OBJECTIVE To investigate the effects of Radix Cynanchum bungei extract(RCBE) on cerebral ischemia-reperfusion(I/R) and acute cerebral ischemia induced impairment in mice.METHODS I/R model was induced by bilateral caro...OBJECTIVE To investigate the effects of Radix Cynanchum bungei extract(RCBE) on cerebral ischemia-reperfusion(I/R) and acute cerebral ischemia induced impairment in mice.METHODS I/R model was induced by bilateral carotid artery occlusion(BCAO)-reperfusion method and Y-maze learning and memory performance was assessed after reperfusion. Na^+-K^+-ATPase,Ca^(2+)-ATPase and SOD activity,as well as MDA content in mouse brain tissue were measured. Numbers of mouth-opening breaths of the isolated mouse head were observed in acute cerebral ischemia mice.RESULTS Learning and memory ability in mice with RCBE were improved significantly compared with model group. The activity of SOD,Na^+-K^+-ATPase and Ca^(2+)-ATPase were increased,while MDA contents decreased after RCBE(0.5,1.0 and 2.0 g·kg^(-1)) and piracetam(0.5 g·kg^(-1)) treatment compared with the model group. RCBE at all concentrations significantly prolonged the number of mouth-opening breaths of the isolated mouse head. CONCLUSION RCBE preconditioning exerts a marked neuroprotective effect on the ischemia brain,which is related to improve the learning and memory via regulating energy metabolism and anti-oxidation.展开更多
为明确影响鹅绒藤分布的主导环境因子及其生态阈值,量化不同时期其潜在适生区,借助MaxEnt模型与ArcGIS空间分析技术,融合鹅绒藤在我国的301个分布点位信息及生物气候数据,对其在2个过去时期[末次盛冰期(约22000 a前)和全新世中期(约600...为明确影响鹅绒藤分布的主导环境因子及其生态阈值,量化不同时期其潜在适生区,借助MaxEnt模型与ArcGIS空间分析技术,融合鹅绒藤在我国的301个分布点位信息及生物气候数据,对其在2个过去时期[末次盛冰期(约22000 a前)和全新世中期(约6000 a前)]、当前时期(1970—2000年)以及3个未来时期[2030s(2021—2040年)、2050s(2041—2060年)、2070s(2061—2080年)]的潜在适生区进行预测分析。结果表明,MaxEnt模型各时期受试者工作特征曲线下面积(Area under the curve,AUC)值均高于0.9,预测结果可靠。鹅绒藤的分布主要受年平均温度、温度季节性变异系数、降水量季节性变异系数和最湿月份降水量影响。当前时期下,鹅绒藤的总适生区面积达193.37675×10^(4)km^(2),其中高适生区面积为69.01563×10^(4)km^(2)。与当前时期相对比,鹅绒藤在末次盛冰期和全新世中期适生区扩张幅度较为显著。3个未来时期中,鹅绒藤在SSP370(区域竞争路径)气候变化情景下2070s适生区面积最大,在SSP126(可持续路径)气候变化情景下2030s适生区面积最小,但均比当前时期适生区面积有所扩张。综上,鹅绒藤在2个过去时期和3个未来时期不同气候情景下均呈现扩张趋势,反映了气候变化,尤其是碳循环所引起的温度变化对物种分布的阶段性影响。展开更多
基金The project supported by Medical Science and Technology Development Plan of Shandong Province(2016WS0611)Science and Technology Development Plan of Tai'an City(2016NS1070)
文摘OBJECTIVE To investigate the effects of Radix Cynanchum bungei extract(RCBE) on cerebral ischemia-reperfusion(I/R) and acute cerebral ischemia induced impairment in mice.METHODS I/R model was induced by bilateral carotid artery occlusion(BCAO)-reperfusion method and Y-maze learning and memory performance was assessed after reperfusion. Na^+-K^+-ATPase,Ca^(2+)-ATPase and SOD activity,as well as MDA content in mouse brain tissue were measured. Numbers of mouth-opening breaths of the isolated mouse head were observed in acute cerebral ischemia mice.RESULTS Learning and memory ability in mice with RCBE were improved significantly compared with model group. The activity of SOD,Na^+-K^+-ATPase and Ca^(2+)-ATPase were increased,while MDA contents decreased after RCBE(0.5,1.0 and 2.0 g·kg^(-1)) and piracetam(0.5 g·kg^(-1)) treatment compared with the model group. RCBE at all concentrations significantly prolonged the number of mouth-opening breaths of the isolated mouse head. CONCLUSION RCBE preconditioning exerts a marked neuroprotective effect on the ischemia brain,which is related to improve the learning and memory via regulating energy metabolism and anti-oxidation.
文摘为明确影响鹅绒藤分布的主导环境因子及其生态阈值,量化不同时期其潜在适生区,借助MaxEnt模型与ArcGIS空间分析技术,融合鹅绒藤在我国的301个分布点位信息及生物气候数据,对其在2个过去时期[末次盛冰期(约22000 a前)和全新世中期(约6000 a前)]、当前时期(1970—2000年)以及3个未来时期[2030s(2021—2040年)、2050s(2041—2060年)、2070s(2061—2080年)]的潜在适生区进行预测分析。结果表明,MaxEnt模型各时期受试者工作特征曲线下面积(Area under the curve,AUC)值均高于0.9,预测结果可靠。鹅绒藤的分布主要受年平均温度、温度季节性变异系数、降水量季节性变异系数和最湿月份降水量影响。当前时期下,鹅绒藤的总适生区面积达193.37675×10^(4)km^(2),其中高适生区面积为69.01563×10^(4)km^(2)。与当前时期相对比,鹅绒藤在末次盛冰期和全新世中期适生区扩张幅度较为显著。3个未来时期中,鹅绒藤在SSP370(区域竞争路径)气候变化情景下2070s适生区面积最大,在SSP126(可持续路径)气候变化情景下2030s适生区面积最小,但均比当前时期适生区面积有所扩张。综上,鹅绒藤在2个过去时期和3个未来时期不同气候情景下均呈现扩张趋势,反映了气候变化,尤其是碳循环所引起的温度变化对物种分布的阶段性影响。
