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Innovative pillar recovery method integrating gob-side entry driving and directional roof-cutting for thick-hard roof coal seams
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作者 WU Yi-yi YE Qiu-cheng +2 位作者 GAO Yu-bing ZHANG Xing-xing HE Man-chao 《Journal of Central South University》 2025年第9期3493-3513,共21页
To enhance the recuperation rate of the mine and comply with the stipulations of green mining technology, it is vital to expeditiously recuperate the coal pillar resources in the final stage, thus preventing the consi... To enhance the recuperation rate of the mine and comply with the stipulations of green mining technology, it is vital to expeditiously recuperate the coal pillar resources in the final stage, thus preventing the considerable squandering of resources. The coal pillar resource of the main roadway and its branch roadway constitutes a significant recovery subject. Its coal pillar shape is regular and possesses a considerable strike distance, facilitating the arrangement of the coal pillar recovery working face (CPRWF) for mining operations. However, for the remaining coal pillars with a thick and hard roof (THF) and multiple tectonic zones, CPRWF encounters challenges in selecting an appropriate layout, managing excessive roof pressure, and predicting mining stress. Aiming at the roadway coal pillar group with THF and multi-structural areas in specific projects, a method of constructing multi-stage CPRWF by one side gob-side entry driving (GSED) and one side roadway reusing is proposed. Through theoretical calculation of roof fracture and numerical simulation verification, combined with field engineering experience and economic analysis, the width of the narrow coal pillar (NCP) in the GSED is determined to be 10 m and the length of the CPRWF is 65 m. Concurrently, the potential safety hazard that the roof will fall asymmetrically and THF is difficult to break during CPRWF mining after GSED is analyzed and verified. Then, a control method involving the pre-cutting of the roof in the reused roadway before mining is proposed. This method has been shown to facilitate the complete collapse of THF, reduce the degree of mine pressure, and facilitate the symmetrical breaking of the roof. Accordingly, a roof-cutting scheme based on a directional drilling rig, bidirectional shaped polyvinyl chloride (PVC) pipe, and emulsion explosive was devised, and the pre-splitting of 8.2 m THF was accomplished. Field observations indicate that directional cracks are evident in the roof, the coal wall is flat during CPRWF mining, and the overall level of mining pressure is within the control range. Therefore, the combined application of GSED and roof-cutting technology for coal pillar recovery has been successfully implemented, thereby providing new insights and engineering references for the construction and pressure relief mining of CPRWF. 展开更多
关键词 coal pillar recovery thick and hard roof gob-side entry driving directional roof-cutting numerical analysis energy-gathering blasting
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Deformation mechanism and roof pre-splitting control technology of gob-side entry in thick hard main roof full-mechanized longwall caving panel 被引量:1
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作者 WANG Hao-sen HE Man-chao +6 位作者 WANG Jiong YANG Gang MAZi-min MING Can WANG Rui FENG Zeng-chao ZHANG Wen-jie 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第9期3206-3224,共19页
This paper explores the deformation mechanism and control technology of roof pre-splitting for gob-side entries in hard roof full-mechanized longwall caving panel(LTCC).The investigation utilizes a comprehensive appro... This paper explores the deformation mechanism and control technology of roof pre-splitting for gob-side entries in hard roof full-mechanized longwall caving panel(LTCC).The investigation utilizes a comprehensive approach that integrates field monitoring,theoretical analysis,and numerical simulation.Theoretical analysis has illuminated the influence of the length of the lateral cantilever beam of the main roof(LCBM)above the roadway on the stability of the gob-side entry behind the panel.Numerical simulations have further revealed that the longer LCBM results in heightened vertical stress within the coal pillar,developed cracks around the roadway,and more pronounced damage to the roadway.Moreover,numerical simulations also demonstrate the potential of roof pre-splitting technology in optimizing the fracture position of the hard roof.This technology significantly reduces the length of the LCBM,thereby alleviating stress concentration in the coal pillars and integrated coal rib while minimizing the destruction of the gob-side entry.Therefore,this manuscript first proposes the use of roof pre-splitting technology to control roadway deformation,and automatically retain the entry within a hard roof LTCC panel.Field implementation has demonstrated that the proposed automatically retained entry by roof pre-splitting technology effectively reduces gob-side entry deformation and achieves automatically retained entry. 展开更多
