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胎儿弯杆菌病TaqMan实时荧光定量PCR检测方法的建立 被引量:3
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作者 赵海玲 刘慧芳 +3 位作者 杜艳芬 司微 王春来 刘思国 《中国预防兽医学报》 CAS CSCD 北大核心 2009年第11期874-877,共4页
为建立胎儿弯杆菌(C.fetus)定量检测方法,本研究根据C.fetus毒力因子表面蛋白(SapA)的基因序列设计引物和一条特异的TaqMan水解探针,建立了一种敏感、特异、重复性好的快速检测C.fetus的TaqMan荧光定量PCR方法。对该方法的特异性与敏感... 为建立胎儿弯杆菌(C.fetus)定量检测方法,本研究根据C.fetus毒力因子表面蛋白(SapA)的基因序列设计引物和一条特异的TaqMan水解探针,建立了一种敏感、特异、重复性好的快速检测C.fetus的TaqMan荧光定量PCR方法。对该方法的特异性与敏感性研究,结果显示,该方法检测C.fetus结果均为阳性,而非C.fetus均为阴性;对带有SapA基因的阳性质粒的检测敏感性为108拷贝~102拷贝/μL范围内具有良好的线性关系,可敏感地检测到模板中13个拷贝的细菌DNA,其灵敏度是常规PCR方法的100倍。该方法具有简便、快速、特异性强、敏感性高等特点。该方法为C.fetus快速检测试剂盒的研制打下了良好的基础。 展开更多
关键词 C.fetus 表面蛋白基因SapA 实时荧光PCR TaqMan水解探针
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Study on features of female fetal reproductive system development in Chinese northeast fine-wool sheep
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作者 LIU Yunbo ZHANG Guixue +3 位作者 HU Pengfei HUANG He ZHENG Peng LI Wanhua 《Journal of Northeast Agricultural University(English Edition)》 CAS 2007年第4期318-322,共5页
Some charaterasics of female fetal reproductive system were studied in different gestation phases of Chinese northeast fine-wool sheep. The results showed that reproductive system consisted of ovary, oviduct, uterus a... Some charaterasics of female fetal reproductive system were studied in different gestation phases of Chinese northeast fine-wool sheep. The results showed that reproductive system consisted of ovary, oviduct, uterus and genitalia. The fetal ovaries were granular in early phase and became elliptic in later phase. The functional formula of ovarian weight, length and volume was obtained. Primary oocyte was encapsulated by monolayer cells in 7-week fetal ovary cortex. Primordial follicles were formed in 8-week fetal ovary, the follicular cells were sporadically arranged and not always regular. Complete organization of primordial follicles appeared until 10-week gestation, most of them scattered in nests in the following weeks of gestation. Two types of follicular complexes were found in fetal. There were continuous and quick mitosis of oogonia in fetal ovaries, large complexes were formed by oogoniaes encapsulated by multilayer of follicular cells, then they differentiated into small complexes, one or more primary oocytes were formed by mitosis of oogonia, primordial follicles which were caused by follicular cell approaching into them developed. 展开更多
关键词 sheep FETUS OVARY OOGONIA OOCYTE follicle
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参考文献著录说明及格式示例
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《中国乳业》 2023年第6期35-35,共1页
[期刊][1]张漫,顾宪红,陈小秋,等.我国鲁药行业现状概述[J].中国猪业,2012,7(5):57-59.[2]Tremblay GF,Mate J,Dufour J J,et al.Survival rate and development of fetuses during the first 30 daysof gestation after folic acid add... [期刊][1]张漫,顾宪红,陈小秋,等.我国鲁药行业现状概述[J].中国猪业,2012,7(5):57-59.[2]Tremblay GF,Mate J,Dufour J J,et al.Survival rate and development of fetuses during the first 30 daysof gestation after folic acid addition to a swine diet[J].Journal of Animal Science,1989,67(3):724732. 展开更多
关键词 参考文献著录 SURVIVAL FETUS
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参考文献著录说明及格式示例
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《中国乳业》 2024年第12期81-81,共1页
[期刊][1]张漫,顾宪红.陈小秋,等我国普药行业现状概述[J].中国猪业,2012.7(5):57-9.[2]Tembly G F.Mare J J.Dofour J J,et al.Srvival rale and development of fetuses during the first 30 days of gestation after folic acid addi... [期刊][1]张漫,顾宪红.陈小秋,等我国普药行业现状概述[J].中国猪业,2012.7(5):57-9.[2]Tembly G F.Mare J J.Dofour J J,et al.Srvival rale and development of fetuses during the first 30 days of gestation after folic acid addition to a swine diet[J].Journal of Animal Science,1989,67(3):724-732. 展开更多
关键词 参考文献著录 SWINE FETUS
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