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Design of a wideband non-uniform microstrip line for complex impedance matching in automotive radar frequencies
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作者 ZHANG Shuang-Gen YU Tao +2 位作者 WANG Yu-Lan CHENG Zhi-Hua YAO Jian-Quan 《红外与毫米波学报》 北大核心 2025年第2期168-176,共9页
Microstrip transmission lines connecting to the millimeter wave radar chip and antenna significantly affect the radiation efficiency and bandwidth of the antenna.Here,a wideband non-uniform wavy microstrip line for co... Microstrip transmission lines connecting to the millimeter wave radar chip and antenna significantly affect the radiation efficiency and bandwidth of the antenna.Here,a wideband non-uniform wavy microstrip line for complex impedance in automotive radar frequency range is proposed.Unlike the gradient transmission line,the wavy structure is composed of periodically semi-circular segments.By adjusting the radius of the semi-circular,the surface current is varied and concentrated on the semi-circular segments,allowing a wider tunability range of the resonant frequency.The results reveal that the bandwidth of the loaded wavy transmission line antenna improves to 9.37 GHz,which is 5.81 GHz wider than that of the loaded gradient line.The gain and the half power beam width of the loaded antenna are about 14.69 dB and 9.58°,respectively.The proposed non-uniform microstrip line scheme may open up a route for realizing wideband millimeter-wave automotive radar applications. 展开更多
关键词 millimeter wave gradient line wavy transmission line broadband high gain array antenna
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Design of W-band microstrip dual-channel traveling wave tubes
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作者 Wang Zhanliang Zhou Shuaicen +4 位作者 Lu Zhigang Gong Huarong Gong Yubin Su Xiaogang Feng Jinjun 《强激光与粒子束》 北大核心 2025年第8期67-72,共6页
Microstrip traveling wave tubes(TWTs)have garnered significant attention due to their potential applications in communication,defense,and industrial systems.This paper presents a compact W-band dual-channel TWT,utiliz... Microstrip traveling wave tubes(TWTs)have garnered significant attention due to their potential applications in communication,defense,and industrial systems.This paper presents a compact W-band dual-channel TWT,utilizing a U-shaped microstrip meander-line slow-wave structure(SWS).High-frequency characteristics are analyzed through simulation and cold tests.The results demonstrate that adjusting structural parameters effectively optimizes the S-parameters.Particle-in-cell(PIC)simulations with an 18.8 kV,0.1 A electron beam predict an output power of 18 W with a gain of 14 dB.Experimental measurements of S-parameters are conducted using three substrate materials:Rogers 5880,quartz,and diamond.The quartz substrate exhibits the closest agreement with simulation results.The results advance the development of the microstrip-based TWTs for high-data-rate communication systems. 展开更多
关键词 TWT MILLIMETER-WAVE meander line multi-channel amplifier
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A new explicit construction of unique-neighbor expanders