关键词 deformation mechanism hard roof gob-side entry cantilever beam roof pre-spliting
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Ground response and failure mechanism of gob-side entry by roof cutting with hard main roof 被引量:1
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作者 ZHU Heng-zhong XU Lei WEN Zhi-jie 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第7期2488-2512,共25页
This study is the result of long-term efforts of the authors’team to assess ground response of gob-side entry by roof cutting(GSERC)with hard main roof,aiming at scientific control for GSERC deformation.A comprehensi... This study is the result of long-term efforts of the authors’team to assess ground response of gob-side entry by roof cutting(GSERC)with hard main roof,aiming at scientific control for GSERC deformation.A comprehensive field measurement program was conducted to determine entry deformation,roof fracture zone,and anchor bolt(cable)loading.The results indicate that GSERC deformation presents asymmetric characteristics.The maximum convergence near roof cutting side is 458 mm during the primary use process and 1120 mm during the secondary reuse process.The entry deformation is closely associated with the primary development stage,primary use stage,and secondary reuse stage.The key block movement of roof cutting structure,a complex stress environment,and a mismatch in the supporting design scheme are the failure mechanism of GSERC.A controlling ideology for mining states,including regional and stage divisions,was proposed.Both dynamic and permanent support schemes have been implemented in the field.Engineering practice results indicate that the new support scheme can efficiently ensure long-term entry safety and could be a reliable approach for other engineering practices. 展开更多
关键词 gob-side entry by roof cutting ground response failure mechanism following mining states control hard main roof
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Superposed disturbance mechanism of sequential overlying strata collapse for gob-side entry retaining and corresponding control strategies 被引量:13
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作者 HAN Chang-liang ZHANG Nong +2 位作者 RAN Zhi GAO Rui YANG Hou-qiang 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第9期2258-2271,共14页
Gob-area roof rupture movement is a key disturbance factor for gob-side entry retaining.The characteristics of gob-area sequential roof collapse of overlying strata and superposed disturbance mechanism for gob-side en... Gob-area roof rupture movement is a key disturbance factor for gob-side entry retaining.The characteristics of gob-area sequential roof collapse of overlying strata and superposed disturbance mechanism for gob-side entry retaining are obtained via physical simulation and theoretical analysis,in which the scope of disturbed strata is enlarged from main roof to fracture zone.The experiment reveals that as a working face advances,roof strata sequentially collapse from bottom to top and produce multiple disturbances to gob-side entry retaining.Key strata among the overlying strata control each collapse.Main roof subsidence is divided into three stages:flexure subsidence prior to rupture,rotational subsidence during rupture and compressive subsidence after rupture.The amounts of deformation evident in each of the three stages are 15%,55%and 30%,respectively.After the master stratum collapses,main roof subsidence approaches its maximum value.The final span of the key stratum determines the moment and cycling of gob-side entry retaining disturbances.Main roof subsidence influences the load on the filling wall.The sequential roof collapse of overlying strata results in fluctuations in the gob-side entry retaining deformation.Calculation formulae for the final span of the key stratum and the filling wall load are obtained via theoretical analysis.A control method for the stability of the gob-side entry retaining’s surrounding rock is proposed,which includes 3 measures:a“dual-layer”proactive anchorage support,roadside filling with dynamic strength matching and auxiliary support during disturbance.Finally,the gob-side entry retaining of the Xiaoqing mine E1403 working face is presented as an engineering case capable of verifying the validity of the research conclusions. 展开更多
关键词 sequential roof collapse gob-side entry retaining superposed disturbance key stratum stability control
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Numerical simulation of calm water entry of flatted-bottom seafloor mining tool 被引量:4
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作者 胡小舟 刘少军 《Journal of Central South University》 SCIE EI CAS 2011年第3期658-665,共8页
