为利用斑马鱼动物模型进一步研究bHLH转录因子家庭重要成员hand2基因在心脏发育中的功能,采用生物信息学结合RT-PCR的方法获得了斑马鱼hand2基因.将所得片段插入原核表达载体pGEX-4T-1中,并通过IPTG诱导表达出GST-Hand2融合蛋白,经GST...为利用斑马鱼动物模型进一步研究bHLH转录因子家庭重要成员hand2基因在心脏发育中的功能,采用生物信息学结合RT-PCR的方法获得了斑马鱼hand2基因.将所得片段插入原核表达载体pGEX-4T-1中,并通过IPTG诱导表达出GST-Hand2融合蛋白,经GST亲和层析法纯化后,免疫新西兰大白兔制备了多克隆抗体,利用western b lotting和免疫组化的方法对抗体进行分析.分析表明:斑马鱼hand2基因成熟肽编码区含有627 bp,编码208个氨基酸,与人Hand2蛋白的同源性达到83%;IPTG诱导表达后,表达的融合蛋白占菌体总蛋白的71%,经GST纯化获得SDS-PAGE电泳下单一条带.融合蛋白相对分子质量约为49 000.分析表明,制备的抗体具有很好的特异性.展开更多
Traditional hand rehabilitation gloves usually use electrical motor as actuator with disadvantages of heaviness,bulkiness and less compliance.Recently,the soft pneumatic actuator is demonstrated to be more suitable fo...Traditional hand rehabilitation gloves usually use electrical motor as actuator with disadvantages of heaviness,bulkiness and less compliance.Recently,the soft pneumatic actuator is demonstrated to be more suitable for hand rehabilitation compared to motor because of its inherent compliance,flexibility and safety.In order to design a wearable glove in request of hand rehabilitation,a soft hoop-reinforced pneumatic actuator is presented.By analyzing the influence of its section shape and geometrical parameters on bending performance,the preferred structure of actuator is achieved based on finite element method.An improved hoop-reinforced actuator is designed after the fabrication and initial measurement,and its mathematical model is built in order to quickly obtain the bending angle response when pressurized.A series of experiment about bending performance are implemented to validate the agreement between the finite element,mathematical and experimental results,and the performance improvement of hoop-reinforced actuator.In addition,the designed hand rehabilitation glove is tested by measuring its output force and actual wearing experience.The output force can reach 2.5 to 3 N when the pressure is 200 kPa.The research results indicate that the designed glove with hoop-reinforced actuator can meet the requirements of hand rehabilitation and has prospective application in hand rehabilitation.展开更多
An underactuated finger structure actuated by tendon-driven system is presented.Kinematics and static analysis of the finger is done,and the results indicate that the prosthetic finger structure is effective and feasi...An underactuated finger structure actuated by tendon-driven system is presented.Kinematics and static analysis of the finger is done,and the results indicate that the prosthetic finger structure is effective and feasible.Based on the design of finger,a prosthetic hand is designed.The hand is composed of 5 independent fingers and it looks more like humanoid.Its size is about 85% of an adult's hand and weights about 350 g.Except the thumb finger,each finger is actuated by one DC motor,gear head and a tendon,and has three curling/extension joints.The thumb finger which is different from other existing prostheses is a novel design scheme.The thumb finger has four joints including three curling/extension joints and a joint which is used to realize the motion of the thumb related to the palm,and these joints are also driven by one DC motor,harmonic drive and a tendon.The underactuation and adaptive curling/extension motion of the finger are realized by joint torsion springs.A high-powered chip of digital signal processing(DSP)is the main part of the electrical system which is used for the motors control,data collection,communication with external controlling source,and so on.To improve the reliability of the hand,structures and sensors are designed and made as simply as possible.The hand has strong manipulation capabilities that have been verified by finger motion and grasping tests and it can satisfy the daily operational needs and psychological needs of deformities.展开更多
A grasping force control strategy is proposed in order to complete various free manipulations by using anthropomorphic prosthetic hand. The position-based impedance control and force-tracking impedance control are use...A grasping force control strategy is proposed in order to complete various free manipulations by using anthropomorphic prosthetic hand. The position-based impedance control and force-tracking impedance control are used in free and constraint spaces, respectively. The fuzzy observer is adopted in transition in order to switch control mode. Two control modes use one position-based impedance controller. In order to achieve grasping force track, reference force