Fasciclin-like arabinogalactan proteins(FLAs),a subclass of arabinogalactan proteins(AGPs),are usually involved in cell development in plants.To investigate the expression profiling as well
Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls...Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls and which could play a role in controlling early fiber development.Chief among these was GhMYB25,a Mixta-like MYB gene.Transgenic GhMYB25-silenced cotton展开更多
Cotton(Gossypium hirsutum L.) is the leading fiber crop and one of the mainstays of the economy in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear
Tomato(Solanum lycopersicum L.)is a thermophilic vegetable crop,but sensitive to high temperature stress,especially under the greenhouse conditions.Due to global climate changes,heat stress has now become a great thre...Tomato(Solanum lycopersicum L.)is a thermophilic vegetable crop,but sensitive to high temperature stress,especially under the greenhouse conditions.Due to global climate changes,heat stress has now become a great threat to tomato production and fruit quality.Many studies have been conducted to determine the functions of genes in tomato responsive to abiotic and biotic stresses,but transcriptomic information on heat stress responses of tomato fruit is still limited.To investigate heat stress associated genes in tomato fruit,a cDNA library was constructed using fruit harvested from tomato cv.P19-9 plants grown under 42℃for 0,1,2 and4 h and the expression profiles of heat stress responsive genes in tomato fruit were analyzed through RNA-seq.A total of 632224558 clean high quality paired-end reads were obtained and then mapped to reference genome for RNA-seq analysis.After quality control analysis,alignment analysis and transcript assembly,a total of 55457 RNA transcripts were obtained with functional annotations.Overall,6869 differentially expressed genes(DEGs)were identified with a significant response to one or more of the three heat stress treatment times.Based on GO enrichment analysis,22 genes potentially involved in tomato thermo-tolerance were selected and validated for their expressions through qPCR.The expression profile of tomato fruit genes obtained in this study could shed light on the mechanism and gene editing breeding projects for tomato thermo-tolerance.These findings could also benefit improvement of harvest and storage of tomato in greenhouse.展开更多
文摘Fasciclin-like arabinogalactan proteins(FLAs),a subclass of arabinogalactan proteins(AGPs),are usually involved in cell development in plants.To investigate the expression profiling as well
文摘Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls and which could play a role in controlling early fiber development.Chief among these was GhMYB25,a Mixta-like MYB gene.Transgenic GhMYB25-silenced cotton
基金This work was supported by the National Natural Science Foundation of China (No 30370904and No 30671258)the National High Technology Research and Development Program(863 project)of China (No 2006AA10Z121)the Program for New Century Excellent Talents in University(No NCET-07-0712)
文摘Cotton(Gossypium hirsutum L.) is the leading fiber crop and one of the mainstays of the economy in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear
文摘Tomato(Solanum lycopersicum L.)is a thermophilic vegetable crop,but sensitive to high temperature stress,especially under the greenhouse conditions.Due to global climate changes,heat stress has now become a great threat to tomato production and fruit quality.Many studies have been conducted to determine the functions of genes in tomato responsive to abiotic and biotic stresses,but transcriptomic information on heat stress responses of tomato fruit is still limited.To investigate heat stress associated genes in tomato fruit,a cDNA library was constructed using fruit harvested from tomato cv.P19-9 plants grown under 42℃for 0,1,2 and4 h and the expression profiles of heat stress responsive genes in tomato fruit were analyzed through RNA-seq.A total of 632224558 clean high quality paired-end reads were obtained and then mapped to reference genome for RNA-seq analysis.After quality control analysis,alignment analysis and transcript assembly,a total of 55457 RNA transcripts were obtained with functional annotations.Overall,6869 differentially expressed genes(DEGs)were identified with a significant response to one or more of the three heat stress treatment times.Based on GO enrichment analysis,22 genes potentially involved in tomato thermo-tolerance were selected and validated for their expressions through qPCR.The expression profile of tomato fruit genes obtained in this study could shed light on the mechanism and gene editing breeding projects for tomato thermo-tolerance.These findings could also benefit improvement of harvest and storage of tomato in greenhouse.
