The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in f...The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in farm manure treatment were both higher than that of other treatments. The intensity of denitrification in chemical treatment was higher than that of manure treatment. The content of organic matter in soil was correlated with the intensity of nitrification and denitrification, and the coefficients were respectively 0.9981 and 0.8693.展开更多
The analyses on the mathematical models of the long- term location test in black soil in Song-nen Plain shows that farm manure and chemical fertilizers make great contribution to rapidly available-N in soil, while sta...The analyses on the mathematical models of the long- term location test in black soil in Song-nen Plain shows that farm manure and chemical fertilizers make great contribution to rapidly available-N in soil, while stalk makes less contribution. The most optimal pattern of fertilizing materials is 28 t / ha of farm manure, 630 kg / ha chemical fertilizers and 2.41 / ha of stalk.展开更多
【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference fo...【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor.展开更多
The objective of this incubation study was to comprehensively evaluate the effects of organic fertilizer and biochar,both individually and in combination,on soil respiration dynamics,key enzyme activities and the conc...The objective of this incubation study was to comprehensively evaluate the effects of organic fertilizer and biochar,both individually and in combination,on soil respiration dynamics,key enzyme activities and the concentrations of various organic carbon components in degraded mollisol.This study used a completely randomized factorial design with three application rates of organic fertilizer(M0:0,M1:13.64 g·kg^(-1),M2:27.27 g·kg^(-1))and biochar(C0:0,C1:1.36 g·kg^(-1),C2:2.73 g·kg^(-1)),resulting in nine treatments with four replicates each.Soil CO_(2) flux,organic carbon fractions including the total,active and microbial biomass carbon,and enzyme activities(sucrase,catalase and polyphenol oxidase)were measured over 120 days.Results indicated that the factors and their interactions significantly influenced all measured parameters.Soil respiration peaked at day 15,with M1C2 treatment exhibiting a rate of 10.90-fold higher than the control.The concentrations of the total,active and microbial biomass organic carbon reached maximum values on days 60,30 and 80,respectively.Notably,M2C1 treatment yielded the highest total organic carbon(86.35%increase)and microbial biomass carbon(17.84%increase).Enzyme activities were significantly enhanced,with the highest catalase activity observed for M0C2 treatment(10.14%increase).These results demonstrated that the co-application of organic fertilizer and biochar was a effective strategy for improving soil biological health and promoting carbon sequestration in degraded mollisol.The treatment combining a high rate of organic fertilizer with a low rate of biochar(M2C1)was identified as the most promising amendment strategy for fertility restoration.展开更多
植物雄性不育（male sterility,MS）既是植物生殖生物学研究中的一个重要植物学性状,也是研究作物杂种优势利用的一个重要农艺性状,在作物育种和分子生物学研究中具有重要地位。植物雄性不育可分为细胞核雄性不育（genic male sterility...植物雄性不育（male sterility,MS）既是植物生殖生物学研究中的一个重要植物学性状,也是研究作物杂种优势利用的一个重要农艺性状,在作物育种和分子生物学研究中具有重要地位。植物雄性不育可分为细胞核雄性不育（genic male sterility,GMS）和细胞质雄性不育（cytoplasmic male sterility,CMS）。GMS由核不育基因控制,无正反交遗传效应;CMS由线粒体不育基因和核恢复基因（restorer of fertility,Rf）共同调控，线粒体基因导致不育，Rf能改变线粒体中不育基因的表达，使不育系恢复育性。展开更多
Using soil data of the Second National Field Survey,the soil fertility of wetland ecosystem of Dongting Lake was evaluated by using the technology of GIS and method of fuzzy evaluation.Integrated with the wetland actu...Using soil data of the Second National Field Survey,the soil fertility of wetland ecosystem of Dongting Lake was evaluated by using the technology of GIS and method of fuzzy evaluation.Integrated with the wetland actuality of Dongting Lake and particularity of paddy,seven factors (including soil organic matter,total nitrogen,total phosphorus,total potassium,available phosphorus,available potassium,and pH value),closely related with soil fertility,were chosen to establish the index system of synthetical evaluation.Based on the effect degree of each selected index on soil fertility,a judgment matrix was built,and the weight coefficient was determined by the method of correlation coefficient.Finally,under the support of the spatial analysis module of GIS (Geographic Information System),the spatial distribution properties of soil fertility in wetland ecosystem of Dongting Lake were studied.The results show that the soil fertility of Dongting Lake wetland ecosystem is not very good,and the area of type III and type IV achieves 69.8%.As a result,many countermeasures should be taken to improve the soil fertility.As for the spatial properties,the soil fertility level of central and west Dongting Lake is much higher than that of north and south part.The soil fertility of paddy field surpasses that of red soil,and the contents of soil organic matter and total nitrogen in paddy field are large.展开更多
