目的研究芍药苷-6-氧-苯磺酸酯(CP-25)通过抑制GRK2活性对骨关节炎(osteoarthritis,OA)小鼠膝关节软骨的保护作用。方法内侧半月板失稳(destabilization of the medial meniscus,DMM)手术诱导构建小鼠骨关节炎模型,实验分为假手术组、...目的研究芍药苷-6-氧-苯磺酸酯(CP-25)通过抑制GRK2活性对骨关节炎(osteoarthritis,OA)小鼠膝关节软骨的保护作用。方法内侧半月板失稳(destabilization of the medial meniscus,DMM)手术诱导构建小鼠骨关节炎模型,实验分为假手术组、模型组、CP-25给药组和帕罗西汀给药组。术后开始灌胃给药。给药12周处死动物,Micro-CT成像观察膝关节软骨退变、骨重塑异常等情况,番红固绿染色观察小鼠关节组织病理,免疫组化、免疫荧光检测软骨组织相关分子表达水平的影响。Western blot检测CP-25用药后软骨细胞的膜蛋白及总蛋白表达水平。结果模型小鼠关节软骨严重退变。CP-25可显著降低关节软骨骨赘数量及软骨下板厚度,促进软骨基质再生,减少软骨基质降解蛋白表达,对膝关节软骨有明显的保护作用。免疫组化和免疫荧光结果显示,CP-25治疗可显著降低膝关节组织中GRK2、ADAMTS5、MMP13的表达,并且升高膝关节组织中ColⅡ、Aggrecan表达。体外实验结果表明,CP-25给药可以显著降低GRK2的膜蛋白及总蛋白表达水平,升高EP4膜蛋白水平,降低MMP13水平。结论CP-25给药可显著促进OA小鼠关节软骨基质再生,减少软骨基质降解,对OA具有治疗作用,其机制与抑制GRK2介导的软骨基质代谢有关。展开更多
目的研究芍药苷-6′-O-苯磺酸酯(CP-25)在血管紧张素Ⅱ(AngⅡ)诱导的肾小球系膜细胞(MCs)增殖中的作用及相关机制。方法体外培养SV40 MES 13系膜细胞,以AngⅡ诱导MCs增殖,高内涵成像显微镜检测不同浓度CP-25(10、100、1000 nmol/L)对An...目的研究芍药苷-6′-O-苯磺酸酯(CP-25)在血管紧张素Ⅱ(AngⅡ)诱导的肾小球系膜细胞(MCs)增殖中的作用及相关机制。方法体外培养SV40 MES 13系膜细胞,以AngⅡ诱导MCs增殖,高内涵成像显微镜检测不同浓度CP-25(10、100、1000 nmol/L)对AngⅡ诱导的MCs增殖的影响;Western blot检测MCs中G蛋白偶联受体激酶-2(GRK2)和磷酸化p38(p-p38)蛋白表达水平;激光共聚焦显微镜检测GRK2和p-p38蛋白的荧光信号并分析二者共定位率;成像流式细胞仪检测MCs中GRK2入胞质的细胞比例。结果与对照组比较,AngⅡ可诱导MCs的增殖,增加GRK2和p-p38蛋白表达,上调GRK2和p-p38MAPK共定位率,提高GRK2入胞比例,差异有统计学意义(P<0.01)。CP-25(10、100、1000 nmol/L)可不同程度地抑制AngⅡ诱导的增殖,抑制GRK2、p-p38蛋白表达及GRK2和p-p38的共定位水平,降低GRK2入胞比例。结论CP-25可抑制AngⅡ诱导的MCs增殖,其机制与调节GRK2/p38信号有关。展开更多
OBJECTIVE To investigate the role and mechanism of G protein-coupled receptor kinase 2(GRK2)involving in hepatocel ular carcinoma(HCC)progression.METHODS Cel Counting Kit 8 and tumor colony formation assay were design...OBJECTIVE To investigate the role and mechanism of G protein-coupled receptor kinase 2(GRK2)involving in hepatocel ular carcinoma(HCC)progression.METHODS Cel Counting Kit 8 and tumor colony formation assay were designed to detect HCC cell proliferation,wound healing assay was to detect HCC migration.The correlation between GRK2 and early growth response-1(EGR1)were detected by RT-PCR and real-time PCR assays.Co-immunoprecipitation and Western blot assay were adopted to detect the relationship between GRK2and insulin-like growth factor 1 receptor(IGF-1R)signaling pathway.RESULTS In this study we find that GRK2plays an inhibition role in IGF1-induced HCC cell proliferation and migration.Overexpression of GRK2 causes a decrease in EGR1 expression,while knockdown of GRK2 leads to the dramatically increase in EGR1 expression in the treatment of IGF1.Through co-immunoprecipitation and Western blot assay,we confirm that GRK2can interact with IGF-1R and inhibiting IGF1-induced activation of IGF1R signaling pathway.Silencing EGR1attenuates GRK2 overexpression-caused inhibition of cell proliferation,tumor colony number and migrationactivity,while overexpressing of EGR1 restores the antiproliferative and migratory effect by GRK2 overexpression in HCCLM3 cells.CONCLUSION Taken together,these results suggest that GRK2 may inhibit IGF1-induced HCC cell growth and migration through down-regulation of EGR1 and indicate that enforced GRK2 may offer a potential therapeutic approach against HCC.展开更多
