For space-borne gravitational wave detection missions based on the heterodyne interferometry principle,tilt-to-length(TTL)coupling noise is an important optical noise source,significantly influencing the accuracy of t...For space-borne gravitational wave detection missions based on the heterodyne interferometry principle,tilt-to-length(TTL)coupling noise is an important optical noise source,significantly influencing the accuracy of the measurement system.We present a method for analyzing TTL coupling noise under the joint influence of multiple factors.An equivalent simulated optical bench for the test mass interferometer was designed,and Gaussian beam tracing was adopted to simulate beam propagation.By simulating the interference signal,it can analyze the impact of various factors on the TTL coupling noise,including positional,beam parameters,detector parameters,and signal definition factors.On this basis,a random parameter space composed of multiple influential factors was constructed within a range satisfying the analysis requirement,and the corresponding simulation results from random sampling were evaluated via variance-based global sensitivity analysis.The calculated results of the main and total effect indexes show that the test mass rotation angle and the piston effect(lateral)significantly influence the TTL coupling noise in the test mass interferometer.The analysis provides a qualitative reference for designing and optimizing space-borne laser interferometry systems.展开更多
During the propagation of high-power lasers within internal channels,the laser beam heats the propagation medium,causing the thermal blooming effect that degrades the beam quality at the output.The intricate configura...During the propagation of high-power lasers within internal channels,the laser beam heats the propagation medium,causing the thermal blooming effect that degrades the beam quality at the output.The intricate configuration of the optical path within the internal channel necessitates complex and time-consuming efforts to assess the impact of thermal blooming effect on the optical path.To meet the engineering need for rapid evaluation of thermal blooming effect in optical paths,this study proposed a rapid simulation method for the thermal blooming effect in internal optical paths based on the finite element method.This method discretized the fluid region into infinitesimal elements and employed finite element method for flow field analysis.A simplified analytical model of the flow field region in complex internal channels was established,and regions with similar thermal blooming effect were divided within this model.Based on the calculated optical path differences within these regions,numerical simulations of phase distortion caused by thermal blooming were conducted.The calculated result were compared with those obtained using the existing methods.The findings reveal that for complex optical paths,the discrepancy between the two approaches is less than 3.6%,with similar phase distortion patterns observed.For L-type units,this method and the existing methods identify the same primary factors influencing aberrations and exhibit consistent trends in their variation.This method was used to analyze the impact of thermal blooming effect in a straight channel under different gravity directions.The results show that phase distortion varies with changes in the direction of gravity,and the magnitude of the phase difference is strongly correlated with the component of gravity perpendicular to the optical axis.Compared to the existing methods,this approach offers greater flexibility,obviates the need for complex custom analysis programming.The analytical results of this method enable a rapid assessment of the thermal blooming effect in optical paths within the internal channel.This is especially useful during the engineering design.These results also provide crucial references for developing strategies to suppress thermal blooming effect.展开更多
Abstract： A joint solution model of variabk：-mass flow in two-phase region and fluid-solid coupling heat transfer, concerned about the charge process of variable-mass thermodynamic system, is built up and calculated...Abstract： A joint solution model of variabk：-mass flow in two-phase region and fluid-solid coupling heat transfer, concerned about the charge process of variable-mass thermodynamic system, is built up and calculated by the finite element method （FEM）. The results are basically consistent with relative experimental data. The calculated average heat transfer coefficient reaches 1.7~105 W/（m2. K）. When the equal percentage valve is used, the system needs the minimum requirements of valve control, but brings the highest construction cost. With the： decrease of initial steam pressure, the heat transfer intensity also weakens but the steam flow increases. With the initial water filling coefficient increasing or the temperature of steam supply decreasing, the amount of accumulative steam flow increases with the growth of steam pressure. When the pressure of steam supply drops, the steam flow gradient increases during the maximum opening period of control valve, and causes the maximum steam flow to increase.展开更多
