Based on chicken＇ consensus map issued in 2000, 17 microsatellites near 4 candidate genes such as IGF2, OBR, GDF8 and APOA 1 in 4 chromosomes （chromosome 5, 7, 8 and 24） were chosen for polymorphism analysis and co...Based on chicken＇ consensus map issued in 2000, 17 microsatellites near 4 candidate genes such as IGF2, OBR, GDF8 and APOA 1 in 4 chromosomes （chromosome 5, 7, 8 and 24） were chosen for polymorphism analysis and construction of linkage map. Combining the technique of PCR and the fluorescent semi-automated detection, genome scanning was performed for 440 chickens, which was derived from China Agricultural University chicken resource families within three generations. The individuals of this resource families were genotyped. The results showed that the number of alleles ranged from 4 to 14; heterozygosity （H） of markers was between 0.3116 and 0.9148. Polymorphic information content （PIC） varied from 0.2672 to 0.8679. Microsatellites along with above-mentioned 4 candidate genes doing as general markers were used to construct linkage map. The spans of 4 linkage maps constructed in the part region of chromosome 5, 7, 8 and 24 were 263.5, 79.9, 206.2 and 104.2 cM, respectively. The order of markers was consistent with that of counterpart of reported consensus map. However, The spans of linkage map were larger than that of consensus map. The constructed linkage maps laid the foundation for mapping quantitative trait loci （QTL） responsible for economically important traits in chicken.展开更多
Based on the distribution characteristic of magnetic field along the polish wheel,the four-axis linkage technique is advanced to replace a standard five-axis one to figure low-gradient optical surfaces with a raster t...Based on the distribution characteristic of magnetic field along the polish wheel,the four-axis linkage technique is advanced to replace a standard five-axis one to figure low-gradient optical surfaces with a raster tool-path in magnetorheological finishing(MRF).After introducing the fundaments of such simplification,the figuring reachability of a four-axis system for the low-gradient optics was theoretically analyzed.Further validation including magnetic field intensity and influence function characteristic was performed to establish its application.To demonstrate the correctness,feasibility and applicability of such technique,a K4 spherical part was figured by two iterations of MRF with surface form error improved to 0.219λPV and 0.027λRMS.Meanwhile,the surface roughness was also improved a lot in MRF process.These theoretical analyses and experimental results both indicate that high form accuracy and excellent surface quality can be obtained by using the four-axis linkage technique in the process of figuring low-gradient optical elements,and the four-axis linkage system undoubtedly is much more easy to control and much more economical.展开更多
In the modern business management,the role of corporate culture has been known to many enterprises. Moreover,the role of corporate culture in promoting corporate development has been increasingly significant. In the f...In the modern business management,the role of corporate culture has been known to many enterprises. Moreover,the role of corporate culture in promoting corporate development has been increasingly significant. In the formation of corporate culture,urban culture plays a profound influence. The linkage between urban culture and corporate culture has been focused in the minds of more and more people. In this paper,the ways for the linkage between urban culture and corporate culture are studied and the strategies for the construction of corporate culture are proposed.展开更多
Genetic linkage relationship of the natural colored fiber and six fuzzless seed germplasms in obsolete backgrounds of Gossypium hirsutum(AD genome) and G.barbadense were analyzed in the
Cotton is one of the most important economic crops in the world,and it provides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption demands on cotton fiber,b...Cotton is one of the most important economic crops in the world,and it provides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption demands on cotton fiber,both cotton yield and quality should be enhanced.However,cotton yield展开更多
Many cancer gene therapy strategies arevaccination strategies aiming at the induction ofcytolytic immune responses against the malignantcells. These strategies include modification of thetumour cells by transfection w...Many cancer gene therapy strategies arevaccination strategies aiming at the induction ofcytolytic immune responses against the malignantcells. These strategies include modification of thetumour cells by transfection with the genes of co-stimulatory molecules for cytotoxic T lymphocytes(CTL) such as IL-2 and B7, or manipulation ofbystander cells like autologous fibroblasts byintroduction of cytokine genes and subsequent co-展开更多
Primary germplasm pools represent the mostreadily accessible source of new alleles for cropimprovement,but when the most effectivealleles are not available in the primarygermplasm pool,breeders must confront thediffic...Primary germplasm pools represent the mostreadily accessible source of new alleles for cropimprovement,but when the most effectivealleles are not available in the primarygermplasm pool,breeders must confront thedifficulties associated with introgression展开更多
A permanent doubled haploid population fromthe crossing of G.hirsutum × G.barbadensewere developed by means of Vsg,virescentlymarked semigamy line in sea island cotton,which was characterized by a cytologicalmech...A permanent doubled haploid population fromthe crossing of G.hirsutum × G.barbadensewere developed by means of Vsg,virescentlymarked semigamy line in sea island cotton,which was characterized by a cytologicalmechanism for developing haploids with certainconvenience,and thus constructed展开更多