The hydrodynamic problem of a two-dimensional model of seafloor mining tool entering still water vertically at constant speed was analyzed based on the velocity potential theory. For the assumption that the water entr... The hydrodynamic problem of a two-dimensional model of seafloor mining tool entering still water vertically at constant speed was analyzed based on the velocity potential theory. For the assumption that the water entry occurs with very short time interval, the viscosity and gravity of fluid were neglected. Considering the characteristic shape of it, the seafloor mining tool was simplified as a flat-bottom body. The governing equations were the Reynolds time-averaged equations and the k-e model. Finite element analysis was undertaken using the CFD software, Fluent. The impact pressures on the bottom of the mining tool were computed based on the improved volume of fuid method (VOF). The pressure distribution, the maximum impact pressure, and the impact duration time during the water entry of mining tool are presented at various deploying velocities, the two peak pressures in the impact process are observed, and the relationship between the maximum impact pressure and the deploying velocity is obtained. The results are compared with those based on other prediction theories and methods. 展开更多
关键词 water entry flatted-bottom body constant speed DEPLOYMENT
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Control effect of fluid entry pressure on hydrocarbon accumulation 被引量:3
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作者 聂海宽 张金川 《Journal of Central South University》 SCIE EI CAS 2010年第6期1395-1402,共8页
The micromigration of oil-drive-water and gas-drive-water in oil and gas fields was studied,by using core slices and micro-experimental technology,and the migration processes and characteristics of oil and gas in pore... The micromigration of oil-drive-water and gas-drive-water in oil and gas fields was studied,by using core slices and micro-experimental technology,and the migration processes and characteristics of oil and gas in pores and throats were observed,as well as entry pressures of oil/gas migration.Research shows that entry pressures of both oil-drive-water and gas-drive-water increase obviously as porosity decreases,and the statistical regularity observes the power function variation.However,there is a complex changing relationship between porosity and different entry pressure values of the two replacement processes,forming three curve sections,each representing a different accumulation significance.When the porosity is over 10%-12%,the difference between oil-drive-water and gas-drive-water entry pressures is small.Both oil and gas can migrate and accumulate in this kind of reservoir.The probabilities of oil and gas reservoir formation are nearly equal,forming conventional oil/gas pools.When porosity is between 5% and 10%-12%,the difference between the two is obvious,which indicates that this kind of reservoir can seal oil,but can also be a reservoir for gas,easily forming unconventional hydrocarbon pools(deep-basin gas pools).When porosity is less than 5%,the difference is indistinct and the entry capillary pressures show the same sealing function for oil and gas.In this condition,both oil and gas pools are difficult to form.Experimental results give a rational explanation for the difference of accumulation probability between deep-basin gas and deep-basin oil,and also for the mechanism of tight sand acting as cap rock. 展开更多
关键词 oil/gas-drive-water entry pressure micro-experiment secondary migration hydrocarbon accumulation
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Numerical investigation of water-entry of flatted-bottom seafloor mining tool in ocean waves 被引量:1
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作者 胡小舟 刘少军 《Journal of Central South University》 SCIE EI CAS 2014年第8期3071-3078,共8页
A numerical wave load model based on two-phase(water-air) Reynolds-Averaged Navier Stokes(RANS) type equations is used to evaluate hydrodynamic forces exerted on flatted-bottom seafloor mining tool during its entering... A numerical wave load model based on two-phase(water-air) Reynolds-Averaged Navier Stokes(RANS) type equations is used to evaluate hydrodynamic forces exerted on flatted-bottom seafloor mining tool during its entering ocean waves of deploying process.The discretization of the RANS equations is achieved by a finite volume approach(FV).The volume of fluid method(VOF) is employed to track the complicated free surface.A numerical wave tank is built to generate the ocean waves which are suitable for deploying seafloor mining tool.A typical deploying condition is employed to reflect the process of flatted-bottom body impacting with waves,and the pressure distribution of bottom is also presented.Four different lowering velocities are applied to obtain the time histories of maximum pressure of bottom,and it can be concluded that the pressure coefficient decreases with water velocity increasing,which is similar with ordinary water entry case.The numerical results clearly demonstrate the characteristics of flatted-bottom body entering ocean waves. 展开更多
关键词 flatted-bottom body water entry numerical wave tank DEPLOYMENT slamming load
