OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The mode...OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate(250 mg·kg^(-1))for 7 d.Water extracts of Wudang cherry at 500 mg·kg^(-1)were orally administered to hyperuricemic mice for 7 d,benzbromarone(20 mg·kg^(-1))and allopurinol(20 mg·kg^(-1))were given as positive controls,vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice.Simultaneously,the m RNA and protein levels of mouse urate transporter 1(m URAT1),glucose transporter 9(mGLUT9),organic anion transporters(mOAT1 and mOAT3),ATP-binding cassette,subfamily G,membrane 2(mABCG2)and organic cation/carnitine transporters(m OCT1,m OCT2,m OCTN1 and m OCTN2)in the kidney were analyzed by Western blot,RT-PCR,immunohistochemical and immunofluorescent assay,respectively.RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice.And it effectively reversed potassium oxonate-induced alterations in renal m URAT1,mGLUT9,mOAT1,mOAT3 and mABCG2 m RNA and protein levels,resulting in the enhancement of renal urate excretion in mice.Moreover,Wudang Cherry increased renal m OCT1,m OCT2,m OCTN1 and m OCTN2 m RNA and protein levels,and improved renal impairment in this model.CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.展开更多
This objective of the study was to develop a model for the determination of N deficiency in cherry trees using a combination of visible near infrared methods and spectro-radiometric measurement.In our experimental des...This objective of the study was to develop a model for the determination of N deficiency in cherry trees using a combination of visible near infrared methods and spectro-radiometric measurement.In our experimental design,cherry seedlings were grown under various N deficiency conditions in nutrient-controlled containers.The reflectance values of plant leaves were measured using a spectro-radiometer.Plant leaves samples were simultaneously collected.Their nutrient contents were determined in the laboratory.Afterwards,we performed a statistical comparison of the reflectance values.Sample analysis results established the significant wavelengths.Moreover,we received accurate regression models for predicting N deficiency in cherry leaves that were grown in nutrient solutions.Next,we verified the model validity by measuring the reflectance of the leaves collected from cherry orchards at various locations using a spectroradiometer.Nutrient deficiencies were calculated using the developed model,and then,the predicted and measured data were compared to evaluate model validity.From these results,we determined the wavelengths that yielded the most accurate results for N prediction,selected from the blue and green regions of the spectrum.We established that for N prediction in cherry trees,the simplest model can be created using 560and 570nm wavelengths.However,the evaluated model can be applicable only under certain conditions.We concluded that in order to develop aprediction method with sufficient application capacity,as well as the ability to assess nutritional and physiological characteristics,the ecology condition of the plant should be properly considered based on the model.展开更多
Visible and near-infrared(VNIR)spectroscopy is an eco-friendly method used for estimating plant nutrient deficiencies.The aim of this study was to investigate the possibility of using VNIR method for estimating Zn con...Visible and near-infrared(VNIR)spectroscopy is an eco-friendly method used for estimating plant nutrient deficiencies.The aim of this study was to investigate the possibility of using VNIR method for estimating Zn content in cherry orchard leaves under field conditions.The study was conducted in 3different locations in Isparta region of Turkey.Fifteen cherry orchards containing normal and Zn deficient plants were chosen,and 60 leaf samples were collected from each location.The reflectance spectra of the leaves were measured with an ASD FieldSpec HandHeld spectroradiometer and a plant probe.The Zn contents of leaf samples were predicted through laboratory analysis.The spectral reflectance measurements were used to estimate the Zn levels using stepwise multiple linear regression analysis method.Prediction models were created using the highest coefficient of determination value.The results show that Zn content of cherry trees can be estimated using the VNIR spectroscopic method(87.5<r2<96.79).Moreover,plant nutrient contents can be estimated without using chemicals.However,further research is necessary to develop a standard method for field conditions.Because spectral reflectance is affected by ecological conditions,agricultural applications and nutrient interactions,more effective models must be developed depending on the geographical location,period and plant type.展开更多
We have conducted some studies on 3 ground cherry cultivars'Artaskaja'.'Bushkaja'and'Maksmorfikaja'introduced from Russia.The results indicated that these cultivars could survive temperature -4...We have conducted some studies on 3 ground cherry cultivars'Artaskaja'.'Bushkaja'and'Maksmorfikaja'introduced from Russia.The results indicated that these cultivars could survive temperature -40℃ during severe winter in Harbin,China and had high drought and disease resistance,the average weight of each fruit reached 2.78g,the contents of Vc and soluble solid exceeded 30mg/100g and 12%,respectively.Not only does the fruits suit for fresh market,but also for processing.Ground cherry is a promising new fruit crop in cold region.展开更多
