Two types of markers-random amplified polymorphic DNA （RAPD） and simple sequence repeat DNA （SSR）-have been used to characterize the genetic diversity among nine mutant lines of transgenic wheat intermediated by l...Two types of markers-random amplified polymorphic DNA （RAPD） and simple sequence repeat DNA （SSR）-have been used to characterize the genetic diversity among nine mutant lines of transgenic wheat intermediated by low energy ion beam and their four receptor cultivars. The objectives of this study were to analyze RAPD-based and SSR-based genetic variance among transgenic wheat lines and with their receptors, and to find specific genetic markers of special traits of transgenic wheat lines. 170 RAPD primers were amplified to 733 fragments in all the experimental materials. There were 121 polymorphic fragments out of the 733 fragments with a ratio of polymorphic fragments of 16.5%. 29 SSR primer pairs were amplified to 83 fragments in all the experiment materials. There were 57 polymorphic fragments out of the 83 fragments with a ratio of polymorphic fragments of 68.7%. The dendrograms were prepared based on a genetic distance matrix using the UPGMA （Unweighted Pair-group Method with Arithmetic averaging） algorithm, which corresponded well to the results of the wheat pedigree analysis and separated the 13 genotypes into four groups. Association analysis between RAPD and SSR markers with the special traits of transgenic wheat mutant lines discovered that three RAPD markers, sl, opt- 16, and f14, were significantly associated with the muticate trait, while three SSR markers, Rht8 （Xgwm261）, Rht-Blb, and Rht-Dlb, highly associated with the dwarf trait. These markers will be useful for marker-assistant breeding and can be used as candidate markers for further gene mapping and cloning.展开更多
Objective There is little population-based data on the prevalence and the environmental or genetic determinants of left ventricular hypertrophy （LVH） in China. The purpose of this paper is to study LVH in relation t...Objective There is little population-based data on the prevalence and the environmental or genetic determinants of left ventricular hypertrophy （LVH） in China. The purpose of this paper is to study LVH in relation to systolic blood pressure and the angiotensin converting enzyme （ACE） insertion/deletion（I/D） polymorphism in Chinese. Methods We recorded 12- lead ECG （CardioSoft, v4.2） in 1365 residents in the Jingning County, Zhejiang Province, China. LVH was defined according to the gender-specific Sokolow-Lyon and Cornell product ECG criteria. Results Regardless of whether the Sokolow-Lyon or Cornell product ECG criteria was used, the prevalence of LVH （20.7% and 4.8%, respectively） significantly （P〈0.0001） increased with male gender （odds ratio [OR] 2.33 and 7.15） and systolic blood pressure （per 10 mm Hg increase, OR 1.46 and 1.33）. If the Sokolow-Lyon criteria was used, the prevalence of LVH was also influenced by alcohol intake （OR 1.44, P=-0.03） and body mass index （OR 0.83, P=0.0005）. The association between the Sokolow-Lyon voltage amplitude and the ACE I/D polymorphism was dependent on antihypertensive therapy （P=0.01）. In 1262 untreated subjects, but not 103 patients on antihypertensive medication, the ACE DD compared with II subjects had significantly higher Sokolow-Lyon voltage amplitudes （29.8：-0.6 vs. 28.0-3：0.5 mV, P=-0.02） and higher risk of LVH （OR 1.74, 95% CI： 1.12-2.69, P=-0.01）. Conclusion LVH is prevalent in Chinese, and is associated with systolic blood pressure and the ACE D allele. The genetic association might be modulated by antihypertensive therapy（J Geriatr Cardio12009; 6：131-136）.展开更多
Lepidium sativum（commonly known as garden cress） belongs to the family Brassicaceae. It is a fastgrowing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic,...Lepidium sativum（commonly known as garden cress） belongs to the family Brassicaceae. It is a fastgrowing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic,hypoglycemic, nephrocurative and nephroprotective activities. In the present study, we assessed the genetic diversity of various genotypes of L. sativum using inter-simple sequence repeat（ISSR） markers. Out of 41 ISSR primers screened, 32 primers showed significant, clear and reproducible bands. A total of 510 amplified bands were obtained using 32 ISSR primers, out of which 422 bands were polymorphic and 88 bands were monomorphic. The percentage of polymorphism was found to be 82. A total of 35 unique alleles ranging insize from 200 to 2,900 bp were observed.Cluster analysis based on unweighted pair-group method,arithmetic mean divided the 18 genotypes into two main clusters, with the first having only HCS-08 genotype of L.sativum and other having all of the other 17 genotypes. The Jaccard similarity coefficient revealed a broad range32–72 % genetic relatedness among the 18 genotypes.展开更多
基金supported by National Key Projects of China (No.2001BA302B)
文摘Two types of markers-random amplified polymorphic DNA （RAPD） and simple sequence repeat DNA （SSR）-have been used to characterize the genetic diversity among nine mutant lines of transgenic wheat intermediated by low energy ion beam and their four receptor cultivars. The objectives of this study were to analyze RAPD-based and SSR-based genetic variance among transgenic wheat lines and with their receptors, and to find specific genetic markers of special traits of transgenic wheat lines. 170 RAPD primers were amplified to 733 fragments in all the experimental materials. There were 121 polymorphic fragments out of the 733 fragments with a ratio of polymorphic fragments of 16.5%. 29 SSR primer pairs were amplified to 83 fragments in all the experiment materials. There were 57 polymorphic fragments out of the 83 fragments with a ratio of polymorphic fragments of 68.7%. The dendrograms were prepared based on a genetic distance matrix using the UPGMA （Unweighted Pair-group Method with Arithmetic averaging） algorithm, which corresponded well to the results of the wheat pedigree analysis and separated the 13 genotypes into four groups. Association analysis between RAPD and SSR markers with the special traits of transgenic wheat mutant lines discovered that three RAPD markers, sl, opt- 16, and f14, were significantly associated with the muticate trait, while three SSR markers, Rht8 （Xgwm261）, Rht-Blb, and Rht-Dlb, highly associated with the dwarf trait. These markers will be useful for marker-assistant breeding and can be used as candidate markers for further gene mapping and cloning.