By two years （2007-2008） located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, ...By two years （2007-2008） located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, Heilongjiang Province. The results showed that the combined application of organic and inorganic fertilizers could increase the organinc matter, alkaline nitrogen, available phosphorus and available potassium. At the same time, the increasing application of organic fertilizer could reduce the soil bulk density and improve the field moisture capacity. Field moisture capacity and organic matter of the combined application of high quantities of organic manure and inorganic fertilizers AtB5 treatment increased the fhstest, organic matter increased by 3.33 g. kg and field moisture capacity increased by 11.25% than the beginning of the experiment. Under the same fertilization, the combined application of organic and inorganic fertilizers＇ increasing production range was higher than the single chemical fertilizers＇ which was from 0.8% to 9.4%. The results showed that the combined application of organic and inorganic fertilizers could increase the nutrient contents of soil and also was the highest productivity contribution to black soil fertility. It was the best fertilization structure of increasing productivity level and improving the soil fertility.展开更多
How to improve the water use efficiency of rice in black soil regions was studied. The black soil region in paddy fields was chosen as the research object. The research showed the fertilizer coupling mathematical mode...How to improve the water use efficiency of rice in black soil regions was studied. The black soil region in paddy fields was chosen as the research object. The research showed the fertilizer coupling mathematical model with N,P,K,irrigation water( W) and water use efficiency( WUE),which was set up under the condition of controlled irrigation with quadratic D- 416 optimized saturation design. The results show that the decending order of single factor' s influence on the WUE was N,K,P and W. All the interactions between N&P,N&K,N&W,K&P,P&W and K&W on the WUE were raised initially,and when reached a certain value,they began to decline. The decending order of each interaction on the WUE was K&P,K&W,N&K,N&P,P&W and N&W. When the WUE was targeted within 1. 8- 2. 5 kg / km^3,an optimized proportion plan was obtained in the 95% confidence interval,i. e. N 87. 76- 103. 32 kg / hm^2,K_2 O 52. 37- 66. 53 kg / hm^2 and P_2O_536. 80- 46. 71 kg / hm^2. Furthermore,the late tillering of the soil moisture content was 70. 07%- 72. 57% of the saturated moisture content.展开更多
The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in He...The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in Heilongjiang Province. The application of soil phosphorus activator was able to increase the quantity of bacteria and fungi in soil, but its effect on actinomycetes in soil was not significant. The application of microbial inoculants increased the urease and sucrase activities in soil over the growing season, but only at the maturing stage soil acid phosphatase activity was enhanced with the applying soil phosphorus activator. The application of soil phosphorus activator increased alkali-hydrolyzable nitrogen and available phosphorus contents in soil, but did not increase available potassium content in soil. The optimal microbial inoculant application rate as applied as soil phosphorus activator was 7.5 kg hm-2.展开更多
文摘The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in farm manure treatment were both higher than that of other treatments. The intensity of denitrification in chemical treatment was higher than that of manure treatment. The content of organic matter in soil was correlated with the intensity of nitrification and denitrification, and the coefficients were respectively 0.9981 and 0.8693.
文摘The analyses on the mathematical models of the long- term location test in black soil in Song-nen Plain shows that farm manure and chemical fertilizers make great contribution to rapidly available-N in soil, while stalk makes less contribution. The most optimal pattern of fertilizing materials is 28 t / ha of farm manure, 630 kg / ha chemical fertilizers and 2.41 / ha of stalk.
基金Key Research project of Gansu Province of China(22YF7NA108)National Natural Science Foundation of China(31860549)+1 种基金Industry Supporting Project from Education Department of Gansu Province(2023CYZC-49)Major Science and Technology project of Gansu province(24ZDNA006)。
文摘【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor.
基金Supported by the Special Fund for Agro-scientific Research in Public Interest in China(201503119-06-01)。
文摘The objective of this incubation study was to comprehensively evaluate the effects of organic fertilizer and biochar,both individually and in combination,on soil respiration dynamics,key enzyme activities and the concentrations of various organic carbon components in degraded mollisol.This study used a completely randomized factorial design with three application rates of organic fertilizer(M0:0,M1:13.64 g·kg^(-1),M2:27.27 g·kg^(-1))and biochar(C0:0,C1:1.36 g·kg^(-1),C2:2.73 g·kg^(-1)),resulting in nine treatments with four replicates each.Soil CO_(2) flux,organic carbon fractions including the total,active and microbial biomass carbon,and enzyme activities(sucrase,catalase and polyphenol oxidase)were measured over 120 days.Results indicated that the factors and their interactions significantly influenced all measured parameters.Soil respiration peaked at day 15,with M1C2 treatment exhibiting a rate of 10.90-fold higher than the control.The concentrations of the total,active and microbial biomass organic carbon reached maximum values on days 60,30 and 80,respectively.Notably,M2C1 treatment yielded the highest total organic carbon(86.35%increase)and microbial biomass carbon(17.84%increase).Enzyme activities were significantly enhanced,with the highest catalase activity observed for M0C2 treatment(10.14%increase).These results demonstrated that the co-application of organic fertilizer and biochar was a effective strategy for improving soil biological health and promoting carbon sequestration in degraded mollisol.The treatment combining a high rate of organic fertilizer with a low rate of biochar(M2C1)was identified as the most promising amendment strategy for fertility restoration.