The pressure solution model of granular aggregates was introduced into a FEM code which was developed for the analysis of thermo-hydro-mechanical(T-H-M) coupling in porous medium. Aimed at creating a hypothetical mode...The pressure solution model of granular aggregates was introduced into a FEM code which was developed for the analysis of thermo-hydro-mechanical(T-H-M) coupling in porous medium. Aimed at creating a hypothetical model of nuclear waste disposal in unsaturated quartz aggregate rock mass with laboratory scale, two 4-year computation cases were designed: 1) The porosity and permeability of rock mass are functions of the pressure solution; 2) The porosity and the permeability are constants. Calculation results show that the magnitude and distribution of stresses in the rock mass of these two calculation cases are roughly the same. And, the porosity and the permeability decrease to 43%-54% and 4.4%-9.1% of their original values after case 1 being accomplished; but the negative pore water pressures in cases 1 and 2 are respectively 1.0-1.25 and 1.0-1.1 times of their initial values under the action of nuclear waste. Case 1 exhibits the obvious effect of pressure solution.展开更多
To predict three-dimensional temperature distribution of molten aluminum and its influencing factors inside an industrial aluminum holding furnace,a fluid-solid coupled method was presented.The fluid-solid coupled mat...To predict three-dimensional temperature distribution of molten aluminum and its influencing factors inside an industrial aluminum holding furnace,a fluid-solid coupled method was presented.The fluid-solid coupled mathematics models of aluminum holding furnace in the premixed combustion processing were established based on mass conservation,moment conservation,momentum conservation,energy conservation and chemistry species conservation.Computational results agree well with the test data of the typical condition.The maximum combustion temperature is 1 850 K.The average temperature of the molten aluminum is 1 158 K,and the maximum temperature difference is about 240 K.The average temperature increases 0.3 ℃ while the temperature of combustion air increases 1 ℃.The optimal excess air ratio is 1.25-1.30.展开更多
In actual production,deep coal mine roadways are often under typical static-dynamic coupling stress(SDCS)conditions with high ground stress and strong dynamic disturbances.With the increasing number of disasters and a...In actual production,deep coal mine roadways are often under typical static-dynamic coupling stress(SDCS)conditions with high ground stress and strong dynamic disturbances.With the increasing number of disasters and accidents induced by SDCS conditions,the safe and efficient production of coal mines is seriously threatened.Therefore,it is of great practical significance to study the deformation and failure characteristics of the roadway surrounding rock under SDCS.In this paper,the effects of different in-situ stress fields and dynamic load conditions on the surrounding rock are studied by numerical simulations,and the deformation and failure characteristics are obtained.According to the simulation results,the horizontal stress,vertical stress and dynamic disturbance have a positive correlation with the plastic failure of the surrounding rock.Among these factors,the influence of the dynamic disturbance is the most substantial.Under the same stress conditions,the extents of deformation and plastic failure of the roof and ribs are always greater than those of the floor.The effect of horizontal stresses on the roadway deformation is more notable than that of vertical stresses.The results indicate that for the roadway under high-stress conditions,the in-situ stress test must be strengthened first.After determining the magnitude of the in-situ stress,the location of the roadway should be reasonably arranged in the design to optimize the mining sequence.For roadways that are strongly disturbed by dynamic loads,rock supports(rebar/cable bolts,steel set etc.)that are capable of maintaining their effectiveness without failure after certain dynamic loads are required.The results of this study contribute to understanding the characteristics of the roadway deformation and failure under SDCS,and can be used to provide a basis for the support design and optimization under similar geological and geotechnical circumstances.展开更多
A complete geometric nonlinear formulation for rigid-flexible coupling dynamics of a flexible beam undergoing large overall motion was proposed based on virtual work principle, in which all the high-order terms relate...A complete geometric nonlinear formulation for rigid-flexible coupling dynamics of a flexible beam undergoing large overall motion was proposed based on virtual work principle, in which all the high-order terms related to coupling deformation were included in dynamic equations. Simulation examples of the flexible beam with prescribed rotation and free rotation were investigated. Numerical results show that the use of the first-order approximation coupling (FOAC) model may lead to a significant error when the flexible beam experiences large deformation or large deformation velocity. However, the correct solutions can always be obtained by using the present complete model. The difference in essence between this model and the FOAC model is revealed. These coupling high-order terms, which are ignored in FOAC model, have a remarkable effect on the dynamic behavior of the flexible body. Therefore, these terms should be included for the rigid-flexible dynamic modeling and analysis of flexible body undergoing motions with high speed.展开更多
