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Enterococcus faecalis RQ15产低温中性蛋白酶的纯化及酶学性质 被引量:3
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作者 朱巍巍 李杨 +3 位作者 朱万琴 王艳华 冀宝赢 孙翠焕 《食品科学》 EI CAS CSCD 北大核心 2011年第11期239-242,共4页
采用DEAE Sepharose Fast Flow和SuperdexTM 75对Enterococcus faecalis RQ15产蛋白酶进行纯化和酶学性质研究。SDS-PAGE测定该蛋白酶分子质量为32.4kD,最适作用温度35~40℃,最适pH7.5。20~40℃之间酶活较高,pH值耐受范围广泛,具有低... 采用DEAE Sepharose Fast Flow和SuperdexTM 75对Enterococcus faecalis RQ15产蛋白酶进行纯化和酶学性质研究。SDS-PAGE测定该蛋白酶分子质量为32.4kD,最适作用温度35~40℃,最适pH7.5。20~40℃之间酶活较高,pH值耐受范围广泛,具有低温蛋白酶的特征。Zn2+对蛋白酶有激活作用,Ag+、Hg2+和EDTA-Na2对酶有显著抑制作用。纯酶最适作用条件下对酪蛋白底物的Km和Vmax分别为1.31×10-4mol/L和6.92×10-6mol/(L.s)。 展开更多
关键词 enterococcusfaecalis 低温蛋白酶 纯化
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UV-TiO_2 photocatalytic disinfection and photoreactivation of pathogenic bacterium in municipal wastewater
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作者 王西峰 胡晓莲 +1 位作者 龚昕 班云霄 《Journal of Central South University》 SCIE EI CAS CSCD 2016年第12期3115-3121,共7页
The disinfected bacteria will be a photoreactivation under the irradiation of the sunlight,and the light intensity plays an important role in the bacteria resurrection.The effect of light intensity on photoreactivatio... The disinfected bacteria will be a photoreactivation under the irradiation of the sunlight,and the light intensity plays an important role in the bacteria resurrection.The effect of light intensity on photoreactivation of Escherichia coli(E.coli) and Enterococcus faecalis(E.faecalis) in secondary effluents which were disinfected respectively by pure UV and UV-TiO_2 was investigated.The results show that the disinfection efficiency of UV-TiO_2 is much higher than that of the pure UV disinfection.The photoreactivation rate of E.coli is much higher in pure UV disinfection than in UV-TiO_2 photocatalytic disinfection.Under high light intensity in UV-TiO_2 disinfection,high resurrection rate can be induced.However,a higher resurrection rate can be introduced even under low light intensity in pure UV disinfection alone.Meanwhile,UV-TiO_2 disinfection has a strong inhibition effect on E.faecalis photoreactivation.When the light intensity is lower than 21 μW/cm^2,nearly no resurrection of E.faecalis occurs after 72 h resurrection irradiation,and a little resurrection rate is observed only under a strong photoreactivating light intensity. 展开更多
关键词 pathogenic bacteria inactivate UV-TiO2 disinfection PHOTOREACTIVATION Escherichia coli (E. coli) enterococcusfaecalis (E. facialis)
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