With the rising and spreading of micro-blog, the sentiment classification of short texts has become a research hotspot. Some methods have been developed in the past decade. However, since the Chinese and English are d...With the rising and spreading of micro-blog, the sentiment classification of short texts has become a research hotspot. Some methods have been developed in the past decade. However, since the Chinese and English are different in language syntax, semantics and pragmatics, sentiment classification methods that are effective for English twitter may fail on Chinese micro-blog. In addition, the colloquialism and conciseness of short Chinese texts introduces additional challenges to sentiment classification. In this work, a novel hybrid learning model was proposed for sentiment classification of Chinese micro-blogs, which included two stages. In the first stage, emotional scores were calculated over the whole dataset by utilizing an improved Chinese-oriented sentiment dictionary classification method. Data with extremely high or low scores were directly labeled. In the second stage, the remaining data were labeled by using an integrated classification method based on sentiment dictionary, support vector machine(SVM) and k-nearest neighbor(KNN). An improved feature selection method was adopted to enhance the discriminative power of the selected features. The two-stage hybrid framework made the proposed method effective for sentiment classification of Chinese micro-blogs. Experiments on the COAE2014(Chinese Opinion Analysis Evaluation 2014) dataset show that the proposed method outperforms other schemes.展开更多
Objective:Keratoconus(KC)is a progressive corneal ectasia disorder,arising from a myriad of causes including genetic predispositions,environmental factors,biomechanical influences,and inflammatory reactions.This study...Objective:Keratoconus(KC)is a progressive corneal ectasia disorder,arising from a myriad of causes including genetic predispositions,environmental factors,biomechanical influences,and inflammatory reactions.This study aims to identify potential pathogenetic gene mutations in patients with sporadic KC in the Han Chinese population.Methods:Twenty-five patients with primary KC as well as 50 unrelated population matched healthy controls,were included in this study to identify potential pathogenic gene mutations among sporadic KC patients in the Han Chinese population.Sanger sequencing and whole-exome sequencing(WES)were used to analyze mutations in the zinc finger protein 469(ZNF469)gene.Bioinformatics analysis was conducted to explore the potential role of ZNF469 in KC pathogenesis.Results:Five novel heterozygous missense variants were identified in KC patients.Among them,2 compound heterozygous variants,c.8986G>C(p.E2996Q)with c.11765A>C(p.D3922A),and c.4423C>G(p.L1475V)with c.10633G>A(p.G3545R),were determined to be possible pathogenic factors for KC.Conclusion:Mutations in the ZNF469 gene may contribute to the development of KC in the Han Chinese population.These mutation sites may provide valuable information for future genetic screening of KC patients and their families.展开更多
OBJECTIVE The emergence of evolving variants of Coronavirus disease 2019(COVID-19)has fostered the need for change of newer and adaptive treatments for these infections.During the COVID-19 pandemic and persists,tradit...OBJECTIVE The emergence of evolving variants of Coronavirus disease 2019(COVID-19)has fostered the need for change of newer and adaptive treatments for these infections.During the COVID-19 pandemic and persists,traditional Chinese medicine(TCM)herbs exhibit significant bioactivity and therapeutic effect.This study is aimed to evaluate the efficacy of four TCM preparations on 28-day mortality risk of patients and changes of the laboratory indicators.METHODS The retrospective cohort study included patients with COVID-19 who were admitted to the Jiangsu Province Hospital of Chinese Medicine from December 15,2022 to January 15,2023,and those died within 48 hours of admission or cannot be tracked for outcomes were excluded.The primary outcome was survival status in 28 days(death or survival)starting from the day of admission.The second outcomes were laboratory indicators,including absolute lymphocyte count,lactate dehydrogenase,creatinine,and blood urea nitrogen.Binary logistic regressions were used to estimate the effect of TCM preparations on the primary and secondary outcomes in main analysis.Meanwhile,heterogeneity and robustness of results from main analysis were assessed by subgroup analyses and multiple sensitivity analyses.RESULTS 1816 eligible patients were included in analysis dataset,including 573 patients received standard care(control group)and 1243 patients received TCM preparations(hospital preparation group).The 28-day mortality rate of hospital preparation group was lower than that of control group(4.75%vs.14.83%),and the difference was statistically significant(χ^(2)=54.666,P<0.001).The risk of 28-day mortality was 0.535 times lower in the hospital preparation group as compared with the control group(OR=0.46,95%CI:0.305-0.708,P<0.001)showed by multivariable binary logistic regressions.Subgroup analyses showed that taking TCM preparations reduced the 28-day mortality risk.Sensitivity analyses demonstrated that the results of the main analysis for primary outcomes were robust.For secondary outcomes,the risk of abnormal absolute lymphocyte counts at discharge in the hospital preparation group decreased by 0.284 times(OR=0.703,95%CI:0.515-0.961,P=0.027).CONCLUSION Compared with standard of care,taking four hospital preparations including Kanggan Heji,Feining Heji,Qishen Gubiao Keli,and Qianghuo Qushi Qingwen Heji decreased risk of 28-day mortality among hospitalized COVID-19 patients.TCM therapy achieves adequate therapeutic effects in COVID-19.展开更多
