Objective Bone-marrow stem-cell transplantation has been shown to improve cardiac function in patients with AMI, but the safety of intracoronory infusion of autologous peripheral blood stem-cell(PBSCs) in patients wit...Objective Bone-marrow stem-cell transplantation has been shown to improve cardiac function in patients with AMI, but the safety of intracoronory infusion of autologous peripheral blood stem-cell(PBSCs) in patients with AMI is unknown. For this reason, we observe the feasibility and safety of PBSCs transplantation by intracoronory infusion in such patients.Method Fourty one patients with AMI were allocated to receive Granulocyte Colony-Stimulating Factor (G-CSF:Filgrastim,300 μg) with the dose of 300 μg-600 μg/day to mobilize the stem cell, and the duration of applying G-CSF was 5 days . On the sixth day, PBSCs were separated by Baxter CS 3000 blood cell separator into suspend liquid 57 ml. Then the suspend liquid was infused into the infarct related artery (IRA)by occluding the over the wire balloon and infusing artery through balloon center lumen. In the process of the intracoronary infusion of PBSCs, the complications should be observed, which were arrhythmias including of bradycardia, sinus arrest or atrial ventricular block, premature ventricular beats ,ventricular tachycardia, ventricular fibrillation; and hypotention, etc. Results There were total 10 cases with complications during the intracoronary infusion of PBSCs. The incidence of complications was 24.4%(10/41), including bradycardia is 2.4 %(1/41), sinus arrest or atrial ventricular block is 4.9%(2/41), ventricular fibrillation is 2.4 %( 1/41), hypotention is14.6 % (6 /41).Conclusions In patients with AMI, intracoronary infusion of PBSCs is feasible and safe.展开更多
Myocardial infarction(MI)is a disorder that lowers the lifespan and quality of life.Reperfusion treatment as early as possible is the most effective solution,with an increased focus on post-MI medication.In the recove...Myocardial infarction(MI)is a disorder that lowers the lifespan and quality of life.Reperfusion treatment as early as possible is the most effective solution,with an increased focus on post-MI medication.In the recovery process after MI,telocytes(TCs)appear to play an important role,which develops a large number of questions awaiting answers.Defining possible signaling mechanisms involved in recovery after MI may lead to identification the limits of current therapies,and development of new therapeutic solutions.Key words:telocytes;myocardial infarction;stem展开更多
Introduction Leukem ia is a hem atologic neoplasm characterized by potential infectious and hem orrhagic com plications In adult patients with acute leukem ia,in fection is the most com m on com plication.Imtrcra nial...Introduction Leukem ia is a hem atologic neoplasm characterized by potential infectious and hem orrhagic com plications In adult patients with acute leukem ia,in fection is the most com m on com plication.Imtrcra nial hem orrhage (ICH) is the second most common com plication.H ow ever,ICH has been identified as the m ajor cause of m orbidity and m ortaliity in pa tients with leukem ia[1-6].A num ber of case ceports ascribed the death of leukem ic patients due to hem orrhge of the supratentorial and infratentorial brain,basal ganglia,and cerebellum [2,7-9].However,few cases have reported on brainstem hem orrhage.The current case report involved a young wom an who died of brainstem hem orrhage due to acute leukem ia,discussing the pathophysiologic m echanism underly ing ICH.Futhherm ore,the risk factors were specified,w ith a provision of suggestions to forensic pathologists in handling deaths associated w ith ICH,especially sudden unexpected deaths.展开更多