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作者 Weiqi Zhu 《中国科学技术大学学报》 北大核心 2025年第5期51-60,50,I0002,共12页
For every integer 4≤d≤11,an explicit construction of infinite families of 2d-regular unique-neighbor expanders is presented,which is a generalization of the 6-regular unique-neighbors initially developed by Alon and... For every integer 4≤d≤11,an explicit construction of infinite families of 2d-regular unique-neighbor expanders is presented,which is a generalization of the 6-regular unique-neighbors initially developed by Alon and Capalbo.Additionally,for values of d greater than 11,a sufficient condition is established for employing the same construction method.Our construction method involves the“line product”of large bipartite Ramanujan graphs and a sufficiently good unique-neighbor expander(a small gadget). 展开更多
关键词 unique-neighbor expander Ramanujan graph line product
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基于网络表示学习算法的知识追踪模型
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作者 陈倩倩 《计算机应用与软件》 北大核心 2025年第5期30-35,87,共7页
针对学习过程一致的知识追踪模型中存在未考虑习题难度的问题,提出一种以回答习题时间和错误率的加权平均结果作为评价指标的量化难度的方法;针对学习过程一致的知识追踪模型中知识概念彼此独立脱离现实的问题,提出用网络结构表示知识概... 针对学习过程一致的知识追踪模型中存在未考虑习题难度的问题,提出一种以回答习题时间和错误率的加权平均结果作为评价指标的量化难度的方法;针对学习过程一致的知识追踪模型中知识概念彼此独立脱离现实的问题,提出用网络结构表示知识概念,基于联合概率和经验分布计算知识概念节点间存在的一阶相似性和二阶相似性,利用网络嵌入中的LINE算法可以保留网络结构信息的优势,将网络结构表示的知识概念嵌入到低维特征向量。通过在真实公开的数据上建立模型进行实验,验证了方法的有效性。 展开更多
关键词 知识概念 问题难度 一阶相似性 二阶相似性 LINE算法 网络表示学习
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Numerical study on the water inflow and hydraulic pressure of mountain tunnel underpassing a reservoir
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作者 LIU Dongdong SONG Wenjie +3 位作者 WANG Xintong YANG Tao HUANG Bo ZHONG GUO 《中国水利水电科学研究院学报(中英文)》 北大核心 2025年第5期580-589,共10页
Prediction of water inflow into a tunnel is a crucial prerequisite for the waterproof and drainage design of mountain tunnels in water-rich areas.Based on the proposed Baiyun Mountain Tunnel project in Guangzhou,a num... Prediction of water inflow into a tunnel is a crucial prerequisite for the waterproof and drainage design of mountain tunnels in water-rich areas.Based on the proposed Baiyun Mountain Tunnel project in Guangzhou,a numerical percolation model of random fractured rock of a tunnel underpassing a water reservoir is established to study the seepage characteristics of surrounding rock,the law of water inflow,and the change of lining water pressure,considering the local artificial boundary conditions for seepage in large rock mass,.In addition,the influences of rock permeability,fracture aperture,grouting circle thickness,and penetration are analyzed.The results show that:(1)Only fractures with aperture wider than 0.1 mm can play a significant role in water conduction in rocks with the permeability lower than 10^(-11)m^(2);(2)The greater the permeability difference between the fractures and rocks,the more remarkable the effects of fractures on the surrounding rock seepage field and cavern water inflow;(3)The sensitivity of grouting waterproof function to grouting circle thickness,grouting ring penetration,and rock permeability is significantly higher than that of tunnel buried depth and fracture aperture;(4)The lining water head is much more sensitive to the grouting circle thickness and penetration than to the tunnel buried depth;(5)With the grouting range enlarging,the impact of grouting circle permeability on the precipitation pressure role of the grouting ring increases;(6)For the interesting tunnel designed to be built at the depth of 70 m,the grouting circle with the thickness of 0.5 m and permeability of 10-^(14)m^(2)is recommended. 展开更多
