The white-rot fungus, Phanerochaete chrysosporium(P. chrysosporium), was inoculated during different phases of agricultural waste composting and its effect on the spectroscopic characterization of humic acid(HA) was s...The white-rot fungus, Phanerochaete chrysosporium(P. chrysosporium), was inoculated during different phases of agricultural waste composting and its effect on the spectroscopic characterization of humic acid(HA) was studied. Three runs were used in this study: Run A was the control without inoculating, and Runs B and C were inoculated P. chrysosporium during the first and the second fermentation phase, respectively. The elemental analysis, ultra-violet spectroscopy(UV), fluorescence spectra, Fourier transform infra-red(FTIR) and 13 C nuclear magnetic resonance(13C-NMR) of HA all lead to the same conclusion, that is, the degree of aromatization and polymerization of HA increases after 42 days composting. However, the inoculation during different phases presents different effects. P. chrysosporium increases the degree of aromatization and polymerization of HA when it is inoculated during the second fermentation phase, while it does not produce an obvious change on the humification degree of HA when it is inoculated during the first fermentation phase.展开更多
The plant pathogen Verticillium dahliae causes severe cotton losses in Uzbekistan. To create cotton varieties that are resistant to the more virulent races of V.dahliae we wanted to determine
In order to obtain an effective and reliable grain refiner for Mg-Al alloys, 1% （mass fraction） Mg3N2 was added into AZ31 Mg alloy. The microstructures of the Mg alloys were studied by optical microscopy, scan elect...In order to obtain an effective and reliable grain refiner for Mg-Al alloys, 1% （mass fraction） Mg3N2 was added into AZ31 Mg alloy. The microstructures of the Mg alloys were studied by optical microscopy, scan electron microscopy and X-ray energy dispersive spectroscopy, and the mechanical properties were determined. The results show that adding a small amount of Mg3N2 to AZ31 Mg alloy can refine the grain size from 103 to 58 μm. The ultimate tensile strength and elongation of AZ31 Mg alloy are 174.1MPa and 8.3%, respectively. After the addition of 1% Mg3N2, the ultimate tensile strength and elongation of AZ31 Mg alloy are increased up to 198.7 MPa and 11.8%, respectively. The grain refinement mechanism is that AIN is formed after Mg3N2 is added. Both AIN and Mg phases are of HCP lattice structure, and the disregistry between Mg phases and AIN along （0001）Mg//（0001）AIN is 3.04%, which is very effective for heterogeneous nucleation.展开更多
The white-rot fungus, Phanerochaete chrysosporium (P. chrysosporium), was inoculated during different phases of agricultural waste composting, and its effect on the fluorescence spectroscopy characteristics of humic a...The white-rot fungus, Phanerochaete chrysosporium (P. chrysosporium), was inoculated during different phases of agricultural waste composting, and its effect on the fluorescence spectroscopy characteristics of humic acid (HA) was studied. The results show that the emission spectra have a sharp peak at 400 nm and a broad shoulder with the maximum centered at 460 nm. The excitation spectra have two peaks and exhibit red shift (shift to longer wavelengths) at 470 nm. The synchronous scan spectra present a number of peaks and shoulders, and the peaks at shorter wavelengths disappear gradually and form a shoulder. At the final stage of composting, the fluorescence spectra have similar shapes, but the fluorescence intensities decrease. P. chrysosporium increases the degree of aromatization and polymerization of HA when it is inoculated during the second fermentation phase, while it does not produce an obvious change on the humification degree of HA when it is inoculated during the first fermentation phase. Compared with the fluorescence spectroscopy characteristics of HA from soil, the structure of HA from compost is simpler and the activity is higher.展开更多
Adenoviruses harboring E. coli. cytosine deaminase(CD) gene (Ad-CD) and murine granulocyte-macrophage colony stimulating factor (GM-CSF) gene(Ad-GM-CSF) were used for gene transfer in vivo.(C57BL/6 mice were inoculate...Adenoviruses harboring E. coli. cytosine deaminase(CD) gene (Ad-CD) and murine granulocyte-macrophage colony stimulating factor (GM-CSF) gene(Ad-GM-CSF) were used for gene transfer in vivo.(C57BL/6 mice were inoculated subeutaneously with FBL-3 erythroleukemia cells and three days later treated withadenovirus injection at the site of tumor inoculation.展开更多