目的分析急性脑梗死(acute cerebral infarction,ACI)患者小而密低密度脂蛋白(small dense low-density lipoprotein,sd-LDL)及脂蛋白(a)水平与颈动脉斑块稳定性的关系。方法回顾性选取2020年2月至2024年2月河北省人民医院收治的老年AC...目的分析急性脑梗死(acute cerebral infarction,ACI)患者小而密低密度脂蛋白(small dense low-density lipoprotein,sd-LDL)及脂蛋白(a)水平与颈动脉斑块稳定性的关系。方法回顾性选取2020年2月至2024年2月河北省人民医院收治的老年ACI患者160例,所有患者行颈部彩色多普勒超声检查,依据颈动脉斑块情况分为无斑块组43例、稳定斑块组56例和不稳定斑块组61例,另取同期河北省人民医院健康体检者40例作为对照组,比较4组临床资料、sd-LDL、脂蛋白(a)水平,评估sd-LDL、脂蛋白(a)水平对不稳定斑块的预测价值。结果与无斑块组比较,稳定斑块组和不稳定斑块组美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)显著升高,不稳定斑块组总胆固醇显著升高,高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)显著降低,对照组NIHSS评分、总胆固醇、三酰甘油、LDL-C显著降低,HDL-C显著升高,差异有统计学意义(P<0.05);与稳定斑块组比较,不稳定斑块组NIHSS评分、LDL-C、sdLDL、脂蛋白(a)显著升高,HDL-C显著降低,对照组脂蛋白(a)显著降低,差异有统计学意义(P<0.05)。Pearson相关性分析显示,sd-LDL、脂蛋白(a)水平与NIHSS评分、总胆固醇、三酰甘油、LDL-C呈显著正相关(P<0.05,P<0.01),与HDL-C呈显著负相关(P<0.01)。二元logistic回归分析显示,NIHSS评分、LDL-C、sd-LDL、脂蛋白(a)是ACI患者颈动脉不稳定斑块形成的危险因素,HDL-C是保护因素(P<0.01)。ROC曲线结果显示,sd-LDL、脂蛋白(a)及联合检测对颈动脉斑块稳定性预测的ROC曲线下面积分别为0.830、0.847、0.921,联合检测的敏感性高于sd-LDL、脂蛋白(a)单项指标检测(93.44%vs 88.52%、86.89%,P=0.000)。结论血浆sd-LDL、脂蛋白(a)水平与ACI患者颈动脉斑块稳定性具有一定关联性,可作为临床相关参考指标。展开更多
目的分析急性脑梗死患者沉默信息调节因子2(silent information regulator 2,SIRT2)与认知功能障碍发生及病情程度的关系。方法回顾性选择2022年5月至2024年5月武汉市第一医院神经内科收治的急性脑梗死合并认知功能障碍患者150例(研究...目的分析急性脑梗死患者沉默信息调节因子2(silent information regulator 2,SIRT2)与认知功能障碍发生及病情程度的关系。方法回顾性选择2022年5月至2024年5月武汉市第一医院神经内科收治的急性脑梗死合并认知功能障碍患者150例(研究组),研究组依据认知功能障碍严重程度分为轻度组45例、中度组66例和重度组39例。另选取同期武汉市第一医院就诊的急性脑梗死未合并认知功能障碍患者125例(对照组)。采用Spearman相关性分析血清SIRT2水平与认知功能障碍及病情程度的相关性,ROC曲线分析血清SIRT2水平对认知功能障碍及病情的评估价值,并计算曲线下面积(area under curve,AUC)。结果研究组血清SIRT2水平明显高于对照组,差异有统计学意义[(20.38±5.19)mg/L vs(14.66±4.49)mg/L,P<0.05]。重度组血清SIRT2水平明显高于中度组和轻度组,中度组血清SIRT2水平明显高于轻度组,差异有统计学意义(P<0.05)。Spearman相关性分析显示,血清SIRT2水平与认知功能障碍发生及严重程度呈正相关(r=0.510,r=0.527,P<0.01)。ROC曲线分析显示,血清SIRT2水平评估急性脑梗死患者认知功能障碍发生的AUC为0.796(95%CI:0.743~0.842),临界值为19.0 mg/L,敏感性为63.33%,特异性为84.00%;血清SIRT2水平评估急性脑梗死患者认知功能障碍严重程度的AUC为0.747(95%CI:0.655~0.824),临界值为17.2 mg/L,敏感性为75.76%,特异性为71.11%(P<0.05)。结论急性脑梗死患者血清SIRT2水平与认知功能障碍发生及病情密切相关。展开更多
文摘Objective Bone-marrow stem-cell transplantation has been shown to improve cardiac function in patients with AMI, but the safety of intracoronory infusion of autologous peripheral blood stem-cell(PBSCs) in patients with AMI is unknown. For this reason, we observe the feasibility and safety of PBSCs transplantation by intracoronory infusion in such patients.Method Fourty one patients with AMI were allocated to receive Granulocyte Colony-Stimulating Factor (G-CSF:Filgrastim,300 μg) with the dose of 300 μg-600 μg/day to mobilize the stem cell, and the duration of applying G-CSF was 5 days . On the sixth day, PBSCs were separated by Baxter CS 3000 blood cell separator into suspend liquid 57 ml. Then the suspend liquid was infused into the infarct related artery (IRA)by occluding the over the wire balloon and infusing artery through balloon center lumen. In the process of the intracoronary infusion of PBSCs, the complications should be observed, which were arrhythmias including of bradycardia, sinus arrest or atrial ventricular block, premature ventricular beats ,ventricular tachycardia, ventricular fibrillation; and hypotention, etc. Results There were total 10 cases with complications during the intracoronary infusion of PBSCs. The incidence of complications was 24.4%(10/41), including bradycardia is 2.4 %(1/41), sinus arrest or atrial ventricular block is 4.9%(2/41), ventricular fibrillation is 2.4 %( 1/41), hypotention is14.6 % (6 /41).Conclusions In patients with AMI, intracoronary infusion of PBSCs is feasible and safe.