As an international language, English is now spoken by more and more people as a first, second or foreign language. The Chinese students tends to ignore the American culture when they are learning the language of Engl...As an international language, English is now spoken by more and more people as a first, second or foreign language. The Chinese students tends to ignore the American culture when they are learning the language of English. This paper puts forward the view that teaching American culture plays a significant role in helping the Chinese students learn English and the English teachers have the responsibility to teach American culture to Chinese students.展开更多
OBJECTIVE Major depressive disorder(MDD) is a highly heterogeneous mental illness.Further classification may help characterize its heterogeneity.The purpose of this study was to examine metabolomic and brain connectom...OBJECTIVE Major depressive disorder(MDD) is a highly heterogeneous mental illness.Further classification may help characterize its heterogeneity.The purpose of this study was to examine metabolomic and brain connectomic associations with traditional Chinese medicine(TCM) diagnostic classification of MDD.METHODS Fifty unmedicated depressed patients were classified into Liver Qi Stagnation(LQS,n=30) and Heart and Spleen Deficiency(HSD,n=20) subtypes according to TCM diagnosis.Healthy volunteers(n=28) were included as controls.Gas chromatography-mass spectrometry(GC-MS) and diffusion tensor imaging were used to detect serum and urinary metabolomic profiles and whole-brain white matter connectivity,respectively.RESULTS In metabolomic analysis,28 metabolites were identified for good separations between TCM subtypes and healthy controls in serum and urine samples.While both TCM subtypes had similar profiles in proteinogenic branched-chain amino acids and energy metabolism-related metabolites that were differentiated from healthy controls,the LQS subtype additionally differed from healthy controls in multiple amino acid metabolites that are involved in the biosynthesis of monoamine and amino acid neurotransmitters.Several metabolites are differentially associated with the two subtypes.In connectomic analysis,The LQS subtype showed significant differences in multiple network metrics of the angular gyrus,middle occipital gyrus,calcarine sulcus,and Heschl′ s gyrus when compared to the other two groups.The HSD subtype had markedly greater regional connectivity of the insula,parahippocampal gyrus,and posterior cingulate gyrus than the other two groups,and microstructural abnormalities of the frontal medial orbital gyrus and middle temporal pole.The insular betweenness centrality was strongly inversely correlated with the severity of depression and dichotomized the two subtypes at the optimal cutoff value with acceptable sensitivity and specificity.CONCLUSION The LQS subtype may represent an MDD subpopulation mainly characterized by abnormalities in the biosynthesis of monoamine and amino acid neurotransmitters,closer associations with stress-related pathophysiology,and aberrant connectivity of the audiovisual perception-related temporal-occipital network,whereas the HSD subtype is more closely associated with hyperconnectivity and microstructural abnormalities of the limbicparalimbic network.Certain metabolomic and connectomic variables are potential biomarkers for TCM diagnostic subtypes which is perhaps an alternative classification for depressive disorders.展开更多
An experiment was conducted to study the effects of different Chinese herbs on lactation in the rat. Seventy-two Sprague Dawley female rats at gestation day 18 were assigned to 9 groups randomly, with 8 rats each. Con...An experiment was conducted to study the effects of different Chinese herbs on lactation in the rat. Seventy-two Sprague Dawley female rats at gestation day 18 were assigned to 9 groups randomly, with 8 rats each. Control rats were offered basal diets until the end of the weaning period. Rats in the other 8 groups were administered diets supplemented with 1% Medulla tetmpanacis （MT）, Astragalus membranacens （AM）, Vaccaria segetalis （VS）, Leonurus heterophyllus sweet （LHS）, Radix rehmarmiae preparata （RRP）, Squama man/t/s （SM）, Schisandra chinensis （SC）, Ligustrum lucidum （LL）, respectively. After parturition, the litter size was culled to 8 pups per dam. Pup weight and milk yield were significantly higher in the VS, LHS, AM and SM groups than these in the control group during middle and late lactation （P〈0.05 or P〈0.01）. The weight and feed intake were not different in the dams fed either diet.展开更多
