The Nei's improved genetic distance(DA)and gene flow(Nm)were measured using sixteen microsatellite markers.Dendograms based on DA genetic distance using the neighbor-joining(NJ)method and STRUCTURE program were co...The Nei's improved genetic distance(DA)and gene flow(Nm)were measured using sixteen microsatellite markers.Dendograms based on DA genetic distance using the neighbor-joining(NJ)method and STRUCTURE program were constructed to analyze the genetic structure and relationship among 10 Chinese indigenous chicken breeds.The results showed that dendograms of DA genetic distance using the NJ method divided the 10 chicken breeds into two main clusters;one consisted of breeds of low weight body(CHA,TTB,XIA,GUS and BAI),the other contained heavier breeds(LAN,DAG,YOU,XIS and LUY).In the lighter breeds,TIB and CHA clustered together,as did XIA and GUS.In the heavier breeds,XIS and LUY was clustered together in one branch,but LAN,DAG and YOU clustered in independent branches.The results were consistent with Nm estimates among the 10 indigenous chicken breeds.The STRUCTURE program properly inferred the presence of genetic structure despite not pre-defining the origin of individuals.The genetic cluster inferred by STRUCTURE was basically the same as that from the DA distance clustering method.An advantage of the STRUCTURE program was its ability to identify the migrants and admixed individuals in the 10 chicken populations;this could not be achieved by use of the DA distance clustering method.展开更多
Congestion pricing is an important component of urban intelligent transport system.The efficiency,equity and the environmental impacts associated with road pricing schemes are key issues that should be considered befo...Congestion pricing is an important component of urban intelligent transport system.The efficiency,equity and the environmental impacts associated with road pricing schemes are key issues that should be considered before such schemes are implemented.This paper focuses on the cordon-based pricing with distance tolls,where the tolls are determined by a nonlinear function of a vehicles' travel distance within a cordon,termed as toll charge function.The optimal tolls can give rise to:1) higher total social benefits,2) better levels of equity,and 3) reduced environmental impacts(e.g.,less emission).Firstly,a deterministic equilibrium(DUE) model with elastic demand is presented to evaluate any given toll charge function.The distance tolls are non-additive,thus a modified path-based gradient projection algorithm is developed to solve the DUE model.Then,to quantitatively measure the equity level of each toll charge function,the Gini coefficient is adopted to measure the equity level of the flows in the entire transport network based on equilibrium flows.The total emission level is used to reflect the impacts of distance tolls on the environment.With these two indexes/measurements for the efficiency,equity and environmental issues as well as the DUE model,a multi-objective bi-level programming model is then developed to determine optimal distance tolls.The multi-objective model is converted to a single level model using the goal programming.A genetic algorithm(GA) is adopted to determine solutions.Finally,a numerical example is presented to verify the methodology.展开更多
基金supported by the Program of National Technological Basis from Ministry of Science and Technology of China(No.2005DKA21101)the National Natural Science Foundation of China(No.30700572)
文摘The Nei's improved genetic distance(DA)and gene flow(Nm)were measured using sixteen microsatellite markers.Dendograms based on DA genetic distance using the neighbor-joining(NJ)method and STRUCTURE program were constructed to analyze the genetic structure and relationship among 10 Chinese indigenous chicken breeds.The results showed that dendograms of DA genetic distance using the NJ method divided the 10 chicken breeds into two main clusters;one consisted of breeds of low weight body(CHA,TTB,XIA,GUS and BAI),the other contained heavier breeds(LAN,DAG,YOU,XIS and LUY).In the lighter breeds,TIB and CHA clustered together,as did XIA and GUS.In the heavier breeds,XIS and LUY was clustered together in one branch,but LAN,DAG and YOU clustered in independent branches.The results were consistent with Nm estimates among the 10 indigenous chicken breeds.The STRUCTURE program properly inferred the presence of genetic structure despite not pre-defining the origin of individuals.The genetic cluster inferred by STRUCTURE was basically the same as that from the DA distance clustering method.An advantage of the STRUCTURE program was its ability to identify the migrants and admixed individuals in the 10 chicken populations;this could not be achieved by use of the DA distance clustering method.
基金Projects (61304198,61374195) supported by the National Natural Science Foundation of ChinaProjects (2013M530159,2014T70351) supported by the China Postdoctoral Science Foundation
文摘Congestion pricing is an important component of urban intelligent transport system.The efficiency,equity and the environmental impacts associated with road pricing schemes are key issues that should be considered before such schemes are implemented.This paper focuses on the cordon-based pricing with distance tolls,where the tolls are determined by a nonlinear function of a vehicles' travel distance within a cordon,termed as toll charge function.The optimal tolls can give rise to:1) higher total social benefits,2) better levels of equity,and 3) reduced environmental impacts(e.g.,less emission).Firstly,a deterministic equilibrium(DUE) model with elastic demand is presented to evaluate any given toll charge function.The distance tolls are non-additive,thus a modified path-based gradient projection algorithm is developed to solve the DUE model.Then,to quantitatively measure the equity level of each toll charge function,the Gini coefficient is adopted to measure the equity level of the flows in the entire transport network based on equilibrium flows.The total emission level is used to reflect the impacts of distance tolls on the environment.With these two indexes/measurements for the efficiency,equity and environmental issues as well as the DUE model,a multi-objective bi-level programming model is then developed to determine optimal distance tolls.The multi-objective model is converted to a single level model using the goal programming.A genetic algorithm(GA) is adopted to determine solutions.Finally,a numerical example is presented to verify the methodology.