OBJECTIVE Increased efficacy without increased toxicity is expected when treating rheumatoid arthritis(RA).However,there are many difficulties associated with currently available RA treatments.Reportedly,CP-25,a new c...OBJECTIVE Increased efficacy without increased toxicity is expected when treating rheumatoid arthritis(RA).However,there are many difficulties associated with currently available RA treatments.Reportedly,CP-25,a new compound developed by our group,significantly inhibits the progression of arthritis in animal models through reducing membrane expression of GRK2.This study observed CP-25 monotherapy and combined administration with MTX/LEF in treating animal arthritis model and investigated possible mechanisms.METHODS We set up AA rat and collageninduced arthritis(CIA)mice model.Experimental groups were divided into normal group,vehicle group,monotherapy groups and CP-25-combined MTX/LEF groups.We focused on the role of GRK2 on macrophage polarization and fibro⁃blast-like synoviocytes(FLS)proliferation.We measured cytokine levels,phosphorylation and protein expression,and interactions between proteins.Targeted disruption of GRK2 in FLS and macrophages through GRK2 siRNA and CRIS⁃PR/Cas9.RESULTS Equivalent therapeutic effects were observed between CP-25-combination groups and high-dose MTX/LEF groups.In the vehicle group,GRK2 membrane expression increased leading to the decreased GRK2-p-ERK interactions in FLS(leading to the phosphorylation of ERK related with FLS over-proliferation),and increased GRK2-EP4 interaction in macrophage(leading to the abnormal PGE2-EP4-cAMP-CREB signal related with imbalance of macro⁃phage polarization).CONCLUSION CP-25 monotherapy and combined administration with MTX/LEF ameliorate the progression of animal arthritis model.CP-25 inhibited p-ERK by reducing the membrane expression of GRK2 in FLS from AA rats.CP-25 restored normal PGE2-EP4-cAMP-CREB signal through inhibiting GRK2 transferring to membrane.These results highlighted CP-25 a kind of potential for treating patients with RA.展开更多
建立了适合佐剂性关节炎大鼠脾脏组织G蛋白偶联受体激酶2(GRK2)免疫荧光测定法。考察了免疫荧光测定的4个关键条件:(1)渗透剂Triton X-100的影响;(2)不同抗原修复液:柠檬酸缓冲液和p H 9.0乙二胺四乙酸(EDTA)缓冲液的影响;(3)5%胎牛血...建立了适合佐剂性关节炎大鼠脾脏组织G蛋白偶联受体激酶2(GRK2)免疫荧光测定法。考察了免疫荧光测定的4个关键条件:(1)渗透剂Triton X-100的影响;(2)不同抗原修复液:柠檬酸缓冲液和p H 9.0乙二胺四乙酸(EDTA)缓冲液的影响;(3)5%胎牛血清封闭不同时间(20、30、40、50min)的影响;(4)不同一抗浓度(1∶50、1∶100、1∶200、1∶400、1∶500)的影响。实验结果显示,Triton X-100通透增强了抗原的表达;1∶100的一抗浓度对荧光信号强度最合适;封闭50 min能显著去除背景非特异性染色;柠檬酸缓冲液优于p H 9.0 EDTA缓冲液,提高了抗原表达。展开更多
文摘目的研究芍药苷-6-氧-苯磺酸酯(CP-25)通过抑制GRK2活性对骨关节炎(osteoarthritis,OA)小鼠膝关节软骨的保护作用。方法内侧半月板失稳(destabilization of the medial meniscus,DMM)手术诱导构建小鼠骨关节炎模型,实验分为假手术组、模型组、CP-25给药组和帕罗西汀给药组。术后开始灌胃给药。给药12周处死动物,Micro-CT成像观察膝关节软骨退变、骨重塑异常等情况,番红固绿染色观察小鼠关节组织病理,免疫组化、免疫荧光检测软骨组织相关分子表达水平的影响。Western blot检测CP-25用药后软骨细胞的膜蛋白及总蛋白表达水平。结果模型小鼠关节软骨严重退变。CP-25可显著降低关节软骨骨赘数量及软骨下板厚度,促进软骨基质再生,减少软骨基质降解蛋白表达,对膝关节软骨有明显的保护作用。免疫组化和免疫荧光结果显示,CP-25治疗可显著降低膝关节组织中GRK2、ADAMTS5、MMP13的表达,并且升高膝关节组织中ColⅡ、Aggrecan表达。体外实验结果表明,CP-25给药可以显著降低GRK2的膜蛋白及总蛋白表达水平,升高EP4膜蛋白水平,降低MMP13水平。结论CP-25给药可显著促进OA小鼠关节软骨基质再生,减少软骨基质降解,对OA具有治疗作用,其机制与抑制GRK2介导的软骨基质代谢有关。
基金The project supported by National Natural Science Foundation of China(81502123,81330081,81202596)Natural Science Foundation of Anhui Province(1308085QH130)+3 种基金Anhui Province Natural Science Foundation in University(KJ2014A119)Grants for Scientific Research of BSKY from Anhui Medical University(XJ201212)Specialized Research Fund for the Doctoral Program of Higher Education(20113420120006,20123420110003)Program for Tackling Key Problems in Science and Technology by Anhui Province(1301042098)
