The plant pathogen Verticillium dahliae causes severe cotton losses in Uzbekistan. To create cotton varieties that are resistant to the more virulent races of V.dahliae we wanted to determine
In this study, the three-dimensional non-premixed two-phase kerosene/air rotating detonation engines with different isolator configurations and throat area ratios are simulated by the Eulerian-Lagrangian method. The e...In this study, the three-dimensional non-premixed two-phase kerosene/air rotating detonation engines with different isolator configurations and throat area ratios are simulated by the Eulerian-Lagrangian method. The effects of the divergence, straight, and convergence isolators on the rotating detonation wave dynamics and the upstream oblique shock wave propagation mechanism are analyzed. The differences in the rotating detonation wave behaviors between ground and flight operations are clarified.The results indicate that the propagation regimes of the upstream oblique shock wave depend on the isolator configurations and operation conditions. With a divergence isolator, the airflow is accelerated throughout the isolator and divergence section, leading to a maximum Mach number(~1.8) before the normal shock. The total pressure loss reaches the largest, and the detonation pressure drops. The upstream oblique shock wave can be suppressed within the divergence section with the divergence isolator.However, for the straight and convergence isolators, the airflow in the isolator with a larger ψ_(1)(0.3 and0.4) can suffer from the disturbance of the upstream oblique shock wave. The critical incident angle is around 39° at ground operation conditions. The upstream oblique shock wave tends to be suppressed when the engine operates under flight operation conditions. The critical pressure ratio β_(cr0) is found to be able to help in distinguishing the propagation regimes of the upstream oblique shock wave. Slightly below or above the β_(cr0) can obtain different marginal propagation results. The high-speed airflow in the divergence section affects the fuel droplet penetration distance, which deteriorates the reactant mixing and the detonation area. Significant detonation velocity deficits are observed and the maximum velocity deficit reaches 26%. The results indicate the engine channel design should adopt different isolator configurations based on the purpose of total pressure loss or disturbance suppression. This study can provide useful guidance for the channel design of a more complete two-phase rotating detonation engine.展开更多
Background Cotton is an important crop providing the most natural fibers all over the world. The cotton genomics community has utilized whole genome sequencing data to construct an elite gene pool in which functional ...Background Cotton is an important crop providing the most natural fibers all over the world. The cotton genomics community has utilized whole genome sequencing data to construct an elite gene pool in which functional genes are related to agronomic traits. However, the functional validation of these genes is hindered by time-consuming and inefficient genetic transformation methods. Thus, establishing a transient transformation system of high efficiency is necessary for cotton genomics.Results To improve the efficiency of transient transformation, we used the protoplasts isolated from the etiolated cotyledon as recipient. The enzymatic digestion buffer comprised 1.5%(w/v) cellulase, 0.75%(w/v) macerozyme, and 1% hemicellulase, osmotically buffered with 0.4 mol·L^(-1) mannitol. After 5 h of dark incubation at 25℃, uniform cotton protoplasts were successfully isolated with a yield of 4.6 × 10^(6) protoplasts per gram(fresh weight) and 95% viability. We incubated 100 μL protoplasts(2.5 × 10^(5)·m L^(-1)) with 15 μg plasmid in the solution of 0.4 mol·L^(-1) mannitol and 40% PEG 4000 for 15 min, ultimately achieving an optimal transient transfection efficiency of 71.47%.Conclusions This transient system demonstrated effective utility in cellular biology research through successful applications in subcellular localization analyses, bimolecular fluorescence complementation(Bi FC) verification, and prime editing vector validation. Through systematic optimization, we established an efficient and expedited protoplast-based transient transformation system and successfully applied this platform to cotton functional genomics studies.展开更多
The process of ground vehicle dynamic gravimetry is inevitably affected by the carrier’s maneuvering acceleration,which makes the result contain a large amount of dynamic error.In this paper,we propose a dynamic erro...The process of ground vehicle dynamic gravimetry is inevitably affected by the carrier’s maneuvering acceleration,which makes the result contain a large amount of dynamic error.In this paper,we propose a dynamic error suppression method of gravimetry based on the high-precision acquisition of external velocity for compensating the horizontal error of the inertial plat-form.On the basis of platform gravity measurement,firstly,the dynamic performance of the system is enhanced by optimizing the horizontal damping network of the inertial platform and selecting its parameter.Secondly,an improved federal Kalman filtering algorithm and a fault diagnosis method are designed using strapdown inertial navigation system(SINS),odometer(OD),and laser Doppler velocimeter(LDV).Simulation validates that these methods can improve the accuracy and robustness of the external velocity