The rDNA internal transcribed spacer 1(ITS-1) regions of two Chinese fir provanences was amplified and cloned by PCR reaction.The PCR reaction was following:97℃ 5 minutes→95℃ 5 minhtes→Adding the Tag polymerase→9...The rDNA internal transcribed spacer 1(ITS-1) regions of two Chinese fir provanences was amplified and cloned by PCR reaction.The PCR reaction was following:97℃ 5 minutes→95℃ 5 minhtes→Adding the Tag polymerase→94℃ l?min 56℃ 1?min,72℃ 2?min;thirty\|six cycles→72 ℃ 10?min.High quality DNA template is necessary for the amplification of ITS-1 sequence,during the PCR reaction,ten minutes denaturation time and 56℃ annealing temperature are beneficial to amplification.The ITS-1 fragment was ligated to PUC19 plasmid,digested with Hind Ⅱ and transformed into competence cells of E.coli JM83 strain,the cloned strains harboring recombinant plasmid were obtained,those recombinant plasmids were used to sequence for ITS-1 fragment.Sequence analysis indicated that the sequence length is 273 bp,the using percentage of A\,T\,C\,G within ITS1 sequence of Chinese fir were 27.5%\,23%\,21.6%\,27.9% respectively and the G/C content of ITS1 sequence was 48.35%.Comparing with other plants,the G/C content of Chinese fir was less than other plants,whose ITS1 regions have been sequenced.As to ITSI sequence,there was no difference among two Chinese fir provenances,sequence analysis disclosed there were two repeat sequences [AAAG] n and [TTG] nappeared within ITS1 sequence of Chinese fir.展开更多
In [3], they gave necessary and sufficient condition for T 1 C and then as applications T 1 C for weakly dependent sequences was established. In this note, based on Gozlan-L′eonard characterization for W 1 H -inequal...In [3], they gave necessary and sufficient condition for T 1 C and then as applications T 1 C for weakly dependent sequences was established. In this note, based on Gozlan-L′eonard characterization for W 1 H -inequalities, we extends this result to W 1 H inequalities.展开更多
Both cDNA and DNA clones of PtDof1 （GenBank Accession No. FJ402844 and FJ402845） were isolated from plants grown in tissue culture ofPopulus tornentosa. The DNA sequence is 1597 bp including two exons and one intron...Both cDNA and DNA clones of PtDof1 （GenBank Accession No. FJ402844 and FJ402845） were isolated from plants grown in tissue culture ofPopulus tornentosa. The DNA sequence is 1597 bp including two exons and one intron. The cDNA is 969 bp in length with a 765 bp open reading frame which is capable of encoding 255 amino acids. The deduced amino acids sequence of the PtDofl protein shares 65%, 56% and 55% identity with Vitis vinifera （CAO48618）, Nicotiana tabacum （CAA08755） and Glycine max （ABI 16022） Dof protein by blast analysis in GenBank. Phylogenic analysis suggests PtDof1 gene could belong to the Dofgene family. PtDofl protein contains an unusual conserved single zinc finger with the pattern of C-X2-C-X21-C-X2-C, which may play a functional role in tissue-specific expression and possibly the auxin response of endogenous plant genes.展开更多
Objective: To know some genetical characterizations of Coxiella burnetii Chinese isolates by comparing the coml gene sequence. Methods: com1 gene sequences of Chinese isolates were amplified, se-quenced, and analyzed ...Objective: To know some genetical characterizations of Coxiella burnetii Chinese isolates by comparing the coml gene sequence. Methods: com1 gene sequences of Chinese isolates were amplified, se-quenced, and analyzed by comparing our result and the previous published data. Results: Three different com1 sequences were identified in 7 Chinese isolates. Sequence comparison indicated that the isolates harboring the QpRS plasmid could be defined as a new group and, in addition, the isolates carrying the same plas-mid type showed similar com1 gene sequence. Conclusion: Study suggests that the classification of the group based on the coml gene sequence is highly associated with the plasmid type of the isolates and, however, little related to disease forms and geographical origins of the isolates.展开更多
文摘The rDNA internal transcribed spacer 1(ITS-1) regions of two Chinese fir provanences was amplified and cloned by PCR reaction.The PCR reaction was following:97℃ 5 minutes→95℃ 5 minhtes→Adding the Tag polymerase→94℃ l?min 56℃ 1?min,72℃ 2?min;thirty\|six cycles→72 ℃ 10?min.High quality DNA template is necessary for the amplification of ITS-1 sequence,during the PCR reaction,ten minutes denaturation time and 56℃ annealing temperature are beneficial to amplification.The ITS-1 fragment was ligated to PUC19 plasmid,digested with Hind Ⅱ and transformed into competence cells of E.coli JM83 strain,the cloned strains harboring recombinant plasmid were obtained,those recombinant plasmids were used to sequence for ITS-1 fragment.Sequence analysis indicated that the sequence length is 273 bp,the using percentage of A\,T\,C\,G within ITS1 sequence of Chinese fir were 27.5%\,23%\,21.6%\,27.9% respectively and the G/C content of ITS1 sequence was 48.35%.Comparing with other plants,the G/C content of Chinese fir was less than other plants,whose ITS1 regions have been sequenced.As to ITSI sequence,there was no difference among two Chinese fir provenances,sequence analysis disclosed there were two repeat sequences [AAAG] n and [TTG] nappeared within ITS1 sequence of Chinese fir.
文摘In [3], they gave necessary and sufficient condition for T 1 C and then as applications T 1 C for weakly dependent sequences was established. In this note, based on Gozlan-L′eonard characterization for W 1 H -inequalities, we extends this result to W 1 H inequalities.
基金supported by the National Natural Science Foundation of China (Grant No. 30271097)
文摘Both cDNA and DNA clones of PtDof1 （GenBank Accession No. FJ402844 and FJ402845） were isolated from plants grown in tissue culture ofPopulus tornentosa. The DNA sequence is 1597 bp including two exons and one intron. The cDNA is 969 bp in length with a 765 bp open reading frame which is capable of encoding 255 amino acids. The deduced amino acids sequence of the PtDofl protein shares 65%, 56% and 55% identity with Vitis vinifera （CAO48618）, Nicotiana tabacum （CAA08755） and Glycine max （ABI 16022） Dof protein by blast analysis in GenBank. Phylogenic analysis suggests PtDof1 gene could belong to the Dofgene family. PtDofl protein contains an unusual conserved single zinc finger with the pattern of C-X2-C-X21-C-X2-C, which may play a functional role in tissue-specific expression and possibly the auxin response of endogenous plant genes.
基金Supported by Science Research Grant from the Ministry of E-ducation, Science, Sports and Culture of Japan (No. 10460140 and 11556060), and by Health Sciences Research Grant on Emerging and Re-emerging Infectious Diseases from the Ministry of Health and W
文摘Objective: To know some genetical characterizations of Coxiella burnetii Chinese isolates by comparing the coml gene sequence. Methods: com1 gene sequences of Chinese isolates were amplified, se-quenced, and analyzed by comparing our result and the previous published data. Results: Three different com1 sequences were identified in 7 Chinese isolates. Sequence comparison indicated that the isolates harboring the QpRS plasmid could be defined as a new group and, in addition, the isolates carrying the same plas-mid type showed similar com1 gene sequence. Conclusion: Study suggests that the classification of the group based on the coml gene sequence is highly associated with the plasmid type of the isolates and, however, little related to disease forms and geographical origins of the isolates.