文摘OBJECTIVE To investigate the role and mechanism of G protein-coupled receptor kinase 2(GRK2)involving in hepatocel ular carcinoma(HCC)progression.METHODS Cel Counting Kit 8 and tumor colony formation assay were designed to detect HCC cell proliferation,wound healing assay was to detect HCC migration.The correlation between GRK2 and early growth response-1(EGR1)were detected by RT-PCR and real-time PCR assays.Co-immunoprecipitation and Western blot assay were adopted to detect the relationship between GRK2and insulin-like growth factor 1 receptor(IGF-1R)signaling pathway.RESULTS In this study we find that GRK2plays an inhibition role in IGF1-induced HCC cell proliferation and migration.Overexpression of GRK2 causes a decrease in EGR1 expression,while knockdown of GRK2 leads to the dramatically increase in EGR1 expression in the treatment of IGF1.Through co-immunoprecipitation and Western blot assay,we confirm that GRK2can interact with IGF-1R and inhibiting IGF1-induced activation of IGF1R signaling pathway.Silencing EGR1attenuates GRK2 overexpression-caused inhibition of cell proliferation,tumor colony number and migrationactivity,while overexpressing of EGR1 restores the antiproliferative and migratory effect by GRK2 overexpression in HCCLM3 cells.CONCLUSION Taken together,these results suggest that GRK2 may inhibit IGF1-induced HCC cell growth and migration through down-regulation of EGR1 and indicate that enforced GRK2 may offer a potential therapeutic approach against HCC.
基金National Natural Science Foundation of China(8133008181673444+3 种基金3120067581573443)Anhui Province Natural Science Fund(outstanding youth)(170808J10)Key Projects of Outstanding young Talent Support Program in Colleges and Universities(gxyq ZD2016043)
文摘OBJECTIVE Increased efficacy without increased toxicity is expected when treating rheumatoid arthritis(RA).However,there are many difficulties associated with currently available RA treatments.Reportedly,CP-25,a new compound developed by our group,significantly inhibits the progression of arthritis in animal models through reducing membrane expression of GRK2.This study observed CP-25 monotherapy and combined administration with MTX/LEF in treating animal arthritis model and investigated possible mechanisms.METHODS We set up AA rat and collageninduced arthritis(CIA)mice model.Experimental groups were divided into normal group,vehicle group,monotherapy groups and CP-25-combined MTX/LEF groups.We focused on the role of GRK2 on macrophage polarization and fibro⁃blast-like synoviocytes(FLS)proliferation.We measured cytokine levels,phosphorylation and protein expression,and interactions between proteins.Targeted disruption of GRK2 in FLS and macrophages through GRK2 siRNA and CRIS⁃PR/Cas9.RESULTS Equivalent therapeutic effects were observed between CP-25-combination groups and high-dose MTX/LEF groups.In the vehicle group,GRK2 membrane expression increased leading to the decreased GRK2-p-ERK interactions in FLS(leading to the phosphorylation of ERK related with FLS over-proliferation),and increased GRK2-EP4 interaction in macrophage(leading to the abnormal PGE2-EP4-cAMP-CREB signal related with imbalance of macro⁃phage polarization).CONCLUSION CP-25 monotherapy and combined administration with MTX/LEF ameliorate the progression of animal arthritis model.CP-25 inhibited p-ERK by reducing the membrane expression of GRK2 in FLS from AA rats.CP-25 restored normal PGE2-EP4-cAMP-CREB signal through inhibiting GRK2 transferring to membrane.These results highlighted CP-25 a kind of potential for treating patients with RA.
文摘建立了适合佐剂性关节炎大鼠脾脏组织G蛋白偶联受体激酶2(GRK2)免疫荧光测定法。考察了免疫荧光测定的4个关键条件:(1)渗透剂Triton X-100的影响;(2)不同抗原修复液:柠檬酸缓冲液和p H 9.0乙二胺四乙酸(EDTA)缓冲液的影响;(3)5%胎牛血清封闭不同时间(20、30、40、50min)的影响;(4)不同一抗浓度(1∶50、1∶100、1∶200、1∶400、1∶500)的影响。实验结果显示,Triton X-100通透增强了抗原的表达;1∶100的一抗浓度对荧光信号强度最合适;封闭50 min能显著去除背景非特异性染色;柠檬酸缓冲液优于p H 9.0 EDTA缓冲液,提高了抗原表达。