关键词 mountain tunnel water inflow into a tunnel lining water pressure grouting circle stochastic fracture networks
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Evaluation of transgenic cotton lines expressing an insecticidal fern protein against whitefly,Bemisia tabaci(Gennadius)
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作者 KUMAR Rishi NAGRARE V.S. +14 位作者 SHAH Vivek SINGH Satnam PANDHER Suneet SINGH Satpal VERMA S.K. PAUL Debashis RATHORE Pankaj SHUKLA Anoop Kumar SINGH Mithlesh Kumar SAURABH Sharad KUMAR Harish KAUR Rupinderjeet SINGH Pradhyumna Kumar WAGHMARE V.N. PRASAD Y.G. 《Journal of Cotton Research》 2025年第2期163-177,共15页
Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with des... Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with desirable traits that wouldn’t be possible through conventional breeding methods.Transgenic crops have been developed for various traits globally.Whitefly,Bemisia tabaci(Gennadius)is one of the major sucking pests of cotton that cause significant damage to the cotton production.To combat whitefly infestations,researchers have developed four transgenic cotton lines expressing the fern protein.And those transgenic lines need to be evaluated for their performance against the target pest—whitefly.The evaluation was designed as controlled trials in polyhouse or muslin cloth cages under open-choice and no-choice conditions by comparing four transgenic cotton lines(A,B,C,and D)with three control groups,including untransformed cotton plants with a same genetic background of the transgenic line,conventionally bred whitefly-resistant cotton,and whitefly-susceptible cotton.In order to study the generational effect,the evaluation also involved studies on whitefly development in laboratory,muslin cloth cage,and polyhouse conditions.Results Both open-choice and no-choice experiments had shown that all the four transgenic cotton lines(A,B,C,and D)expressing the fern protein reduced adult whitefly numbers significantly compared with the control lines,except for the no-choice conditions in 2021,where the transgenic line C was non-significant different from the resistant control line.Notably,the nymphal population on the resistant control line was relatively low and nonsignificant different from the transgenic line C in 2021;and the transgenic lines A and C in 2022 under open-choice conditions.Under no-choice condition,the nymphal counts in the resistant control line was non-significant different from transgenic lines C and D in 2021;and transgenic line D in 2022.All transgenic lines showed significant decrease in egg hatching in 2021 and nymphal development in 2022,except for the transgenic line C which had no significant different in the nymphal development comparing with non-transgenic control lines in 2022.Adult emergence rates in both years of evaluation showed