When underground cavities are subjected to explosive stress waves,a uniquely damaged zone may appear due to the combined effect of dynamic loading and static pre-load stress.In this study,a rate-dependent two-dimensio...When underground cavities are subjected to explosive stress waves,a uniquely damaged zone may appear due to the combined effect of dynamic loading and static pre-load stress.In this study,a rate-dependent two-dimensional rock dynamic constitutive model was established to investigate the dynamic fractures of rocks under different static stress conditions.The effects of the loading rate and peak amplitude of the blasting wave under different confining pressures and the vertical compressive coefficient(K_(0))were considered.The numerical simulated results reproduced the initiation and further propagation of primary radial crack fractures,which were in agreement with the experimental results.The dynamic loading rate,peak amplitude,static vertical compressive coefficient(K_(0))and confining pressure affected the evolution of fractures around the borehole.The heterogeneity parameter(m)plays an important role in the evolution of fractures around the borehole.The crack propagation path became more discontinuous and rougher in a smallerheterogeneity parameter case.展开更多
This paper presents a brief summary of our recent work based on global MHD simulations of the Solar wind-Magnetosphere-Ionosphere (SMI) system with emphasis on the electrodynamic coupling in the system.The main conclu...This paper presents a brief summary of our recent work based on global MHD simulations of the Solar wind-Magnetosphere-Ionosphere (SMI) system with emphasis on the electrodynamic coupling in the system.The main conclusions obtained are summarized as follows.(1) As a main dynamo of the SMI system,the bow shock contributes to both region 1 Field-Aligned Current (FAC) and cross-tail current.Under strong interplanetary driving conditions and moderate Alfv'en Mach numbers,the bow shock's contribution may exceed more than fifty percent of the total of either region 1 or cross-tail currents.(2) In terms of more than 100 simulation runs with due southward Interplanetary Magnetic Field (IMF),we have found a combined parameter f=E sw P sw M A -1/2 (E sw,P sw,and M A are the solar wind electric field,ram pressure,and Alfv'en Mach number,respectively):both the ionospheric transpolar potential and the magnetopause reconnection voltage vary linearly with f for small f,but saturate for large f.(3) The reconnection voltage is approximately fitted by sin 3/2 (θ IMF /2),where θ IMF is the IMF clock angle.The ionospheric transpolar potential,the voltage along the polar cap boundary,and the electric fields along the merging line however defined they may be,respond differently to θ IMF,so it is not justified to take them as substitutes for the reconnection voltage.展开更多
Integrin activation,the transition from a low to a high affinity state,regulates the numerous cellular responses consequent to integrin engagement by extracellular matrix proteins.Kindlin proteins,play crucial roles i...Integrin activation,the transition from a low to a high affinity state,regulates the numerous cellular responses consequent to integrin engagement by extracellular matrix proteins.Kindlin proteins,play crucial roles in the integrin-signaling pathway by directly interacting with and activating integrins,which mediate the cell-extracellular matrix adhesion and signaling.As a widely distributed PTB domain protein and a major member of the kindlin family,kindlin2 interacts withβ3-tail,bridges talin-activated integrins to promote integrin aggregation,and enhances talin-induced integrin activation.Thus,kindlin2 is identified as a coactivator of integrins.Unlike talins,kindlin2 cannot directly alter the conformation of the integrin transmembrane helix and fail to activate integrin alone.Nevertheless,although it is widely accepted that kindlins and talins synergistically promote integrin activation,the underlying mechanism is unclear.Thus,the study of the force dissociation of the kindlin2/β3-tail complex and the conformation stabilization under different mechanical micro-environments should be of great significance for the further understanding of the structural basis of its synergistically activation of integrin.To reveal the molecular dynamics mechanism of interaction between kindlin2 andβ3-tail,we perform molecular dynamics(MD)simulations for this complex with different computing strategies interaction.In MD simulations,the available crystal structures of Kindlin-2/β3-tail complex(Protein Data Bank code 5XQ1)was downloaded from the PDB database.Two software packages,VMD for visualization and modeling and NAMD 2.13 for energy minimizations and MD simulations,were used here.The steadystate conformation of the