Objective:To confirm that double-axis rotation activates vestibular nuclear complex(VNC)via stimulating vestibular endorgans.Methods:Sixteen male Sprague-Dawley rats were randomly divided equally into four groups:cont...Objective:To confirm that double-axis rotation activates vestibular nuclear complex(VNC)via stimulating vestibular endorgans.Methods:Sixteen male Sprague-Dawley rats were randomly divided equally into four groups:control,rotation,vestibular-lesioned and vestibular-lesioned plus roatation.Lesion of vestibular endorgans were accomplished through tanstympanic injection of 100 mg of sodium arsanilate.All animals were singly restrained in custommade Perspex containers and placed in darkness.Rats in rotation and vestibular-lesioned plus rotation groups were subjected to double-axis rotation for 2 h,while others as control were positioned close to the stimulator.Following stimulation,all rats were anesthetically killed and the brain tissues removed to examine Fos expression level using Western Blot.Results:Compared with normal control,rats in rotation group exhibited a tendency to increase in Fos expression in the VNC,although without statistical significance(P>0.05).The Fos expression levels in vestibular-lesioned and vestibular-lesioned plus rotation groups approximated.When normalized to the Fos expression in normal control group,the values representing Fos level in rotation,vestibular-lesioned and vestibular-leioned plus rotation groups were 1.129±0.0631,0.959±0.0487 and 1.023±0.237,respectively.Conclusion:Double-axis rotation can activate VNC neurons,and sodium arsanilate can functionally disrupt vestibular endorgans.展开更多
文摘Objective:To confirm that double-axis rotation activates vestibular nuclear complex(VNC)via stimulating vestibular endorgans.Methods:Sixteen male Sprague-Dawley rats were randomly divided equally into four groups:control,rotation,vestibular-lesioned and vestibular-lesioned plus roatation.Lesion of vestibular endorgans were accomplished through tanstympanic injection of 100 mg of sodium arsanilate.All animals were singly restrained in custommade Perspex containers and placed in darkness.Rats in rotation and vestibular-lesioned plus rotation groups were subjected to double-axis rotation for 2 h,while others as control were positioned close to the stimulator.Following stimulation,all rats were anesthetically killed and the brain tissues removed to examine Fos expression level using Western Blot.Results:Compared with normal control,rats in rotation group exhibited a tendency to increase in Fos expression in the VNC,although without statistical significance(P>0.05).The Fos expression levels in vestibular-lesioned and vestibular-lesioned plus rotation groups approximated.When normalized to the Fos expression in normal control group,the values representing Fos level in rotation,vestibular-lesioned and vestibular-leioned plus rotation groups were 1.129±0.0631,0.959±0.0487 and 1.023±0.237,respectively.Conclusion:Double-axis rotation can activate VNC neurons,and sodium arsanilate can functionally disrupt vestibular endorgans.
