This study investigated the growth situation of Listeria monocytogenes on chilled pork and the effect of herbal preservatives on this pathogen. The inhibitions of herbal preservatives were identified. The minimum inhi...This study investigated the growth situation of Listeria monocytogenes on chilled pork and the effect of herbal preservatives on this pathogen. The inhibitions of herbal preservatives were identified. The minimum inhibitory concentrations （MIC） of cinnamon and clove were all 0.79 mg.mL^-1, while the rosemary was 1.58 mg.mL^-1. And the composite herbal preservatives were got through orthogonal experiment. The optimum proportion was as following on agar medium： 1.16 mg.mL^-1 cinnamon＋2.38 mg. mL^-1 rosemary＋3.17 mg. mLl clove （herb combination number 5）, while on chilled pork, the strong inhibition of L. monocytogenes was showed, which demonstrated that the surface application of herb combination resulted in an effective delay of L. monocytogenes growth.展开更多
该研究旨在探究不同NaCl浓度对单增李斯特菌和副溶血性弧菌混合生物被膜形成的影响。测定了不同NaCl浓度下单种和混合生物被膜的生物量、代谢活性,以及生物被膜和浮游菌的细胞组成,分析了两菌的相互作用。结果表明,与单种生物被膜相比,...该研究旨在探究不同NaCl浓度对单增李斯特菌和副溶血性弧菌混合生物被膜形成的影响。测定了不同NaCl浓度下单种和混合生物被膜的生物量、代谢活性,以及生物被膜和浮游菌的细胞组成,分析了两菌的相互作用。结果表明,与单种生物被膜相比,混合生物被膜的形成能力和代谢活性较强;在0.5 g/100 mL NaCl浓度的胰酪大豆胨液体培养基中,优势菌株为单增李斯特菌;而在1~5 g/100 mL NaCl浓度的混合培养体系中,副溶血性弧菌是优势菌株,单增李斯特菌对副溶血性弧菌生物被膜形成具有促进作用,而副溶血性弧菌对单增李斯特菌具有抑制作用,且在1.5 g/100 mL NaCl浓度下两者相互作用最强。综上,副溶血性弧菌是优势菌株,在混合生物被膜中占主导地位。研究结果为控制单增李斯特菌和副溶血性弧菌混合生物被膜污染提供理论依据。展开更多
基金the Student Abroad Foundation of Harbin (RC2006LX020003)
文摘This study investigated the growth situation of Listeria monocytogenes on chilled pork and the effect of herbal preservatives on this pathogen. The inhibitions of herbal preservatives were identified. The minimum inhibitory concentrations （MIC） of cinnamon and clove were all 0.79 mg.mL^-1, while the rosemary was 1.58 mg.mL^-1. And the composite herbal preservatives were got through orthogonal experiment. The optimum proportion was as following on agar medium： 1.16 mg.mL^-1 cinnamon＋2.38 mg. mL^-1 rosemary＋3.17 mg. mLl clove （herb combination number 5）, while on chilled pork, the strong inhibition of L. monocytogenes was showed, which demonstrated that the surface application of herb combination resulted in an effective delay of L. monocytogenes growth.
文摘该研究旨在探究不同NaCl浓度对单增李斯特菌和副溶血性弧菌混合生物被膜形成的影响。测定了不同NaCl浓度下单种和混合生物被膜的生物量、代谢活性,以及生物被膜和浮游菌的细胞组成,分析了两菌的相互作用。结果表明,与单种生物被膜相比,混合生物被膜的形成能力和代谢活性较强;在0.5 g/100 mL NaCl浓度的胰酪大豆胨液体培养基中,优势菌株为单增李斯特菌;而在1~5 g/100 mL NaCl浓度的混合培养体系中,副溶血性弧菌是优势菌株,单增李斯特菌对副溶血性弧菌生物被膜形成具有促进作用,而副溶血性弧菌对单增李斯特菌具有抑制作用,且在1.5 g/100 mL NaCl浓度下两者相互作用最强。综上,副溶血性弧菌是优势菌株,在混合生物被膜中占主导地位。研究结果为控制单增李斯特菌和副溶血性弧菌混合生物被膜污染提供理论依据。