The treatment and signaling pathway regulation effects of kidney-tonifying traditional Chinese medicine on osteoporosis have been widely studied,but without a systematic summary currently.This review comprehensively c...The treatment and signaling pathway regulation effects of kidney-tonifying traditional Chinese medicine on osteoporosis have been widely studied,but without a systematic summary currently.This review comprehensively collected and analyzed the traditional Chinese medicine on the treatment and signaling pathway regulation of osteoporosis in recent ten years,such as Epimedium,Drynariae Rhizoma,Cnidium,Eucommia,Psoralen and Dipsacus.Based on the existing findings,we concluded the following conclusions:(1)kidney-tonifying traditional Chinese medicine treats osteoporosis mainly through BMP-Smads,Wnt/β-catenin,MAPK,PI3K/AKT signaling pathway to promote osteoblast bone formation and through OPG/RANKL/RANK,estrogen,CTSK signaling pathway to inhibit osteoclasts of bone resorption.(1)Epimedium,Drynariae Rhizoma,Cnidium and Psoralen up-regulate the key proteins and genes of BMP-Smads and Wnt/β-catenin signaling pathways to promote bone formation.(2)Epimedium,Drynariae Rhizoma,Cnidium,Eucommia,Psoralen,Dipsacusinhibit the bone resorption by mediating the OPG/RANKL/RANK signaling pathway.(2)Kidney-tonifying traditional Chinese medicine prevent and treat osteoporosis through a variety of ways:Icariin,Naringin,Osthol,Psoralen can regulate BMP-Smads,Wnt/β-catenin signaling pathway to promote bone formation,but also activate OPG/RANKL/RANK,CTSK and other signaling pathway to inhibit bone resorption.(3)The crosstalk of the signaling pathways and the animal experiments of the traditional Chinese medicine on the prevention and treatment of osteoporosis as well as their multi-target mechanism and comprehensive regulation need further clarification.展开更多
The sharp increase of the amount of Internet Chinese text data has significantly prolonged the processing time of classification on these data.In order to solve this problem,this paper proposes and implements a parall...The sharp increase of the amount of Internet Chinese text data has significantly prolonged the processing time of classification on these data.In order to solve this problem,this paper proposes and implements a parallel naive Bayes algorithm(PNBA)for Chinese text classification based on Spark,a parallel memory computing platform for big data.This algorithm has implemented parallel operation throughout the entire training and prediction process of naive Bayes classifier mainly by adopting the programming model of resilient distributed datasets(RDD).For comparison,a PNBA based on Hadoop is also implemented.The test results show that in the same computing environment and for the same text sets,the Spark PNBA is obviously superior to the Hadoop PNBA in terms of key indicators such as speedup ratio and scalability.Therefore,Spark-based parallel algorithms can better meet the requirement of large-scale Chinese text data mining.展开更多
Chinese mitten crab （Eriocheir sinensis） is an indigenous and ecologically and economically important species in the Liao River area, but its identification and genetic diversity remain poorly understood. To evaluat...Chinese mitten crab （Eriocheir sinensis） is an indigenous and ecologically and economically important species in the Liao River area, but its identification and genetic diversity remain poorly understood. To evaluate the germplasm resources of this species, samples were collected from these locations： four sub-populations from the Liao River area and one population from the Yangtze River area; one primer was used to distinguish between the Liao River and the Yangtze River crabs. Thirteen loci were used for crab genetic diversity analysis, and basic statistics showed that the collecting samples were purebred in the Liao River area. The average observed heterozygosity （H0） of the Liao River population was 0.5931, and the expected heterozygosity （He） was 0.8064. The polymorphism information content （PIC） was 0.7753, which showed that the Liao River population had high genetic diversity. The genetic differentiation index （FST） averaged 0.0342, meaning a low degree of differentiation; cluster analysis indicated that Hujia （HJ）, Xinli （XL） and Chenjia （CJ） sub-populations were allocated to the same cluster, while Baqiangzi （BQZ） sub-population was isolated. In summary, these data demonstrated that the crabs in the Liao River had high genetic diversity, but low genetic differentiation. Thus, the Liao River population had the potential for breeding selection. Furthermore, this study also provided valuable genetic information for the conservation of Chinese mitten crab.展开更多
基金Projects(61573380,61303185)supported by the National Natural Science Foundation of ChinaProject(13BTQ052)supported by the National Social Science Foundation of China+1 种基金Project(2016M592450)supported by the China Postdoctoral Science FoundationProject(2016JJ4119)supported by the Hunan Provincial Natural Science Foundation of China