significant decrease in transgenic lines A and B comparing with the control lines.Additionally,the results showed a significant reduction in cotton leaf curl disease and sooty mold development in all the four transgenic lines compared with susceptible control under open-choice conditions,indicating potential benefits of transgenic lines beyond direct effect on whitefly control.Furthermore,the research explored the generational effects of the fern protein on whitefly which revealed the lowest fecundity in the transgenic line C across F0,F1 and F3 generations,lower egg hatching in F1 and F2 generations in transgenic lines A and B,shorter nymphal duration in F1 and F2 generations in transgenic line B,and the least total adult emergence in the transgenic line C in F0 and F3 generations.Conclusions These findings suggest that the transgenic cotton lines expressing fern protein disrupts whitefly populations and the life cycle to a certain extent.However,results are not consistent over generations and years of study,indicating these transgenic lines were not superior over control lines and need to be improved in future breeding. 展开更多
关键词 EVALUATION Fern protein Transgenic cotton lines WHITEFLY
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Undercutting mechanism of worm wheel in offsetting normal arc-toothed cylindrical worm drive
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作者 MENG Qing-xiang JIAO Yu-ge +3 位作者 ZHAO Ya-ping MU Shi-bo CUI Jian ZHANG Ming-hua 《Journal of Central South University》 2025年第2期495-508,共14页
The worm wheel whose undercutting characteristic is researched is a member of offsetting normal arc-toothed cylindrical worm drive.The tooth profile of the worm in its offsetting normal section is a circular arc.The n... The worm wheel whose undercutting characteristic is researched is a member of offsetting normal arc-toothed cylindrical worm drive.The tooth profile of the worm in its offsetting normal section is a circular arc.The normal vector used to calculate the first-type limit function is determined in the natural frame without the aid of the curvature parameter of worm helicoid.The first-type limit line is ascertained via solving the nonlinear equations iteratively.It is discovered that one first-type limit line exists on the tooth surface of worm wheel by numerical simulation,and such a line is normally located out of the meshing zone.Only one intersection point exists between the first and second-types of limit lines,and this point is a lubrication weak point.The undercutting mechanism is essentially that a part of the meshing zone near the conjugated line of worm tooth crest will come into the undercutting area and will be cut off during machining the worm wheel.The machining simulation verifies the correctness of undercutting mechanism.Moreover,a convenient and practical characteristic quantity is proposed to judge whether the undercutting exists in the whole meshing zone via computing the first-type limit function values on the worm tooth crest. 展开更多
关键词 cylindrical worm drive undercutting mechanism limit line nonlinear equations meshing zone
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Carbonated water erosion characteristics and mechanism of tunnel lining cement-based materials in karst environment