基金The authors gratefully acknowledge the voluntary collaboration of the study participants and the support of the local public health authorities of Jingning County, Zhejiang Province, China. This study was financially supported by grants from the National Natural Science Foundation of China （grants 30871360 and 30871081）, Beij＇ing, China, and the Shanghai Commissions of Science and Technology （grant 07JC14047 and the ＂Rising Star＂ program 06QA14043） and Education （grant 07ZZ32 and the ＂Dawn＂ program 08SG20）, and the European Union （InGenious HyperCare LSHM-CT-2006-037093 and HYPERGENES FP7-HEALTH-2007-201550）. Dr Alexander Headley was supported by the AusAID Australian Youth Ambassadors for Development scheme and the George Foundation of the George Institute for International Health, Sydney, Australia.
文摘Objective There is little population-based data on the prevalence and the environmental or genetic determinants of left ventricular hypertrophy （LVH） in China. The purpose of this paper is to study LVH in relation to systolic blood pressure and the angiotensin converting enzyme （ACE） insertion/deletion（I/D） polymorphism in Chinese. Methods We recorded 12- lead ECG （CardioSoft, v4.2） in 1365 residents in the Jingning County, Zhejiang Province, China. LVH was defined according to the gender-specific Sokolow-Lyon and Cornell product ECG criteria. Results Regardless of whether the Sokolow-Lyon or Cornell product ECG criteria was used, the prevalence of LVH （20.7% and 4.8%, respectively） significantly （P〈0.0001） increased with male gender （odds ratio [OR] 2.33 and 7.15） and systolic blood pressure （per 10 mm Hg increase, OR 1.46 and 1.33）. If the Sokolow-Lyon criteria was used, the prevalence of LVH was also influenced by alcohol intake （OR 1.44, P=-0.03） and body mass index （OR 0.83, P=0.0005）. The association between the Sokolow-Lyon voltage amplitude and the ACE I/D polymorphism was dependent on antihypertensive therapy （P=0.01）. In 1262 untreated subjects, but not 103 patients on antihypertensive medication, the ACE DD compared with II subjects had significantly higher Sokolow-Lyon voltage amplitudes （29.8：-0.6 vs. 28.0-3：0.5 mV, P=-0.02） and higher risk of LVH （OR 1.74, 95% CI： 1.12-2.69, P=-0.01）. Conclusion LVH is prevalent in Chinese, and is associated with systolic blood pressure and the ACE D allele. The genetic association might be modulated by antihypertensive therapy（J Geriatr Cardio12009; 6：131-136）.
文摘Lepidium sativum（commonly known as garden cress） belongs to the family Brassicaceae. It is a fastgrowing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic,hypoglycemic, nephrocurative and nephroprotective activities. In the present study, we assessed the genetic diversity of various genotypes of L. sativum using inter-simple sequence repeat（ISSR） markers. Out of 41 ISSR primers screened, 32 primers showed significant, clear and reproducible bands. A total of 510 amplified bands were obtained using 32 ISSR primers, out of which 422 bands were polymorphic and 88 bands were monomorphic. The percentage of polymorphism was found to be 82. A total of 35 unique alleles ranging insize from 200 to 2,900 bp were observed.Cluster analysis based on unweighted pair-group method,arithmetic mean divided the 18 genotypes into two main clusters, with the first having only HCS-08 genotype of L.sativum and other having all of the other 17 genotypes. The Jaccard similarity coefficient revealed a broad range32–72 % genetic relatedness among the 18 genotypes.