acquisition.Three survey lines are selected in Tianjin,China,for the gravimetry experiments with different maneuvering levels,and the results demonstrate that after dynamic error suppression,the internal coincidence accuracies of smooth and uniform operation,obvious acceleration and deceleration operation,and high-dynamic operation are improved by 70.2%,73.6%,and 77.9%to reach 0.81 mGal,1.30 mGal,and 1.94 mGal,respectively,and the external coinci-dence accuracies during smooth and uniform operation are improved by 48.6%up to 1.66 mGal.It is shown that the pro-posed method can effectively suppress the dynamic error,and that the accuracy improvement increases with carrier maneuver-ability.However,the amount of residual error that can not be entirely eliminated increases as well,so the ground vehicle dynamic gravimetry should be maintained in the carrier for smooth and uniform operation.展开更多
The problems associated with vibrations of viaducts and low-frequency structural noise radiation caused by train excitation continue to increase in importance.A new floating-slab track vibration isolator-non-obstructi...The problems associated with vibrations of viaducts and low-frequency structural noise radiation caused by train excitation continue to increase in importance.A new floating-slab track vibration isolator-non-obstructive particle damping-phononic crystal vibration isolator is proposed herein,which uses the particle damping vibration absorption technology and bandgap vibration control theory.The vibration reduction performance of the NOPD-PCVI was analyzed from the perspective of vibration control.The paper explores the structure-borne noise reduction performance of the NOPD-PCVIs installed on different bridge structures under varying service conditions encountered in practical engineering applications.The load transferred to the bridge is obtained from a coupled train-FST-bridge analytical model considering the different structural parameters of bridges.The vibration responses are obtained using the finite element method,while the structural noise radiation is simulated using the frequency-domain boundary element method.Using the particle swarm optimization algorithm,the parameters of the NOPD-PCVI are optimized so that its frequency bandgap matches the dominant bridge structural noise frequency range.The noise reduction performance of the NOPD-PCVIs is compared to the steel-spring isolation under different service conditions.展开更多
High-static-low-dynamic stiffness (HSLDS) vibration isolators have been demonstrated to be an effective means of attenuating low-frequency vibrations, and may be utilized for ship shafting applications to mitigate tor...High-static-low-dynamic stiffness (HSLDS) vibration isolators have been demonstrated to be an effective means of attenuating low-frequency vibrations, and may be utilized for ship shafting applications to mitigate torsional vibration. This paper presents the construction of a highly compact HSLDS torsional vibration isolator by connecting positive and negative stiffness components in paral lel. Based on mechanical model analysis, the restoring torque of negative stiffness components is de rived from their springs and connecting rods, while that of positive stiffness components is obtained through their circular section flexible rods. The quasizero stiffness characteristics of the HSLDS iso lator are achieved through a combination of static structural simulation and experimental test. The tor sional vibration isolation performance is assessed by means of numerical simulation and theory analy sis. Finally, the frequency-sweep vibration test is conducted. The test results indicate that the HSLDS torsional vibration isolator exhibits superior low-frequency isolation performance compared to its linear counterpart, rendering it a promising solution for mitigating low-frequency torsional vi bration in ship shafting.展开更多
Wastewater effluents containing phosphorus and nitrogen are major contributors to a variety of water pollution.The present study is therefore aimed at investigating the phosphate and nitrate removal efficiency of thre...Wastewater effluents containing phosphorus and nitrogen are major contributors to a variety of water pollution.The present study is therefore aimed at investigating the phosphate and nitrate removal efficiency of three wastewater protozoa(Aspidisca, Trachelophyllum and Peranema) in a laboratory-scale water treatment system.The system was made of two separate reactors(aerated and non-aerated) and展开更多
The differential cubature solution to the problem of a Mindlin plate lying on the Winkler foundation with two simply supported edges and two clamped edges was derived.Discrete numerical technology and shape functions ...The differential cubature solution to the problem of a Mindlin plate lying on the Winkler foundation with two simply supported edges and two clamped edges was derived.Discrete numerical technology and shape functions were used to ensure that the solution is suitable to irregular shaped plates.Then,the mechanical model and the solution were employed to model the protection layer that isolates the mining stopes from sea water in Sanshandao gold mine,which is the first subsea mine of China.Furthermore,thickness optimizations for the protection layers above each stope were conducted based on the maximum principle stress criterion,and the linear relations between the best protection layer thickness and the stope area under different safety factors were regressed to guide the isolation design.The method presented in this work provides a practical way to quickly design the isolation layer thickness in subsea mining.展开更多
