxial compression,and the theoretical model curve closely aligns with experimental data.Compared to existing constitutive models,the proposed model has significant advantages.The damage model parameters a,r and β have clear physical meanings and interact competitively,where the three parameters collectively determine the shape of the theoretical stress−strain curve.