文摘With the rising and spreading of micro-blog, the sentiment classification of short texts has become a research hotspot. Some methods have been developed in the past decade. However, since the Chinese and English are different in language syntax, semantics and pragmatics, sentiment classification methods that are effective for English twitter may fail on Chinese micro-blog. In addition, the colloquialism and conciseness of short Chinese texts introduces additional challenges to sentiment classification. In this work, a novel hybrid learning model was proposed for sentiment classification of Chinese micro-blogs, which included two stages. In the first stage, emotional scores were calculated over the whole dataset by utilizing an improved Chinese-oriented sentiment dictionary classification method. Data with extremely high or low scores were directly labeled. In the second stage, the remaining data were labeled by using an integrated classification method based on sentiment dictionary, support vector machine(SVM) and k-nearest neighbor(KNN). An improved feature selection method was adopted to enhance the discriminative power of the selected features. The two-stage hybrid framework made the proposed method effective for sentiment classification of Chinese micro-blogs. Experiments on the COAE2014(Chinese Opinion Analysis Evaluation 2014) dataset show that the proposed method outperforms other schemes.
基金supported by the National Natural Science Foundation(82271057)the Natural Science Foundation of Hunan Province(2023JJ30818),China。
文摘Objective:Keratoconus(KC)is a progressive corneal ectasia disorder,arising from a myriad of causes including genetic predispositions,environmental factors,biomechanical influences,and inflammatory reactions.This study aims to identify potential pathogenetic gene mutations in patients with sporadic KC in the Han Chinese population.Methods:Twenty-five patients with primary KC as well as 50 unrelated population matched healthy controls,were included in this study to identify potential pathogenic gene mutations among sporadic KC patients in the Han Chinese population.Sanger sequencing and whole-exome sequencing(WES)were used to analyze mutations in the zinc finger protein 469(ZNF469)gene.Bioinformatics analysis was conducted to explore the potential role of ZNF469 in KC pathogenesis.Results:Five novel heterozygous missense variants were identified in KC patients.Among them,2 compound heterozygous variants,c.8986G>C(p.E2996Q)with c.11765A>C(p.D3922A),and c.4423C>G(p.L1475V)with c.10633G>A(p.G3545R),were determined to be possible pathogenic factors for KC.Conclusion:Mutations in the ZNF469 gene may contribute to the development of KC in the Han Chinese population.These mutation sites may provide valuable information for future genetic screening of KC patients and their families.
文摘OBJECTIVE The emergence of evolving variants of Coronavirus disease 2019(COVID-19)has fostered the need for change of newer and adaptive treatments for these infections.During the COVID-19 pandemic and persists,traditional Chinese medicine(TCM)herbs exhibit significant bioactivity and therapeutic effect.This study is aimed to evaluate the efficacy of four TCM preparations on 28-day mortality risk of patients and changes of the laboratory indicators.METHODS The retrospective cohort study included patients with COVID-19 who were admitted to the Jiangsu Province Hospital of Chinese Medicine from December 15,2022 to January 15,2023,and those died within 48 hours of admission or cannot be tracked for outcomes were excluded.The primary outcome was survival status in 28 days(death or survival)starting from the day of admission.The second outcomes were laboratory indicators,including absolute lymphocyte count,lactate dehydrogenase,creatinine,and blood urea nitrogen.Binary logistic regressions were used to estimate the effect of TCM preparations on the primary and secondary outcomes in main analysis.Meanwhile,heterogeneity and robustness of results from main analysis were assessed by subgroup analyses and multiple sensitivity analyses.RESULTS 1816 eligible patients were included in analysis dataset,including 573 patients received standard care(control group)and 1243 patients received TCM preparations(hospital preparation group).The 28-day mortality rate of hospital preparation group was lower than that of control group(4.75%vs.14.83%),and the difference was statistically significant(χ^(2)=54.666,P<0.001).The risk of 28-day mortality was 0.535 times lower in the hospital preparation group as compared with the control group(OR=0.46,95%CI:0.305-0.708,P<0.001)showed by multivariable binary logistic regressions.Subgroup analyses showed that taking TCM preparations reduced the 28-day mortality risk.Sensitivity analyses demonstrated that the results of the main analysis for primary outcomes were robust.For secondary outcomes,the risk of abnormal absolute lymphocyte counts at discharge in the hospital preparation group decreased by 0.284 times(OR=0.703,95%CI:0.515-0.961,P=0.027).CONCLUSION Compared with standard of care,taking four hospital preparations including Kanggan Heji,Feining Heji,Qishen Gubiao Keli,and Qianghuo Qushi Qingwen Heji decreased risk of 28-day mortality among hospitalized COVID-19 patients.TCM therapy achieves adequate therapeutic effects in COVID-19.