complex was obtained from the equilibrium simulation.The dissociation event was observed by the constant velocity simulation,and the mechanical stability of the complex was observed by the constant force simulation.Our results showed that,during the equilibrium of the kindlin2-F3/β34ail complex,the residue MET612,LYS613 and TRP615 on the F3 domain of kindlin2 contributed to hydrogen-bonding with the corresponding residues onβ3 integrin.These bonds exhibit moderate or strong stability through steered molecular dynamics(SMD)simulation.During the constant velocity simulation,the complex exhibits a variety of unfolding pathways against tension applications,which are mainly distinguished by the disruption of hydrogen-bonds between the F3 domain a1/a2 helixes andβ1/β2 sheets.During the constant force simulation,the different phases of the composite force dissociation have different dissociation probabilities,which shows the biphasic force-dependent characteristics.And,the key residues in the pulling were recognized according not only to the number of interacting residue pairs,but also to their bond strength.Using molecular dynamics simulation,we showed the steady state of the kindlin2-F3/β3-tail complex under different tensile forces,and observe the dynamic process of molecular interaction.A possible underlying biophysical mechanism is that,the dissociation of Kindlin2-F3/β3-tail complex is biphasic force-dependent,and the conformations under different stretching states have different binding affinities.This study not only provides insights into the structural basis and mechanical regulation mechanisms of the kindlin/integrin interaction,in understanding in kindlin/integrin-related signaling in different cellular biological processes,but also provides new ideas for novel drug design and the treatment of related diseases.展开更多
The heated test pieces of diamond segments were treated by alternating magnetic field,the influences of magnetic treatment on microstructure densification of diamond segments were studied through metallurgical structu...The heated test pieces of diamond segments were treated by alternating magnetic field,the influences of magnetic treatment on microstructure densification of diamond segments were studied through metallurgical structure analysis.The experiment results indicated that,the densification of diamond segments was further improved after magnetized.The alternating magnetic force distributions in the diamond segments were calculated by numerical simulation according to the coupled field theory.In alternating magnetic field,a prodigious swirl current field appeared in the component.The magnetic vibrating due to alternating magnetic force was obvious,which was in favor of microcosmic structure compacter.The numerical analysis results provided direct evidences for that the alternating magnetic treatment can act as an effective technique to improve the microstructure densification of diamond segments.展开更多
In order to study the dynamic characteristics of the missile erection system,it can be considered as a rigid-flexible coupling multi-body system.Firstly,the actual system is abstracted as an equal and simplified one a...In order to study the dynamic characteristics of the missile erection system,it can be considered as a rigid-flexible coupling multi-body system.Firstly,the actual system is abstracted as an equal and simplified one and then the forces applied to it are analyzed.Secondly,the rigid-flexible coupling dynamic simulation for erection system is accomplished by use of the system simulation software,for example Pro/E,ADAMS,ANSYS,MATLAB/Simulink,etc.Finally,having the aid of simulation results,the kinetic and dynamic characteristics of the flexible bodies in erection system are analyzed.The simulation considering the erection system as a rigid-flexible coupling system can provide valuable results to the research of its kinetic,dynamic and vibrational characteristics.展开更多
文摘For space-borne gravitational wave detection missions based on the heterodyne interferometry principle,tilt-to-length(TTL)coupling noise is an important optical noise source,significantly influencing the accuracy of the measurement system.We present a method for analyzing TTL coupling noise under the joint influence of multiple factors.An equivalent simulated optical bench for the test mass interferometer was designed,and Gaussian beam tracing was adopted to simulate beam propagation.By simulating the interference signal,it can analyze the impact of various factors on the TTL coupling noise,including positional,beam parameters,detector parameters,and signal definition factors.On this basis,a random parameter space composed of multiple influential factors was constructed within a range satisfying the analysis requirement,and the corresponding simulation results from random sampling were evaluated via variance-based global sensitivity analysis.The calculated results of the main and total effect indexes show that the test mass rotation angle and the piston effect(lateral)significantly influence the TTL coupling noise in the test mass interferometer.The analysis provides a qualitative reference for designing and optimizing space-borne laser interferometry systems.