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作者 ZOU Min LIU Juan-hong LI Kang 《Journal of Central South University》 2025年第8期3015-3034,共20页
The study aims to investigate the carbonated water erosion mechanism of lining concrete in tunnels traversing karst environment and enhance its resistance.In this study,dynamic carbonated water erosion was simulated t... The study aims to investigate the carbonated water erosion mechanism of lining concrete in tunnels traversing karst environment and enhance its resistance.In this study,dynamic carbonated water erosion was simulated to assess erosion depth,microstructure,phase migrations,and pore structure in various tunnel lining cement-based materials.Additionally,Ca^(2+)leaching was analyzed,and impact of Ca/Si molar ratio in hydration products on erosion resistance was discussed by thermodynamic calculations.The results indicate that carbonated water erosion caused rough and porous surface on specimens,with reduced portlandite and CaCO_(3) content,increased porosity,and an enlargement of pore size.The thermodynamic calculations indicate that the erosion is spontaneous,driven by physical dissolution and chemical reactions dominated by Gibbs free energy.And the erosion reactions proceed more spontaneously and extensively when Ca/Si molar ratio in hydration products was higher.Therefore,cement-based materials with higher portlandite content exhibit weaker erosion resistance.Model-building concrete,with C-S-H gel and portlandite as primary hydration products,has greater erosion susceptibility than shotcrete with ettringite as main hydration product.Moreover,adding silicon-rich mineral admixtures can enhance the erosion resistance.This research offers theory and tech insights to boost cement-based material resistance against carbonated water erosion in karst tunnel engineering. 展开更多
关键词 tunnel lining cement-based materials carbonated water erosion phase analysis pore structure Ca/Si molar ratio
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直流GIL中自由线形金属微粒的运动与放电特性 被引量:36
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作者 王健 李庆民 +2 位作者 李伯涛 刘思华 王志远 《中国电机工程学报》 EI CSCD 北大核心 2016年第17期4793-4800,共8页
针对直流GIL中较常见且具有严重影响的自由线形金属微粒,搭建了贴合实际工况的封闭式同轴圆柱电极平台,同时使用高速相机和局放监测手段观测线形金属微粒的运动、局放与击穿行为。定量获得了微粒长度对站立与跳跃两种不同运动模式发生... 针对直流GIL中较常见且具有严重影响的自由线形金属微粒,搭建了贴合实际工况的封闭式同轴圆柱电极平台,同时使用高速相机和局放监测手段观测线形金属微粒的运动、局放与击穿行为。定量获得了微粒长度对站立与跳跃两种不同运动模式发生概率的影响;拍摄了站立线形微粒引起的电晕图像,同时监测、提取并统计微粒引起的局部放电情况;记录金属微粒引起的击穿图像,并基于流注理论分析了自由线形金属微粒引起的气隙击穿特性。研究表明:线形微粒的起动场强与微粒的长度基本无关,而与微粒的半径开方成正比,微粒越短,起动后越易达到跳跃运动状态,而微粒越长,越易保持站立状态;微粒引起的局部放电随着微粒长度的增大而更加剧烈;对于跳跃的自由线形微粒,其引起的微放电击穿间距随着微粒长度的增加而增加,击穿电压随着微粒长度的增加而降低。 展开更多
关键词 直流气体绝缘金属封闭输电线路(gas insulated metal enclosed transmission line GIL) 自由线形金属微粒 运动模式 局部放电特征 微放电间距
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非线性传输线产生射频脉冲原理研究 被引量:8
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作者 谢平 徐刚 +2 位作者 廖勇 石小燕 杨周炳 《强激光与粒子束》 EI CAS CSCD 北大核心 2014年第4期5-9,共5页
介绍了非线性传输线的工作原理和色散特性,给出了用于模拟交叉耦合磁饱和非线性传输线的计算方法。算法基于传输线各节点的时域差分方程组进行步进迭代,其中利用J-A模型描述传输线中NiZn铁氧体磁芯的非线性磁化行为,并模拟了非线性传输... 介绍了非线性传输线的工作原理和色散特性,给出了用于模拟交叉耦合磁饱和非线性传输线的计算方法。算法基于传输线各节点的时域差分方程组进行步进迭代,其中利用J-A模型描述传输线中NiZn铁氧体磁芯的非线性磁化行为,并模拟了非线性传输线的工作方式。实验获得了中心频率165MHz的宽带脉冲输出,初步验证了用于产生宽带电磁脉冲的非线性传输线关键技术。 展开更多