is added to the impedance controller in the constraint space. Trajectory tracking in free space and torque tracking in constrained space are realized, and reliability of mode switch and stability of system are achieved. An adaptive sliding mode friction compensation method is proposed. This method makes use of terminal sliding mode idea to design sliding mode function, which makes the tracking error converge to zero in finite time and avoids the problem of conventional sliding surface that tracking error cannot converge to zero. Based on the characteristic of the exponential form friction, the sliding mode control law including the estimation of friction parameter is obtained through terminal sliding mode idea, and the online parameter update laws are obtained based on Lyapunov stability theorem. The experiments on the HIT Prosthetic Hand IV are carried out to evaluate the grasping force control strategy, and the experiment results verify the effectiveness of this control strategy.展开更多
Real-time hand gesture recognition technology significantly improves the user's experience for virtual reality/augmented reality(VR/AR) applications, which relies on the identification of the orientation of the ha...Real-time hand gesture recognition technology significantly improves the user's experience for virtual reality/augmented reality(VR/AR) applications, which relies on the identification of the orientation of the hand in captured images or videos. A new three-stage pipeline approach for fast and accurate hand segmentation for the hand from a single depth image is proposed. Firstly, a depth frame is segmented into several regions by histogrambased threshold selection algorithm and by tracing the exterior boundaries of objects after thresholding. Secondly, each segmentation proposal is evaluated by a three-layers shallow convolutional neural network(CNN) to determine whether or not the boundary is associated with the hand. Finally, all hand components are merged as the hand segmentation result. Compared with algorithms based on random decision forest(RDF), the experimental results demonstrate that the approach achieves better performance with high-accuracy(88.34% mean intersection over union, mIoU) and a shorter processing time(≤8 ms).展开更多
In this paper, the application of bluetooth in vehicle hands free system was described, then the hardware and software structure of system had been designed. Finally, selection about electron parts and chip of electro...In this paper, the application of bluetooth in vehicle hands free system was described, then the hardware and software structure of system had been designed. Finally, selection about electron parts and chip of electronic equipments was presented in detail.展开更多
Previously, researchers raised the accuracy for a robot′s hand to track a specified path in Car-tesian space mainly through increasing the number of knots on the path and the number of the path′s segments, which res...Previously, researchers raised the accuracy for a robot′s hand to track a specified path in Car-tesian space mainly through increasing the number of knots on the path and the number of the path′s segments, which results in the heavier online computational burden for the robot controller. Aiming at overcoming this drawback, the authors propose a new kind of real-time accurate hand path tracking and joint trajectory planning method. Through selecting some extra knots on the specified hand path by a certain rule and introducing a sinusoidal function to the joint displacement equation of each segment, this method can greatly raise the path tracking accuracy of robot′s hand and does not change the number of the path′s segments. It also does not increase markedly the computational burden of robot controller. The result of simulation indicates that this method is very effective, and has important value in increasing the application of industrial robots.展开更多
