Background Unravelling the relationship between trichome density and resistance to jassids in upland cotton,nine parental lines,viz.MCU 5,CO 14,CO 17,TCH 1828,KC 2,KC 3,GISV 323,GTHV 15–34,and RHC 1409 were obtained ...Background Unravelling the relationship between trichome density and resistance to jassids in upland cotton,nine parental lines,viz.MCU 5,CO 14,CO 17,TCH 1828,KC 2,KC 3,GISV 323,GTHV 15–34,and RHC 1409 were obtained from the Tamilnadu Agricultural University.These genotypes were subjected to molecular analysis using 27 primers,merely the JESPR 154 primer amplifying a 150-bp fragment in genotypes exhibiting the pubescence.Result This finding validated the association between pubescence and jassid resistance.Further analysis revealed that resistant genotypes(KC 3,GTHV 15–34,GISV 323,and RHC 1409)exhibited significantly higher trichome densities and length compared with susceptible genotypes.These results stalwartly support the hypothesis that trichomes play a pivotal role in conferring resistance to jassids in upland cotton.Conclusion By breeding cotton varieties with increased trichome density and length,it is possible to reduce jassid infestations,thereby decreasing the reliance on chemical pesticides and promoting a more sustainable agricultural environment.展开更多
Correction:J Cotton Res 8,27(2025)https://doi.org/10.1186/s42397-025-00228-y During the publication process of the original article(Soltani Toularoud et al.2025),the article title has been wrongly captured.Te article ...Correction:J Cotton Res 8,27(2025)https://doi.org/10.1186/s42397-025-00228-y During the publication process of the original article(Soltani Toularoud et al.2025),the article title has been wrongly captured.Te article title should be corrected from:of butisanstar and clopyralid herbicides on Gos-sypium hirsutum L.growth:insights from a pot experiment to:Residual efects of butisanstar and clopyralid herbi-cides on Gossypium hirsutum L.growth:insights from a pot experiment Te original article(Soltani Toularoud et al.2025)has been updated.Te publisher apologizes to the authors and readers for the inconvenience caused.展开更多
Background Mepiquat chloride(MC)is a widely used plant growth regulator in cotton(Gossypium hirsutum L.).It regulates endogenous hormone content and crosstalk to control plant height and promote lateral root(LR)develo...Background Mepiquat chloride(MC)is a widely used plant growth regulator in cotton(Gossypium hirsutum L.).It regulates endogenous hormone content and crosstalk to control plant height and promote lateral root(LR)development.However,the roles of cytokinins(CTKs)in the MC-induced increase in LR number in cotton seedlings remain unclear.Therefore,in this study,whole-genome transcriptome analysis was performed to elucidate the molecular mechanisms,CTK transformation,and CTK signaling pathway response to MC in cotton roots.Results In the present study,MC reduced the contents of the active CTK trans-zeatin(tZ)and N^(6)-isopentenyladenine(iP)but increased the levels of the nucleoside CTK trans-zeatin riboside(tZR)and N^(6)-isopentenyladenine riboside(iPR).RNA-seq data showed that the CTK biosynthesis genes GhIPTs and active CTK catabolism genes GhCKXs were obviously upregulated after MC treatment.The CTK-activating enzyme gene GhLOGs was repressed compared with the control.Furthermore,MC inhibited the expression of GhAHK4 and GhARR2/12,which are involved in the CTK signaling pathway,and activated the IAA-IAA14-ARF7/19 signaling module.Meanwhile,MC increased the expression levels of genes involved in sucrose synthesis,the cell cycle,cell division,and cell wall biosynthesis pathways.Silencing the GhCKX family separately decreased the LR number and active indole-3-acetic acid(IAA)level.The expression levels of GhPIN1,GhARF7,GhARF19,GhLBD16,GhLBD18,GhLBD29,and GhLBD33 were downregulated,but GhARR2/12 and GhIAA14 were upregulated.The total content of active CTKs was noticeably increased.The results of silencing the GhLOGs family were opposite to those of silencing GhCKXs.Silencing GhARR12 could upregulate GhPIN1 expression and increase LR number.In addition,the silenced GhCKXs,GhLOGs,and GhARR12 were less responsive to MCinduced LR growth than the control.Conclusion These results suggested that MC treatment could upregulate CTK-nucleoside biosynthesis and CTK metabolism genes to decrease active CTK levels,promoting crosstalk between CTKs and auxin signaling pathways to enhance LR initiation.展开更多
