Background Electroacupuncture pretreatment plays a protective role in myocardial ischemia/reperfusion （I/R） injury and microRNAs （miRNAs） could act on various facets of cardiac function. However, the role of miRNA...Background Electroacupuncture pretreatment plays a protective role in myocardial ischemia/reperfusion （I/R） injury and microRNAs （miRNAs） could act on various facets of cardiac function. However, the role of miRNAs in the cardioprotection by electroacupuncture pre-treatment on myocardial I/R injury remains unknown. The purpose of the study was to examine whether miR-214 was involved in cardio-protection by electroacupuncture. Methods Using rat myocardial I/R model, we examined the role of electroacupuncture pretreatment in myocardial I/R injury and analyzed the changes in the expression of miR-214. In addition, I/R was simulated in vitro by performing oxy-gen-glucose deprivation （OGD） on H9c2 cell cultures, and the effect of electroacupuncture pretreatment on I/R injury as well as expressional level of miR-214 were examined in vitro. Furthermore, the miR-214 mimic was transfected into OGD-treated H9c2 cells, we analyzed the cell apoptosis, lactate dehydrogenase （LDH） and creatine kinase （CK） activities, intracellular free Ca2＋concentration （[Ca2＋]i） as well as the relative protein levels of sodium/calcium exchanger 1（NCX1）, BCL2-like 11 （BIM）, calmodulin-dependent protein kinase IIδ（CaMKIIδ） and Cyclophilin D （CypD）. Results The in vivo results revealed that compared with the I/R group, the electroacupuncture pretreatment group showed significant decreased myocardial infarct size, as well as the increased indices of the cardiac function, including heart rate, mean arterial pressure, left ventricular systolic pressure and maximal rate for left ventricular pressure rising and declining （±dp/dt max）. In addition, electroacupuncture pretreatment could inhibit the elevation of LDH and CK activities induced by I/R injury. The quantitative PCR （qPCR） results demonstrated electroacupuncture pretreatment could provide cardioprotection against myocardial I/R injury in rats with miR-214 up-regulation. In the meanwhile, in vitro, electroacupuncture pretreatment protected H9c2 cells from OGD-induced injury. Trans-fection of miR-214 mimic showed protective effects on OGD-induced injury to H9c2 cells by reducing apoptosis, decreasing LDH and CK activities, rescuing the OGD-induced Ca2＋and down-regulating elevated protein levels of NCX1, BIM, CaMKIIδand CypD. Conclusions Our findings firstly demonstrated that electroacupuncture pretreatment promotes the expression of miR-214 in myocardial I/R injury and miR-214 contributes to the protective effect of electroacupuncture on myocardial I/R injury.展开更多
Immunoreactive substance P(Ir-SP)level,and substance P likeimmunoreactivity(SP-Li)in the spinal cord were observed with radioimmunoassay andimmunohistochemistry in rats after they were given an intraperitoneal injecti...Immunoreactive substance P(Ir-SP)level,and substance P likeimmunoreactivity(SP-Li)in the spinal cord were observed with radioimmunoassay andimmunohistochemistry in rats after they were given an intraperitoneal injection of mor-phine(7.5mg/kg)or electroacupunctured(3V and 3Hz)on the “Jiaji point”.It wasfound that the pain threshold(PT),Ir-SP level and SP-Li in the dorsal horn of the spi-nal cord were