The anti-hair loss mechanism of Aquilaria sinensis leaf extract(ASE)has been studied by using metabolomics and network pharmacology.Metabolomics was utilized to comprehensively identify the active constituents of ASE,...The anti-hair loss mechanism of Aquilaria sinensis leaf extract(ASE)has been studied by using metabolomics and network pharmacology.Metabolomics was utilized to comprehensively identify the active constituents of ASE,and the network pharmacology was used to elucidate their anti-hair loss mechanism,which was verified by molecular docking technology.572 active compounds were identified from the ASE by metabolomics methods,where there are 1447 corresponding targets and 492 targets related to hair loss,totaling 88 targets.20 core active substances were identified by constructing a network between common targets and active substances,which include vanillic acid,chorionic acid,caffeic acid and apigenin.The five key targets of TNF,TP53,IL6,PPARG,and EGFR were screened out by the PPI network analysis on 88 common targets.The GO and KEGG pathway enrichment analysis showed that the inflammation,hormone balance,cell growth,proliferation,apoptosis,and oxidative stress are involved.Molecular docking studies have confirmed the high binding affinity between core active compounds and key targets.The drug similarity assessment on these core compounds suggested that they have the potential to be used as potential hair loss treatment drugs.This study elucidates the complex molecular mechanism of ASE in treating hair loss,and provides a reference for the future applications in hair care products.展开更多
Soybean frogeye leaf spot(FLS)disease is a worldwide disease caused by Cercospora sojina Hara.It is one of the major diseases suffered by soybean during the growth cycle,which seriously damages the yield and seed qual...Soybean frogeye leaf spot(FLS)disease is a worldwide disease caused by Cercospora sojina Hara.It is one of the major diseases suffered by soybean during the growth cycle,which seriously damages the yield and seed quality of soybean.The current resistant varieties are difficult to meet the production demand.The breeders have identified 50 different physiological small species and discussed the physiological and biochemical characteristics of soybean resistance to FLS.In soybean disease resistance breeding,resistance resources are screened for the main physiological races in different countries,resistance materials are created,more than 100 genome regions associated with resistance are located,and 12 resistance-related genes are identified.In order to promote the research of soybean disease resistance breeding,this paper expounded and analyzed the pathogenesis characteristics of soybean FLS,the division of races,the physiological and biochemical mechanism of soybean resistance to FLS disease,quantitative trait locus(QTL),quantitative trait nucleotides(QTN),genes of resistance sites,the screening of resistant germplasm resources,and the breeding of new varieties,so as to gain an in-depth understanding of the pathogenesis principle of soybean FLS disease.In order to provide a theoretical basis and technical support for the breeding of soybean FLS disease,the resistance mechanism of soybean FLS disease was analyzed from the molecular level.展开更多
In this study,the morphology and ultrastructure of the compound eye of L.lewisii were investigated using scanning electron microscopy(SEM),transmission electron microscopy(TEM),microcomputed tomography(μCT),and 3D re...In this study,the morphology and ultrastructure of the compound eye of L.lewisii were investigated using scanning electron microscopy(SEM),transmission electron microscopy(TEM),microcomputed tomography(μCT),and 3D reconstruction.The compound eye of L.lewisii was of the apposition type,with an average of 121.88±7.64 ommatidia in males and 119.00±4.71 ommatidia in females.Each ommatidium was composed of a biconvex cornea,an acone consisting of four cone cells,eight retinular cells along with the rhabdom,two primary pigment cells,and numerous secondary pigment cells.The open type of rhabdom in L.lewisii consists of six peripheral rhabdomeres contributed by the six peripheral retinular cells(R1~R6)and two vertically attached central rhabdomeres contributed by R7 and R8 respectively.The orientation of microvilli suggested a weak sensitivity to polarized light perception.展开更多