This paper considers an optimal sequential inspection schedule for a second-hand product after that the free nonrenewable warranty is expired. The length of warranty is prespecified and during the warranty period, the...This paper considers an optimal sequential inspection schedule for a second-hand product after that the free nonrenewable warranty is expired. The length of warranty is prespecified and during the warranty period, the product is minimally repaired by the dealer when it fails. Following the expiration of the non-renewing warranty, the product is inspected and upgraded sequentially a fixed number of times at the expenses of the customer.At each inspection, the failure rate of the product is reduced proportionally so that the product is upgraded. The product is assumed to deteriorate as it ages and the replacement of the product occurs when a fixed number of inspections are rendered. In addition,the intervals between two successive inspections are assumed to decrease monotonically. The main objective of this paper is to determine the optimal improvement level to upgrade the product at each inspection so that the expected maintenance cost during the life cycle of the product is minimized from the perspective of the customer. Under the given cost structures, we derive an explicit formula to obtain the expected maintenance cost incurred during the life cycle of the product and discuss the method to find the optimal level of the improvement analytically in case the failure times follow the Weibull distribution. Numerical results are analyzed to observe the impact of relevant parameters on the optimal solution.展开更多
文摘为利用斑马鱼动物模型进一步研究bHLH转录因子家庭重要成员hand2基因在心脏发育中的功能,采用生物信息学结合RT-PCR的方法获得了斑马鱼hand2基因.将所得片段插入原核表达载体pGEX-4T-1中,并通过IPTG诱导表达出GST-Hand2融合蛋白,经GST亲和层析法纯化后,免疫新西兰大白兔制备了多克隆抗体,利用western b lotting和免疫组化的方法对抗体进行分析.分析表明:斑马鱼hand2基因成熟肽编码区含有627 bp,编码208个氨基酸,与人Hand2蛋白的同源性达到83%;IPTG诱导表达后,表达的融合蛋白占菌体总蛋白的71%,经GST纯化获得SDS-PAGE电泳下单一条带.融合蛋白相对分子质量约为49 000.分析表明,制备的抗体具有很好的特异性.
基金Project(51305202)supported by the National Natural Science Foundation of China
文摘Traditional hand rehabilitation gloves usually use electrical motor as actuator with disadvantages of heaviness,bulkiness and less compliance.Recently,the soft pneumatic actuator is demonstrated to be more suitable for hand rehabilitation compared to motor because of its inherent compliance,flexibility and safety.In order to design a wearable glove in request of hand rehabilitation,a soft hoop-reinforced pneumatic actuator is presented.By analyzing the influence of its section shape and geometrical parameters on bending performance,the preferred structure of actuator is achieved based on finite element method.An improved hoop-reinforced actuator is designed after the fabrication and initial measurement,and its mathematical model is built in order to quickly obtain the bending angle response when pressurized.A series of experiment about bending performance are implemented to validate the agreement between the finite element,mathematical and experimental results,and the performance improvement of hoop-reinforced actuator.In addition,the designed hand rehabilitation glove is tested by measuring its output force and actual wearing experience.The output force can reach 2.5 to 3 N when the pressure is 200 kPa.The research results indicate that the designed glove with hoop-reinforced actuator can meet the requirements of hand rehabilitation and has prospective application in hand rehabilitation.
基金Project(2008AA04Z203)supported by National High Technology Research and Development Program of China
文摘An underactuated finger structure actuated by tendon-driven system is presented.Kinematics and static analysis of the finger is done,and the results indicate that the prosthetic finger structure is effective and feasible.Based on the design of finger,a prosthetic hand is designed.The hand is composed of 5 independent fingers and it looks more like humanoid.Its size is about 85% of an adult's hand and weights about 350 g.Except the thumb finger,each finger is actuated by one DC motor,gear head and a tendon,and has three curling/extension joints.The thumb finger which is different from other existing prostheses is a novel design scheme.The thumb finger has four joints including three curling/extension joints and a joint which is used to realize the motion of the thumb related to the palm,and these joints are also driven by one DC motor,harmonic drive and a tendon.The underactuation and adaptive curling/extension motion of the finger are realized by joint torsion springs.A high-powered chip of digital signal processing(DSP)is the main part of the electrical system which is used for the motors control,data collection,communication with external controlling source,and so on.To improve the reliability of the hand,structures and sensors are designed and made as simply as possible.The hand has strong manipulation capabilities that have been verified by finger motion and grasping tests and it can satisfy the daily operational needs and psychological needs of deformities.