more significantly increased in the animals after the administration ofmorphine or electroacupuncture than in the control(P【0.05～0.01).The combined effectsof morphine and electropacupuncture were even more powerful than either of the agentswas administered singly.Naloxone could block the analgesic effect and the elevation ofIr-SP due to morphine or electroacupuncture.The findings suggest that there is a synergismbe tween morphine and electroacupuncture and the analgesic effect of the 2 depends uponthe increase of Ir-SP level of the spinal cord mediated through the opiate receptors.展开更多
Fifty SD rats were randomly divided into control,sham,model and electroacupuncture treatment group in this study.AD model rats were established by injecting ABas.ss into the hippocampi,while the same amount of saline ...Fifty SD rats were randomly divided into control,sham,model and electroacupuncture treatment group in this study.AD model rats were established by injecting ABas.ss into the hippocampi,while the same amount of saline was injected into the sham rats.AD model rats were treated by electroacupuncture stimulation at"Baihui"and"Shenshu"acupoints once a day for 10 days.The behavioral experiments results showed that the learning and memory ability of the AD rats were significantly decreased compared with the controls and shams,while depression-related behaviors were significantly increased.展开更多
目的评价电针治疗非急性期周围性面瘫的临床疗效,为临床实践提供科学的循证医学证据。方法检索至2023年7月7日中国知网、万方数据库、维普中文生物医学期刊数据库、中国生物医学文献数据库、PubMed、Cochrane图书馆、Web of Science7个...目的评价电针治疗非急性期周围性面瘫的临床疗效,为临床实践提供科学的循证医学证据。方法检索至2023年7月7日中国知网、万方数据库、维普中文生物医学期刊数据库、中国生物医学文献数据库、PubMed、Cochrane图书馆、Web of Science7个公开数据库中关于电针治疗非急性期周围性面瘫的临床随机对照试验。运用Note Express软件筛选符合标准文献,运用Cochrane系统对纳入文献进行资料提取及质量评价,将以H-B为评判指标的总有效率、治愈率,sunnybrook评分,Portmann评分及面瘫评分等为结局指标,采用Review Manage5.3软件进行异质性检验及Meta分析。结果共检索出文献745篇,最终纳入8篇,共计患者452例。Meta分析显示:电针组对于非急性期周围性面瘫的总有效率(RR=1.23,95%CI:1.12~1.34,P<0.00001)和治愈率(RR=2.14,95%CI:1.61~2.85,P<0.00001),在改善非急性期周围性面瘫患者sunnybrook静态下评分(SMD=0.73,95%CI:0.39~1.08,P<0.0001)、随意肌运动下评分(SMD=0.55,95%CI:0.20~0.89,P=0.002),Portmann评分及面瘫评分(SMD=2.58,95%CI:1.62~3.53,P<0.00001)等方面均优于对照组疗法。结论电针治疗非急性期周面瘫的疗效较其他疗法有一定优越性。但由于本研究纳入文献的样本量有限、对照组疗法种类较少、方法学质量不高,可能产生偏倚,故需要更多高质量临床随机对照试验来验证电针治疗非急性期周围性面瘫面瘫的临床疗效。展开更多
目的探讨电针廉泉穴对脑卒中后吞咽困难(PSD)大鼠神经功能缺损的影响及潜在对瞬时受体电位香草酸亚型1(TRPV1)信号通路的调节机制作用。方法选用SPF级SD雄性大鼠60只,随机分为正常组12只(仅浅插栓线,未导致脑内动脉闭塞),余48只制作PSD...目的探讨电针廉泉穴对脑卒中后吞咽困难(PSD)大鼠神经功能缺损的影响及潜在对瞬时受体电位香草酸亚型1(TRPV1)信号通路的调节机制作用。方法选用SPF级SD雄性大鼠60只,随机分为正常组12只(仅浅插栓线,未导致脑内动脉闭塞),余48只制作PSD模型,将造模成功的36只大鼠随机分为模型组、治疗组和治疗+咖啡酸组,每组12只。记录大鼠吞咽潜伏期和吞咽次数,生物信号采集器检测舌下神经放电、舌肌阈强度和收缩幅度,酶联免疫吸附测定血清P物质含量,甲苯胺蓝染色检测舌下神经核尼氏体数目,免疫组织化学检测舌下神经核TRPV1、五羟色胺(5-HT)、磷酸化p38、神经元型一氧化氮合酶(nNOS)蛋白表达水平。结果与正常组比较,模型组大鼠吞咽潜伏期、吞咽次数、舌下神经放电积分面积、舌肌收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平下降,舌肌阈强度和舌下神经核磷酸化p38、nNOS蛋白表达水平增加(P<0.05);与模型组比较,治疗组大鼠舌肌单收缩幅度、舌肌强直收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平增加[2.36±0.26 vs 1.77±0.22、3.46±0.36 vs 2.15±0.18、(3.92±0.38)ng/ml vs(1.69±0.17)ng/ml、(33.60±3.65)个vs(24.60±2.34)个、(19.85±2.11)%vs(9.79±1.07)%、(22.43±2.34)%vs(10.85±1.13)%,P<0.05]。结论电针廉泉穴可能通过激活TRPV1信号通路改善PSD大鼠神经功能缺损。展开更多
基金This study was supported by a grant from the Hainan Provincial Nature Science Foundation