文摘As an international language, English is now spoken by more and more people as a first, second or foreign language. The Chinese students tends to ignore the American culture when they are learning the language of English. This paper puts forward the view that teaching American culture plays a significant role in helping the Chinese students learn English and the English teachers have the responsibility to teach American culture to Chinese students.
基金National Natural Science Foundation of China(81403502)General Research Fund ofResearch Grants Council of Hong Kong (17124418).
文摘OBJECTIVE Major depressive disorder(MDD) is a highly heterogeneous mental illness.Further classification may help characterize its heterogeneity.The purpose of this study was to examine metabolomic and brain connectomic associations with traditional Chinese medicine(TCM) diagnostic classification of MDD.METHODS Fifty unmedicated depressed patients were classified into Liver Qi Stagnation(LQS,n=30) and Heart and Spleen Deficiency(HSD,n=20) subtypes according to TCM diagnosis.Healthy volunteers(n=28) were included as controls.Gas chromatography-mass spectrometry(GC-MS) and diffusion tensor imaging were used to detect serum and urinary metabolomic profiles and whole-brain white matter connectivity,respectively.RESULTS In metabolomic analysis,28 metabolites were identified for good separations between TCM subtypes and healthy controls in serum and urine samples.While both TCM subtypes had similar profiles in proteinogenic branched-chain amino acids and energy metabolism-related metabolites that were differentiated from healthy controls,the LQS subtype additionally differed from healthy controls in multiple amino acid metabolites that are involved in the biosynthesis of monoamine and amino acid neurotransmitters.Several metabolites are differentially associated with the two subtypes.In connectomic analysis,The LQS subtype showed significant differences in multiple network metrics of the angular gyrus,middle occipital gyrus,calcarine sulcus,and Heschl′ s gyrus when compared to the other two groups.The HSD subtype had markedly greater regional connectivity of the insula,parahippocampal gyrus,and posterior cingulate gyrus than the other two groups,and microstructural abnormalities of the frontal medial orbital gyrus and middle temporal pole.The insular betweenness centrality was strongly inversely correlated with the severity of depression and dichotomized the two subtypes at the optimal cutoff value with acceptable sensitivity and specificity.CONCLUSION The LQS subtype may represent an MDD subpopulation mainly characterized by abnormalities in the biosynthesis of monoamine and amino acid neurotransmitters,closer associations with stress-related pathophysiology,and aberrant connectivity of the audiovisual perception-related temporal-occipital network,whereas the HSD subtype is more closely associated with hyperconnectivity and microstructural abnormalities of the limbicparalimbic network.Certain metabolomic and connectomic variables are potential biomarkers for TCM diagnostic subtypes which is perhaps an alternative classification for depressive disorders.
基金Supported by NationalBasicResearchProgramof China(2004CB11750-5)
文摘An experiment was conducted to study the effects of different Chinese herbs on lactation in the rat. Seventy-two Sprague Dawley female rats at gestation day 18 were assigned to 9 groups randomly, with 8 rats each. Control rats were offered basal diets until the end of the weaning period. Rats in the other 8 groups were administered diets supplemented with 1% Medulla tetmpanacis （MT）, Astragalus membranacens （AM）, Vaccaria segetalis （VS）, Leonurus heterophyllus sweet （LHS）, Radix rehmarmiae preparata （RRP）, Squama man/t/s （SM）, Schisandra chinensis （SC）, Ligustrum lucidum （LL）, respectively. After parturition, the litter size was culled to 8 pups per dam. Pup weight and milk yield were significantly higher in the VS, LHS, AM and SM groups than these in the control group during middle and late lactation （P〈0.05 or P〈0.01）. The weight and feed intake were not different in the dams fed either diet.