文摘During the propagation of high-power lasers within internal channels,the laser beam heats the propagation medium,causing the thermal blooming effect that degrades the beam quality at the output.The intricate configuration of the optical path within the internal channel necessitates complex and time-consuming efforts to assess the impact of thermal blooming effect on the optical path.To meet the engineering need for rapid evaluation of thermal blooming effect in optical paths,this study proposed a rapid simulation method for the thermal blooming effect in internal optical paths based on the finite element method.This method discretized the fluid region into infinitesimal elements and employed finite element method for flow field analysis.A simplified analytical model of the flow field region in complex internal channels was established,and regions with similar thermal blooming effect were divided within this model.Based on the calculated optical path differences within these regions,numerical simulations of phase distortion caused by thermal blooming were conducted.The calculated result were compared with those obtained using the existing methods.The findings reveal that for complex optical paths,the discrepancy between the two approaches is less than 3.6%,with similar phase distortion patterns observed.For L-type units,this method and the existing methods identify the same primary factors influencing aberrations and exhibit consistent trends in their variation.This method was used to analyze the impact of thermal blooming effect in a straight channel under different gravity directions.The results show that phase distortion varies with changes in the direction of gravity,and the magnitude of the phase difference is strongly correlated with the component of gravity perpendicular to the optical axis.Compared to the existing methods,this approach offers greater flexibility,obviates the need for complex custom analysis programming.The analytical results of this method enable a rapid assessment of the thermal blooming effect in optical paths within the internal channel.This is especially useful during the engineering design.These results also provide crucial references for developing strategies to suppress thermal blooming effect.
基金Project(20080431380) supported by China Postdoctoral Science Foundation
文摘Abstract： A joint solution model of variabk：-mass flow in two-phase region and fluid-solid coupling heat transfer, concerned about the charge process of variable-mass thermodynamic system, is built up and calculated by the finite element method （FEM）. The results are basically consistent with relative experimental data. The calculated average heat transfer coefficient reaches 1.7~105 W/（m2. K）. When the equal percentage valve is used, the system needs the minimum requirements of valve control, but brings the highest construction cost. With the： decrease of initial steam pressure, the heat transfer intensity also weakens but the steam flow increases. With the initial water filling coefficient increasing or the temperature of steam supply decreasing, the amount of accumulative steam flow increases with the growth of steam pressure. When the pressure of steam supply drops, the steam flow gradient increases during the maximum opening period of control valve, and causes the maximum steam flow to increase.
基金Project(2010CB732101)supported by the National Key Basic Research and Development Program of ChinaProjects(51079145,51379201)supported by the National Natural Science Foundation of China
文摘The pressure solution model of granular aggregates was introduced into a FEM code which was developed for the analysis of thermo-hydro-mechanical(T-H-M) coupling in porous medium. Aimed at creating a hypothetical model of nuclear waste disposal in unsaturated quartz aggregate rock mass with laboratory scale, two 4-year computation cases were designed: 1) The porosity and permeability of rock mass are functions of the pressure solution; 2) The porosity and the permeability are constants. Calculation results show that the magnitude and distribution of stresses in the rock mass of these two calculation cases are roughly the same. And, the porosity and the permeability decrease to 43%-54% and 4.4%-9.1% of their original values after case 1 being accomplished; but the negative pore water pressures in cases 1 and 2 are respectively 1.0-1.25 and 1.0-1.1 times of their initial values under the action of nuclear waste. Case 1 exhibits the obvious effect of pressure solution.
基金Project(2006AA03Z523) supported by the National High-Tech Research and Development Program of ChinaProject(08C26224302178) supported by the Innovation Foundation of Central South University,China
文摘To predict three-dimensional temperature distribution of molten aluminum and its influencing factors inside an industrial aluminum holding furnace,a fluid-solid coupled method was presented.The fluid-solid coupled mathematics models of aluminum holding furnace in the premixed combustion processing were established based on mass conservation,moment conservation,momentum conservation,energy conservation and chemistry species conservation.Computational results agree well with the test data of the typical condition.The maximum combustion temperature is 1 850 K.The average temperature of the molten aluminum is 1 158 K,and the maximum temperature difference is about 240 K.The average temperature increases 0.3 ℃ while the temperature of combustion air increases 1 ℃.The optimal excess air ratio is 1.25-1.30.