文摘Background Electroacupuncture pretreatment plays a protective role in myocardial ischemia/reperfusion （I/R） injury and microRNAs （miRNAs） could act on various facets of cardiac function. However, the role of miRNAs in the cardioprotection by electroacupuncture pre-treatment on myocardial I/R injury remains unknown. The purpose of the study was to examine whether miR-214 was involved in cardio-protection by electroacupuncture. Methods Using rat myocardial I/R model, we examined the role of electroacupuncture pretreatment in myocardial I/R injury and analyzed the changes in the expression of miR-214. In addition, I/R was simulated in vitro by performing oxy-gen-glucose deprivation （OGD） on H9c2 cell cultures, and the effect of electroacupuncture pretreatment on I/R injury as well as expressional level of miR-214 were examined in vitro. Furthermore, the miR-214 mimic was transfected into OGD-treated H9c2 cells, we analyzed the cell apoptosis, lactate dehydrogenase （LDH） and creatine kinase （CK） activities, intracellular free Ca2＋concentration （[Ca2＋]i） as well as the relative protein levels of sodium/calcium exchanger 1（NCX1）, BCL2-like 11 （BIM）, calmodulin-dependent protein kinase IIδ（CaMKIIδ） and Cyclophilin D （CypD）. Results The in vivo results revealed that compared with the I/R group, the electroacupuncture pretreatment group showed significant decreased myocardial infarct size, as well as the increased indices of the cardiac function, including heart rate, mean arterial pressure, left ventricular systolic pressure and maximal rate for left ventricular pressure rising and declining （±dp/dt max）. In addition, electroacupuncture pretreatment could inhibit the elevation of LDH and CK activities induced by I/R injury. The quantitative PCR （qPCR） results demonstrated electroacupuncture pretreatment could provide cardioprotection against myocardial I/R injury in rats with miR-214 up-regulation. In the meanwhile, in vitro, electroacupuncture pretreatment protected H9c2 cells from OGD-induced injury. Trans-fection of miR-214 mimic showed protective effects on OGD-induced injury to H9c2 cells by reducing apoptosis, decreasing LDH and CK activities, rescuing the OGD-induced Ca2＋and down-regulating elevated protein levels of NCX1, BIM, CaMKIIδand CypD. Conclusions Our findings firstly demonstrated that electroacupuncture pretreatment promotes the expression of miR-214 in myocardial I/R injury and miR-214 contributes to the protective effect of electroacupuncture on myocardial I/R injury.
文摘Immunoreactive substance P(Ir-SP)level,and substance P likeimmunoreactivity(SP-Li)in the spinal cord were observed with radioimmunoassay andimmunohistochemistry in rats after they were given an intraperitoneal injection of mor-phine(7.5mg/kg)or electroacupunctured(3V and 3Hz)on the “Jiaji point”.It wasfound that the pain threshold(PT),Ir-SP level and SP-Li in the dorsal horn of the spi-nal cord were more significantly increased in the animals after the administration ofmorphine or electroacupuncture than in the control(P【0.05～0.01).The combined effectsof morphine and electropacupuncture were even more powerful than either of the agentswas administered singly.Naloxone could block the analgesic effect and the elevation ofIr-SP due to morphine or electroacupuncture.The findings suggest that there is a synergismbe tween morphine and electroacupuncture and the analgesic effect of the 2 depends uponthe increase of Ir-SP level of the spinal cord mediated through the opiate receptors.