According to physiological and biochemical characteristics of Leptospirillum ferriphilum, a strain of object bacteria was isolated successfully. Bacteria were enriched by selective liquid medium and plated on designed...According to physiological and biochemical characteristics of Leptospirillum ferriphilum, a strain of object bacteria was isolated successfully. Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium. Colony was cultured and bacteria were collected. The morphologies of the object bacteria were observed using crystal violet staining, scanning electron microscope（SEM） and transmission electron microscope （TEM）. The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1. These results indicate that this new single-layered agar solid medium is efficient and physiological-biochemical characteristics show that the optimum simple for isolation of Leptospirillum ferriphilum. Additionally, initial pH value and its growth temperature are 1.68 and 40℃.展开更多
A robust fault diagnosis approach is developed by incorporating a set-membership identification (SMI) method. A class of systems with linear models in the form of fault related parameters is investigated, with model u...A robust fault diagnosis approach is developed by incorporating a set-membership identification (SMI) method. A class of systems with linear models in the form of fault related parameters is investigated, with model uncertainties and parameter variations taken into account explicitly and treated as bounded errors. An ellipsoid bounding set-membership identification algorithm is proposed to propagate bounded uncertainties rigorously and the guaranteed feasible set of faults parameters enveloping true parameter values is given. Faults arised from abrupt parameter variations can be detected and isolated on-line by consistency check between predicted and observed parameter sets obtained in the identification procedure. The proposed approach provides the improved robustness with its ability to distinguish real faults from model uncertainties, which comes with the inherent guaranteed robustness of the set-membership framework. Efforts are also made in this work to balance between conservativeness and computation complexity of the overall algorithm. Simulation results for the mobile robot with several slipping faults scenarios demonstrate the correctness of the proposed approach for faults detection and isolation (FDI).展开更多
The boundary between the near and far fields is generally defined as the distance from the vibration source beyond which ground vibrations are mainly dominated by Rayleigh waves. It is closely related to the type of v...The boundary between the near and far fields is generally defined as the distance from the vibration source beyond which ground vibrations are mainly dominated by Rayleigh waves. It is closely related to the type of vibration source and the soil properties. Based on the solutions of the Lamb＇s problem, the boundary at the surface between the near and far fields of ground vibration was investigated for a harmonic vertical concentrated load and an infinite line load at the surface of a visco-elastic half-space. Particularly, the variation of the boundary with the material damping was investigated for both cases. The results indicate that the material damping slightly contributes to the attenuation of vibrations in the near-source region, but significantly reduces the vibrations in the region that is at some distance away from the source. When taking the material damping into consideration, the boundary between the near and far fields tends to move towards the vibration source. Compared with the vibrations caused by a concentrated load, the vibrations induced by an infinite line load can affect a larger range of the surrounding environment, and they attenuate more slowly. This means the boundary between the near field and far field should move fitrther away from the source. Finally, the boundaries are defined in terms of R-wave length （2R） and Poisson ratio of the ground （o）. For the case of a point load, the boundary is located at the distance of （5.0-6.0）2R for v≤0.30 and at the distance of （2.0--3.0）2R for v≥0.35. For the case of an infinite line load, the boundary is located at the distance （5.5-6.5）2rt for v≤0.30 and at the distance （2.5--3.5）2R for v≥0.35.展开更多
The micromation and precision of the Micro-Electromechanical System demand that its manufacturing, measuring and assembling must work in a micro-manufacturing platform with good ability to isolate vibrations. This pap...The micromation and precision of the Micro-Electromechanical System demand that its manufacturing, measuring and assembling must work in a micro-manufacturing platform with good ability to isolate vibrations. This paper develops a vibration isolation system of micro-manufacturing platform. The brains of many kinds of birds can isolate vibrations well, such as woodpecker’s brain. When a woodpecker pecks the wood at the speed as 1.6 times as the velocity of sound, its brain will tolerate the wallop 1 500 times of the weight of itself without any damage. The isolation mechanics and organic texture of woodpecker’s brain that has good isolation characteristics were studied. A structure model of vibration isolation system for the micro-manufacturing platform is established based on the