基金Project supported by the Romanian National Authority for Scientific Research,CNCS-UEFISCDI,PNII(82/2012,194/2014)
文摘Myocardial infarction(MI)is a disorder that lowers the lifespan and quality of life.Reperfusion treatment as early as possible is the most effective solution,with an increased focus on post-MI medication.In the recovery process after MI,telocytes(TCs)appear to play an important role,which develops a large number of questions awaiting answers.Defining possible signaling mechanisms involved in recovery after MI may lead to identification the limits of current therapies,and development of new therapeutic solutions.Key words:telocytes;myocardial infarction;stem
基金This study was funded by Natural Science Foundation of China
文摘Introduction Leukem ia is a hem atologic neoplasm characterized by potential infectious and hem orrhagic com plications In adult patients with acute leukem ia,in fection is the most com m on com plication.Imtrcra nial hem orrhage (ICH) is the second most common com plication.H ow ever,ICH has been identified as the m ajor cause of m orbidity and m ortaliity in pa tients with leukem ia[1-6].A num ber of case ceports ascribed the death of leukem ic patients due to hem orrhge of the supratentorial and infratentorial brain,basal ganglia,and cerebellum [2,7-9].However,few cases have reported on brainstem hem orrhage.The current case report involved a young wom an who died of brainstem hem orrhage due to acute leukem ia,discussing the pathophysiologic m echanism underly ing ICH.Futhherm ore,the risk factors were specified,w ith a provision of suggestions to forensic pathologists in handling deaths associated w ith ICH,especially sudden unexpected deaths.
文摘目的分析急性脑梗死(acute cerebral infarction,ACI)患者小而密低密度脂蛋白(small dense low-density lipoprotein,sd-LDL)及脂蛋白(a)水平与颈动脉斑块稳定性的关系。方法回顾性选取2020年2月至2024年2月河北省人民医院收治的老年ACI患者160例,所有患者行颈部彩色多普勒超声检查,依据颈动脉斑块情况分为无斑块组43例、稳定斑块组56例和不稳定斑块组61例,另取同期河北省人民医院健康体检者40例作为对照组,比较4组临床资料、sd-LDL、脂蛋白(a)水平,评估sd-LDL、脂蛋白(a)水平对不稳定斑块的预测价值。