Background Gossypium barbadense L.has specific fibre in terms of its length,strength,and fineness,and known as extra-long staple(ELS) cotton,Sea–Island cotton,or Egyptian cotton.Narrow genetic base with less genetic ...Background Gossypium barbadense L.has specific fibre in terms of its length,strength,and fineness,and known as extra-long staple(ELS) cotton,Sea–Island cotton,or Egyptian cotton.Narrow genetic base with less genetic variability is observed in G.barbadense germplasm.Hence,this study was aimed to evaluate the genetic variability present in 108 germplasm accessions of G.barbadense and to identify the superior genotypes based on the fibre traits.Results We evaluated 108 accessions for five fibre quality traits along with three checks in augmented block design.All fibre traits showed significant differences among genotypes,indicating that there is genetic potential for improvement.Fibre strength and micronaire(MIC) showed high phenotypic and genotypic coefficients of variation.High heritability combined with high genetic advance as percentage of mean(GAM) was recorded for fibre length,strength,and micronaire.Fibre strength and fibre length were significantly correlated with each other,while both showed negative correlation with micronaire.Principal component analysis and Biplot analysis showed that uniformity index discriminated all the genotypes in higher level,while fibre length and strength were medium in discrimination power.Biplot revealed genotypes DB 16,EC959191,GSB 39,ARBB 20,5746U,EA 203,and EA 201 were genetically diverse.Hierarchal cluster analysis based on unweighted paired group method using arithmetic average(UPGMA) grouped the genotypes into four clusters,with each cluster consisting of 4,18,48,and 38 genotypes,respectively.Conclusion Among the genotypes,34 for fibre length(> 35 mm),18 for fibre strength(> 40.4 g·tex^(-1)) and 66 for micronaire(3.7-4.2,A grade) were identified as potential accessions based on their superiority.The superior fibre genotypes identified in this study are potential lines for the ELS cotton breeding program.展开更多
Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cyt...Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cytogenetics, and genomics. Here we update and provide a brief summary of the emerging picture of species relationships and diversification, and a set of the designations for individual genomes and chromosomes in Gossypium. This cytogenetic and genomic nomenclature will facilitate comparative studies worldwide, which range from basic taxonomic exploration to breeding and germplasm introgression.展开更多
Background： RING H2 finger E3 ligase （RH2FE3） genes encode cysteine rich proteins that mediate E3 ubiquitin ligase activity and degrade target substrates. The roles of these genes in plant responses to phytohormone...Background： RING H2 finger E3 ligase （RH2FE3） genes encode cysteine rich proteins that mediate E3 ubiquitin ligase activity and degrade target substrates. The roles of these genes in plant responses to phytohormones and abiotic stresses are well documented in various species, but their roles in cotton fiber development are poorly understood. To date, genome wide identification and expression analyses of Gossypium hirsutum RH2FE3 genes have not been reported. Methods： We performed computational identification, structural and phylogenetic analyses, chromosomal distribution analysis and estimated KJKs values of G hirsutum RH2FE3 genes. Orthologous and paralogous gene pairs were identified by all versus all BLASTP searches. We predicted cis regulatory elements and analyzed microarray data sets to generate heatmaps at different development stages. Tissue specific expression in cotton fiber, and hormonal and abiotic stress responses were determined by quantitative real time polymerase chain reaction （qRT PCR） analysis. Results： We investigated 140 G hirsutum, 80 G. orboreum, and evolutionary mechanisms and compared them with orthologs 89 G. roimondii putative RH2FB genes and their in Arobidopsis and rice. A domain based analysis of the G hirsutum RH2FE3 genes predicted conserved signature motifs and gene structures. Chromosomal localization showed the genes were distributed across all G hirsutum chromosomes, and 60 duplication events （4 tandem and 56 segmental duplications） and 98 orthologs were detected, cis elements were detected in the promoter regions of G hirsutum RH2FE3 genes. Microarray data and qRT PCR analyses showed that G hirsutum RH2FE3 genes were strongly correlated with cotton fiber development. Additionally, almost all the （brassinolide, gibberellic acid （GA）, indole 3-acetic acid drought, and salt）. dentified genes were up regulated in response to phytohormones （IAA）, and salicylic acid （SA）） and abiotic stresses （cold, heat, Conclusions： The genome wide identification, comprehensive analysis, and characterization of conserved domains and gene structures, as well as phylogenetic analysis, cis element prediction, and expression profile analysis of G hirsutum RH2FE3 genes and their roles in cotton fiber development and responses to plant hormones and abiotic stresses are reported here for the first time. Our findings will contribute to the genome wide analysis of putative RH2FE3 genes in other species and lay a foundation for future physiological and functional research on G hirsutum RH2FE3 genes.展开更多
Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of salinity.Soil salt-alkalization leads to dam...Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of salinity.Soil salt-alkalization leads to damage in cotton growth and a decrease in yields.Hyperosmolality-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium concentration.Hyperosmolality-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in plants.However,the molecular nature of the corresponding calcium ion channels remains unclearly.In this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse genetics.Result:A total of 35,21 and 22 OSCA genes were identified in G.hirsutum,G.arboreum,and G.raimondii genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic relationship.Gene and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) domain.Moreover,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic stress.Furthermore,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed plants.Conclusion:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in cotton.These results provide a new insight of defense responses in general and lay the foundation for further investigation of the molecular role played by the OSCA genes,thereby providing suitable approaches to improve crop performance under salinity and drought stress conditions.展开更多
The heterosis in cotton is much significant,especially in increasing yield andfiber quality.Comparing with hand-emasculation andpollination,and genetic male sterile lines,utilization of CMS lines is much more effectiv...The heterosis in cotton is much significant,especially in increasing yield andfiber quality.Comparing with hand-emasculation andpollination,and genetic male sterile lines,utilization of CMS lines is much more effectiveand economical in producing commercial hybridseeds.Since 1965 in the world,several CMSlines have been developed,such as CMS展开更多
Verticillium wilt is a global important disease ofcotton,which threatens the development ofcotton production seriously.Recent years,because of the change in climate and croppingpattern,Verticillium wilt was broke out ...Verticillium wilt is a global important disease ofcotton,which threatens the development ofcotton production seriously.Recent years,because of the change in climate and croppingpattern,Verticillium wilt was broke out incotton production areas in China,which展开更多
文摘Background Unravelling the relationship between trichome density and resistance to jassids in upland cotton,nine parental lines,viz.MCU 5,CO 14,CO 17,TCH 1828,KC 2,KC 3,GISV 323,GTHV 15–34,and RHC 1409 were obtained from the Tamilnadu Agricultural University.These genotypes were subjected to molecular analysis using 27 primers,merely the JESPR 154 primer amplifying a 150-bp fragment in genotypes exhibiting the pubescence.Result This finding validated the association between pubescence and jassid resistance.Further analysis revealed that resistant genotypes(KC 3,GTHV 15–34,GISV 323,and RHC 1409)exhibited significantly higher trichome densities and length compared with susceptible genotypes.These results stalwartly support the hypothesis that trichomes play a pivotal role in conferring resistance to jassids in upland cotton.Conclusion By breeding cotton varieties with increased trichome density and length,it is possible to reduce jassid infestations,thereby decreasing the reliance on chemical pesticides and promoting a more sustainable agricultural environment.
文摘Correction:J Cotton Res 8,27(2025)https://doi.org/10.1186/s42397-025-00228-y During the publication process of the original article(Soltani Toularoud et al.2025),the article title has been wrongly captured.Te article title should be corrected from:of butisanstar and clopyralid herbicides on Gos-sypium hirsutum L.growth:insights from a pot experiment to:Residual efects of butisanstar and clopyralid herbi-cides on Gossypium hirsutum L.growth:insights from a pot experiment Te original article(Soltani Toularoud et al.2025)has been updated.Te publisher apologizes to the authors and readers for the inconvenience caused.