关键词 Key WORDS nonlinear transmission line simulation method J A model high vohage RADIO frequency PULSE MAGNET LC SATURATION
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CoLM模式在西北干旱区和青藏高原区的适用性研究 被引量:28
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作者 辛羽飞 卞林根 张雪红 《高原气象》 CSCD 北大核心 2006年第4期567-574,共8页
运用CoLM(CLM3.0)模式对典型干旱区(张掖站)和青藏高原区(安多站、MS3478站)进行了off-line验证试验,检验了CoLM模式在典型干旱区和青藏高原区的性能。结果表明:CoLM模式对典型干旱区绿洲灌溉农业的陆面过程模拟较好,土壤各层温度的日... 运用CoLM(CLM3.0)模式对典型干旱区(张掖站)和青藏高原区(安多站、MS3478站)进行了off-line验证试验,检验了CoLM模式在典型干旱区和青藏高原区的性能。结果表明:CoLM模式对典型干旱区绿洲灌溉农业的陆面过程模拟较好,土壤各层温度的日变化趋势和季节变化趋势都能较准确地模拟出来,模拟的地表温度与观测值相关系数为0.885,均方差为0.873;模拟的5 cm土壤温度值与实测值的相关系数为0.944,均方差为0.891。在高原地区能量平衡分量的模拟中,净辐射、感热模拟效果较好,潜热分量模拟值偏大,故将CoLM模式用于高原地区还有待进一步改进。 展开更多
关键词 CoLM模式 陆面过程 黑河实验 GAME-Tibet试验 Off—line试验
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基于“猫眼效应”的超视距视轴对准可行性分析 被引量:4
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作者 葛成良 张凯 +2 位作者 叶一东 范国滨 梁正 《强激光与粒子束》 EI CAS CSCD 北大核心 2005年第11期1635-1638,共4页
根据光电探测器的“猫眼效应”,针对成像光学系统的视轴,从理论上分析了利用激光对超视距系统进行视轴对准的可行性,特别分析了激光器输出功率参数、接收探测器焦平面是否离焦等关键参数。研究结果表明,基于“猫眼效应”的超视距系统进... 根据光电探测器的“猫眼效应”,针对成像光学系统的视轴,从理论上分析了利用激光对超视距系统进行视轴对准的可行性,特别分析了激光器输出功率参数、接收探测器焦平面是否离焦等关键参数。研究结果表明,基于“猫眼效应”的超视距系统进行视轴对准在理论上和实验设计上是可行的。 展开更多
关键词 “猫眼效应” 超视距 视轴(LOS LINE of sight) 对准
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新麦系列新品种(系)灰色关联度分析 被引量:14
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作者 王映红 董昀 +3 位作者 程兰兰 蒋志凯 马华平 付亮 《山东农业科学》 2014年第2期32-35,共4页
对8个小麦新品种(系)的产量和其7个主要性状进行灰色关联度分析,以明确新麦系列新品种(系)产量和产量构成因素及其它性状之间的关系,为优势新品系的评价提供依据。结果表明:影响产量的7个因素,2012年的排序为穗粒数>千粒重>单位... 对8个小麦新品种(系)的产量和其7个主要性状进行灰色关联度分析,以明确新麦系列新品种(系)产量和产量构成因素及其它性状之间的关系,为优势新品系的评价提供依据。结果表明:影响产量的7个因素,2012年的排序为穗粒数>千粒重>单位面积穗数、成穗率>株高>春季最大分蘖数>基本苗数,2013年的排序为成穗率>单位面积穗数>千粒重>株高>穗粒数>基本苗数>春季最大分蘖数;两年关联度均靠前的小麦新品种(系)有新科麦169、新麦212。 展开更多
关键词 小麦品种(系) 产量 产量构成因素 灰色关联度分析 WHEAT VARIETIES ( lines)
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优良玉米自交系QR273改良系的遗传特征及其改良效果 被引量:6
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作者 胡兴 陈泽辉 +4 位作者 郭向阳 祝云芳 王安贵 赵丽 兰琴英 《贵州农业科学》 CAS 北大核心 2014年第2期35-38,共4页
为了进一步利用和改良热带玉米自交系QR273,选用4个具有Suwan血缘的优良自交系QB572、QB576、QB48和T32作为供体,QR273作为受体进行改良,对改良系农艺性状进行统计分析。结果表明:选育的30个改良系之间农艺性状差异均达显著水平,遗传变... 为了进一步利用和改良热带玉米自交系QR273,选用4个具有Suwan血缘的优良自交系QB572、QB576、QB48和T32作为供体,QR273作为受体进行改良,对改良系农艺性状进行统计分析。结果表明:选育的30个改良系之间农艺性状差异均达显著水平,遗传变异丰富;以QB48和QB576作为供体改良系的穗长、穗粗、株高、穗位高和吐丝期比QR273得到明显的改良;经改良系与QB506组配的杂交组合鉴定,增产最多是QB2201,比对照增加9.91%;其次是QB1704(6.23%)、QB2157(3.20%)、QB2159(3.13%)、QB2178(1.84%)和QB1775(0.63%)。 展开更多
关键词 玉米 自交系 QR273改良系 遗传特征 改良效果 IMPROVED LINES of QR273
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基于Color Lines先验的高阶马尔科夫随机场去雾 被引量:6
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作者 毕笃彦 眭萍 +1 位作者 何林远 马时平 《电子与信息学报》 EI CSCD 北大核心 2016年第9期2405-2409,共5页
传统的一阶马尔科夫随机场在图像先验信息表达和对图像整体的约束上能力有限,同时基于暗通道的去雾算法在天空等大片白色区域处理效果存在偏差。针对以上问题,该文提出一种基于Color Lines的高阶马尔科夫随机场去雾算法。该算法通过引... 传统的一阶马尔科夫随机场在图像先验信息表达和对图像整体的约束上能力有限,同时基于暗通道的去雾算法在天空等大片白色区域处理效果存在偏差。针对以上问题,该文提出一种基于Color Lines的高阶马尔科夫随机场去雾算法。该算法通过引入对颜色失真具有很好鲁棒性的Color Lines先验条件,初步校正经暗通道获取的传输图,然后利用高阶马尔科夫随机场优化传输图,获取最终精确的去雾图像。实验结果表明,与已有算法相比,该文算法具有更强的普适性,可提高雾天图像的清晰度,同时恢复更多的图像细节等信息。 展开更多
关键词 图像去雾 暗通道先验 高阶马尔科夫随机场 COLOR LINES
