The fertilization and development process of embryo and endosperm of strawberry, Gelila snd Xiaoshi which were broadly cultivated in Harbin were investigated by optical microscopy、fluorescence microscopy and transmis...The fertilization and development process of embryo and endosperm of strawberry, Gelila snd Xiaoshi which were broadly cultivated in Harbin were investigated by optical microscopy、fluorescence microscopy and transmission electron microscopy. The papilla cells ranked in grid orid on its stigma and the flourishing transmitting tissue appeared in the center of its thin and long pistillary. There was only one crassinucellate hemianatropous ovule with Polygonum type embryo sac and single integument in each ovary. The germination of pollen and the pathway of pollen tube growth could be clearly observed with fluorescence microscopy: stigma→transmitting tissue in pistillary→intine of ovary placenta→the surface of ovule→micropyle. The development of embryo was consistent with most dicotyledons : zygote→2-cell proembryo→proembryo shaped like a ball→proembryo shaped like a heart→proembryo shaped like a torpedo→proembryo formed two cotyledons. The embryo was orthotropous and its development was asterad type. The endosperm development belonged to nuclear type as following: primary endosperm nucleus→free endosperm nucleus→endosperm cell→endosperm assimilated gradually by embryo. There was a special structure around it during the development of Gelila embryo.展开更多
文摘The fertilization and development process of embryo and endosperm of strawberry, Gelila snd Xiaoshi which were broadly cultivated in Harbin were investigated by optical microscopy、fluorescence microscopy and transmission electron microscopy. The papilla cells ranked in grid orid on its stigma and the flourishing transmitting tissue appeared in the center of its thin and long pistillary. There was only one crassinucellate hemianatropous ovule with Polygonum type embryo sac and single integument in each ovary. The germination of pollen and the pathway of pollen tube growth could be clearly observed with fluorescence microscopy: stigma→transmitting tissue in pistillary→intine of ovary placenta→the surface of ovule→micropyle. The development of embryo was consistent with most dicotyledons : zygote→2-cell proembryo→proembryo shaped like a ball→proembryo shaped like a heart→proembryo shaped like a torpedo→proembryo formed two cotyledons. The embryo was orthotropous and its development was asterad type. The endosperm development belonged to nuclear type as following: primary endosperm nucleus→free endosperm nucleus→endosperm cell→endosperm assimilated gradually by embryo. There was a special structure around it during the development of Gelila embryo.