For this paper, the plasma membrane （PM） H^＋-ATPase gene has been cloned from Populus euphratica Oliv. through a ho- mology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame ...For this paper, the plasma membrane （PM） H^＋-ATPase gene has been cloned from Populus euphratica Oliv. through a ho- mology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame （ORF） which encodes a putative H^＋-ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H^＋-ATPase of Prunus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H^＋-ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeI, FbaI and Bg/Ⅱ, and Southern blot.展开更多
文摘For this paper, the plasma membrane （PM） H^＋-ATPase gene has been cloned from Populus euphratica Oliv. through a ho- mology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame （ORF） which encodes a putative H^＋-ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H^＋-ATPase of Prunus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H^＋-ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeI, FbaI and Bg/Ⅱ, and Southern blot.