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Optimal feedback based control for Mars entry trajectory tracking 被引量:1
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作者 HUANG Yuechen LI Haiyang SHEN Hongxin 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2018年第1期134-141,共8页
The various uncertainties of Mars environment have great impact on the process of vehicles entering the atmosphere.To improve the robustness of control system against the model errors and to reduce the computational b... The various uncertainties of Mars environment have great impact on the process of vehicles entering the atmosphere.To improve the robustness of control system against the model errors and to reduce the computational burden, an optimal feedback based tracking control law is developed. The control scheme presented in this paper determines the amplitude and the reversals of bank angle respectively in the longitudinal and lateral flight plane. At each control cycle, the amplitude of the bank angle is obtained by an optimal feedback controller to minimize tracking errors. The control gains are tuned according to the closed-loop error dynamics by using optimization methods. The bank reversals are executed if the crossrange exceeds a predetermined corridor which is designed by setting a boundary function. The accuracy and robustness of the proposed closed-loop optimal feedback based control law in tracking the reference trajectory is verified via500 deviation simulations, in which modeling errors and external disturbances are considered. 展开更多
关键词 optimal control feedback gains Mars entry trajectory tracking Pontryagin's minimum principle
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A New Entry to γ-Butenolides and trans-α,β-unsaturated Thioesters
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《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 1999年第S1期542-542,共1页
关键词 UNSATURATED A New entry to
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Let Accounting Function in Market Economy——Commemorating 500th Anniversary Of The Publication Of Pacioli's On Double-Entry Bookkeeping
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作者 Zhongnan University of Finance and Economics Yang Shizhan 《财会通讯(上)》 1994年第S1期23-25,共3页
In 1494, ’Summa de Arithmetica, Geometria, Proportion et Proportionalita’ was published by Luca Pacioli (1445?——1514?). This book has five parts. Accountants pay a great deal of attention to the third part entitl... In 1494, ’Summa de Arithmetica, Geometria, Proportion et Proportionalita’ was published by Luca Pacioli (1445?——1514?). This book has five parts. Accountants pay a great deal of attention to the third part entitled ’Particulars de Computis et Scripturis’ or 展开更多
关键词 PUBLICATION entitled today evaluating OWNER 饭心 entry Columbus HUMANITY himself
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L. Pacioli's Historic Contributions to Double Entry Bookkeeping Laid A Solid Foundation for the Continuous Development of Accounting Science in the World Ever Since(Abstract in Detail)
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作者 Xiamen University Yu Xuying 《财会通讯(上)》 1994年第S1期41-43,共3页
Ⅰ 1994 is a commemorable year for accounting aca demies and practitioners all over the world. They hold in high esteem to celebrate the 500th anniversary of the publication of L. Pacioli’s epoch marking work Particu... Ⅰ 1994 is a commemorable year for accounting aca demies and practitioners all over the world. They hold in high esteem to celebrate the 500th anniversary of the publication of L. Pacioli’s epoch marking work Particularization of Computation and Recordation (Bookkeeping Theory). L. Pacioli has gone down in history as the p(?)oneer in the development of the modern accounting. 展开更多
关键词 ACCOUNTING celebrate marking EPOCH anniversary creative keeping entry originally preparing
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Double-entry Bookkeeping and Paciolo's Bookkeeping Theory: Dedicated to the 500th Anniversary of the Publication of Paciolo's Treatise on Bookkeeping
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作者 Xiamen University Ge Jiashu 《财会通讯(上)》 1994年第S1期10-13,共4页
In 1494, Luca Paciolo,the Father of Modern Accounting, published his famous work, SUMMA DE ARITHMETICA, GEOMETRIA, PROPORTIONI ET PROPORTIONALITA (hereinafter referred to as the SUMMA). In Chapter 3 of the SUMMA,"... In 1494, Luca Paciolo,the Father of Modern Accounting, published his famous work, SUMMA DE ARITHMETICA, GEOMETRIA, PROPORTIONI ET PROPORTIONALITA (hereinafter referred to as the SUMMA). In Chapter 3 of the SUMMA,"Particulatis De Computis et Scripturis" (Details of Accounting and Recording), the author systematically introduced 展开更多
关键词 PUBLICATION entry systematically hereinafter GOLDEN AHEAD today keeping TRADING agreed
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Experimental study on the anti-penetration characteristics of liquidfilled structure with air layer