基金supported by National Natural Science Foundation of China(81402994)Natural Science Foundation of Hubei Province(2014CFB652)"2011 Plan" Open Fund Innovation Team Project of Hubei Province(2011JH-2014CXTT08)
文摘OBJECTIVE To investigate effects of Wudang cherry on urate excretion and renal function and examined whether renal organic ion transporters were involved in potassium oxonateinduced hyperuricemic mice.METHODS The model of hyperuricemic mice was induced by intraperitoneal injection of potassium oxonate(250 mg·kg^(-1))for 7 d.Water extracts of Wudang cherry at 500 mg·kg^(-1)were orally administered to hyperuricemic mice for 7 d,benzbromarone(20 mg·kg^(-1))and allopurinol(20 mg·kg^(-1))were given as positive controls,vehicle control group was given equal normal saline.Serum and urine levels of uric acid were measured in hyperuricemic and normal mice.Simultaneously,the m RNA and protein levels of mouse urate transporter 1(m URAT1),glucose transporter 9(mGLUT9),organic anion transporters(mOAT1 and mOAT3),ATP-binding cassette,subfamily G,membrane 2(mABCG2)and organic cation/carnitine transporters(m OCT1,m OCT2,m OCTN1 and m OCTN2)in the kidney were analyzed by Western blot,RT-PCR,immunohistochemical and immunofluorescent assay,respectively.RESULTS Wudang cherry significantly reduced serum uric acid levels and increased urine uric acid levels in hyperuricemic mice.And it effectively reversed potassium oxonate-induced alterations in renal m URAT1,mGLUT9,mOAT1,mOAT3 and mABCG2 m RNA and protein levels,resulting in the enhancement of renal urate excretion in mice.Moreover,Wudang Cherry increased renal m OCT1,m OCT2,m OCTN1 and m OCTN2 m RNA and protein levels,and improved renal impairment in this model.CONCLUSION Wudang cherry processes uricosuric and nephroprotective actions by regulating renal organic ion transporters in hyperuricemic mice.
基金The Scientific and Technical Research Council of Turkey,Agriculture,Forestry&Veterinary Research Grant Committee Project No TOVAG 109O376
文摘This objective of the study was to develop a model for the determination of N deficiency in cherry trees using a combination of visible near infrared methods and spectro-radiometric measurement.In our experimental design,cherry seedlings were grown under various N deficiency conditions in nutrient-controlled containers.The reflectance values of plant leaves were measured using a spectro-radiometer.Plant leaves samples were simultaneously collected.Their nutrient contents were determined in the laboratory.Afterwards,we performed a statistical comparison of the reflectance values.Sample analysis results established the significant wavelengths.Moreover,we received accurate regression models for predicting N deficiency in cherry leaves that were grown in nutrient solutions.Next,we verified the model validity by measuring the reflectance of the leaves collected from cherry orchards at various locations using a spectroradiometer.Nutrient deficiencies were calculated using the developed model,and then,the predicted and measured data were compared to evaluate model validity.From these results,we determined the wavelengths that yielded the most accurate results for N prediction,selected from the blue and green regions of the spectrum.We established that for N prediction in cherry trees,the simplest model can be created using 560and 570nm wavelengths.However,the evaluated model can be applicable only under certain conditions.We concluded that in order to develop aprediction method with sufficient application capacity,as well as the ability to assess nutritional and physiological characteristics,the ecology condition of the plant should be properly considered based on the model.
文摘Visible and near-infrared(VNIR)spectroscopy is an eco-friendly method used for estimating plant nutrient deficiencies.The aim of this study was to investigate the possibility of using VNIR method for estimating Zn content in cherry orchard leaves under field conditions.The study was conducted in 3different locations in Isparta region of Turkey.Fifteen cherry orchards containing normal and Zn deficient plants were chosen,and 60 leaf samples were collected from each location.The reflectance spectra of the leaves were measured with an ASD FieldSpec HandHeld spectroradiometer and a plant probe.The Zn contents of leaf samples were predicted through laboratory analysis.The spectral reflectance measurements were used to estimate the Zn levels using stepwise multiple linear regression analysis method.Prediction models were created using the highest coefficient of determination value.The results show that Zn content of cherry trees can be estimated using the VNIR spectroscopic method(87.5<r2<96.79).Moreover,plant nutrient contents can be estimated without using chemicals.However,further research is necessary to develop a standard method for field conditions.Because spectral reflectance is affected by ecological conditions,agricultural applications and nutrient interactions,more effective models must be developed depending on the geographical location,period and plant type.
文摘We have conducted some studies on 3 ground cherry cultivars'Artaskaja'.'Bushkaja'and'Maksmorfikaja'introduced from Russia.The results indicated that these cultivars could survive temperature -40℃ during severe winter in Harbin,China and had high drought and disease resistance,the average weight of each fruit reached 2.78g,the contents of Vc and soluble solid exceeded 30mg/100g and 12%,respectively.Not only does the fruits suit for fresh market,but also for processing.Ground cherry is a promising new fruit crop in cold region.