Background Natural and synthetic plant growth regulators are essential for plant health,likewise these regulators also play a role in increasing organic production productivity and improving quality and yield stabilit...Background Natural and synthetic plant growth regulators are essential for plant health,likewise these regulators also play a role in increasing organic production productivity and improving quality and yield stability.In the present study,we have evaluated the effects of foliar applied plant growth regulators,i.e.,moringa leaf extract(MLE)and mepiquat chloride(MC)alone and in combination MC and MLE on the conventional cotton cultivar(CIM 573)and transgenic one(CIM 598).The growth regulators were applied at the start of bloom,45 and 90 days after blooming.Results The application of MC and MLE at 90 days after blooming significantly improved the relative growth rate,net assimilation rate,the number of bolls per plant,and seed cotton yield.Likewise,the combined application of MLE and MC at 90 days after blooming significantly boosted the nitrogen uptake in locules,as well as the phosphorus and potassium uptake in the leaves of both cotton cultivars.The application of MLE alone has considerably improved the nitrogen uptake in leaves,and phosphorus and potassium contents in locules of Bt and conventional cotton cultivars.Similarly,Bt cotton treated with MLE at 90 days after blooming produced significantly higher ginning out turn and oil contents.Treatment in combination(MLE+MC)at 90 days after blooming produced considerably higher micronaire value,fiber strength,and staple length in conventional cultivar.Conclusion The natural growth enhancer,MLE is a rich source of minerals and zeatin,improving the nutrient absorption and quality of cotton fiber in both conventional and Bt cotton cultivars.展开更多
Soybean frogeye leaf spot(FLS) disease is a global disease affecting soybean yield, especially in the soybean growing area of Heilongjiang Province. In order to realize genomic selection breeding for FLS resistance of...Soybean frogeye leaf spot(FLS) disease is a global disease affecting soybean yield, especially in the soybean growing area of Heilongjiang Province. In order to realize genomic selection breeding for FLS resistance of soybean, least absolute shrinkage and selection operator(LASSO) regression and stepwise regression were combined, and a genomic selection model was established for 40 002 SNP markers covering soybean genome and relative lesion area of soybean FLS. As a result, 68 molecular markers controlling soybean FLS were detected accurately, and the phenotypic contribution rate of these markers reached 82.45%. In this study, a model was established, which could be used directly to evaluate the resistance of soybean FLS and to select excellent offspring. This research method could also provide ideas and methods for other plants to breeding in disease resistance.展开更多
草莓角斑病（Angular leaf spot of strawberry）是草莓生产上的主要病害，其病原菌为黄单孢杆菌属草莓黄单孢菌，属细菌草莓角斑病菌。1962年首次报道于北美洲，草莓角斑病会引起产量下降，但一般不是毁灭性的。已有该病害发生的国家...草莓角斑病（Angular leaf spot of strawberry）是草莓生产上的主要病害，其病原菌为黄单孢杆菌属草莓黄单孢菌，属细菌草莓角斑病菌。1962年首次报道于北美洲，草莓角斑病会引起产量下降，但一般不是毁灭性的。已有该病害发生的国家包括：欧洲的法国、希腊、意大利、葡萄牙、罗马尼亚、西班牙；非洲的留尼汪；美洲的美国、阿根廷、巴西、委内瑞拉；展开更多
文摘The anti-hair loss mechanism of Aquilaria sinensis leaf extract(ASE)has been studied by using metabolomics and network pharmacology.Metabolomics was utilized to comprehensively identify the active constituents of ASE,and the network pharmacology was used to elucidate their anti-hair loss mechanism,which was verified by molecular docking technology.572 active compounds were identified from the ASE by metabolomics methods,where there are 1447 corresponding targets and 492 targets related to hair loss,totaling 88 targets.20 core active substances were identified by constructing a network between common targets and active substances,which include vanillic acid,chorionic acid,caffeic acid and apigenin.The five key targets of TNF,TP53,IL6,PPARG,and EGFR were screened out by the PPI network analysis on 88 common targets.The GO and KEGG pathway enrichment analysis showed that the inflammation,hormone balance,cell growth,proliferation,apoptosis,and oxidative stress are involved.Molecular docking studies have confirmed the high binding affinity between core active compounds and key targets.The drug similarity assessment on these core compounds suggested that they have the potential to be used as potential hair loss treatment drugs.This study elucidates the complex molecular mechanism of ASE in treating hair loss,and provides a reference for the future applications in hair care products.
基金Supported by the 14th Five-Year National Key Research and Development Program(2021YFD1201103–01–05)the National Natural Science Foundation of China(32301819)the Cooperation Project of Research and Development Center between Wudalianchi Government and Northeast Agricultural University.