基金Projects(52074166,51774195,51704185)supported by the National Natural Science Foundation of ChinaProject(2019M652436)supported by the China Postdoctoral Science Foundation。
文摘In actual production,deep coal mine roadways are often under typical static-dynamic coupling stress(SDCS)conditions with high ground stress and strong dynamic disturbances.With the increasing number of disasters and accidents induced by SDCS conditions,the safe and efficient production of coal mines is seriously threatened.Therefore,it is of great practical significance to study the deformation and failure characteristics of the roadway surrounding rock under SDCS.In this paper,the effects of different in-situ stress fields and dynamic load conditions on the surrounding rock are studied by numerical simulations,and the deformation and failure characteristics are obtained.According to the simulation results,the horizontal stress,vertical stress and dynamic disturbance have a positive correlation with the plastic failure of the surrounding rock.Among these factors,the influence of the dynamic disturbance is the most substantial.Under the same stress conditions,the extents of deformation and plastic failure of the roof and ribs are always greater than those of the floor.The effect of horizontal stresses on the roadway deformation is more notable than that of vertical stresses.The results indicate that for the roadway under high-stress conditions,the in-situ stress test must be strengthened first.After determining the magnitude of the in-situ stress,the location of the roadway should be reasonably arranged in the design to optimize the mining sequence.For roadways that are strongly disturbed by dynamic loads,rock supports(rebar/cable bolts,steel set etc.)that are capable of maintaining their effectiveness without failure after certain dynamic loads are required.The results of this study contribute to understanding the characteristics of the roadway deformation and failure under SDCS,and can be used to provide a basis for the support design and optimization under similar geological and geotechnical circumstances.
基金Project(10772113) supported by the National Natural Science Foundation of China
文摘A complete geometric nonlinear formulation for rigid-flexible coupling dynamics of a flexible beam undergoing large overall motion was proposed based on virtual work principle, in which all the high-order terms related to coupling deformation were included in dynamic equations. Simulation examples of the flexible beam with prescribed rotation and free rotation were investigated. Numerical results show that the use of the first-order approximation coupling (FOAC) model may lead to a significant error when the flexible beam experiences large deformation or large deformation velocity. However, the correct solutions can always be obtained by using the present complete model. The difference in essence between this model and the FOAC model is revealed. These coupling high-order terms, which are ignored in FOAC model, have a remarkable effect on the dynamic behavior of the flexible body. Therefore, these terms should be included for the rigid-flexible dynamic modeling and analysis of flexible body undergoing motions with high speed.
基金Projects(51878190,51779031,51678170)supported by the National Natural Science Foundation of China。
文摘When underground cavities are subjected to explosive stress waves,a uniquely damaged zone may appear due to the combined effect of dynamic loading and static pre-load stress.In this study,a rate-dependent two-dimensional rock dynamic constitutive model was established to investigate the dynamic fractures of rocks under different static stress conditions.The effects of the loading rate and peak amplitude of the blasting wave under different confining pressures and the vertical compressive coefficient(K_(0))were considered.The numerical simulated results reproduced the initiation and further propagation of primary radial crack fractures,which were in agreement with the experimental results.The dynamic loading rate,peak amplitude,static vertical compressive coefficient(K_(0))and confining pressure affected the evolution of fractures around the borehole.The heterogeneity parameter(m)plays an important role in the evolution of fractures around the borehole.The crack propagation path became more discontinuous and rougher in a smallerheterogeneity parameter case.
基金Supported by grants NNSFC (40831060,40621003)NKBRSF G2006CB806304 in China
文摘This paper presents a brief summary of our recent work based on global MHD simulations of the Solar wind-Magnetosphere-Ionosphere (SMI) system with emphasis on the electrodynamic coupling in the system.The main conclusions obtained are summarized as follows.(1) As a main dynamo of the SMI system,the bow shock contributes to both region 1 Field-Aligned Current (FAC) and cross-tail current.Under strong interplanetary driving conditions and moderate Alfv'en Mach numbers,the bow shock's contribution may exceed more than fifty percent of the total of either region 1 or cross-tail currents.(2) In terms of more than 100 simulation runs with due southward Interplanetary Magnetic Field (IMF),we have found a combined parameter f=E sw P sw M A -1/2 (E sw,P sw,and M A are the solar wind electric field,ram pressure,and Alfv'en Mach number,respectively):both the ionospheric transpolar potential and the magnetopause reconnection voltage vary linearly with f for small f,but saturate for large f.(3) The reconnection voltage is approximately fitted by sin 3/2 (θ IMF /2),where θ IMF is the IMF clock angle.The ionospheric transpolar potential,the voltage along the polar cap boundary,and the electric fields along the merging line however defined they may be,respond differently to θ IMF,so it is not justified to take them as substitutes for the reconnection voltage.