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作者 Mengmeng Wu Jian Jin Hailiang Hou 《Defence Technology(防务技术)》 2025年第8期185-202,共18页
The study aims to explore the damage characteristics and protection technologies of liquid-filled structures under high-speed projectile impact.A series of penetration impact experiments were conducted by focusing on ... The study aims to explore the damage characteristics and protection technologies of liquid-filled structures under high-speed projectile impact.A series of penetration impact experiments were conducted by focusing on different air layer configurations.By using high-speed camera and dynamic measurement systems,the effects of air layers on the projectile penetration,pressure wave propagation,cavitation evolution,and structural dynamic responses were analyzed.The results showed that the rarefaction wave reflected from the air-liquid interface significantly reduced the peak and specific impulse of the initial pressure wave,thereby diminishing the impact load on the structure.Additionally,the compressibility of air layers also attenuated the cavitation extrusion load.Both front and rear plates exhibited superimposed deformation modes,i.e.,local deformation or petal fracture with global deformation.Air layers effectively mitigated global deformation.However,when the air layer was positioned on the projectile's trajectory,it split the water-entry process and velocity attenuation of the projectile into two relatively independent phases.And the secondary water entry pressure wave caused more severe local deformation and petal fractures on the rear plate. 展开更多
关键词 Liquid-filled structure Air layer Water entry PROJECTILE PENETRATION Pressure wave
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Entire aerial-aquatic trajectory modeling and optimization for trans- medium vehicles
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作者 Teng Long Nianhui Ye +2 位作者 Baoshou Zhang Jingliang Sun Renhe Shi 《Defence Technology(防务技术)》 2025年第7期223-241,共19页
Trans-medium flight vehicles can combine high aerial maneuverability and underwater concealment ability,which have attracted much attention recently.As the most crucial procedure,the trajectory design generally determ... Trans-medium flight vehicles can combine high aerial maneuverability and underwater concealment ability,which have attracted much attention recently.As the most crucial procedure,the trajectory design generally determines the trans-medium flight vehicle performance.To quantitatively analyze the flight vehicle performance,an entire aerial-aquatic trajectory model is developed in this paper.Different from modeling a trajectory purely for the water entry process,the constructed entire trajectory model has integrated aerial,water entry,and underwater trajectories together,which can consider the influence of the connected trajectories.As for the aerial and underwater trajectories,explicit dynamic models are established to obtain the trajectory parameters.Due to the complicated fluid force during high-velocity water entry,a computational fluid dynamics model is investigated to analyze this phase.The compu-tational domain size is adaptively refined according to the final aerial trajectory state,where the redundant computational domain is removed.An entire trajectory optimization problem is then formulated to maximize the total flight range via tuning the joint states of different trajectories.Simultaneously,several constraints,i.e.,the max impact load,trajectory height,etc.,are involved in the optimization problem.Rather than directly optimizing by a heuristic algorithm,a multi-surrogate cooperative sampling-based optimization method is proposed to alleviate the computational complexity of the entire trajectory optimization problem.In this method,various surrogates coopera-tively generate infill sample points,thereby preventing the poor approximation.After optimization,the total flight range can be improved by 20%,while all the constraints are satisfied.The result demonstrates the effectiveness and practicability of the developed model and optimization framework. 展开更多
关键词 Water entry Trans-medium vehicle Computational fluid dynamics Trajectory optimization Pseudospectral method Surrogate
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An innovative N00 mining approach for protecting entries and mining panels
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作者 ZHANG Jun HE Man-chao +7 位作者 WANG Ya-jun YANG Gang HOU Shi-lin CHEN Yu-wen KANG Xu-hui SHI Zhen FU Qiang DU Fu-kang 《Journal of Central South University》 2025年第9期3514-3538,共25页
