Trehalose synthase is an important functional enzyme in the synthesis of trehalose in organisms and also participates in plant stress-resistant physiological processes.The transcriptomic study showed that a trehalose-...Trehalose synthase is an important functional enzyme in the synthesis of trehalose in organisms and also participates in plant stress-resistant physiological processes.The transcriptomic study showed that a trehalose-6-phosphate synthase gene was responsive to salt and alkaline stresses in Glycine soja.To dissect the molecular mechanisms of this enzyme in plant responses to stresses,the PCR technique was used to clone a trehalose-6-phosphate synthase gene from Glycine soja and it was designated as the GsTPS9.The full-length cDNA of this gene was 2583bp which encoded 861 amino acids.The sequence and structure analyses indicated that the GsTPS9 had high homology with Glycine max GmTPS9.The qRT-PCR analysis revealed that the GsTPS9 gene was expressed in Glycine soja roots,stems and leaves,and the highest expression level was in roots;the GsTPS9 gene had different responses under the stresses of NaCl,NaHCO_(3),PEG6000,ABA,MeJA and SA.This study laid the foundation for revealing the mechanism of the TPS in plant signal transduction pathways.展开更多
The enzyme myo-inositol-1-phosphate synthase（MIPS EC 5.5.1.4） catalyzes the first step of myo-inositol biosynthesis, a product that plays crucial roles in plants as an osmoprotectant, transduction molecule, cell wal...The enzyme myo-inositol-1-phosphate synthase（MIPS EC 5.5.1.4） catalyzes the first step of myo-inositol biosynthesis, a product that plays crucial roles in plants as an osmoprotectant, transduction molecule, cell wall constituent and production of stress related molecule. Previous reports highlighted an important role of MIPS family genes in abiotic stresses particularly under salt stress tolerance in several plant species; however, little is known about the cellular and physiological functions of MIPS2 genes under abiotic conditions. In this study, a novel salt stress responsive gene designated Gs MIPS2 from wild soybean Glycine soja 07256 was functionally characterized contained an open reading frame（ORF） of 1 533 bp coding a peptide sequence of 510 amino acids along with mass of 56 445 ku. Multiple sequence alignment analysis revealed its 92%-99% similarity with other MIPS family members in legume proteins. Quantitative real-time PCR results demonstrated that Gs MIPS2 was induced by salt stress and expressed in roots of soybean. The positive function of Gs MIPS2 under salt response at different growth stages of transgenic Arabidopsis was also elucidated. The results showed that Gs MIPS2 transgenic lines displayed increased tolerance as compared to WT and atmips2 mutant lines under salt stress. Furthermore, the expression levels of some salt stress responsive marker genes, including KIN1, RD29 A, RD29 B, P5 Cs and COR47 were significantly up-regulated in Gs MIPS2 overexpression lines than wild type and atmips2 mutant. Collectively, these results suggested that Gs MIPS2 gene was a positive regulator of plant tolerance to salt stress. This was the first report to demonstrate that overexpression of Gs MIPS2 gene from wild soybean improved salt tolerance in transgenic Arabidopsis.展开更多
S-adenosylmethionine （SAM） plays important role in trans-methyl reactions. Under the condition of drought （30% PEG）, salinity （200 mmol· L^-1 NaCl） and low temperature （4℃）, total RNA was extracted from ...S-adenosylmethionine （SAM） plays important role in trans-methyl reactions. Under the condition of drought （30% PEG）, salinity （200 mmol· L^-1 NaCl） and low temperature （4℃）, total RNA was extracted from the leaf and the first strand of cDNA was synthesized with reverse transcription. S-adenosylmethionine synthetase gene （SAMS gene） was amplified by PCR with the first strand cDNA as template and a pair of primers which was based on constructed ESTs sequence. Full-length SAMS gene sequence was obtained by BLAST comparison. According to the analysis, completed sequence of SAMS gene was integrality. The sequence of the SAMS gene was 1 185 bp in length with an opening reading frame （ORF） encoding 394 amino acids. The cDNA sequence showed a significant homology to the SAM genes from Phaseolus lunatus （89%）, Medicago sativa （85%）. A prokaryotic expression vectors based on pET-32b had been constructed and prokaryotic expression was analyzed in order to lay a strong foundation for resist adversity function analysis through situation of genic expression analysis.展开更多
基金Supported by the National Natural Science Foundation of China(31670272)Heilongjiang Provincial Natural Science Foundation(C2017014)。
文摘Trehalose synthase is an important functional enzyme in the synthesis of trehalose in organisms and also participates in plant stress-resistant physiological processes.The transcriptomic study showed that a trehalose-6-phosphate synthase gene was responsive to salt and alkaline stresses in Glycine soja.To dissect the molecular mechanisms of this enzyme in plant responses to stresses,the PCR technique was used to clone a trehalose-6-phosphate synthase gene from Glycine soja and it was designated as the GsTPS9.The full-length cDNA of this gene was 2583bp which encoded 861 amino acids.The sequence and structure analyses indicated that the GsTPS9 had high homology with Glycine max GmTPS9.The qRT-PCR analysis revealed that the GsTPS9 gene was expressed in Glycine soja roots,stems and leaves,and the highest expression level was in roots;the GsTPS9 gene had different responses under the stresses of NaCl,NaHCO_(3),PEG6000,ABA,MeJA and SA.This study laid the foundation for revealing the mechanism of the TPS in plant signal transduction pathways.