bionics of the bird’s brain vibration isolation mechanism. In order to isolate effectively the high frequency vibrations from the ground, a rubber layer is used to isolate vibrations passively between the micro-manufacturing platform’s pedestal and the ground. This layer corresponds to the cartilage and muscles in the outer meninges of the bird’s brain. The active vibration isolation technique is adopted to isolate vibrations between the micro-manufacturing platform and the pedestal. Air springs are used as elastic components, which correspond to the interspaces between the outer meninges and the encephala of the bird’s brain. Actuators are made of giant magnetostrictive material, and it corresponds to the nerves and neural muscles linking the meninges and the encephala. The actuators and air springs are arranged vertically in parallel to make use of the giant magnetostrictive actuators effectively. The air springs support almost all weight of the micro-manufacturing platform and the giant magnetostrictive actuators support almost no weight. In order to realize high performance to isolate complex micro-vibration, the control method using a three-layer neural network is presented. This vibration control system takes into account the floor disturbance and the direct disturbance acting on the micro-manufacturing platform. The absolute acceleration of the micro-manufacturing platform is used as the performance index of vibration control. The performance of the control system is tested by numerical simulation. Simulation results show that the active vibration isolation system has good isolation performance against the floor disturbance and the direct disturbance acting on the micro-manufacturing platform in all the frequency range.展开更多
Lonicera caerulea L.fruit is an excellent source of bioactive compounds.An efficient separation method of cyanins is important for the development of many value-added products and functional food ingredients.High-spee...Lonicera caerulea L.fruit is an excellent source of bioactive compounds.An efficient separation method of cyanins is important for the development of many value-added products and functional food ingredients.High-speed counter-current chromatography(HSCCC) was applied to isolate cyanins from Lonicera caerulea fruits with a biphasic solvent system composed of methyl tert-butyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid(2:2:1:5:0.01,volume ratio).1.41 mg of cyanidin 3,5-O-diglucoside,1.08 mg of cyanidin 3-O-rutinoside and 6.38 mg of cyanidin 3-O-glucoside were obtained from 40 mg of crude extract.The purities of these compounds were 95.8%,92.4% and 97.6%,respectively,as identified by high-performance liquid chromatography–diode array detection(HPLC-DAD) and high-performance liquid chromatography-electrospray ionization mass spectrometryn(HPLC-ESI/MSn).In addition,the dominant anthocyanin,cyanidin 3-O-glucoside was demonstrated cytotoxic response of human hepatocarcinoma SMMC-7721 cells,inducing live cancer cell apoptotic by flow cytometric analysis.展开更多
Collimating lenses are an important component of laser ranging systems,and high overload environments severely affect the beam shaping effect of such lenses.This study proposes a buffer isolation method on the basis o...Collimating lenses are an important component of laser ranging systems,and high overload environments severely affect the beam shaping effect of such lenses.This study proposes a buffer isolation method on the basis of impact stress wave attenuation theory for collimating lens spacers,ANSYS finite element software was applied to simulate the high load dynamics of collimating lenses,and the buffer isolation performance of different materials and composite structures was compared and analysed.Optical simulation analysis of the collimating lenses under different buffer conditions was performed using ZEMAX software to study the mechanism by which a high overload affects the collimating lenses.High overload and optical shaping experiments based on theoretical analysis were further conducted.Results showed the superiority of butadiene rubber to polytetrafluoroethylene after application of 70000 g impact acceleration.The combination-gasket method was superior to the single-gasket method,and the sandwich combination-gasket method was superior to the double-layer combination-gasket method.展开更多
文摘The plant pathogen Verticillium dahliae causes severe cotton losses in Uzbekistan. To create cotton varieties that are resistant to the more virulent races of V.dahliae we wanted to determine
基金supported by the National Natural Science Foundation of China (Grant No. 12202204)the Natural Science Foundation of Jiangsu Province (Grant No. BK20220953)+1 种基金the Fundamental Research Funds for the Central Universitiesthe Science and Technology Association's Young Talent Nurturing Program of Jiangsu Province (Grant No. JSTJ-2024-004)