Molecular map construction is very important togene marking,map-based cloning and marker-assisted breeding.Some advance was made inthis area in the US(Shappley et al,1996;1998;Reinisch et al,1994),but little work was ...Molecular map construction is very important togene marking,map-based cloning and marker-assisted breeding.Some advance was made inthis area in the US(Shappley et al,1996;1998;Reinisch et al,1994),but little work was donein China.A preliminary molecular map withRFLP,RAPD and SSR was constructed andQTL maping was dong for some agronomic traitsby our research group.展开更多
CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used ...CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used to screen polymorphism among two parents,TM-1 and CSB14Sh,展开更多
基金Supported by Program for New Century Excellent Talents in University(NCET-04-0343)National Natural Science Foundation Key Project(30430510)Excellent Young Teachers Program of MOE.People's Republic of China(1985)
文摘Based on chicken＇ consensus map issued in 2000, 17 microsatellites near 4 candidate genes such as IGF2, OBR, GDF8 and APOA 1 in 4 chromosomes （chromosome 5, 7, 8 and 24） were chosen for polymorphism analysis and construction of linkage map. Combining the technique of PCR and the fluorescent semi-automated detection, genome scanning was performed for 440 chickens, which was derived from China Agricultural University chicken resource families within three generations. The individuals of this resource families were genotyped. The results showed that the number of alleles ranged from 4 to 14; heterozygosity （H） of markers was between 0.3116 and 0.9148. Polymorphic information content （PIC） varied from 0.2672 to 0.8679. Microsatellites along with above-mentioned 4 candidate genes doing as general markers were used to construct linkage map. The spans of 4 linkage maps constructed in the part region of chromosome 5, 7, 8 and 24 were 263.5, 79.9, 206.2 and 104.2 cM, respectively. The order of markers was consistent with that of counterpart of reported consensus map. However, The spans of linkage map were larger than that of consensus map. The constructed linkage maps laid the foundation for mapping quantitative trait loci （QTL） responsible for economically important traits in chicken.
基金Project(91023042)supported by the National Natural Science Foundation of ChinaProject(2011CB013200)supported by the National Basic Research Program of China+1 种基金Project(B090302)supported by the Fund of Innovation,Graduate School of National University of Defense Technology,ChinaProject(CX2009B004)supported by the Hunan Provincial Innovation Foundation for Postgraduate,China
文摘Based on the distribution characteristic of magnetic field along the polish wheel,the four-axis linkage technique is advanced to replace a standard five-axis one to figure low-gradient optical surfaces with a raster tool-path in magnetorheological finishing(MRF).After introducing the fundaments of such simplification,the figuring reachability of a four-axis system for the low-gradient optics was theoretically analyzed.Further validation including magnetic field intensity and influence function characteristic was performed to establish its application.To demonstrate the correctness,feasibility and applicability of such technique,a K4 spherical part was figured by two iterations of MRF with surface form error improved to 0.219λPV and 0.027λRMS.Meanwhile,the surface roughness was also improved a lot in MRF process.These theoretical analyses and experimental results both indicate that high form accuracy and excellent surface quality can be obtained by using the four-axis linkage technique in the process of figuring low-gradient optical elements,and the four-axis linkage system undoubtedly is much more easy to control and much more economical.
基金the Project of Shaanxi Federation of Social Science(No.2010C069)Study on Xi’an Religious Cultural Tourism(No.12IN27)
文摘In the modern business management,the role of corporate culture has been known to many enterprises. Moreover,the role of corporate culture in promoting corporate development has been increasingly significant. In the formation of corporate culture,urban culture plays a profound influence. The linkage between urban culture and corporate culture has been focused in the minds of more and more people. In this paper,the ways for the linkage between urban culture and corporate culture are studied and the strategies for the construction of corporate culture are proposed.
文摘Genetic linkage relationship of the natural colored fiber and six fuzzless seed germplasms in obsolete backgrounds of Gossypium hirsutum(AD genome) and G.barbadense were analyzed in the
文摘Cotton is one of the most important economic crops in the world,and it provides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption demands on cotton fiber,both cotton yield and quality should be enhanced.However,cotton yield
文摘Many cancer gene therapy strategies arevaccination strategies aiming at the induction ofcytolytic immune responses against the malignantcells. These strategies include modification of thetumour cells by transfection with the genes of co-stimulatory molecules for cytotoxic T lymphocytes(CTL) such as IL-2 and B7, or manipulation ofbystander cells like autologous fibroblasts byintroduction of cytokine genes and subsequent co-
文摘Primary germplasm pools represent the mostreadily accessible source of new alleles for cropimprovement,but when the most effectivealleles are not available in the primarygermplasm pool,breeders must confront thedifficulties associated with introgression
文摘A permanent doubled haploid population fromthe crossing of G.hirsutum × G.barbadensewere developed by means of Vsg,virescentlymarked semigamy line in sea island cotton,which was characterized by a cytologicalmechanism for developing haploids with certainconvenience,and thus constructed
文摘Molecular map construction is very important togene marking,map-based cloning and marker-assisted breeding.Some advance was made inthis area in the US(Shappley et al,1996;1998;Reinisch et al,1994),but little work was donein China.A preliminary molecular map withRFLP,RAPD and SSR was constructed andQTL maping was dong for some agronomic traitsby our research group.
文摘CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used to screen polymorphism among two parents,TM-1 and CSB14Sh,