基金supported by the National Natural Science Foundation of China(Grant No.31471434)。
文摘Background Mepiquat chloride(MC)is a widely used plant growth regulator in cotton(Gossypium hirsutum L.).It regulates endogenous hormone content and crosstalk to control plant height and promote lateral root(LR)development.However,the roles of cytokinins(CTKs)in the MC-induced increase in LR number in cotton seedlings remain unclear.Therefore,in this study,whole-genome transcriptome analysis was performed to elucidate the molecular mechanisms,CTK transformation,and CTK signaling pathway response to MC in cotton roots.Results In the present study,MC reduced the contents of the active CTK trans-zeatin(tZ)and N^(6)-isopentenyladenine(iP)but increased the levels of the nucleoside CTK trans-zeatin riboside(tZR)and N^(6)-isopentenyladenine riboside(iPR).RNA-seq data showed that the CTK biosynthesis genes GhIPTs and active CTK catabolism genes GhCKXs were obviously upregulated after MC treatment.The CTK-activating enzyme gene GhLOGs was repressed compared with the control.Furthermore,MC inhibited the expression of GhAHK4 and GhARR2/12,which are involved in the CTK signaling pathway,and activated the IAA-IAA14-ARF7/19 signaling module.Meanwhile,MC increased the expression levels of genes involved in sucrose synthesis,the cell cycle,cell division,and cell wall biosynthesis pathways.Silencing the GhCKX family separately decreased the LR number and active indole-3-acetic acid(IAA)level.The expression levels of GhPIN1,GhARF7,GhARF19,GhLBD16,GhLBD18,GhLBD29,and GhLBD33 were downregulated,but GhARR2/12 and GhIAA14 were upregulated.The total content of active CTKs was noticeably increased.The results of silencing the GhLOGs family were opposite to those of silencing GhCKXs.Silencing GhARR12 could upregulate GhPIN1 expression and increase LR number.In addition,the silenced GhCKXs,GhLOGs,and GhARR12 were less responsive to MCinduced LR growth than the control.Conclusion These results suggested that MC treatment could upregulate CTK-nucleoside biosynthesis and CTK metabolism genes to decrease active CTK levels,promoting crosstalk between CTKs and auxin signaling pathways to enhance LR initiation.
基金supported by ICAR-Central Institute for Cotton Research, Regional Station, Coimbatore, India。
文摘Background Gossypium barbadense L.has specific fibre in terms of its length,strength,and fineness,and known as extra-long staple(ELS) cotton,Sea–Island cotton,or Egyptian cotton.Narrow genetic base with less genetic variability is observed in G.barbadense germplasm.Hence,this study was aimed to evaluate the genetic variability present in 108 germplasm accessions of G.barbadense and to identify the superior genotypes based on the fibre traits.Results We evaluated 108 accessions for five fibre quality traits along with three checks in augmented block design.All fibre traits showed significant differences among genotypes,indicating that there is genetic potential for improvement.Fibre strength and micronaire(MIC) showed high phenotypic and genotypic coefficients of variation.High heritability combined with high genetic advance as percentage of mean(GAM) was recorded for fibre length,strength,and micronaire.Fibre strength and fibre length were significantly correlated with each other,while both showed negative correlation with micronaire.Principal component analysis and Biplot analysis showed that uniformity index discriminated all the genotypes in higher level,while fibre length and strength were medium in discrimination power.Biplot revealed genotypes DB 16,EC959191,GSB 39,ARBB 20,5746U,EA 203,and EA 201 were genetically diverse.Hierarchal cluster analysis based on unweighted paired group method using arithmetic average(UPGMA) grouped the genotypes into four clusters,with each cluster consisting of 4,18,48,and 38 genotypes,respectively.Conclusion Among the genotypes,34 for fibre length(> 35 mm),18 for fibre strength(> 40.4 g·tex^(-1)) and 66 for micronaire(3.7-4.2,A grade) were identified as potential accessions based on their superiority.The superior fibre genotypes identified in this study are potential lines for the ELS cotton breeding program.