Under field conditions, an experiment was conducted to study the effects of ammonification bacteria, potassium bacteria and phosphorus bacteria on nutrient availability in soil and yield of rice in the cold region of ...Under field conditions, an experiment was conducted to study the effects of ammonification bacteria, potassium bacteria and phosphorus bacteria on nutrient availability in soil and yield of rice in the cold region of China and compared to the conventional fertilization. Results showed that DF1P2 treatment （ammonifiers 1.5× 108 cfu· m2, phosphorus bacteria 1.5× 108 cfu. m2, and potassium bacteria 1.5× 108 cfu· m2） increased available nutrient concentrations in soil, increased the concentrations of N, P, and K in plant organs and increased the rice yield and was the most significantly among all the treatments. This treatment could be recommended as the best suitable biological fertilizer application rate for the rice production in the cold region of China.展开更多
The trail was designed to study on technique aspects of ensiling rice straw （RS） appended amounts of lactobacillus. There were two groups according to silage ways, baled silage （BS） and chopped silage （CS）, in w...The trail was designed to study on technique aspects of ensiling rice straw （RS） appended amounts of lactobacillus. There were two groups according to silage ways, baled silage （BS） and chopped silage （CS）, in which lactobacillus was added at levels of 10, 15 and 20 mg·kg^-1, respectively and the mixtures were placed into a packed polyethylene bags and stored at room temperature for 45 days. The results showed that lactobacillus had remarkable effect on fermentation characteristics of RS. The quality of the silage was improved with the lactobacillus addition. In the experiment the optimal quality of rice straw silage （RSS） can be obtained when lactobacillus was added with 15 or 20 mg·kg^-1 level. The effect of different silage methods was very remarkable to the silage quality of same material. The quality of CS was better than that of long silage, at the same time, BS was feasible on condition of eligible level of lactic acid bacteria.展开更多
Effects of fibroblast-mediated IL-2 and IL-3 genetherapy on recovery of bone marrow depression in tumor-bearing mice treated with cyclophosphamide (300 mg/kg) and syngeneic BMT were investigated in the presentreport. ...Effects of fibroblast-mediated IL-2 and IL-3 genetherapy on recovery of bone marrow depression in tumor-bearing mice treated with cyclophosphamide (300 mg/kg) and syngeneic BMT were investigated in the presentreport. BALB/c mice were inoculated with J558Lplasmacytoma cells s. c. and 3 days later IL-2 secretingNIH3T3-IL-2, IL-3 secreting NIH3T3-IL-3 fibroblastcells were implanted into the tumor-bearing mice展开更多
We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficienc...We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficiency assay showed thatthe infected cancer cells inoculated with theserecombinant adenoviruses at MOI of 25 or greaterexhibited 100% transduction efficiency. The growthrates and colony formation of cancer cells infectedwith these recombinant adenoviruses were展开更多
Antitumor effects of combined transfer of suicidegene and cytokine gene were investigated in this report.Adenovirus harboring E.coli. cytosine deaminase (CD)gene (Ad-CD) and murine interleukin 2 (IL-2) gene(Ad-IL-2) w...Antitumor effects of combined transfer of suicidegene and cytokine gene were investigated in this report.Adenovirus harboring E.coli. cytosine deaminase (CD)gene (Ad-CD) and murine interleukin 2 (IL-2) gene(Ad-IL-2) were used for gene transfer in vitro and invivo. C57BL/6 mice were inoculated subcutaneously withB16F10 melanoma cells and three days later treated withadenovirus injection at the site of tumor inoculation.Significant inhibition of tumor growth was achieved展开更多
The aim of this work was to see whether Pseudomonas putida NWU12, Pseudomonas fluorescence NWU65, Vibrio fluvialis NWU37 and Ewingella americana NWU59 are beneficial to plants and are able to promote plant growth and ...The aim of this work was to see whether Pseudomonas putida NWU12, Pseudomonas fluorescence NWU65, Vibrio fluvialis NWU37 and Ewingella americana NWU59 are beneficial to plants and are able to promote plant growth and development when inoculated as plant growth-promoting rhizobacteria (PGPR). The four rhizobacteria were tested in vitro for PGPR activities and on spinach and pepper in pot experiments. The inoculants are all positive for ammonia (NH3 ), catalase, hydrogen cyanide (HCN), phosphate solubilization and siderophore production. Among the inoculants, E. americana NWU59 is oxidase negative. P. putida NWU12 and P. fluorescence NWU65 are producing indole-3-acetic acid (IAA). The inoculants exhibit some PGPR activities and thus tested in the screen-house. Treatments are control (water) and the four inoculants. Rhizobacterial inoculants increase spinach (17.14%-21.43%) and pepper (15.0%-37.5%) plant heights over the control. Such inoculants have the potential of improving plant yield components and may be used as biofertilizer.展开更多