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多AUV队形控制的新方法 被引量:7
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作者 吴小平 冯正平 朱继懋 《舰船科学技术》 北大核心 2008年第2期128-134,共7页
基于机器人队形控制中的领航者-跟随者方法(Leader-Follower Method)和基于行为方法(Behavior-based Method),提出了一种用于多自治水下机器人(AUV)队形控制的新方法。该方法以领航者来确定整个AUV编队的前进状态,跟随者时刻跟随领航者... 基于机器人队形控制中的领航者-跟随者方法(Leader-Follower Method)和基于行为方法(Behavior-based Method),提出了一种用于多自治水下机器人(AUV)队形控制的新方法。该方法以领航者来确定整个AUV编队的前进状态,跟随者时刻跟随领航者以保持一定的队形形状。同时,领航者和跟随者都被赋予一定的行为特性以实现奔向目标、队形维持、队形转换及障碍避碰等功能。考虑到领航者在行进过程中有可能会发生意外而导致任务失败,本方法设定一特殊的跟随者令其作为候选领航者时刻跟随领航者以防万一。分别采用模糊规划器和Line of Sight(LOS)技术来实现AUV编队的障碍避碰和导航。 展开更多
关键词 多AUV 队形控制 LINE of SIGHT
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基于线扩散函数矩阵的成像光谱仪图像矫正方法 被引量:4
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作者 崔毅 危峻 黄小仙 《红外技术》 CSCD 北大核心 2014年第2期115-119,共5页
大视场推扫式成像光谱仪的驻留时间长,覆盖范围大,能比较好地满足海洋遥感的要求。但沿轨和穿轨方向上调制传递函数的差异导致了成像中的"拖影"现像。通过实验获取了系统的线扩散函数;利用插值法构建矩阵来描述系统穿轨方向... 大视场推扫式成像光谱仪的驻留时间长,覆盖范围大,能比较好地满足海洋遥感的要求。但沿轨和穿轨方向上调制传递函数的差异导致了成像中的"拖影"现像。通过实验获取了系统的线扩散函数;利用插值法构建矩阵来描述系统穿轨方向上的成像特性;使用遥感图像"拖影"区域的梯度平方和作为判别依据对矩阵的系数进行调整;最后使用矩阵对遥感图像进行了矫正。从矫正后的图像来看,"拖影"现像已基本被扣除,证实了该方法的有效性。 展开更多
关键词 线扩散函数 成像光谱仪 图像矫正 LINE SPREAD Function(LSF)
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黑皮冬瓜LINE逆转座子RT序列的克隆与特征分析 被引量:2
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作者 赵芹 谢大森 +3 位作者 江彪 何晓明 罗少波 李明珠 《热带作物学报》 CSCD 北大核心 2015年第3期466-473,共8页
根据LINE逆转座子逆转录酶保守序列设计简并引物,PCR扩增黑皮冬瓜“B98K”基因组DNA,获得580 bp左右目的条带。将PCR产物回收、克隆并测序,获得23条逆转酶序列,利用生物信息学软件分析其长度变异、碱基变化、相似性及系统进化关系。结... 根据LINE逆转座子逆转录酶保守序列设计简并引物,PCR扩增黑皮冬瓜“B98K”基因组DNA,获得580 bp左右目的条带。将PCR产物回收、克隆并测序,获得23条逆转酶序列,利用生物信息学软件分析其长度变异、碱基变化、相似性及系统进化关系。结果表明:这些序列长度在557~593 bp区间变异,同源比对核苷酸序列相似性为39.0%~99.3%,存在高度异质性,主要表现为缺失突变、移码突变与终止密码子突变,核苷酸序列聚类分析分为4个家族,家族1与家族2分别包含15和5个成员,占总序列数的65.22%和21.74%。对推导的氨基酸序列分析发现,第12位氨基酸残基处存在一保守的苯丙氨酸(Phe),多处位置存在半保守氨基酸残基;氨基酸序列相似性在20.0%~99.5%之间;其中8条序列可能具有转录活性,8条与15条序列分别发生移码与终止密码子突变。与已知物种构建系统发育进化树,发现冬瓜LINE逆转座子RT序列较保守,且与拟南芥、李、油菜等有较近的亲缘关系。研究结果为后续利用分子标记研究冬瓜种质遗传变异及基因组进化奠定基础。 展开更多
关键词 黑皮冬瓜 LINE逆转座子 逆转录酶 序列分析
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植物基因组中的非LTR反转录转座子SINEs和LINEs 被引量:7
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作者 程旭东 凌宏清 《遗传》 CAS CSCD 北大核心 2006年第6期731-736,共6页
反转录转座子是基因组进化的推动者之一,分为LTR和非LTR两种类型。前者是真核基因组的主要组分,结构和转座方式与逆转录病毒类似。后者是最初发现于动物基因组新近发现在植物基因组中也广泛存在的新型重复序列,包括LINEs(long intersper... 反转录转座子是基因组进化的推动者之一,分为LTR和非LTR两种类型。前者是真核基因组的主要组分,结构和转座方式与逆转录病毒类似。后者是最初发现于动物基因组新近发现在植物基因组中也广泛存在的新型重复序列,包括LINEs(long interspersed nuclear elements)和SINEs(short interspersed nuclear elements)两个亚型。它们大多因自身或受宿主基因组的调控而失去转座活性。其转座机理目前还不十分清楚,推测LINEs可以自主转座,SINEs依赖其他转座子被动转座。种系分析认为LINEs可能是最古老的反转录转座子,SINEs的起源未知。文章对以上内容进行了归纳和讨论。 展开更多
关键词 非LTR反转录转座子 LINES SINES 逆转录酶
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硒对鱼类生物学作用研究 被引量:3
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作者 曹艳林 柯浩 +1 位作者 刘振兴 林敏 《饲料工业》 北大核心 2010年第14期52-56,共5页
硒是生态环境中重要的微量元素和必需的生命元素,环境中硒的过量或缺乏均会导致人体和动物产生疾病。20世纪30年代,美欧一些地区由于过量硒引起的牲畜“碱毒病”(Alka—line Disease)和“蹒跚症”(Blind Stagger)使硒的毒害问题... 硒是生态环境中重要的微量元素和必需的生命元素,环境中硒的过量或缺乏均会导致人体和动物产生疾病。20世纪30年代,美欧一些地区由于过量硒引起的牲畜“碱毒病”(Alka—line Disease)和“蹒跚症”(Blind Stagger)使硒的毒害问题引起关注,迄止50年代,硒一直被认为是重要的环境污染元素。 展开更多
关键词 生物学作用 鱼类 生态环境 生命元素 微量元素 LINE 污染元素
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