文摘Fifty SD rats were randomly divided into control,sham,model and electroacupuncture treatment group in this study.AD model rats were established by injecting ABas.ss into the hippocampi,while the same amount of saline was injected into the sham rats.AD model rats were treated by electroacupuncture stimulation at"Baihui"and"Shenshu"acupoints once a day for 10 days.The behavioral experiments results showed that the learning and memory ability of the AD rats were significantly decreased compared with the controls and shams,while depression-related behaviors were significantly increased.
文摘目的评价电针治疗非急性期周围性面瘫的临床疗效,为临床实践提供科学的循证医学证据。方法检索至2023年7月7日中国知网、万方数据库、维普中文生物医学期刊数据库、中国生物医学文献数据库、PubMed、Cochrane图书馆、Web of Science7个公开数据库中关于电针治疗非急性期周围性面瘫的临床随机对照试验。运用Note Express软件筛选符合标准文献,运用Cochrane系统对纳入文献进行资料提取及质量评价,将以H-B为评判指标的总有效率、治愈率,sunnybrook评分,Portmann评分及面瘫评分等为结局指标,采用Review Manage5.3软件进行异质性检验及Meta分析。结果共检索出文献745篇,最终纳入8篇,共计患者452例。Meta分析显示:电针组对于非急性期周围性面瘫的总有效率(RR=1.23,95%CI:1.12~1.34,P<0.00001)和治愈率(RR=2.14,95%CI:1.61~2.85,P<0.00001),在改善非急性期周围性面瘫患者sunnybrook静态下评分(SMD=0.73,95%CI:0.39~1.08,P<0.0001)、随意肌运动下评分(SMD=0.55,95%CI:0.20~0.89,P=0.002),Portmann评分及面瘫评分(SMD=2.58,95%CI:1.62~3.53,P<0.00001)等方面均优于对照组疗法。结论电针治疗非急性期周面瘫的疗效较其他疗法有一定优越性。但由于本研究纳入文献的样本量有限、对照组疗法种类较少、方法学质量不高,可能产生偏倚,故需要更多高质量临床随机对照试验来验证电针治疗非急性期周围性面瘫面瘫的临床疗效。
文摘目的探讨电针廉泉穴对脑卒中后吞咽困难(PSD)大鼠神经功能缺损的影响及潜在对瞬时受体电位香草酸亚型1(TRPV1)信号通路的调节机制作用。方法选用SPF级SD雄性大鼠60只,随机分为正常组12只(仅浅插栓线,未导致脑内动脉闭塞),余48只制作PSD模型,将造模成功的36只大鼠随机分为模型组、治疗组和治疗+咖啡酸组,每组12只。记录大鼠吞咽潜伏期和吞咽次数,生物信号采集器检测舌下神经放电、舌肌阈强度和收缩幅度,酶联免疫吸附测定血清P物质含量,甲苯胺蓝染色检测舌下神经核尼氏体数目,免疫组织化学检测舌下神经核TRPV1、五羟色胺(5-HT)、磷酸化p38、神经元型一氧化氮合酶(nNOS)蛋白表达水平。结果与正常组比较,模型组大鼠吞咽潜伏期、吞咽次数、舌下神经放电积分面积、舌肌收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平下降,舌肌阈强度和舌下神经核磷酸化p38、nNOS蛋白表达水平增加(P<0.05);与模型组比较,治疗组大鼠舌肌单收缩幅度、舌肌强直收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平增加[2.36±0.26 vs 1.77±0.22、3.46±0.36 vs 2.15±0.18、(3.92±0.38)ng/ml vs(1.69±0.17)ng/ml、(33.60±3.65)个vs(24.60±2.34)个、(19.85±2.11)%vs(9.79±1.07)%、(22.43±2.34)%vs(10.85±1.13)%,P<0.05]。结论电针廉泉穴可能通过激活TRPV1信号通路改善PSD大鼠神经功能缺损。
