The interaction between domoic acid(DA) and 18-mer double-stranded DNA(dsDNA) was investigated by using microchip-based non-gel sieving electrophoresis. For the 18-mer dsDNA with a sequence of 3′-GCATTGGTTGACGTTGCA-5...The interaction between domoic acid(DA) and 18-mer double-stranded DNA(dsDNA) was investigated by using microchip-based non-gel sieving electrophoresis. For the 18-mer dsDNA with a sequence of 3′-GCATTGGTTGACGTTGCA-5′, the amount of free dsDNA decreases with the increase of the added DA and also with the increase of reactive time. Meanwhile, the newly-produced two peaks were observed. The experimental results show that there is a strong interaction between DA and the 18-mer dsDNA. DA makes the degradation of the 18-mer dsDNA. The non-gel sieving electrophoresis on microchip is a rapid, convenient, highly sensitive method for studying the interaction between DNA and small molecules.展开更多
为了丰富我国海域拟菱形藻(Pseudo-nitzschia Peragallo)的物种多样性,并澄清其产毒特征,研究从广东大亚湾海域分离并建立了一株拟菱形藻的单克隆培养株系MC298,通过光学显微镜下的群体特征和透射电镜下的超微形态特征观察,结合基于核...为了丰富我国海域拟菱形藻(Pseudo-nitzschia Peragallo)的物种多样性,并澄清其产毒特征,研究从广东大亚湾海域分离并建立了一株拟菱形藻的单克隆培养株系MC298,通过光学显微镜下的群体特征和透射电镜下的超微形态特征观察,结合基于核糖体转录间隔区(Internal Transcribed Spacer,ITS)的分子系统学数据,以及基于ITS2转录RNA的二级结构分析,鉴定到我国拟菱形藻属的1个新记录种:并基拟菱形藻P.decipiens Lundholm&Moestrup。研究对其形态学特征进行了较为详细地描述,并与相似种进行了比较,还对其ITS2-RNA的特有标志结构进行了阐述。同时,利用高效液相色谱-质谱联用法(Liquid chromatography tandem mass spectrometry,LC-MS/MS)对该藻株的产毒特征进行了检测,结果未检测到DA的存在。研究不仅丰富了我国拟菱形藻属的物种多样性,也可为拟菱形藻的产毒特征研究提供基础数据。展开更多
文摘The interaction between domoic acid(DA) and 18-mer double-stranded DNA(dsDNA) was investigated by using microchip-based non-gel sieving electrophoresis. For the 18-mer dsDNA with a sequence of 3′-GCATTGGTTGACGTTGCA-5′, the amount of free dsDNA decreases with the increase of the added DA and also with the increase of reactive time. Meanwhile, the newly-produced two peaks were observed. The experimental results show that there is a strong interaction between DA and the 18-mer dsDNA. DA makes the degradation of the 18-mer dsDNA. The non-gel sieving electrophoresis on microchip is a rapid, convenient, highly sensitive method for studying the interaction between DNA and small molecules.
文摘为了丰富我国海域拟菱形藻(Pseudo-nitzschia Peragallo)的物种多样性,并澄清其产毒特征,研究从广东大亚湾海域分离并建立了一株拟菱形藻的单克隆培养株系MC298,通过光学显微镜下的群体特征和透射电镜下的超微形态特征观察,结合基于核糖体转录间隔区(Internal Transcribed Spacer,ITS)的分子系统学数据,以及基于ITS2转录RNA的二级结构分析,鉴定到我国拟菱形藻属的1个新记录种:并基拟菱形藻P.decipiens Lundholm&Moestrup。研究对其形态学特征进行了较为详细地描述,并与相似种进行了比较,还对其ITS2-RNA的特有标志结构进行了阐述。同时,利用高效液相色谱-质谱联用法(Liquid chromatography tandem mass spectrometry,LC-MS/MS)对该藻株的产毒特征进行了检测,结果未检测到DA的存在。研究不仅丰富了我国拟菱形藻属的物种多样性,也可为拟菱形藻的产毒特征研究提供基础数据。