基金Supported by "863" Project(2008AA10Z153)the National Natural Science Foundation of China(31171578)+1 种基金Heilongjiang Provincial Higher School Science and Technology Innovation Team Building Program(2011TD005)the National Basic Scientific Talent Training Fund Projects(J1210069)
文摘The enzyme myo-inositol-1-phosphate synthase（MIPS EC 5.5.1.4） catalyzes the first step of myo-inositol biosynthesis, a product that plays crucial roles in plants as an osmoprotectant, transduction molecule, cell wall constituent and production of stress related molecule. Previous reports highlighted an important role of MIPS family genes in abiotic stresses particularly under salt stress tolerance in several plant species; however, little is known about the cellular and physiological functions of MIPS2 genes under abiotic conditions. In this study, a novel salt stress responsive gene designated Gs MIPS2 from wild soybean Glycine soja 07256 was functionally characterized contained an open reading frame（ORF） of 1 533 bp coding a peptide sequence of 510 amino acids along with mass of 56 445 ku. Multiple sequence alignment analysis revealed its 92%-99% similarity with other MIPS family members in legume proteins. Quantitative real-time PCR results demonstrated that Gs MIPS2 was induced by salt stress and expressed in roots of soybean. The positive function of Gs MIPS2 under salt response at different growth stages of transgenic Arabidopsis was also elucidated. The results showed that Gs MIPS2 transgenic lines displayed increased tolerance as compared to WT and atmips2 mutant lines under salt stress. Furthermore, the expression levels of some salt stress responsive marker genes, including KIN1, RD29 A, RD29 B, P5 Cs and COR47 were significantly up-regulated in Gs MIPS2 overexpression lines than wild type and atmips2 mutant. Collectively, these results suggested that Gs MIPS2 gene was a positive regulator of plant tolerance to salt stress. This was the first report to demonstrate that overexpression of Gs MIPS2 gene from wild soybean improved salt tolerance in transgenic Arabidopsis.
基金National Science Foundation (30570990)Heilongjiang Province Educational Committee Science Research Foundation (11521023)
文摘S-adenosylmethionine （SAM） plays important role in trans-methyl reactions. Under the condition of drought （30% PEG）, salinity （200 mmol· L^-1 NaCl） and low temperature （4℃）, total RNA was extracted from the leaf and the first strand of cDNA was synthesized with reverse transcription. S-adenosylmethionine synthetase gene （SAMS gene） was amplified by PCR with the first strand cDNA as template and a pair of primers which was based on constructed ESTs sequence. Full-length SAMS gene sequence was obtained by BLAST comparison. According to the analysis, completed sequence of SAMS gene was integrality. The sequence of the SAMS gene was 1 185 bp in length with an opening reading frame （ORF） encoding 394 amino acids. The cDNA sequence showed a significant homology to the SAM genes from Phaseolus lunatus （89%）, Medicago sativa （85%）. A prokaryotic expression vectors based on pET-32b had been constructed and prokaryotic expression was analyzed in order to lay a strong foundation for resist adversity function analysis through situation of genic expression analysis.
