Objective: To study the effect of vancomycin (V) with multiple intravenous injections on the inner ear of albino guinea pigs. Methods: Three groups of animals were injected with vancomycin hydrochloride (54, 108, 216 ...Objective: To study the effect of vancomycin (V) with multiple intravenous injections on the inner ear of albino guinea pigs. Methods: Three groups of animals were injected with vancomycin hydrochloride (54, 108, 216 mg/kg respectively once a day for 14 d). Two groups were treated with gentamycin (GM) (80 mg·kg -1 ·d -1 ) and saline respectively as control groups. Auditory brainstem responses (ABR) and the duration of post-rotatory nystagmus (PRN) were measured before and after administration. Surface preparation and scanning electron microscopy (SEM) of the cochlea were performed for histological examination. Results: In V 54, 108 mg/kg group, similar to saline control, there was 0-1.1 dB of threshold shift. In V 216 mg/kg group, average hearing loss was 1.0-5.7 dB immediately after administration and 1.3-3.8 dB after 14 d, which was significantly lower than those in GM control group. As the saline control, no significant difference was found in PRN in all V groups before and after treatment; while in the GM group, PRN decreased significantly after treatment. Morphological evaluation demonstrated that in all V and saline animals there was no obvious missing of outer and inner hair cells and SEM showed normal surface morphology. In the GM group, there was 10%-30% of outer and inner hair cells lost in the basal turn. Conclusion: The ototoxicity of vancomycin is absent or minimal after multiple introvenous administration within this dose range.展开更多
文摘Objective: To study the effect of vancomycin (V) with multiple intravenous injections on the inner ear of albino guinea pigs. Methods: Three groups of animals were injected with vancomycin hydrochloride (54, 108, 216 mg/kg respectively once a day for 14 d). Two groups were treated with gentamycin (GM) (80 mg·kg -1 ·d -1 ) and saline respectively as control groups. Auditory brainstem responses (ABR) and the duration of post-rotatory nystagmus (PRN) were measured before and after administration. Surface preparation and scanning electron microscopy (SEM) of the cochlea were performed for histological examination. Results: In V 54, 108 mg/kg group, similar to saline control, there was 0-1.1 dB of threshold shift. In V 216 mg/kg group, average hearing loss was 1.0-5.7 dB immediately after administration and 1.3-3.8 dB after 14 d, which was significantly lower than those in GM control group. As the saline control, no significant difference was found in PRN in all V groups before and after treatment; while in the GM group, PRN decreased significantly after treatment. Morphological evaluation demonstrated that in all V and saline animals there was no obvious missing of outer and inner hair cells and SEM showed normal surface morphology. In the GM group, there was 10%-30% of outer and inner hair cells lost in the basal turn. Conclusion: The ototoxicity of vancomycin is absent or minimal after multiple introvenous administration within this dose range.