文摘植物雄性不育（male sterility,MS）既是植物生殖生物学研究中的一个重要植物学性状,也是研究作物杂种优势利用的一个重要农艺性状,在作物育种和分子生物学研究中具有重要地位。植物雄性不育可分为细胞核雄性不育（genic male sterility,GMS）和细胞质雄性不育（cytoplasmic male sterility,CMS）。GMS由核不育基因控制,无正反交遗传效应;CMS由线粒体不育基因和核恢复基因（restorer of fertility,Rf）共同调控，线粒体基因导致不育，Rf能改变线粒体中不育基因的表达，使不育系恢复育性。
基金Projects(40971170,51039001) supported by the National Natural Science Foundation of ChinaProject(2007AA10Z222) supported by the National High Technology Research and Development Program of China
文摘Using soil data of the Second National Field Survey,the soil fertility of wetland ecosystem of Dongting Lake was evaluated by using the technology of GIS and method of fuzzy evaluation.Integrated with the wetland actuality of Dongting Lake and particularity of paddy,seven factors (including soil organic matter,total nitrogen,total phosphorus,total potassium,available phosphorus,available potassium,and pH value),closely related with soil fertility,were chosen to establish the index system of synthetical evaluation.Based on the effect degree of each selected index on soil fertility,a judgment matrix was built,and the weight coefficient was determined by the method of correlation coefficient.Finally,under the support of the spatial analysis module of GIS (Geographic Information System),the spatial distribution properties of soil fertility in wetland ecosystem of Dongting Lake were studied.The results show that the soil fertility of Dongting Lake wetland ecosystem is not very good,and the area of type III and type IV achieves 69.8%.As a result,many countermeasures should be taken to improve the soil fertility.As for the spatial properties,the soil fertility level of central and west Dongting Lake is much higher than that of north and south part.The soil fertility of paddy field surpasses that of red soil,and the contents of soil organic matter and total nitrogen in paddy field are large.
基金Supported by Black Soil Conservation Tillage and Oriented Cultivation Technology Research (GB06B107-1)Innovation Fund of Northeast Agricultural University (CXP7003-3-3)+1 种基金Northeast Agricultural University and the Scientific Research Fund of Heilongjiang Province to Black CollegesUniversities Cold Key Laboratory of Utilization and Protection of Open-funded Projects (GXS08-5)
文摘By two years （2007-2008） located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, Heilongjiang Province. The results showed that the combined application of organic and inorganic fertilizers could increase the organinc matter, alkaline nitrogen, available phosphorus and available potassium. At the same time, the increasing application of organic fertilizer could reduce the soil bulk density and improve the field moisture capacity. Field moisture capacity and organic matter of the combined application of high quantities of organic manure and inorganic fertilizers AtB5 treatment increased the fhstest, organic matter increased by 3.33 g. kg and field moisture capacity increased by 11.25% than the beginning of the experiment. Under the same fertilization, the combined application of organic and inorganic fertilizers＇ increasing production range was higher than the single chemical fertilizers＇ which was from 0.8% to 9.4%. The results showed that the combined application of organic and inorganic fertilizers could increase the nutrient contents of soil and also was the highest productivity contribution to black soil fertility. It was the best fertilization structure of increasing productivity level and improving the soil fertility.
文摘How to improve the water use efficiency of rice in black soil regions was studied. The black soil region in paddy fields was chosen as the research object. The research showed the fertilizer coupling mathematical model with N,P,K,irrigation water( W) and water use efficiency( WUE),which was set up under the condition of controlled irrigation with quadratic D- 416 optimized saturation design. The results show that the decending order of single factor' s influence on the WUE was N,K,P and W. All the interactions between N&P,N&K,N&W,K&P,P&W and K&W on the WUE were raised initially,and when reached a certain value,they began to decline. The decending order of each interaction on the WUE was K&P,K&W,N&K,N&P,P&W and N&W. When the WUE was targeted within 1. 8- 2. 5 kg / km^3,an optimized proportion plan was obtained in the 95% confidence interval,i. e. N 87. 76- 103. 32 kg / hm^2,K_2 O 52. 37- 66. 53 kg / hm^2 and P_2O_536. 80- 46. 71 kg / hm^2. Furthermore,the late tillering of the soil moisture content was 70. 07%- 72. 57% of the saturated moisture content.
文摘The experiment was conducted to study the impact of application of microbial inoculants, compared with no microbial fertilizer, on enzyme activity, microbial biomass and available nutrient contents in paddy soil in Heilongjiang Province. The application of soil phosphorus activator was able to increase the quantity of bacteria and fungi in soil, but its effect on actinomycetes in soil was not significant. The application of microbial inoculants increased the urease and sucrase activities in soil over the growing season, but only at the maturing stage soil acid phosphatase activity was enhanced with the applying soil phosphorus activator. The application of soil phosphorus activator increased alkali-hydrolyzable nitrogen and available phosphorus contents in soil, but did not increase available potassium content in soil. The optimal microbial inoculant application rate as applied as soil phosphorus activator was 7.5 kg hm-2.
