A method for simultaneous determination of oxytetracycline,tetracycline,chlortetracycline in animal tissue was successfully established by HPLC-ESI MS/MS.The tetracycline antibiotics in animal tissue were extracted by...A method for simultaneous determination of oxytetracycline,tetracycline,chlortetracycline in animal tissue was successfully established by HPLC-ESI MS/MS.The tetracycline antibiotics in animal tissue were extracted by liquid-solid extraction and cleaned up by SPE.The mass spectrometer was operated in the positive-ion mode using selected reaction monitoring(SRM).The results showed that three tetracycline antibiotics were well separated under the optimal conditions.The detection limits of oxytetracycline,tetracycline and chlortetracycline were 0.16 ng/g,0.1 ng/g and 0.1 ng/g,respectively.The spiked recoveries were range of 84%-94% and the relative standard deviations were 2.4%-2.9%.展开更多
文摘A method for simultaneous determination of oxytetracycline,tetracycline,chlortetracycline in animal tissue was successfully established by HPLC-ESI MS/MS.The tetracycline antibiotics in animal tissue were extracted by liquid-solid extraction and cleaned up by SPE.The mass spectrometer was operated in the positive-ion mode using selected reaction monitoring(SRM).The results showed that three tetracycline antibiotics were well separated under the optimal conditions.The detection limits of oxytetracycline,tetracycline and chlortetracycline were 0.16 ng/g,0.1 ng/g and 0.1 ng/g,respectively.The spiked recoveries were range of 84%-94% and the relative standard deviations were 2.4%-2.9%.