基金Projects(5110817851108423+2 种基金5103900151378190)supported by the National Natural Science Foundation of ChinaProject(20100161110012)supported by the Research Fund for the Doctoral Program of Higher Education of China
文摘The white-rot fungus, Phanerochaete chrysosporium(P. chrysosporium), was inoculated during different phases of agricultural waste composting and its effect on the spectroscopic characterization of humic acid(HA) was studied. Three runs were used in this study: Run A was the control without inoculating, and Runs B and C were inoculated P. chrysosporium during the first and the second fermentation phase, respectively. The elemental analysis, ultra-violet spectroscopy(UV), fluorescence spectra, Fourier transform infra-red(FTIR) and 13 C nuclear magnetic resonance(13C-NMR) of HA all lead to the same conclusion, that is, the degree of aromatization and polymerization of HA increases after 42 days composting. However, the inoculation during different phases presents different effects. P. chrysosporium increases the degree of aromatization and polymerization of HA when it is inoculated during the second fermentation phase, while it does not produce an obvious change on the humification degree of HA when it is inoculated during the first fermentation phase.
文摘The plant pathogen Verticillium dahliae causes severe cotton losses in Uzbekistan. To create cotton varieties that are resistant to the more virulent races of V.dahliae we wanted to determine
基金Project(2007430023)supported by the Office of Education,Henan Province,ChinaProject(0612002500)supported by the Excellent Young Scientists Foundation of Henan Province,China
文摘In order to obtain an effective and reliable grain refiner for Mg-Al alloys, 1% （mass fraction） Mg3N2 was added into AZ31 Mg alloy. The microstructures of the Mg alloys were studied by optical microscopy, scan electron microscopy and X-ray energy dispersive spectroscopy, and the mechanical properties were determined. The results show that adding a small amount of Mg3N2 to AZ31 Mg alloy can refine the grain size from 103 to 58 μm. The ultimate tensile strength and elongation of AZ31 Mg alloy are 174.1MPa and 8.3%, respectively. After the addition of 1% Mg3N2, the ultimate tensile strength and elongation of AZ31 Mg alloy are increased up to 198.7 MPa and 11.8%, respectively. The grain refinement mechanism is that AIN is formed after Mg3N2 is added. Both AIN and Mg phases are of HCP lattice structure, and the disregistry between Mg phases and AIN along （0001）Mg//（0001）AIN is 3.04%, which is very effective for heterogeneous nucleation.
基金Project(2005CB724203) supported by the Major State Basic Research and Development Program of ChinaProject(IRT0719) supported by the Program for Changjiang Scholars and Innovative Research Team in University of China+1 种基金Projects(50608029,50808073) supported by the National Natural Science Foundation of ChinaProject(2007185) supported by the Environmental Protection Technology Research Program of Hunan Province,China
文摘The white-rot fungus, Phanerochaete chrysosporium (P. chrysosporium), was inoculated during different phases of agricultural waste composting, and its effect on the fluorescence spectroscopy characteristics of humic acid (HA) was studied. The results show that the emission spectra have a sharp peak at 400 nm and a broad shoulder with the maximum centered at 460 nm. The excitation spectra have two peaks and exhibit red shift (shift to longer wavelengths) at 470 nm. The synchronous scan spectra present a number of peaks and shoulders, and the peaks at shorter wavelengths disappear gradually and form a shoulder. At the final stage of composting, the fluorescence spectra have similar shapes, but the fluorescence intensities decrease. P. chrysosporium increases the degree of aromatization and polymerization of HA when it is inoculated during the second fermentation phase, while it does not produce an obvious change on the humification degree of HA when it is inoculated during the first fermentation phase. Compared with the fluorescence spectroscopy characteristics of HA from soil, the structure of HA from compost is simpler and the activity is higher.