文摘In this study, the three-dimensional non-premixed two-phase kerosene/air rotating detonation engines with different isolator configurations and throat area ratios are simulated by the Eulerian-Lagrangian method. The effects of the divergence, straight, and convergence isolators on the rotating detonation wave dynamics and the upstream oblique shock wave propagation mechanism are analyzed. The differences in the rotating detonation wave behaviors between ground and flight operations are clarified.The results indicate that the propagation regimes of the upstream oblique shock wave depend on the isolator configurations and operation conditions. With a divergence isolator, the airflow is accelerated throughout the isolator and divergence section, leading to a maximum Mach number(~1.8) before the normal shock. The total pressure loss reaches the largest, and the detonation pressure drops. The upstream oblique shock wave can be suppressed within the divergence section with the divergence isolator.However, for the straight and convergence isolators, the airflow in the isolator with a larger ψ_(1)(0.3 and0.4) can suffer from the disturbance of the upstream oblique shock wave. The critical incident angle is around 39° at ground operation conditions. The upstream oblique shock wave tends to be suppressed when the engine operates under flight operation conditions. The critical pressure ratio β_(cr0) is found to be able to help in distinguishing the propagation regimes of the upstream oblique shock wave. Slightly below or above the β_(cr0) can obtain different marginal propagation results. The high-speed airflow in the divergence section affects the fuel droplet penetration distance, which deteriorates the reactant mixing and the detonation area. Significant detonation velocity deficits are observed and the maximum velocity deficit reaches 26%. The results indicate the engine channel design should adopt different isolator configurations based on the purpose of total pressure loss or disturbance suppression. This study can provide useful guidance for the channel design of a more complete two-phase rotating detonation engine.
基金supported by Biological Breeding of Early Maturing and Disease Resistant Cotton Varieties (NO.2023ZD04041)the Project of China Agriculture Research System (Grant No. CARS-15-06)+2 种基金Natural Science Foundation of Henan Province (Grant No. 232300421041 and 222300420382)National Natural Science Foundation of China (Grant No. U21 A20213)the Central Public-interest Scientific Institution Basal Research Fund (Grant No. 1610162023017 and 1610162023028)。
文摘Background Cotton is an important crop providing the most natural fibers all over the world. The cotton genomics community has utilized whole genome sequencing data to construct an elite gene pool in which functional genes are related to agronomic traits. However, the functional validation of these genes is hindered by time-consuming and inefficient genetic transformation methods. Thus, establishing a transient transformation system of high efficiency is necessary for cotton genomics.Results To improve the efficiency of transient transformation, we used the protoplasts isolated from the etiolated cotyledon as recipient. The enzymatic digestion buffer comprised 1.5%(w/v) cellulase, 0.75%(w/v) macerozyme, and 1% hemicellulase, osmotically buffered with 0.4 mol·L^(-1) mannitol. After 5 h of dark incubation at 25℃, uniform cotton protoplasts were successfully isolated with a yield of 4.6 × 10^(6) protoplasts per gram(fresh weight) and 95% viability. We incubated 100 μL protoplasts(2.5 × 10^(5)·m L^(-1)) with 15 μg plasmid in the solution of 0.4 mol·L^(-1) mannitol and 40% PEG 4000 for 15 min, ultimately achieving an optimal transient transfection efficiency of 71.47%.Conclusions This transient system demonstrated effective utility in cellular biology research through successful applications in subcellular localization analyses, bimolecular fluorescence complementation(Bi FC) verification, and prime editing vector validation. Through systematic optimization, we established an efficient and expedited protoplast-based transient transformation system and successfully applied this platform to cotton functional genomics studies.
基金supported by the Shanxi Provincial Natural Science Basic Research Program Young Talent Project(S2019-JC-QN-2408).
文摘The process of ground vehicle dynamic gravimetry is inevitably affected by the carrier’s maneuvering acceleration,which makes the result contain a large amount of dynamic error.In this paper,we propose a dynamic error suppression method of gravimetry based on the high-precision acquisition of external velocity for compensating the horizontal error of the inertial plat-form.On the basis of platform gravity measurement,firstly,the dynamic performance of the system is enhanced by optimizing the horizontal damping network of the inertial platform and selecting its parameter.Secondly,an improved federal Kalman filtering algorithm and a fault diagnosis method are designed using strapdown inertial navigation system(SINS),odometer(OD),and laser Doppler velocimeter(LDV).Simulation validates that these methods can improve the accuracy and robustness of the external velocity acquisition.Three survey lines are selected in Tianjin,China,for the gravimetry experiments with different maneuvering levels,and the results demonstrate that after dynamic error suppression,the internal coincidence accuracies of smooth and uniform operation,obvious acceleration and deceleration operation,and high-dynamic operation are improved by 70.2%,73.6%,and 77.9%to reach 0.81 mGal,1.30 mGal,and 1.94 mGal,respectively,and the external coinci-dence accuracies during smooth and uniform operation are improved by 48.6%up to 1.66 mGal.It is shown that the pro-posed method can effectively suppress the dynamic error,and that the accuracy improvement increases with carrier maneuver-ability.However,the amount of residual error that can not be entirely eliminated increases as well,so the ground vehicle dynamic gravimetry should be maintained in the carrier for smooth and uniform operation.