文摘The treatment and signaling pathway regulation effects of kidney-tonifying traditional Chinese medicine on osteoporosis have been widely studied,but without a systematic summary currently.This review comprehensively collected and analyzed the traditional Chinese medicine on the treatment and signaling pathway regulation of osteoporosis in recent ten years,such as Epimedium,Drynariae Rhizoma,Cnidium,Eucommia,Psoralen and Dipsacus.Based on the existing findings,we concluded the following conclusions:(1)kidney-tonifying traditional Chinese medicine treats osteoporosis mainly through BMP-Smads,Wnt/β-catenin,MAPK,PI3K/AKT signaling pathway to promote osteoblast bone formation and through OPG/RANKL/RANK,estrogen,CTSK signaling pathway to inhibit osteoclasts of bone resorption.(1)Epimedium,Drynariae Rhizoma,Cnidium and Psoralen up-regulate the key proteins and genes of BMP-Smads and Wnt/β-catenin signaling pathways to promote bone formation.(2)Epimedium,Drynariae Rhizoma,Cnidium,Eucommia,Psoralen,Dipsacusinhibit the bone resorption by mediating the OPG/RANKL/RANK signaling pathway.(2)Kidney-tonifying traditional Chinese medicine prevent and treat osteoporosis through a variety of ways:Icariin,Naringin,Osthol,Psoralen can regulate BMP-Smads,Wnt/β-catenin signaling pathway to promote bone formation,but also activate OPG/RANKL/RANK,CTSK and other signaling pathway to inhibit bone resorption.(3)The crosstalk of the signaling pathways and the animal experiments of the traditional Chinese medicine on the prevention and treatment of osteoporosis as well as their multi-target mechanism and comprehensive regulation need further clarification.
基金Project(KC18071)supported by the Application Foundation Research Program of Xuzhou,ChinaProjects(2017YFC0804401,2017YFC0804409)supported by the National Key R&D Program of China
文摘The sharp increase of the amount of Internet Chinese text data has significantly prolonged the processing time of classification on these data.In order to solve this problem,this paper proposes and implements a parallel naive Bayes algorithm(PNBA)for Chinese text classification based on Spark,a parallel memory computing platform for big data.This algorithm has implemented parallel operation throughout the entire training and prediction process of naive Bayes classifier mainly by adopting the programming model of resilient distributed datasets(RDD).For comparison,a PNBA based on Hadoop is also implemented.The test results show that in the same computing environment and for the same text sets,the Spark PNBA is obviously superior to the Hadoop PNBA in terms of key indicators such as speedup ratio and scalability.Therefore,Spark-based parallel algorithms can better meet the requirement of large-scale Chinese text data mining.
基金Supported by Open Project Program of Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture,Heilongjiang Fisheries Research Institute,Chinese Academy of Fishery Sciences(FBB2016-01)
文摘Chinese mitten crab （Eriocheir sinensis） is an indigenous and ecologically and economically important species in the Liao River area, but its identification and genetic diversity remain poorly understood. To evaluate the germplasm resources of this species, samples were collected from these locations： four sub-populations from the Liao River area and one population from the Yangtze River area; one primer was used to distinguish between the Liao River and the Yangtze River crabs. Thirteen loci were used for crab genetic diversity analysis, and basic statistics showed that the collecting samples were purebred in the Liao River area. The average observed heterozygosity （H0） of the Liao River population was 0.5931, and the expected heterozygosity （He） was 0.8064. The polymorphism information content （PIC） was 0.7753, which showed that the Liao River population had high genetic diversity. The genetic differentiation index （FST） averaged 0.0342, meaning a low degree of differentiation; cluster analysis indicated that Hujia （HJ）, Xinli （XL） and Chenjia （CJ） sub-populations were allocated to the same cluster, while Baqiangzi （BQZ） sub-population was isolated. In summary, these data demonstrated that the crabs in the Liao River had high genetic diversity, but low genetic differentiation. Thus, the Liao River population had the potential for breeding selection. Furthermore, this study also provided valuable genetic information for the conservation of Chinese mitten crab.