文摘Soybean frogeye leaf spot(FLS)disease is a worldwide disease caused by Cercospora sojina Hara.It is one of the major diseases suffered by soybean during the growth cycle,which seriously damages the yield and seed quality of soybean.The current resistant varieties are difficult to meet the production demand.The breeders have identified 50 different physiological small species and discussed the physiological and biochemical characteristics of soybean resistance to FLS.In soybean disease resistance breeding,resistance resources are screened for the main physiological races in different countries,resistance materials are created,more than 100 genome regions associated with resistance are located,and 12 resistance-related genes are identified.In order to promote the research of soybean disease resistance breeding,this paper expounded and analyzed the pathogenesis characteristics of soybean FLS,the division of races,the physiological and biochemical mechanism of soybean resistance to FLS disease,quantitative trait locus(QTL),quantitative trait nucleotides(QTN),genes of resistance sites,the screening of resistant germplasm resources,and the breeding of new varieties,so as to gain an in-depth understanding of the pathogenesis principle of soybean FLS disease.In order to provide a theoretical basis and technical support for the breeding of soybean FLS disease,the resistance mechanism of soybean FLS disease was analyzed from the molecular level.
文摘In this study,the morphology and ultrastructure of the compound eye of L.lewisii were investigated using scanning electron microscopy(SEM),transmission electron microscopy(TEM),microcomputed tomography(μCT),and 3D reconstruction.The compound eye of L.lewisii was of the apposition type,with an average of 121.88±7.64 ommatidia in males and 119.00±4.71 ommatidia in females.Each ommatidium was composed of a biconvex cornea,an acone consisting of four cone cells,eight retinular cells along with the rhabdom,two primary pigment cells,and numerous secondary pigment cells.The open type of rhabdom in L.lewisii consists of six peripheral rhabdomeres contributed by the six peripheral retinular cells(R1~R6)and two vertically attached central rhabdomeres contributed by R7 and R8 respectively.The orientation of microvilli suggested a weak sensitivity to polarized light perception.
文摘Background Natural and synthetic plant growth regulators are essential for plant health,likewise these regulators also play a role in increasing organic production productivity and improving quality and yield stability.In the present study,we have evaluated the effects of foliar applied plant growth regulators,i.e.,moringa leaf extract(MLE)and mepiquat chloride(MC)alone and in combination MC and MLE on the conventional cotton cultivar(CIM 573)and transgenic one(CIM 598).The growth regulators were applied at the start of bloom,45 and 90 days after blooming.Results The application of MC and MLE at 90 days after blooming significantly improved the relative growth rate,net assimilation rate,the number of bolls per plant,and seed cotton yield.Likewise,the combined application of MLE and MC at 90 days after blooming significantly boosted the nitrogen uptake in locules,as well as the phosphorus and potassium uptake in the leaves of both cotton cultivars.The application of MLE alone has considerably improved the nitrogen uptake in leaves,and phosphorus and potassium contents in locules of Bt and conventional cotton cultivars.Similarly,Bt cotton treated with MLE at 90 days after blooming produced significantly higher ginning out turn and oil contents.Treatment in combination(MLE+MC)at 90 days after blooming produced considerably higher micronaire value,fiber strength,and staple length in conventional cultivar.Conclusion The natural growth enhancer,MLE is a rich source of minerals and zeatin,improving the nutrient absorption and quality of cotton fiber in both conventional and Bt cotton cultivars.
基金Supported by the National Key Research and Development Program of China(2021YFD1201103-01-05)。
文摘Soybean frogeye leaf spot(FLS) disease is a global disease affecting soybean yield, especially in the soybean growing area of Heilongjiang Province. In order to realize genomic selection breeding for FLS resistance of soybean, least absolute shrinkage and selection operator(LASSO) regression and stepwise regression were combined, and a genomic selection model was established for 40 002 SNP markers covering soybean genome and relative lesion area of soybean FLS. As a result, 68 molecular markers controlling soybean FLS were detected accurately, and the phenotypic contribution rate of these markers reached 82.45%. In this study, a model was established, which could be used directly to evaluate the resistance of soybean FLS and to select excellent offspring. This research method could also provide ideas and methods for other plants to breeding in disease resistance.
文摘草莓角斑病（Angular leaf spot of strawberry）是草莓生产上的主要病害，其病原菌为黄单孢杆菌属草莓黄单孢菌，属细菌草莓角斑病菌。1962年首次报道于北美洲，草莓角斑病会引起产量下降，但一般不是毁灭性的。已有该病害发生的国家包括：欧洲的法国、希腊、意大利、葡萄牙、罗马尼亚、西班牙；非洲的留尼汪；美洲的美国、阿根廷、巴西、委内瑞拉；