基金Project(51978585)supported by the National Natural Science Foundation,ChinaProject(2022YFB2603404)supported by the National Key Research and Development Program,China+1 种基金Project(U1734207)supported by the High-speed Rail Joint Fund Key Projects of Basic Research,ChinaProject(2023NSFSC1975)supported by the Sichuan Nature and Science Foundation Innovation Research Group Project,China。
文摘The problems associated with vibrations of viaducts and low-frequency structural noise radiation caused by train excitation continue to increase in importance.A new floating-slab track vibration isolator-non-obstructive particle damping-phononic crystal vibration isolator is proposed herein,which uses the particle damping vibration absorption technology and bandgap vibration control theory.The vibration reduction performance of the NOPD-PCVI was analyzed from the perspective of vibration control.The paper explores the structure-borne noise reduction performance of the NOPD-PCVIs installed on different bridge structures under varying service conditions encountered in practical engineering applications.The load transferred to the bridge is obtained from a coupled train-FST-bridge analytical model considering the different structural parameters of bridges.The vibration responses are obtained using the finite element method,while the structural noise radiation is simulated using the frequency-domain boundary element method.Using the particle swarm optimization algorithm,the parameters of the NOPD-PCVI are optimized so that its frequency bandgap matches the dominant bridge structural noise frequency range.The noise reduction performance of the NOPD-PCVIs is compared to the steel-spring isolation under different service conditions.
文摘High-static-low-dynamic stiffness (HSLDS) vibration isolators have been demonstrated to be an effective means of attenuating low-frequency vibrations, and may be utilized for ship shafting applications to mitigate torsional vibration. This paper presents the construction of a highly compact HSLDS torsional vibration isolator by connecting positive and negative stiffness components in paral lel. Based on mechanical model analysis, the restoring torque of negative stiffness components is de rived from their springs and connecting rods, while that of positive stiffness components is obtained through their circular section flexible rods. The quasizero stiffness characteristics of the HSLDS iso lator are achieved through a combination of static structural simulation and experimental test. The tor sional vibration isolation performance is assessed by means of numerical simulation and theory analy sis. Finally, the frequency-sweep vibration test is conducted. The test results indicate that the HSLDS torsional vibration isolator exhibits superior low-frequency isolation performance compared to its linear counterpart, rendering it a promising solution for mitigating low-frequency torsional vi bration in ship shafting.
文摘利用FIASCO(Fast Isolation by AFLP Sequences Containing repeats)技术建立拟穴青蟹Scylla paramamosain基因组文库,并与生物素标记的(CA)15寡核苷酸探针杂交,联合磁珠富集法构建拟穴青蟹微卫星富集文库。测序194个阳性菌落,分析其中的150条序列,结果表明:两碱基重复类型占90%以上,其中重复拷贝数在30以上的占27.45%;含微卫星座位189个,其中完美型146个、非完美型28个和复合型15个。设计125对引物扩增一个拟穴青蟹野生群体(含20个个体),其中的19对引物能稳定扩增且片段大小基本符合理论长度。遗传变异分析表明,17个位点表现出高度多态性,16个位点显著偏离Hardy-Weinberg平衡(P<0.05),4组两两位点间存在连锁不平衡现象(P<0.0026,经Bonferroni法校正),7个微卫星位点可能存在无效等位基因。若排除混合微卫星位点的引物对以及扩增位点PIC(polymorphism information content)值在0.5以下的引物对,则13对引物能用于拟穴青蟹群体遗传学等研究。
文摘Wastewater effluents containing phosphorus and nitrogen are major contributors to a variety of water pollution.The present study is therefore aimed at investigating the phosphate and nitrate removal efficiency of three wastewater protozoa(Aspidisca, Trachelophyllum and Peranema) in a laboratory-scale water treatment system.The system was made of two separate reactors(aerated and non-aerated) and
基金Projects(51504044,51204100)supported by National Natural Science Foundation of ChinaProject(14KF05)supported by the Research Fund of The State Key Laboratory of Coal Resources and Mine Safety,CUMT,China+3 种基金Project(cstc2016jcyj A1861)supported by the Research Fund of Chongqing Basic Science and Cutting-Edge Technology Special Projects,ChinaProject(2015CDJXY)supported by the Fundamental Research Funds for the Central UniversitiesProject supported by the China Postdoctoral Science FoundationProject(2011DA105287-MS201503)supported by the Independent Subject of State Key Laboratory of Coal Mine Disaster Dynamics and Control,China
文摘The differential cubature solution to the problem of a Mindlin plate lying on the Winkler foundation with two simply supported edges and two clamped edges was derived.Discrete numerical technology and shape functions were used to ensure that the solution is suitable to irregular shaped plates.Then,the mechanical model and the solution were employed to model the protection layer that isolates the mining stopes from sea water in Sanshandao gold mine,which is the first subsea mine of China.Furthermore,thickness optimizations for the protection layers above each stope were conducted based on the maximum principle stress criterion,and the linear relations between the best protection layer thickness and the stope area under different safety factors were regressed to guide the isolation design.The method presented in this work provides a practical way to quickly design the isolation layer thickness in subsea mining.
