Stepwise selection approach was adopted to obtain glyphosate-tolerant upland cotton mutant(R1098) from the embryogenic calli of Coker 312(Gossypium hirsutum L.).The calli were transferred to selection medium and multi...Stepwise selection approach was adopted to obtain glyphosate-tolerant upland cotton mutant(R1098) from the embryogenic calli of Coker 312(Gossypium hirsutum L.).The calli were transferred to selection medium and multi-step selection pressure process was carried out until the展开更多
A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows： the concentration of digoxin tag pr...A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows： the concentration of digoxin tag probe was 0.5 μmol · L^-1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.展开更多
Competitions of light,nutrient and water between transgenic glyphosate resistant soybean and weed were evaluated with different herbicides(glyphosate,acetochlor,and the mixture of fomesafen and quizalofop-p-ethyl)in f...Competitions of light,nutrient and water between transgenic glyphosate resistant soybean and weed were evaluated with different herbicides(glyphosate,acetochlor,and the mixture of fomesafen and quizalofop-p-ethyl)in filed,which displayed that the application of the mixture of fomesafen and quizalofop-p-ethyl on soybeans had the best anti-herbicidal effect while glyphosate had the weakest effect.The half-life extended by herbicide mixture would affect the soil nutrients.Weed-soybean competitions affected the absorption of nutrients on soybean and yield.However,effective control of weeds could significantly reduce the weed density,improve the field through different layers of light transmittance and the absorption of nutrients on soybean,and enhance the comprehensive competitiveness of soybean.展开更多
Thirty lines of bean were tested for resistance to glyphosate in the field.Obvious difference in resistance response to glyphosate was detected when glyphosate was applied at the rate of 1kg ai/ha on the 15th day afte...Thirty lines of bean were tested for resistance to glyphosate in the field.Obvious difference in resistance response to glyphosate was detected when glyphosate was applied at the rate of 1kg ai/ha on the 15th day after emergence of seedlings.The ling 89-05 showed high resistance with a survival of 77%.Even after glyphosate was reapplied,the survival rats was still 20%.Obvious segregation was found in Progency of 89-05 for resistance to glyphosate;the survival rate of lined 89-05-1 89-05-2 and 89-05-3 were,respectively.42.3%,51.4% and 85.7% at 1kg ai/ha of the herbicide.Progenies 89-05-3 continued to show resistance and lines 89-05-3-5 and 89-05-3-7 both had a survival rate of 100%.When glyphosate was applied with a rate over 1kg ai/ha, the survival rate of the 89-05 -3- 5 was still between 50%-98%.展开更多
文摘Stepwise selection approach was adopted to obtain glyphosate-tolerant upland cotton mutant(R1098) from the embryogenic calli of Coker 312(Gossypium hirsutum L.).The calli were transferred to selection medium and multi-step selection pressure process was carried out until the
文摘A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows： the concentration of digoxin tag probe was 0.5 μmol · L^-1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.
基金Supported by Environmental Safety Assessment of Key Research Projects of Ministry of Agriculture(2016ZX08011-003)
文摘Competitions of light,nutrient and water between transgenic glyphosate resistant soybean and weed were evaluated with different herbicides(glyphosate,acetochlor,and the mixture of fomesafen and quizalofop-p-ethyl)in filed,which displayed that the application of the mixture of fomesafen and quizalofop-p-ethyl on soybeans had the best anti-herbicidal effect while glyphosate had the weakest effect.The half-life extended by herbicide mixture would affect the soil nutrients.Weed-soybean competitions affected the absorption of nutrients on soybean and yield.However,effective control of weeds could significantly reduce the weed density,improve the field through different layers of light transmittance and the absorption of nutrients on soybean,and enhance the comprehensive competitiveness of soybean.
文摘Thirty lines of bean were tested for resistance to glyphosate in the field.Obvious difference in resistance response to glyphosate was detected when glyphosate was applied at the rate of 1kg ai/ha on the 15th day after emergence of seedlings.The ling 89-05 showed high resistance with a survival of 77%.Even after glyphosate was reapplied,the survival rats was still 20%.Obvious segregation was found in Progency of 89-05 for resistance to glyphosate;the survival rate of lined 89-05-1 89-05-2 and 89-05-3 were,respectively.42.3%,51.4% and 85.7% at 1kg ai/ha of the herbicide.Progenies 89-05-3 continued to show resistance and lines 89-05-3-5 and 89-05-3-7 both had a survival rate of 100%.When glyphosate was applied with a rate over 1kg ai/ha, the survival rate of the 89-05 -3- 5 was still between 50%-98%.