基金supported by the National Natural Science Foundation of China ( 116272109, 11432006)
文摘Integrin activation,the transition from a low to a high affinity state,regulates the numerous cellular responses consequent to integrin engagement by extracellular matrix proteins.Kindlin proteins,play crucial roles in the integrin-signaling pathway by directly interacting with and activating integrins,which mediate the cell-extracellular matrix adhesion and signaling.As a widely distributed PTB domain protein and a major member of the kindlin family,kindlin2 interacts withβ3-tail,bridges talin-activated integrins to promote integrin aggregation,and enhances talin-induced integrin activation.Thus,kindlin2 is identified as a coactivator of integrins.Unlike talins,kindlin2 cannot directly alter the conformation of the integrin transmembrane helix and fail to activate integrin alone.Nevertheless,although it is widely accepted that kindlins and talins synergistically promote integrin activation,the underlying mechanism is unclear.Thus,the study of the force dissociation of the kindlin2/β3-tail complex and the conformation stabilization under different mechanical micro-environments should be of great significance for the further understanding of the structural basis of its synergistically activation of integrin.To reveal the molecular dynamics mechanism of interaction between kindlin2 andβ3-tail,we perform molecular dynamics(MD)simulations for this complex with different computing strategies interaction.In MD simulations,the available crystal structures of Kindlin-2/β3-tail complex(Protein Data Bank code 5XQ1)was downloaded from the PDB database.Two software packages,VMD for visualization and modeling and NAMD 2.13 for energy minimizations and MD simulations,were used here.The steadystate conformation of the complex was obtained from the equilibrium simulation.The dissociation event was observed by the constant velocity simulation,and the mechanical stability of the complex was observed by the constant force simulation.Our results showed that,during the equilibrium of the kindlin2-F3/β34ail complex,the residue MET612,LYS613 and TRP615 on the F3 domain of kindlin2 contributed to hydrogen-bonding with the corresponding residues onβ3 integrin.These bonds exhibit moderate or strong stability through steered molecular dynamics(SMD)simulation.During the constant velocity simulation,the complex exhibits a variety of unfolding pathways against tension applications,which are mainly distinguished by the disruption of hydrogen-bonds between the F3 domain a1/a2 helixes andβ1/β2 sheets.During the constant force simulation,the different phases of the composite force dissociation have different dissociation probabilities,which shows the biphasic force-dependent characteristics.And,the key residues in the pulling were recognized according not only to the number of interacting residue pairs,but also to their bond strength.Using molecular dynamics simulation,we showed the steady state of the kindlin2-F3/β3-tail complex under different tensile forces,and observe the dynamic process of molecular interaction.A possible underlying biophysical mechanism is that,the dissociation of Kindlin2-F3/β3-tail complex is biphasic force-dependent,and the conformations under different stretching states have different binding affinities.This study not only provides insights into the structural basis and mechanical regulation mechanisms of the kindlin/integrin interaction,in understanding in kindlin/integrin-related signaling in different cellular biological processes,but also provides new ideas for novel drug design and the treatment of related diseases.
基金supported by National Natural Science Foundation of China(50675190)
文摘The heated test pieces of diamond segments were treated by alternating magnetic field,the influences of magnetic treatment on microstructure densification of diamond segments were studied through metallurgical structure analysis.The experiment results indicated that,the densification of diamond segments was further improved after magnetized.The alternating magnetic force distributions in the diamond segments were calculated by numerical simulation according to the coupled field theory.In alternating magnetic field,a prodigious swirl current field appeared in the component.The magnetic vibrating due to alternating magnetic force was obvious,which was in favor of microcosmic structure compacter.The numerical analysis results provided direct evidences for that the alternating magnetic treatment can act as an effective technique to improve the microstructure densification of diamond segments.
文摘In order to study the dynamic characteristics of the missile erection system,it can be considered as a rigid-flexible coupling multi-body system.Firstly,the actual system is abstracted as an equal and simplified one and then the forces applied to it are analyzed.Secondly,the rigid-flexible coupling dynamic simulation for erection system is accomplished by use of the system simulation software,for example Pro/E,ADAMS,ANSYS,MATLAB/Simulink,etc.Finally,having the aid of simulation results,the kinetic and dynamic characteristics of the flexible bodies in erection system are analyzed.The simulation considering the erection system as a rigid-flexible coupling system can provide valuable results to the research of its kinetic,dynamic and vibrational characteristics.