Addressing the issues of significant entry settlement and severe mining pressure manifestations in the conventional 121 approach,an innovative N00 approach is proposed.By comparing the mining process and entry formati... Addressing the issues of significant entry settlement and severe mining pressure manifestations in the conventional 121 approach,an innovative N00 approach is proposed.By comparing the mining process and entry formation process of different approaches,the characteristics of entry roof settlement evolution under different approaches are obtained.The N00 approach,which incorporates roof cutting and NPR cable support,optimizes the mining and entry formation process to reduce the settlement phase of entry roof,decreases the settlement of entry roof,and enhances the steadiness of entry roof.The N00 approach modifies the entry roof structure through roof cutting and establishes a hydraulic support load mechanics model for the mining panel to derive the theoretical load pressure formula for the N00 approach’s hydraulic support.Compared with the conventional 121 approach,the pressure on the N00 approach’s hydraulic support is reduced.Empirical data obtained through field monitoring demonstrate that the N00 approach has reduced the roof settlement of the entry and weakened the mining pressure manifestation at the mining panel,achieving the goal of protecting the entry and mining panel. 展开更多
关键词 N00 approach entry formation process roof cutting roof settlement mining pressure manifestation
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Numerical study on the cavity dynamics for vertical water entries of twin spheres 被引量:2
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作者 Xu Wang Xujian Lyu +1 位作者 Ruisheng Sun Dongdong Tang 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第2期459-472,共14页
In this study, a three dimensional(3D) numerical model of six-degrees-of-freedom(6DOF) is applied to simulate the water entries of twin spheres side-by-side at different lateral distances and time intervals.The turbul... In this study, a three dimensional(3D) numerical model of six-degrees-of-freedom(6DOF) is applied to simulate the water entries of twin spheres side-by-side at different lateral distances and time intervals.The turbulence structure is described using the shear-stress transport k-ω(SST k-ω) model, and the volume of fluid(VOF) method is used to track the complex air-liquid interface. The motion of spheres during water entry is simulated using an independent overset grid. The numerical model is verified by comparing the cavity evolution results from simulations and experiments. Numerical results reveal that the time interval between the twin water entries evidently affects cavity expansion and contraction behaviors in the radial direction. However, this influence is significantly weakened by increasing the lateral distance between the two spheres. In synchronous water entries, pressure is reduced on the midline of two cavities during surface closure, which is directly related to the cavity volume. The evolution of vortexes inside the two cavities is analyzed using a velocity vector field, which is affected by the lateral distance and time interval of water entries. 展开更多
关键词 Twin water entries Side-by-side CAVITY Numerical simulation
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Research and application of mechanical models for the whole process of 110 mining method roof structural movement 被引量:12
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作者 BIAN Wen-hui YANG Jun +2 位作者 HE Man-chao ZHU Chun XU Dong-ming 《Journal of Central South University》 SCIE EI CAS CSCD 2022年第9期3106-3124,共19页
For the 110 mining method,it is challenging to accurately calculate the support resistance of the roadway due to the lack of understanding of the dynamic movement of the overlying strata in this method.The consequenti... For the 110 mining method,it is challenging to accurately calculate the support resistance of the roadway due to the lack of understanding of the dynamic movement of the overlying strata in this method.The consequential excessive support results in a significant increase in the cost of roadway support.The authors explored the overlying strata movement and roadway deformation of the gob-entry retaining in the 110 mining method to solve this problem.First,the typical stages of the roof-cutting gob-side entry were defined.Second,the mechanical model and calculation formula of the support resistance on the roof were explored.Then,using numerical simulation software,the starting ranges of the specific supports at different stages were verified and the feasibility of the support scheme was examined.Finally,combined with the field measurement data,the stress and the deformation of the gob roadway at different stages under the influence of two mining processes in the 110 mining method were obtained.The numerical simulation results obtained are consistent with the field test results,providing a theoretical basis for precision support at different stages by the 110 mining method. 展开更多
关键词 110 mining method gob-side entry retaining roof structure movement mechanical model
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The construction of polycistronic retroviral vector
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作者 Guan-Hua Lai, Shi-Shu ChenResearch Center for Human Gene Therapy, ShanghaiSecond Medical University, Shanghai 200025 《中国实验血液学杂志》 CAS CSCD 1997年第3期332-332,共1页