基金National Natural Science Foundation of China(31530053)
文摘Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cytogenetics, and genomics. Here we update and provide a brief summary of the emerging picture of species relationships and diversification, and a set of the designations for individual genomes and chromosomes in Gossypium. This cytogenetic and genomic nomenclature will facilitate comparative studies worldwide, which range from basic taxonomic exploration to breeding and germplasm introgression.
基金supported by the Major Research Plan of National Natural Science Foundation of China(NO.31690093)Young Elite Scientist Sponsorship Program by CAST(China Association for Science and Technology)
文摘Background： RING H2 finger E3 ligase （RH2FE3） genes encode cysteine rich proteins that mediate E3 ubiquitin ligase activity and degrade target substrates. The roles of these genes in plant responses to phytohormones and abiotic stresses are well documented in various species, but their roles in cotton fiber development are poorly understood. To date, genome wide identification and expression analyses of Gossypium hirsutum RH2FE3 genes have not been reported. Methods： We performed computational identification, structural and phylogenetic analyses, chromosomal distribution analysis and estimated KJKs values of G hirsutum RH2FE3 genes. Orthologous and paralogous gene pairs were identified by all versus all BLASTP searches. We predicted cis regulatory elements and analyzed microarray data sets to generate heatmaps at different development stages. Tissue specific expression in cotton fiber, and hormonal and abiotic stress responses were determined by quantitative real time polymerase chain reaction （qRT PCR） analysis. Results： We investigated 140 G hirsutum, 80 G. orboreum, and evolutionary mechanisms and compared them with orthologs 89 G. roimondii putative RH2FB genes and their in Arobidopsis and rice. A domain based analysis of the G hirsutum RH2FE3 genes predicted conserved signature motifs and gene structures. Chromosomal localization showed the genes were distributed across all G hirsutum chromosomes, and 60 duplication events （4 tandem and 56 segmental duplications） and 98 orthologs were detected, cis elements were detected in the promoter regions of G hirsutum RH2FE3 genes. Microarray data and qRT PCR analyses showed that G hirsutum RH2FE3 genes were strongly correlated with cotton fiber development. Additionally, almost all the （brassinolide, gibberellic acid （GA）, indole 3-acetic acid drought, and salt）. dentified genes were up regulated in response to phytohormones （IAA）, and salicylic acid （SA）） and abiotic stresses （cold, heat, Conclusions： The genome wide identification, comprehensive analysis, and characterization of conserved domains and gene structures, as well as phylogenetic analysis, cis element prediction, and expression profile analysis of G hirsutum RH2FE3 genes and their roles in cotton fiber development and responses to plant hormones and abiotic stresses are reported here for the first time. Our findings will contribute to the genome wide analysis of putative RH2FE3 genes in other species and lay a foundation for future physiological and functional research on G hirsutum RH2FE3 genes.
基金funded by the National Natural Science Foundation of China(31530053/31621005)the National Key R&D Program(2016YFD0101401/2017YFD0101601)
文摘Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of salinity.Soil salt-alkalization leads to damage in cotton growth and a decrease in yields.Hyperosmolality-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium concentration.Hyperosmolality-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in plants.However,the molecular nature of the corresponding calcium ion channels remains unclearly.In this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse genetics.Result:A total of 35,21 and 22 OSCA genes were identified in G.hirsutum,G.arboreum,and G.raimondii genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic relationship.Gene and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) domain.Moreover,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic stress.Furthermore,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed plants.Conclusion:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in cotton.These results provide a new insight of defense responses in general and lay the foundation for further investigation of the molecular role played by the OSCA genes,thereby providing suitable approaches to improve crop performance under salinity and drought stress conditions.
文摘The heterosis in cotton is much significant,especially in increasing yield andfiber quality.Comparing with hand-emasculation andpollination,and genetic male sterile lines,utilization of CMS lines is much more effectiveand economical in producing commercial hybridseeds.Since 1965 in the world,several CMSlines have been developed,such as CMS
文摘Verticillium wilt is a global important disease ofcotton,which threatens the development ofcotton production seriously.Recent years,because of the change in climate and croppingpattern,Verticillium wilt was broke out incotton production areas in China,which