基金Project(2009AA043803) supported by the National High Technology Research and Development Program of China Project (SKLRS200901B) supported by Self-Planned Task of State Key Laboratory of Robotics and System (Harbin Institute of Technology),ChinaProject (NCET-09-0056) supported by Program for New Century Excellent Talents in Universities of China
文摘A grasping force control strategy is proposed in order to complete various free manipulations by using anthropomorphic prosthetic hand. The position-based impedance control and force-tracking impedance control are used in free and constraint spaces, respectively. The fuzzy observer is adopted in transition in order to switch control mode. Two control modes use one position-based impedance controller. In order to achieve grasping force track, reference force is added to the impedance controller in the constraint space. Trajectory tracking in free space and torque tracking in constrained space are realized, and reliability of mode switch and stability of system are achieved. An adaptive sliding mode friction compensation method is proposed. This method makes use of terminal sliding mode idea to design sliding mode function, which makes the tracking error converge to zero in finite time and avoids the problem of conventional sliding surface that tracking error cannot converge to zero. Based on the characteristic of the exponential form friction, the sliding mode control law including the estimation of friction parameter is obtained through terminal sliding mode idea, and the online parameter update laws are obtained based on Lyapunov stability theorem. The experiments on the HIT Prosthetic Hand IV are carried out to evaluate the grasping force control strategy, and the experiment results verify the effectiveness of this control strategy.
文摘Real-time hand gesture recognition technology significantly improves the user's experience for virtual reality/augmented reality(VR/AR) applications, which relies on the identification of the orientation of the hand in captured images or videos. A new three-stage pipeline approach for fast and accurate hand segmentation for the hand from a single depth image is proposed. Firstly, a depth frame is segmented into several regions by histogrambased threshold selection algorithm and by tracing the exterior boundaries of objects after thresholding. Secondly, each segmentation proposal is evaluated by a three-layers shallow convolutional neural network(CNN) to determine whether or not the boundary is associated with the hand. Finally, all hand components are merged as the hand segmentation result. Compared with algorithms based on random decision forest(RDF), the experimental results demonstrate that the approach achieves better performance with high-accuracy(88.34% mean intersection over union, mIoU) and a shorter processing time(≤8 ms).
文摘In this paper, the application of bluetooth in vehicle hands free system was described, then the hardware and software structure of system had been designed. Finally, selection about electron parts and chip of electronic equipments was presented in detail.
基金Foundation of the Robotics Laboratory, Chinese Academy of Sciences (No: RL200002)
文摘Previously, researchers raised the accuracy for a robot′s hand to track a specified path in Car-tesian space mainly through increasing the number of knots on the path and the number of the path′s segments, which results in the heavier online computational burden for the robot controller. Aiming at overcoming this drawback, the authors propose a new kind of real-time accurate hand path tracking and joint trajectory planning method. Through selecting some extra knots on the specified hand path by a certain rule and introducing a sinusoidal function to the joint displacement equation of each segment, this method can greatly raise the path tracking accuracy of robot′s hand and does not change the number of the path′s segments. It also does not increase markedly the computational burden of robot controller. The result of simulation indicates that this method is very effective, and has important value in increasing the application of industrial robots.
基金supported by the Research Base Construction Fund Support Program funded by Chonbuk National University in 2013the Mid-career Research Program(2016R1A2B4010080)through NRF Grant funded by MEST
文摘This paper considers an optimal sequential inspection schedule for a second-hand product after that the free nonrenewable warranty is expired. The length of warranty is prespecified and during the warranty period, the product is minimally repaired by the dealer when it fails. Following the expiration of the non-renewing warranty, the product is inspected and upgraded sequentially a fixed number of times at the expenses of the customer.At each inspection, the failure rate of the product is reduced proportionally so that the product is upgraded. The product is assumed to deteriorate as it ages and the replacement of the product occurs when a fixed number of inspections are rendered. In addition,the intervals between two successive inspections are assumed to decrease monotonically. The main objective of this paper is to determine the optimal improvement level to upgrade the product at each inspection so that the expected maintenance cost during the life cycle of the product is minimized from the perspective of the customer. Under the given cost structures, we derive an explicit formula to obtain the expected maintenance cost incurred during the life cycle of the product and discuss the method to find the optimal level of the improvement analytically in case the failure times follow the Weibull distribution. Numerical results are analyzed to observe the impact of relevant parameters on the optimal solution.