Objective: In order to coordinately express amarker gene and therapeutic genes in one vector, apolycistronic retroviral vector was constructed withthe internal ribosome entry site from encephalo-myocarditis and polio ... Objective: In order to coordinately express amarker gene and therapeutic genes in one vector, apolycistronic retroviral vector was constructed withthe internal ribosome entry site from encephalo-myocarditis and polio virus, which was able to expressthree different genes simultaneously. Methods: Thevector used mouse moloney leukemia virus sequencesfor viral expression and packaging functions 展开更多
关键词 RIBOSOME encephalo packaging entry MYOCARDITIS HEPATOMA PRODUCER TITER PROMOTER capable
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Experimental investigation of clam water impact on flatted-bottom seafloor mining tool
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作者 胡小舟 刘少军 《Journal of Central South University》 SCIE EI CAS 2014年第2期533-539,共7页
A test rig for constant velocity water entry experiments was developed that drives a flatted-bottom section attached on six degree of freedom(6-DOF) platform to enter the water vertically at near constant velocity.The... A test rig for constant velocity water entry experiments was developed that drives a flatted-bottom section attached on six degree of freedom(6-DOF) platform to enter the water vertically at near constant velocity.The experiment system,which consists of drive and actuation system,water pool,model test sections,load cell,and control system,was presented.Water entry forces of different velocities were measured during impact process,and for each test case,three runs were performed with the same motion program to check the repeatability of the force readings.The experiment results are compared with two-dimensional(2D) CFD simulation methods for flatted-bottom rigid bodies with constant entry velocity.Experimental results indicate that the impact forces mainly depend on water entry velocities.It is concluded that the feasibility and accuracy of simulation methods has been validated. 展开更多
关键词 water entry water impact constant velocity DEPLOYMENT
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Effects of total flavonoids of Rhododendra simsii on ameliorating brain injury via G protein-coupled SOCE pathway mediated by STIM and Orai in subacute phase of ischemia/reperfusion
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作者 LU Jia-jun JIANG Chen-chen +5 位作者 HE Yu-xiang SHI Lei YIN Xiu-yun CHEN Zhuo CAO Di HAN Jun 《中国药理学与毒理学杂志》 CAS 北大核心 2021年第10期768-769,共2页
OBJECTIVE To explore the effect of total flavonoids of Rhododendra simsii(TFR)on improving cerebral ischemia/reperfusion injury(CIRI)and its relationship with STIM/Orai-regulated operational Ca^(2+)influx(SOCE)pathway... OBJECTIVE To explore the effect of total flavonoids of Rhododendra simsii(TFR)on improving cerebral ischemia/reperfusion injury(CIRI)and its relationship with STIM/Orai-regulated operational Ca^(2+)influx(SOCE)pathway.METHODS Oxygen-glucose deprivation/reoxygenation(OGD/R)PC12 cells were used to simulate CIRI in vitro,and the intracellular Ca^(2+)concentration and apoptosis rate of PC12 cells were detected by laser confocal microscope and flow cytometry,respectively.The regulation of STIM/Orai on SOCE was analyzed by STIM/Orai gene silencing and STIM/O rai gene overexpression.The CIRI model was established by MCAO in SD rats.The activities of inflammatory cytokines IL^(-1),IL-6 and TNF-αin serum were detected by ELISA.The pathological changes of ischemic brain tissue and the infarction of rat brain tissue were detected by HE staining and TTC staining.The protein and mRNA expression levels of STIM1,STIM2,Orai1,caspase-3 and PKB in brain tissue were detected by Western blotting and RT-qPCR,respectively.RESULTS The results of in vitro experiment showed that the fluorescence intensity of Ca^(2+)and apoptosis rate in PC12 cells treated with TFR were significantly lower than those in OGD/R group,and this trend was enhanced by SOCE antagonist 2-APB.STIM1/STIM2/Orai1 gene silencing significantly reduced apoptosis and Ca^(2+)overload in OGD/R model,while TFR combined with overexpression of STIM1/STIM2/Orai1 aggravated apoptosis and Ca2+overload.In the in vivo experiment,TFR significantly reduced the brain histopathological damage,infarction of brain tissue,the contents of IL^(-1),IL-6 and TNF-αin the serum in MCAO rats and down-regulated the expression of STIM1,STIM2,Orai1 and caspase-3 protein and mRNA in the brain tissue,and up-regulated the expression of PKB.The above effects were enhanced by the addition of 2-APB.CONCLUSION The above results indicate that TFR may reduce the contents of inflammatory factors and apoptosis,decrease Ca2+overload and ameliorate brain injury by inhibiting SOCE pathway mediated by STIM and Orai,suggesting that it has a protective effect against subacute CIRI. 展开更多
关键词 total flavonoids of Rhododendra simsii cerebral ischemia/reperfusion injury STIM/Orai store-operated calcium entry 2-APB
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