基金Projects(50374075, 50321402) supported by the National Natural Science Foundation of ChinaProject(2004CB619204) supported by the National Basic Research and Development Program of China+1 种基金Project(200549) supported by the Specialized Research Fund of the NationalExcellent PhD Thesis
文摘According to physiological and biochemical characteristics of Leptospirillum ferriphilum, a strain of object bacteria was isolated successfully. Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium. Colony was cultured and bacteria were collected. The morphologies of the object bacteria were observed using crystal violet staining, scanning electron microscope（SEM） and transmission electron microscope （TEM）. The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1. These results indicate that this new single-layered agar solid medium is efficient and physiological-biochemical characteristics show that the optimum simple for isolation of Leptospirillum ferriphilum. Additionally, initial pH value and its growth temperature are 1.68 and 40℃.
基金supported by the National Natural Science Foundation of China(616732546157310061573101)
文摘A robust fault diagnosis approach is developed by incorporating a set-membership identification (SMI) method. A class of systems with linear models in the form of fault related parameters is investigated, with model uncertainties and parameter variations taken into account explicitly and treated as bounded errors. An ellipsoid bounding set-membership identification algorithm is proposed to propagate bounded uncertainties rigorously and the guaranteed feasible set of faults parameters enveloping true parameter values is given. Faults arised from abrupt parameter variations can be detected and isolated on-line by consistency check between predicted and observed parameter sets obtained in the identification procedure. The proposed approach provides the improved robustness with its ability to distinguish real faults from model uncertainties, which comes with the inherent guaranteed robustness of the set-membership framework. Efforts are also made in this work to balance between conservativeness and computation complexity of the overall algorithm. Simulation results for the mobile robot with several slipping faults scenarios demonstrate the correctness of the proposed approach for faults detection and isolation (FDI).
基金Project(51178342)supported by the National Natural Science Foundation of ChinaProject(KLE-TJGE-C1301)supported by the Key Laboratory of Geotechnical and Underground Engineering of Ministry of Education(Tongji University)under the International Cooperation and Exchange Program,China
文摘The boundary between the near and far fields is generally defined as the distance from the vibration source beyond which ground vibrations are mainly dominated by Rayleigh waves. It is closely related to the type of vibration source and the soil properties. Based on the solutions of the Lamb＇s problem, the boundary at the surface between the near and far fields of ground vibration was investigated for a harmonic vertical concentrated load and an infinite line load at the surface of a visco-elastic half-space. Particularly, the variation of the boundary with the material damping was investigated for both cases. The results indicate that the material damping slightly contributes to the attenuation of vibrations in the near-source region, but significantly reduces the vibrations in the region that is at some distance away from the source. When taking the material damping into consideration, the boundary between the near and far fields tends to move towards the vibration source. Compared with the vibrations caused by a concentrated load, the vibrations induced by an infinite line load can affect a larger range of the surrounding environment, and they attenuate more slowly. This means the boundary between the near field and far field should move fitrther away from the source. Finally, the boundaries are defined in terms of R-wave length （2R） and Poisson ratio of the ground （o）. For the case of a point load, the boundary is located at the distance of （5.0-6.0）2R for v≤0.30 and at the distance of （2.0--3.0）2R for v≥0.35. For the case of an infinite line load, the boundary is located at the distance （5.5-6.5）2rt for v≤0.30 and at the distance （2.5--3.5）2R for v≥0.35.