结果与无斑块组比较,稳定斑块组和不稳定斑块组美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)显著升高,不稳定斑块组总胆固醇显著升高,高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)显著降低,对照组NIHSS评分、总胆固醇、三酰甘油、LDL-C显著降低,HDL-C显著升高,差异有统计学意义(P<0.05);与稳定斑块组比较,不稳定斑块组NIHSS评分、LDL-C、sdLDL、脂蛋白(a)显著升高,HDL-C显著降低,对照组脂蛋白(a)显著降低,差异有统计学意义(P<0.05)。Pearson相关性分析显示,sd-LDL、脂蛋白(a)水平与NIHSS评分、总胆固醇、三酰甘油、LDL-C呈显著正相关(P<0.05,P<0.01),与HDL-C呈显著负相关(P<0.01)。二元logistic回归分析显示,NIHSS评分、LDL-C、sd-LDL、脂蛋白(a)是ACI患者颈动脉不稳定斑块形成的危险因素,HDL-C是保护因素(P<0.01)。ROC曲线结果显示,sd-LDL、脂蛋白(a)及联合检测对颈动脉斑块稳定性预测的ROC曲线下面积分别为0.830、0.847、0.921,联合检测的敏感性高于sd-LDL、脂蛋白(a)单项指标检测(93.44%vs 88.52%、86.89%,P=0.000)。结论血浆sd-LDL、脂蛋白(a)水平与ACI患者颈动脉斑块稳定性具有一定关联性,可作为临床相关参考指标。
文摘目的分析急性脑梗死患者沉默信息调节因子2(silent information regulator 2,SIRT2)与认知功能障碍发生及病情程度的关系。方法回顾性选择2022年5月至2024年5月武汉市第一医院神经内科收治的急性脑梗死合并认知功能障碍患者150例(研究组),研究组依据认知功能障碍严重程度分为轻度组45例、中度组66例和重度组39例。另选取同期武汉市第一医院就诊的急性脑梗死未合并认知功能障碍患者125例(对照组)。采用Spearman相关性分析血清SIRT2水平与认知功能障碍及病情程度的相关性,ROC曲线分析血清SIRT2水平对认知功能障碍及病情的评估价值,并计算曲线下面积(area under curve,AUC)。结果研究组血清SIRT2水平明显高于对照组,差异有统计学意义[(20.38±5.19)mg/L vs(14.66±4.49)mg/L,P<0.05]。重度组血清SIRT2水平明显高于中度组和轻度组,中度组血清SIRT2水平明显高于轻度组,差异有统计学意义(P<0.05)。Spearman相关性分析显示,血清SIRT2水平与认知功能障碍发生及严重程度呈正相关(r=0.510,r=0.527,P<0.01)。ROC曲线分析显示,血清SIRT2水平评估急性脑梗死患者认知功能障碍发生的AUC为0.796(95%CI:0.743~0.842),临界值为19.0 mg/L,敏感性为63.33%,特异性为84.00%;血清SIRT2水平评估急性脑梗死患者认知功能障碍严重程度的AUC为0.747(95%CI:0.655~0.824),临界值为17.2 mg/L,敏感性为75.76%,特异性为71.11%(P<0.05)。结论急性脑梗死患者血清SIRT2水平与认知功能障碍发生及病情密切相关。
文摘目的探讨急性脑梗死(acute cerebral infarction,ACI)患者脑电图、血清胰岛素生长因子1(insulin-like growth factor 1,IGF-1)、神经元特异性烯醇化酶(neuron-specific enolase,NSE)与病灶体积及美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评分的关系。方法选择2021年8月至2022年12月在南京市溧水区人民医院神经内科首次确诊的ACI患者218例,根据病灶体积分为大体积组63例、中体积组103例和小体积组52例。检测患者脑电图(δ+θ)与(α+β)功率比[(δ+θ)/(α+β)ratio,DTABR]、大脑对称指数(brainspine interface,BSI)、血清IGF-1和NSE水平,观察上述指标与MRI检查脑梗死病灶体积、NIHSS评分、阿替普酶溶栓后2周、4周时NIHSS评分的相关性。结果中体积组和大体积组IGF-1水平明显低于小体积组,NSE、DTABR、BSI明显高于小体积组(P<0.05);大体积组IGF-1水平明显低于中体积组,NSE、DTABR、BSI明显高于中体积组(P<0.05)。DTABR、BSI、血清NSE与病灶体积(r=0.563,P=0.000;r=0.318,P=0.038;r=0.673,P=0.000)和治疗前NIHSS评分(r=0.499,P=0.000;r=0.362,P=0.013;r=0.750,P=0.001)呈显著正相关。血清IGF-1水平与病灶体积(r=-0.572,P=0.000)和治疗前NIHSS评分(r=-0.438,P=0.001)呈显著负相关。DTABR、BSI、血清NSE、病灶体积均与溶栓后2、4周NIHSS评分呈正相关,IGF-1与溶栓后2、4周NIHSS评分呈负相关(P<0.05,P<0.01)。结论ACI患者脑电图、IGF-1和NSE与病灶体积和溶栓后NIHSS评分显著相关。