文摘Adenoviruses harboring E. coli. cytosine deaminase(CD) gene (Ad-CD) and murine granulocyte-macrophage colony stimulating factor (GM-CSF) gene(Ad-GM-CSF) were used for gene transfer in vivo.(C57BL/6 mice were inoculated subeutaneously with FBL-3 erythroleukemia cells and three days later treated withadenovirus injection at the site of tumor inoculation.
文摘Under field conditions, an experiment was conducted to study the effects of ammonification bacteria, potassium bacteria and phosphorus bacteria on nutrient availability in soil and yield of rice in the cold region of China and compared to the conventional fertilization. Results showed that DF1P2 treatment （ammonifiers 1.5× 108 cfu· m2, phosphorus bacteria 1.5× 108 cfu. m2, and potassium bacteria 1.5× 108 cfu· m2） increased available nutrient concentrations in soil, increased the concentrations of N, P, and K in plant organs and increased the rice yield and was the most significantly among all the treatments. This treatment could be recommended as the best suitable biological fertilizer application rate for the rice production in the cold region of China.
基金Heilongjiang Dairy Project Italian Grant Project
文摘The trail was designed to study on technique aspects of ensiling rice straw （RS） appended amounts of lactobacillus. There were two groups according to silage ways, baled silage （BS） and chopped silage （CS）, in which lactobacillus was added at levels of 10, 15 and 20 mg·kg^-1, respectively and the mixtures were placed into a packed polyethylene bags and stored at room temperature for 45 days. The results showed that lactobacillus had remarkable effect on fermentation characteristics of RS. The quality of the silage was improved with the lactobacillus addition. In the experiment the optimal quality of rice straw silage （RSS） can be obtained when lactobacillus was added with 15 or 20 mg·kg^-1 level. The effect of different silage methods was very remarkable to the silage quality of same material. The quality of CS was better than that of long silage, at the same time, BS was feasible on condition of eligible level of lactic acid bacteria.
文摘Effects of fibroblast-mediated IL-2 and IL-3 genetherapy on recovery of bone marrow depression in tumor-bearing mice treated with cyclophosphamide (300 mg/kg) and syngeneic BMT were investigated in the presentreport. BALB/c mice were inoculated with J558Lplasmacytoma cells s. c. and 3 days later IL-2 secretingNIH3T3-IL-2, IL-3 secreting NIH3T3-IL-3 fibroblastcells were implanted into the tumor-bearing mice
文摘We have constructed three recombinantadenoviruses encoding RA538, p53 or p16 respec-tively. These recombinant adenoviruses are able toexpress RA538, p53 or p16 in infected human cancercells. The transduction efficiency assay showed thatthe infected cancer cells inoculated with theserecombinant adenoviruses at MOI of 25 or greaterexhibited 100% transduction efficiency. The growthrates and colony formation of cancer cells infectedwith these recombinant adenoviruses were
文摘Antitumor effects of combined transfer of suicidegene and cytokine gene were investigated in this report.Adenovirus harboring E.coli. cytosine deaminase (CD)gene (Ad-CD) and murine interleukin 2 (IL-2) gene(Ad-IL-2) were used for gene transfer in vitro and invivo. C57BL/6 mice were inoculated subcutaneously withB16F10 melanoma cells and three days later treated withadenovirus injection at the site of tumor inoculation.Significant inhibition of tumor growth was achieved
基金supported by National Research Foundation of South Africa
文摘The aim of this work was to see whether Pseudomonas putida NWU12, Pseudomonas fluorescence NWU65, Vibrio fluvialis NWU37 and Ewingella americana NWU59 are beneficial to plants and are able to promote plant growth and development when inoculated as plant growth-promoting rhizobacteria (PGPR). The four rhizobacteria were tested in vitro for PGPR activities and on spinach and pepper in pot experiments. The inoculants are all positive for ammonia (NH3 ), catalase, hydrogen cyanide (HCN), phosphate solubilization and siderophore production. Among the inoculants, E. americana NWU59 is oxidase negative. P. putida NWU12 and P. fluorescence NWU65 are producing indole-3-acetic acid (IAA). The inoculants exhibit some PGPR activities and thus tested in the screen-house. Treatments are control (water) and the four inoculants. Rhizobacterial inoculants increase spinach (17.14%-21.43%) and pepper (15.0%-37.5%) plant heights over the control. Such inoculants have the potential of improving plant yield components and may be used as biofertilizer.