文摘The micromation and precision of the Micro-Electromechanical System demand that its manufacturing, measuring and assembling must work in a micro-manufacturing platform with good ability to isolate vibrations. This paper develops a vibration isolation system of micro-manufacturing platform. The brains of many kinds of birds can isolate vibrations well, such as woodpecker’s brain. When a woodpecker pecks the wood at the speed as 1.6 times as the velocity of sound, its brain will tolerate the wallop 1 500 times of the weight of itself without any damage. The isolation mechanics and organic texture of woodpecker’s brain that has good isolation characteristics were studied. A structure model of vibration isolation system for the micro-manufacturing platform is established based on the bionics of the bird’s brain vibration isolation mechanism. In order to isolate effectively the high frequency vibrations from the ground, a rubber layer is used to isolate vibrations passively between the micro-manufacturing platform’s pedestal and the ground. This layer corresponds to the cartilage and muscles in the outer meninges of the bird’s brain. The active vibration isolation technique is adopted to isolate vibrations between the micro-manufacturing platform and the pedestal. Air springs are used as elastic components, which correspond to the interspaces between the outer meninges and the encephala of the bird’s brain. Actuators are made of giant magnetostrictive material, and it corresponds to the nerves and neural muscles linking the meninges and the encephala. The actuators and air springs are arranged vertically in parallel to make use of the giant magnetostrictive actuators effectively. The air springs support almost all weight of the micro-manufacturing platform and the giant magnetostrictive actuators support almost no weight. In order to realize high performance to isolate complex micro-vibration, the control method using a three-layer neural network is presented. This vibration control system takes into account the floor disturbance and the direct disturbance acting on the micro-manufacturing platform. The absolute acceleration of the micro-manufacturing platform is used as the performance index of vibration control. The performance of the control system is tested by numerical simulation. Simulation results show that the active vibration isolation system has good isolation performance against the floor disturbance and the direct disturbance acting on the micro-manufacturing platform in all the frequency range.
基金Project(KSCX2-YW-N-043)supported by the Knowledge Innovation Program of the Chinese Academy of Sciences,China
文摘Lonicera caerulea L.fruit is an excellent source of bioactive compounds.An efficient separation method of cyanins is important for the development of many value-added products and functional food ingredients.High-speed counter-current chromatography(HSCCC) was applied to isolate cyanins from Lonicera caerulea fruits with a biphasic solvent system composed of methyl tert-butyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid(2:2:1:5:0.01,volume ratio).1.41 mg of cyanidin 3,5-O-diglucoside,1.08 mg of cyanidin 3-O-rutinoside and 6.38 mg of cyanidin 3-O-glucoside were obtained from 40 mg of crude extract.The purities of these compounds were 95.8%,92.4% and 97.6%,respectively,as identified by high-performance liquid chromatography–diode array detection(HPLC-DAD) and high-performance liquid chromatography-electrospray ionization mass spectrometryn(HPLC-ESI/MSn).In addition,the dominant anthocyanin,cyanidin 3-O-glucoside was demonstrated cytotoxic response of human hepatocarcinoma SMMC-7721 cells,inducing live cancer cell apoptotic by flow cytometric analysis.
基金National Natural Science Foundation of China(51605227)the Fundamental Research Funds for the Central Universities(NUST30915011303)。
文摘Collimating lenses are an important component of laser ranging systems,and high overload environments severely affect the beam shaping effect of such lenses.This study proposes a buffer isolation method on the basis of impact stress wave attenuation theory for collimating lens spacers,ANSYS finite element software was applied to simulate the high load dynamics of collimating lenses,and the buffer isolation performance of different materials and composite structures was compared and analysed.Optical simulation analysis of the collimating lenses under different buffer conditions was performed using ZEMAX software to study the mechanism by which a high overload affects the collimating lenses.High overload and optical shaping experiments based on theoretical analysis were further conducted.Results showed the superiority of butadiene rubber to polytetrafluoroethylene after application of 70000 g impact acceleration.The combination-gasket method was superior to the single-gasket method,and the sandwich combination-gasket method was superior to the double-layer combination-gasket method.
